Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,489 C→G 18.4% P457P (CCC→CCG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4890C→G18.4% 108.7 ‑2.4 ‑0.0241P457P (CCC→CCGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/23):  ref base (121/75):  total (142/99)
Fisher's exact test strand distribution p-value = 9.28e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.76e-01

Only 100 of 251 total aligned reads displayed.

TGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397389‑1397588
                                                                                                       |                                                                                                   
tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                                     >  2:2453137/1‑101 (MQ=255)
 ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                                    >  1:3658974/1‑101 (MQ=255)
 ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                                    >  2:2570419/1‑101 (MQ=255)
 ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                                    >  1:2649625/1‑101 (MQ=255)
 ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                                    <  1:1862253/101‑1 (MQ=255)
  gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTg                                                                                                   >  2:3684293/1‑101 (MQ=255)
   aTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGc                                                                                                  >  2:3501087/1‑101 (MQ=255)
    tagAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                                 <  1:4403631/99‑1 (MQ=255)
    tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                                 >  1:599753/1‑101 (MQ=255)
     cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                                                >  1:1174838/1‑101 (MQ=255)
     cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                                                >  2:805669/1‑101 (MQ=255)
      gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                                               <  1:4737428/101‑1 (MQ=255)
       aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                              >  2:3007338/1‑101 (MQ=255)
        aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcaccac                                                                                             >  1:2127539/1‑101 (MQ=255)
        aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcaccac                                                                                             <  1:468067/101‑1 (MQ=255)
         aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                            >  1:3465451/1‑101 (MQ=255)
          ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                           >  2:3104602/1‑101 (MQ=255)
             ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                        >  1:1143913/1‑101 (MQ=255)
               cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                                      >  2:2481955/1‑101 (MQ=255)
                 aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                    >  1:1696328/1‑101 (MQ=255)
                  tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTcc                                                                                   >  1:1292300/1‑101 (MQ=255)
                      tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                               <  2:4373413/101‑1 (MQ=255)
                       atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                              >  1:3268135/1‑101 (MQ=255)
                       atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                              <  2:860262/101‑1 (MQ=255)
                       atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                              <  1:822844/101‑1 (MQ=255)
                         ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TC.TC.AACCCGCAA.CCGCCCCGCACCCCCGGGCGCCTCCCCCCCgg                                                                           >  1:2762949/1‑101 (MQ=21)
                          gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgg                                                                           >  2:774519/1‑101 (MQ=255)
                                 cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  2:2946461/101‑1 (MQ=255)
                                 cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                                    >  1:1944511/1‑101 (MQ=255)
                                    cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   >  2:3767464/10‑101 (MQ=0)
                                    cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:520386/92‑1 (MQ=0)
                                    ccgcaccATGTCGCTCACCCCGCGGGGGCGTGGATCGGAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:1900126/95‑1 (MQ=37)
                                    ccGCGACATGTCGCTCTCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:1528627/101‑1 (MQ=255)
                                    ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:1020711/101‑1 (MQ=255)
                                    ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  1:1539629/1‑101 (MQ=255)
                                    ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGGGGAt                                                                 >  2:3663524/1‑101 (MQ=255)
                                    ccGCGACATGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:1589370/101‑1 (MQ=255)
                                     gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGa                                                                  >  1:479829/9‑101 (MQ=0)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:3891690/101‑1 (MQ=255)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:2660089/101‑1 (MQ=255)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:960085/1‑101 (MQ=255)
                                      gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:4492626/101‑1 (MQ=255)
                                      ataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCTCCCGCGGGGGCGTGGAt                                                                 >  2:3783012/8‑101 (MQ=0)
                                       cgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:1000454/1‑101 (MQ=255)
                                        accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:2069985/96‑1 (MQ=2)
                                         aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  1:4672675/1‑101 (MQ=255)
                                          cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                           <  1:243507/101‑1 (MQ=255)
                                          cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                           >  2:4058662/1‑101 (MQ=255)
                                          cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                           >  2:6882/1‑101 (MQ=255)
                                           gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  1:3124721/3‑101 (MQ=2)
                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaa                                                         >  2:1282712/1‑101 (MQ=255)
                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaa                                                         >  2:4246922/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaac                                                        >  2:4686575/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGCCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAcacaac                                                        >  2:2290384/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                                        <  1:883732/101‑4 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAaacggt                                                        >  2:4436945/1‑98 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                     <  1:3104803/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGa                                                    <  1:2535786/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggggtc                                                     >  2:3624334/1‑95 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                   >  1:4516962/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                   >  1:10871/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                   <  1:2724670/101‑9 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                   <  2:2327641/101‑9 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                   <  1:3502971/101‑9 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCa                                                 >  1:4256316/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCa                                                 >  2:1119777/1‑101 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                  <  2:2553251/101‑10 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                  <  1:720084/101‑10 (MQ=2)
                                                      cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaaagatcggaag                                               >  2:176320/1‑91 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                               <  1:2091823/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                             >  2:2056359/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACACCG.CCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                            <  2:2794916/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                           >  2:3392606/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                          >  2:197033/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGNNCGCCt                                       >  1:2216197/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                       >  2:1066458/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACACCG.CCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                      >  1:29844/1‑101 (MQ=255)
                                                                cGTGGATCGAAACACCG.TCATC.CCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                     <  1:2275203/101‑1 (MQ=255)
                                                                 gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                    >  1:1718644/1‑101 (MQ=255)
                                                                 gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                    >  2:3874729/1‑101 (MQ=255)
                                                                  tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                   >  2:1004692/1‑101 (MQ=255)
                                                                  tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                   >  2:240149/1‑101 (MQ=255)
                                                                         aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                            >  2:3320444/1‑101 (MQ=255)
                                                                         aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                            >  2:3157461/1‑101 (MQ=255)
                                                                             acCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCACCGCCGCGCTGAc                        >  1:3786/1‑101 (MQ=255)
                                                                               cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                      >  2:1574541/1‑101 (MQ=255)
                                                                                g.TCCTC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                     <  1:2453368/101‑1 (MQ=255)
                                                                                   catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                   >  2:4582071/1‑101 (MQ=255)
                                                                                    atc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                  >  2:252274/1‑101 (MQ=255)
                                                                                      c.accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                <  1:4629726/101‑1 (MQ=255)
                                                                                        accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc               <  1:329581/101‑1 (MQ=255)
                                                                                         ccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGATGTCGCTcccc              <  2:4672419/101‑1 (MQ=255)
                                                                                           acGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc            <  1:1403126/101‑1 (MQ=255)
                                                                                           acGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc            >  1:2841800/1‑101 (MQ=255)
                                                                                           acGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc            >  2:4005681/1‑101 (MQ=255)
                                                                                            cGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg           <  2:1696223/101‑1 (MQ=255)
                                                                                                  ccGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg      <  2:4099268/101‑1 (MQ=255)
                                                                                                    gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc    <  1:3663753/101‑1 (MQ=255)
                                                                                                    gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc    <  1:2570571/101‑1 (MQ=255)
                                                                                                      ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt  <  1:2920158/101‑1 (MQ=255)
                                                                                                       |                                                                                                   
TGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1397389‑1397588

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG