Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,553 A→C 8.3% S479R (AGC→CAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5530A→C 8.3% 46.7 ‑1.3 ‑0.0275S479R (AGC→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/13):  ref base (140/112):  total (150/125)
Fisher's exact test strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.76e-01

Only 100 of 287 total aligned reads displayed.

CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653
                                                                                                       |                                                                                                      
cGTGGATCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                                        <  1:2275203/101‑1 (MQ=255)
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACAACCCGATCATGGC.C..CGCCTCAg                                                                                                       >  2:3040432/1‑101 (MQ=255)
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                       >  1:3693404/1‑101 (MQ=255)
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                       >  1:1718644/1‑101 (MQ=255)
     aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                                   <  1:1106155/101‑1 (MQ=255)
        gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                                >  1:1068291/1‑101 (MQ=255)
               cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                                       >  2:1574541/1‑101 (MQ=255)
                gTCCTCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      <  1:2453368/101‑1 (MQ=255)
                gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      >  2:4057934/1‑101 (MQ=255)
                  catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCCCAGCGCCGCGCT.GA.CGTCGc                                                                                    >  2:4547437/1‑101 (MQ=255)
                   atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                   >  2:252274/1‑101 (MQ=255)
                    tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTc                                                                                  <  2:4479429/101‑1 (MQ=255)
                     caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                 >  1:4667190/1‑101 (MQ=255)
                      accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                <  1:329581/101‑1 (MQ=255)
                       ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.TGTCGCTcccc                                                                               <  2:4672419/101‑1 (MQ=255)
                         ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                             <  1:4735339/100‑1 (MQ=255)
                         acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                             <  1:1403126/101‑1 (MQ=255)
                            cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                          <  2:3751349/101‑1 (MQ=255)
                            cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                          >  2:3845235/1‑101 (MQ=255)
                             aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                         >  2:3283618/1‑101 (MQ=255)
                               ccGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                       <  1:1004771/101‑1 (MQ=255)
                                     tGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:2404256/101‑1 (MQ=255)
                                     tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:240174/101‑1 (MQ=255)
                                      tgccgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  2:4154619/92‑1 (MQ=2)
                                        atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:234407/94‑1 (MQ=0)
                                        atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:1152879/94‑1 (MQ=0)
                                        accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:2057097/101‑1 (MQ=255)
                                        accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:774593/101‑1 (MQ=255)
                                         cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:1561537/95‑1 (MQ=2)
                                         ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:2248834/101‑1 (MQ=255)
                                         ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:928142/101‑1 (MQ=255)
                                          cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:3916306/6‑101 (MQ=0)
                                          cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:1368405/96‑1 (MQ=0)
                                          caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  2:3233188/1‑101 (MQ=255)
                                          caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  1:4614040/1‑101 (MQ=255)
                                           gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:2917892/98‑1 (MQ=2)
                                            ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:4343928/99‑1 (MQ=2)
                                            agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:495057/4‑101 (MQ=2)
                                             tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:4581832/100‑1 (MQ=2)
                                              ggTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        <  2:633198/101‑1 (MQ=255)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        >  2:847278/1‑101 (MQ=2)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                                     <  1:4107686/101‑5 (MQ=2)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACaa                                                       >  1:4295948/1‑100 (MQ=2)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACaa                                                       >  1:297568/1‑100 (MQ=2)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                      <  1:3157661/101‑1 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                     <  1:4218422/101‑2 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                     >  1:4575518/1‑100 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:1252647/101‑3 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      >  1:2841537/1‑99 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                     >  2:4690384/1‑101 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                     >  1:3381608/1‑101 (MQ=255)
                                                 cGCGCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:80453/101‑3 (MQ=0)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATCGCAAcggggcg                                                   >  2:3392058/1‑95 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                    >  1:551825/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                    >  1:2625802/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                    >  2:3640152/1‑101 (MQ=255)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcggggcga                                                  >  1:153995/1‑94 (MQ=2)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGATCCAAAcggggcga                                                  >  1:2989985/1‑94 (MQ=0)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                   <  2:3923477/101‑4 (MQ=2)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATcgat                                                   >  2:2148083/1‑98 (MQ=2)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                    <  2:2640752/101‑5 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAcgggtcgaag                                                <  1:2942388/101‑10 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACCCCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                 <  1:3939855/101‑6 (MQ=0)
                                                     ccccccGCGGGGGCGTGGATCGAAACCCCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                 <  1:2873825/101‑6 (MQ=0)
                                                     ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                 <  2:3988013/101‑6 (MQ=2)
                                                      cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                 >  2:4235921/1‑94 (MQ=0)
                                                      cccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                                <  1:4509797/101‑1 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                               <  2:2467740/101‑1 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                               <  2:1034488/101‑1 (MQ=255)
                                                         ccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                             >  2:1839132/1‑101 (MQ=255)
                                                            cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                          >  2:1131965/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         >  2:2903093/1‑101 (MQ=255)
                                                                ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGTTCGGCGTGAc                                      >  2:1996682/1‑101 (MQ=255)
                                                                  cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                    >  1:3325730/1‑101 (MQ=255)
                                                                    tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                  >  2:3432981/1‑101 (MQ=255)
                                                                    tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                  >  1:2680367/1‑101 (MQ=255)
                                                                    tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                  <  1:3775916/101‑1 (MQ=255)
                                                                     ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccggc                                 >  2:3095300/1‑99 (MQ=255)
                                                                      gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                <  1:2757050/101‑1 (MQ=255)
                                                                      gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                <  2:1696601/101‑1 (MQ=255)
                                                                       aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                               >  2:4386180/1‑101 (MQ=255)
                                                                          gAAACAACCCGATCATGGC.C..CGCCTCCGCGCCGCGCT.GG.CGGCGCTCCCCCCGCGGGGGCGTGGATCAAAACATCCGCGGATCGGCGTGACcgggcccgcc                            >  2:1538542/1‑101 (MQ=37)
                                                                          gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACATCCGCGGATCGGCGTGACccgccccgcc                            >  1:4047866/1‑101 (MQ=255)
                                                                          gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                            >  1:360887/1‑101 (MQ=255)
                                                                           aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           >  1:820117/1‑101 (MQ=255)
                                                                           aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           <  1:1357517/101‑1 (MQ=255)
                                                                            aacaacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                          >  2:124529/1‑101 (MQ=255)
                                                                            aacaacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                          <  1:3640381/101‑1 (MQ=255)
                                                                               accccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                       <  1:685540/99‑1 (MQ=255)
                                                                                  ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    <  1:2239917/101‑1 (MQ=255)
                                                                                  ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    <  2:2990885/101‑1 (MQ=255)
                                                                                       cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc               >  2:1739118/1‑101 (MQ=255)
                                                                                        aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc              <  2:3693174/101‑1 (MQ=255)
                                                                                        aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc              <  1:1792569/101‑1 (MQ=255)
                                                                                          ggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc            >  1:3058687/1‑101 (MQ=255)
                                                                                           gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc           <  2:2841629/101‑1 (MQ=255)
                                                                                                   ccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg      <  1:1907665/101‑1 (MQ=255)
                                                                                                      cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc   <  2:131575/101‑1 (MQ=255)
                                                                                                      cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc   >  1:2136317/1‑101 (MQ=255)
                                                                                                       aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg  >  1:3843837/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG