Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,870 G→A 1.9% intergenic (+174/+1724) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8700G→A 1.9% 4.9 0.0 ‑0.1261intergenic (+174/+1724)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (122/134):  total (124/137)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.31e-01

Only 100 of 278 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397770‑1397970
                                                                                                    |                                                                                                    
cTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                                                                      <  1:1252021/101‑1 (MQ=255)
        gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCa                                                                                              <  1:942642/101‑1 (MQ=255)
        gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCa                                                                                              <  2:2684028/101‑1 (MQ=255)
         cgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAg                                                                                             <  2:2759215/101‑1 (MQ=255)
          gggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGc                                                                                            <  2:1770676/101‑1 (MQ=255)
           ggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGcc                                                                                           <  2:3720199/101‑1 (MQ=255)
            gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGccc                                                                                          >  1:875100/1‑101 (MQ=255)
            gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGccc                                                                                          <  1:298947/101‑1 (MQ=255)
            gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGccc                                                                                          <  1:2274900/101‑1 (MQ=255)
              gCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACCCGGCACCTCAGCcccg                                                                                        >  2:1828604/1‑101 (MQ=255)
               cGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgc                                                                                       >  2:2882109/1‑101 (MQ=255)
               cGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgc                                                                                       >  1:4476788/1‑101 (MQ=255)
                 tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgccc                                                                                     >  1:479779/1‑101 (MQ=255)
                  ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgcccg                                                                                    >  1:1420788/1‑101 (MQ=255)
                  ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCcccgcccg                                                                                    >  2:2120646/1‑101 (MQ=255)
                   gATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCccgcccgc                                                                                   >  1:1094530/1‑101 (MQ=255)
                       gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtc                                                                               >  1:3858341/1‑101 (MQ=255)
                        aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtct                                                                              >  1:4591079/1‑101 (MQ=255)
                         aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                             <  1:2912964/101‑1 (MQ=255)
                         aaCGGTATCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                             <  1:782584/101‑1 (MQ=255)
                          aCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCa                                                                            <  2:4320129/101‑1 (MQ=255)
                            ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAcc                                                                          >  2:752456/1‑101 (MQ=255)
                            ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAcc                                                                          >  2:3874512/1‑101 (MQ=255)
                             gtgtGCAGCGTCACATGCAGCGGCGGCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                         <  2:4753736/101‑1 (MQ=255)
                             gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                         <  2:3189228/101‑1 (MQ=255)
                             gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                         <  1:69049/101‑1 (MQ=255)
                             gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                         <  2:1337391/101‑1 (MQ=255)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                       <  1:2536102/101‑1 (MQ=255)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                       <  1:1353171/101‑1 (MQ=255)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                       >  1:3664245/1‑101 (MQ=255)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                       >  1:56736/1‑101 (MQ=255)
                                tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                      <  1:817992/101‑1 (MQ=255)
                                 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     <  2:204280/101‑1 (MQ=255)
                                 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     >  2:1036438/1‑101 (MQ=255)
                                 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     <  1:3217698/101‑1 (MQ=255)
                                 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     >  2:4177214/1‑101 (MQ=255)
                                   aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                   <  1:2120767/101‑1 (MQ=255)
                                   aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                   <  1:1828713/101‑1 (MQ=255)
                                    gCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCg                                                                  >  2:2940899/1‑101 (MQ=255)
                                     cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                 >  2:1932246/1‑101 (MQ=255)
                                        cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                              <  2:3086702/101‑1 (MQ=255)
                                        cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                              <  2:2392466/101‑1 (MQ=255)
                                        cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTcc                                                              >  2:2414708/1‑101 (MQ=255)
                                         acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                             <  1:286088/101‑1 (MQ=255)
                                          caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                            <  1:1794980/101‑1 (MQ=255)
                                           aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                           >  2:2346812/1‑101 (MQ=255)
                                           aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                           <  2:1264848/101‑1 (MQ=255)
                                            tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                          >  2:4549478/1‑101 (MQ=255)
                                             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                         >  2:4460127/1‑101 (MQ=255)
                                             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                         >  1:4215093/1‑101 (MQ=255)
                                             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                         >  1:3281585/1‑101 (MQ=255)
                                              cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                        <  1:3315274/101‑1 (MQ=255)
                                               aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                       >  2:3958957/1‑101 (MQ=255)
                                                 cggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggg                                                     >  1:642019/1‑101 (MQ=255)
                                                  ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                    <  1:1333073/101‑1 (MQ=255)
                                                    cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                  <  1:3767699/101‑1 (MQ=255)
                                                       cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:3651588/1‑101 (MQ=255)
                                                       cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:4390516/1‑101 (MQ=255)
                                                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:743442/1‑101 (MQ=255)
                                                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  2:1094444/101‑1 (MQ=255)
                                                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  2:1631136/101‑1 (MQ=255)
                                                           tCGCCATGACGCTCGCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:4100473/101‑1 (MQ=255)
                                                            cGCCATGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:2262771/1‑101 (MQ=255)
                                                             gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGATGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  2:403115/1‑101 (MQ=255)
                                                                aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  1:4238265/101‑1 (MQ=255)
                                                                 cgatcttCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     <  1:2559405/95‑1 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                   <  1:2423656/101‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                  <  2:906001/101‑1 (MQ=255)
                                                                     gccccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                 <  2:3459457/98‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                 <  1:2232356/101‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                 <  2:2719444/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                <  1:238863/101‑1 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                               >  1:3951679/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGTGGCGGGGATCCGAACCTTATggac                             >  2:1993252/1‑99 (MQ=37)
                                                                         cccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                             <  2:2518669/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                            <  2:3771322/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                            <  1:1705981/101‑1 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                           <  1:4350799/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                          >  2:591749/1‑101 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                         <  1:2769066/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                        <  1:1679884/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                       >  1:4307228/1‑101 (MQ=255)
                                                                                gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                      >  1:4641623/1‑101 (MQ=255)
                                                                                gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                      <  1:4177460/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                     <  1:3150732/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                     <  1:1005664/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggag                   >  1:3521407/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                >  1:4032460/1‑101 (MQ=255)
                                                                                       tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta               <  2:2529722/101‑1 (MQ=255)
                                                                                        ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc              <  1:1940615/101‑1 (MQ=255)
                                                                                        ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc              <  1:1169439/101‑1 (MQ=255)
                                                                                            cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          >  1:379553/1‑101 (MQ=255)
                                                                                            cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          <  2:3281384/101‑1 (MQ=255)
                                                                                            cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          <  1:595692/101‑1 (MQ=255)
                                                                                             gAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCaac         <  2:2368396/101‑3 (MQ=255)
                                                                                             gAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc         >  2:258676/1‑101 (MQ=255)
                                                                                               aaCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt       <  2:1694238/101‑1 (MQ=255)
                                                                                                 cccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCa     >  1:2661210/1‑101 (MQ=255)
                                                                                                    ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa  <  1:2367169/101‑1 (MQ=255)
                                                                                                    ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa  <  2:641955/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397770‑1397970

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG