Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,385 T→G 11.4% intergenic (‑164/‑794) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3850T→G11.4% 77.8 ‑0.8 ‑0.1288intergenic (‑164/‑794)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (16/17):  ref base (106/149):  total (122/166)
Fisher's exact test strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.46e-01

Only 100 of 312 total aligned reads displayed.

GGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGC  >  NC_014034/1434285‑1434485
                                                                                                      |                                                                                                        
ggTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtct                                                                                                          >  2:1488859/1‑101 (MQ=255)
 gTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtctc                                                                                                         <  2:3823216/101‑1 (MQ=255)
  tataTGCACCGCTTGCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                        <  2:2765347/101‑1 (MQ=255)
  tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                        <  2:854622/101‑1 (MQ=255)
  tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                        >  1:2869727/1‑101 (MQ=255)
     atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                    >  1:4300050/1‑101 (MQ=255)
     atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                    >  2:2469780/1‑101 (MQ=255)
        cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACAGCTGATCCTGCTGGGTCGCTGCCCCCGCGGGGGCGTGGATCGAAAC..CTCTCCG.CTCTg                                                                                                 >  1:4062697/1‑101 (MQ=255)
          ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ct                                                                                              <  1:2317018/101‑1 (MQ=255)
           cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ctt                                                                                             <  1:974268/101‑1 (MQ=255)
            gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                            <  1:4154557/101‑1 (MQ=255)
             cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCa                                                                                           <  2:368655/101‑1 (MQ=255)
              ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                          >  2:4442955/1‑101 (MQ=255)
              ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                          <  2:2839622/101‑1 (MQ=255)
              ttACCGTGGATAGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                          >  2:3158803/1‑101 (MQ=255)
                  cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                     <  1:3816984/101‑1 (MQ=255)
                  cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                     <  1:4380183/101‑1 (MQ=255)
                   gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                    <  2:4720690/101‑1 (MQ=255)
                    tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.c                                                                                  >  1:1247494/1‑101 (MQ=255)
                      gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                                >  2:2082344/1‑101 (MQ=255)
                        tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCa                                                                              <  2:1150712/101‑1 (MQ=255)
                          gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                            >  2:3053585/1‑101 (MQ=255)
                          gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                            >  1:2930792/1‑101 (MQ=255)
                          gAAACAGGCTGCTTTGGGCGGAACACCTGATACTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                            >  1:4206891/1‑101 (MQ=255)
                           aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                           >  1:3439144/1‑101 (MQ=255)
                           aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                           >  2:1211739/1‑101 (MQ=255)
                              cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                        <  2:1972907/101‑1 (MQ=255)
                               aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGt                                                                       >  1:4465316/1‑101 (MQ=255)
                                ggttgctTTGGGCGGAACACCTTATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTc                                                                      <  1:3739267/98‑1 (MQ=255)
                                ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTc                                                                      <  1:3139922/101‑1 (MQ=255)
                                  ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGc                                                                    <  1:2497563/101‑1 (MQ=255)
                                   tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCt                                                                   <  2:1605897/101‑1 (MQ=255)
                                     ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGGCGCTcc                                                                 >  1:1580015/1‑101 (MQ=255)
                                        tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                              >  2:1252571/1‑101 (MQ=255)
                                        tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                              >  1:491401/1‑101 (MQ=255)
                                        tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                              >  2:4659553/1‑101 (MQ=255)
                                         gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                             <  2:4217677/101‑1 (MQ=255)
                                         gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                             <  2:1712119/101‑1 (MQ=255)
                                         gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                             >  2:3960243/1‑101 (MQ=255)
                                          ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCcg                                                            <  2:670519/101‑1 (MQ=255)
                                              gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggg                                                        <  1:1141558/101‑1 (MQ=255)
                                              gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggg                                                        <  1:1268550/101‑1 (MQ=255)
                                                acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggggg                                                      <  1:1725952/101‑1 (MQ=255)
                                                acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggggg                                                      <  1:2665793/101‑1 (MQ=255)
                                                 cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                     <  1:3080321/101‑1 (MQ=255)
                                                    cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                  >  1:1532619/1‑101 (MQ=255)
                                                     tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCCCGGGGGCGGgg                                                 >  1:3666944/1‑101 (MQ=255)
                                                      gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggttcgtctg                                                <  2:1093965/101‑9 (MQ=255)
                                                        tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:2630357/101‑1 (MQ=255)
                                                        tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGGGGGTc                                              >  1:3974993/1‑101 (MQ=255)
                                                         ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:551404/101‑1 (MQ=255)
                                                         ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             >  1:1837975/1‑101 (MQ=255)
                                                          ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  2:188635/94‑1 (MQ=2)
                                                           tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  1:3250021/1‑101 (MQ=255)
                                                           gctatcgTCGATCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:1239156/95‑1 (MQ=2)
                                                             tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            >  1:386032/5‑101 (MQ=2)
                                                             tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            >  1:4712853/5‑101 (MQ=2)
                                                             ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                         <  2:2238436/101‑1 (MQ=255)
                                                              tgGGTCGCCCCCCCCGCGGGGGCGTGGATTGGAAC..GTCCCCG.GTCTG.CTTCCCG.GCTG.GGTCAACCTTGTCGCCCCCCCCCCGGGGGCGTGGATCGAAaca                                        <  2:3887956/101‑1 (MQ=25)
                                                              ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCGCTGACACCGCCAACAAA.TATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:1604566/98‑1 (MQ=2)
                                                              atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  2:2039956/4‑101 (MQ=2)
                                                               tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  2:766216/3‑101 (MQ=2)
                                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                      <  2:4062455/101‑1 (MQ=255)
                                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                      >  2:3998512/1‑101 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                       <  2:539958/101‑3 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACggc                                    >  2:1819929/1‑100 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                      <  2:2379430/101‑4 (MQ=2)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                  <  1:573012/101‑1 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                    <  2:1417048/101‑6 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                 <  2:3600341/101‑1 (MQ=255)
                                                                      cccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.ATTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                <  1:3320824/100‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                  >  1:3943596/1‑94 (MQ=2)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                               <  1:2037621/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                 <  1:4523520/101‑9 (MQ=2)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                              <  1:320937/101‑1 (MQ=255)
                                                                         cgccGCGGGGGCGGGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                             <  2:1379618/99‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                             <  2:2930616/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                             <  1:2252387/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                           <  2:4623201/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                          <  2:3438937/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                         <  2:1775532/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                       >  2:2136676/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                     >  1:2439875/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                    >  2:3386038/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                  >  1:2895482/1‑101 (MQ=255)
                                                                                     gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                 >  1:3478624/1‑101 (MQ=255)
                                                                                     gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                 >  1:1757774/1‑101 (MQ=255)
                                                                                      tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCCAGGGGCa                >  2:1182921/1‑101 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg               >  2:2968201/1‑101 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg               >  1:788125/1‑101 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg               >  1:2546965/1‑101 (MQ=255)
                                                                                        gATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg              >  1:2002631/1‑101 (MQ=255)
                                                                                           cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt           <  2:1639873/101‑1 (MQ=255)
                                                                                            gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt          <  1:1488960/101‑1 (MQ=255)
                                                                                             aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg         >  2:4537277/1‑101 (MQ=255)
                                                                                             aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg         <  1:4659809/101‑1 (MQ=255)
                                                                                              aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc        <  2:2263216/101‑1 (MQ=255)
                                                                                              aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc        <  1:1005468/101‑1 (MQ=255)
                                                                                               aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa       <  1:2143948/101‑1 (MQ=255)
                                                                                                c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg      <  2:1532518/101‑1 (MQ=255)
                                                                                                      |                                                                                                        
GGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGC  >  NC_014034/1434285‑1434485

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG