Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,412,637 T→C 100% S398G (AGC→GGC)  RCAP_rcc01318 ← outer membrane autotransporter barrel domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,412,6370T→C100.0% 644.8 181S398G (AGC→GGC) RCAP_rcc01318outer membrane autotransporter barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (99/82):  ref base (0/0):  total (99/82)

Only 100 of 189 total aligned reads displayed.

CTGATCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCTCGCGACTTGGGCGTCAACATGGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACA  >  NC_014034/1412539‑1412734
                                                                                                  |                                                                                                 
cTGATCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcg                                                                                                 >  2:3277497/1‑101 (MQ=255)
  gATCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcgcg                                                                                               <  1:195489/101‑1 (MQ=255)
   aTCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGa                                                                                              >  2:4157074/1‑101 (MQ=255)
    tCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGAc                                                                                             >  2:1866668/1‑101 (MQ=255)
            cgtTCGCACCTCCGTCCCGTTCACCCCGAAACTCGTGCCGGCAAGAGACAGGCCGTCTCCCGCGGTCTAGGTGGTGCCCACCGCCCCCGCGACTTGGGCGt                                                                                     <  2:4488899/101‑1 (MQ=14)
                cgcACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAAc                                                                                 <  1:1538601/101‑1 (MQ=255)
                 gcACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACa                                                                                <  1:4295234/101‑1 (MQ=255)
                 gcACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACa                                                                                <  2:3631341/101‑1 (MQ=255)
                  caccacCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAt                                                                               <  2:2305575/101‑1 (MQ=255)
                  caccacCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAt                                                                               >  2:1316544/1‑101 (MQ=255)
                   gcccccGTGCCGTTCACTGCGAAAGTGGGGGCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAta                                                                              <  2:2246156/97‑2 (MQ=14)
                   accaccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAta                                                                              >  1:363678/1‑100 (MQ=255)
                   accaccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAta                                                                              >  2:4166643/1‑100 (MQ=255)
                   accaccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAta                                                                              <  1:851341/101‑2 (MQ=255)
                   accaccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAta                                                                              >  2:1262517/1‑100 (MQ=255)
                    ccaccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACAtag                                                                             <  1:1033775/101‑3 (MQ=255)
                     caccGTACCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCNCCGCGCCCGCGACTTGGGCGTCAACATagg                                                                            >  1:2269655/1‑101 (MQ=255)
                       ccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                                                          >  1:96972/1‑101 (MQ=255)
                        cGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCt                                                                         >  2:1315013/1‑101 (MQ=255)
                         gttccGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                                                        <  1:3824555/98‑1 (MQ=255)
                         gTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                                                        >  1:4382034/1‑101 (MQ=255)
                          tGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                                                       <  2:2607234/101‑1 (MQ=255)
                          tGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                                                       >  2:836313/1‑101 (MQ=255)
                          gtccGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                                                       <  2:4548177/99‑1 (MQ=255)
                           gCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCg                                                                      <  1:4247662/101‑1 (MQ=255)
                            ccGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGa                                                                     >  1:785394/1‑101 (MQ=255)
                             cGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAg                                                                    >  1:176865/1‑101 (MQ=255)
                              gTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAgg                                                                   >  2:1144163/1‑101 (MQ=16)
                              gTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAgg                                                                   >  1:4112402/1‑101 (MQ=19)
                                tCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                                                                 >  1:1754871/1‑101 (MQ=12)
                                tCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                                                                 >  1:3290707/1‑101 (MQ=12)
                                     gCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTg                                                            >  2:4564970/1‑101 (MQ=2)
                                      cGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTgg                                                           >  2:1354787/1‑101 (MQ=2)
                                        aaaGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGcc                                                         >  1:4586731/1‑101 (MQ=2)
                                        aaaGTGGCGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCCCGCCGGCGAGTTGGGGGTCAGCATCGGCCTTGGAGGCCACCTGGcc                                                         >  2:4678662/1‑101 (MQ=11)
                                                gCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCg                                                 >  1:3506037/1‑101 (MQ=9)
                                                    gCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgg                                             >  2:2145475/1‑101 (MQ=255)
                                                      aaGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGCGGCCACCTGGCCCGCAAGCGTCgggg                                           >  1:1483684/1‑101 (MQ=255)
                                                      aaGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgggg                                           >  2:922325/1‑101 (MQ=255)
                                                      aaGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgggg                                           >  2:4751034/1‑101 (MQ=255)
                                                       agagCCAGGCGGTCTCCCGCCGCATAGGTGGTTCCCTCCGCACCCGCGACCTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGc                                          <  2:3178478/101‑1 (MQ=14)
                                                       agagACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGc                                          >  1:560529/1‑101 (MQ=255)
                                                         agaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcg                                        <  2:4142920/101‑1 (MQ=255)
                                                         agaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcg                                        >  2:3350385/1‑101 (MQ=255)
                                                         agaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcg                                        >  1:3796627/1‑101 (MQ=255)
                                                           aCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcgcg                                      <  1:4664154/101‑1 (MQ=255)
                                                           aCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcgcg                                      <  1:1466549/101‑1 (MQ=255)
                                                           aCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcgcg                                      >  2:4557123/1‑101 (MQ=255)
                                                           aCAGGCCGTCTCCCGCGGCATAGGTGGTGCACACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcgcg                                      >  1:4222016/1‑101 (MQ=255)
                                                               gCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCg                                  <  1:1184371/101‑1 (MQ=255)
                                                                ccGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCgg                                 <  2:3754439/101‑1 (MQ=255)
                                                                 cgcctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCggg                                <  1:3248726/98‑1 (MQ=255)
                                                                  gTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGa                               >  2:1114392/1‑101 (MQ=255)
                                                                  gTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGa                               >  1:1133734/1‑101 (MQ=255)
                                                                  gTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGa                               >  2:1940941/1‑101 (MQ=255)
                                                                   tctcCCGCGGCATAGGTGGTGCCCACCGCTCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAc                              <  1:3900336/101‑1 (MQ=255)
                                                                    ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                             >  2:4348469/1‑101 (MQ=255)
                                                                    ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                             <  1:4750761/101‑1 (MQ=255)
                                                                    ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                             <  1:2217685/101‑1 (MQ=255)
                                                                    ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                             >  1:243533/1‑101 (MQ=255)
                                                                     tcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCg                            >  2:4309522/1‑101 (MQ=255)
                                                                      cccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGGCGCGGTCGGGGCCgc                           >  2:3386646/1‑100 (MQ=14)
                                                                      cccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGt                           >  2:2987179/1‑101 (MQ=255)
                                                                      cccGCGGCATAGGTGGTGCCCACCGCGCCCACGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGt                           >  1:4221027/1‑101 (MQ=255)
                                                                       ccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTc                          <  2:2716854/101‑1 (MQ=255)
                                                                       ccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTc                          >  1:2796518/1‑101 (MQ=255)
                                                                        cgcgGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCg                         <  1:708880/101‑1 (MQ=255)
                                                                          cgGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGc                       <  2:775232/101‑1 (MQ=255)
                                                                           ggCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCa                      >  1:2170550/1‑101 (MQ=255)
                                                                           ggCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCa                      <  2:2107675/101‑1 (MQ=255)
                                                                            gCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAg                     <  1:298691/101‑1 (MQ=255)
                                                                               tAGGTGGTGCCCCCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGa                  <  2:4221769/101‑1 (MQ=255)
                                                                                aGGTGGTGCCCACCGCGCCCGCGACTTGTGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                 >  1:4307570/1‑101 (MQ=255)
                                                                                aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAgggcc                 >  2:3642417/1‑99 (MQ=255)
                                                                                aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                 >  2:4633858/1‑101 (MQ=255)
                                                                                aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                 <  1:1256052/101‑1 (MQ=255)
                                                                                aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                 <  2:1457412/101‑1 (MQ=255)
                                                                                 ggtggtGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACg                >  2:542274/1‑101 (MQ=255)
                                                                                 ggtggtGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACg                >  1:3380405/1‑101 (MQ=255)
                                                                                   tggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgag              <  1:1055776/101‑1 (MQ=255)
                                                                                   tggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgag              >  2:269808/1‑101 (MQ=255)
                                                                                   tggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgag              >  1:286327/1‑101 (MQ=255)
                                                                                    ggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgaga             <  1:3959368/101‑1 (MQ=255)
                                                                                    ggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgaga             >  2:1167627/1‑101 (MQ=255)
                                                                                     gtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGaa            <  1:1866780/101‑1 (MQ=255)
                                                                                      tgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAg           >  1:3759693/1‑101 (MQ=255)
                                                                                       gCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGt          >  1:3058469/1‑101 (MQ=255)
                                                                                        cccACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtt         <  2:2661416/101‑1 (MQ=255)
                                                                                        cccACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtt         >  2:1182431/1‑101 (MQ=255)
                                                                                        cccACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtt         >  1:3480331/1‑101 (MQ=255)
                                                                                          cACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtttt       <  2:3290505/101‑1 (MQ=255)
                                                                                          cACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtttt       <  2:3810943/101‑1 (MQ=255)
                                                                                           aCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGttttt      >  2:866334/1‑101 (MQ=255)
                                                                                           aCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTAGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGgtttt      >  2:4103565/1‑101 (MQ=21)
                                                                                            ccGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTc     <  2:1129305/101‑1 (MQ=255)
                                                                                             cgcgCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTca    <  2:286293/101‑1 (MQ=255)
                                                                                             cgcgCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTca    >  1:4720831/1‑101 (MQ=255)
                                                                                             cgcgCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTca    <  2:1695743/101‑1 (MQ=255)
                                                                                              gcgcCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTcac   <  1:3416345/101‑1 (MQ=255)
                                                                                               cgcCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTcaca  <  2:785321/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTGATCGCCCGTCGTGCGCACCACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCTCGCGACTTGGGCGTCAACATGGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACA  >  NC_014034/1412539‑1412734

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG