Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,387 C→A 11.7% intergenic (‑166/‑792) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3870C→A11.7% 80.9 ‑0.8 ‑0.0286intergenic (‑166/‑792)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (16/17):  ref base (103/145):  total (120/166)
Fisher's exact test strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.34e-01

Only 100 of 308 total aligned reads displayed.

TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487
                                                                                                      |                                                                                                          
tataTGCACCGCTTGCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                            <  2:2765347/101‑1 (MQ=255)
tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                            >  1:4547410/1‑101 (MQ=255)
tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                            >  1:4426198/1‑101 (MQ=255)
tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                            <  2:854622/101‑1 (MQ=255)
   atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                        >  1:4166474/1‑101 (MQ=255)
   atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                        >  2:2469780/1‑101 (MQ=255)
     gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctct                                                                                                      <  1:2610646/101‑1 (MQ=255)
     gCACCGCTTACCGTGGATCGAAACAGGCTGCTTAGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCAG.ctct                                                                                                      <  1:4085513/101‑1 (MQ=255)
       aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGGCGCTCGCAAC..GTCTCCG.CTCTG.c                                                                                                   >  1:3942882/1‑101 (MQ=255)
        ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ct                                                                                                  <  1:2317018/101‑1 (MQ=255)
         cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ctt                                                                                                 <  1:974268/101‑1 (MQ=255)
          gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                                <  1:373277/101‑1 (MQ=255)
            ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                              <  2:2839622/101‑1 (MQ=255)
            ttACCGTGGATAGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                              >  2:3158803/1‑101 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                         >  1:1809786/1‑101 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                         >  1:4485369/1‑101 (MQ=255)
                 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                        <  2:4720690/101‑1 (MQ=255)
                  tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.c                                                                                      >  1:1247494/1‑101 (MQ=255)
                    gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                                    >  2:2082344/1‑101 (MQ=255)
                      tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCa                                                                                  <  2:1150712/101‑1 (MQ=255)
                        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                >  2:3053585/1‑101 (MQ=255)
                        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                >  1:2806947/1‑101 (MQ=255)
                         aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                               >  2:1211739/1‑101 (MQ=255)
                          aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                              <  1:3238346/101‑1 (MQ=255)
                            cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..g                                                                          <  2:1972907/101‑1 (MQ=255)
                               gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCg                                                                       >  2:1725847/1‑101 (MQ=255)
                               gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCg                                                                       >  2:1470741/1‑101 (MQ=255)
                                 tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCt                                                                     <  2:1605897/101‑1 (MQ=255)
                                 tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCt                                                                     >  1:251791/1‑101 (MQ=255)
                                  gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTc                                                                    <  1:3461029/101‑1 (MQ=255)
                                   ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GGCGCTcc                                                                   >  1:1580015/1‑101 (MQ=255)
                                      tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                >  2:4659553/1‑101 (MQ=255)
                                      tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                >  2:1252571/1‑101 (MQ=255)
                                      tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                >  1:2386248/1‑101 (MQ=255)
                                       gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                               >  2:3960243/1‑101 (MQ=255)
                                        ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcg                                                              <  2:670519/101‑1 (MQ=255)
                                          cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgcg                                                            <  2:4394816/101‑1 (MQ=255)
                                            gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggg                                                          <  1:1141558/101‑1 (MQ=255)
                                            gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggg                                                          <  1:1268550/101‑1 (MQ=255)
                                             aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTGAACCTT..GTCGCTCCCCCCGCgggg                                                         <  1:2664053/101‑1 (MQ=255)
                                               cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                       <  2:4130055/101‑1 (MQ=255)
                                                  cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                    >  1:1532619/1‑101 (MQ=255)
                                                   tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                   <  1:2469923/101‑1 (MQ=255)
                                                    gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgggttcgtctg                                                  <  2:1093965/101‑9 (MQ=255)
                                                         gctatcgTCGATCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:1239156/95‑1 (MQ=2)
                                                           tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  1:1757434/97‑1 (MQ=2)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:3782795/101‑1 (MQ=255)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:2238436/101‑1 (MQ=255)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  1:3424868/101‑1 (MQ=255)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           >  1:4693355/1‑101 (MQ=255)
                                                            tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                          >  2:1839549/1‑101 (MQ=255)
                                                            tgGGTCGCCCCCCCCGCGGGGGCGTGGATTGGAAC..GTCCCCG.GTCTG.CTTCCCG.GCTG.GGTCAACCTT..GTCGCCCCCCCCCCGGGGGCGTGGATCGAAaca                                          <  2:3887956/101‑1 (MQ=25)
                                                            ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:1604566/98‑1 (MQ=2)
                                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  2:2039956/4‑101 (MQ=2)
                                                             tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            >  2:766216/3‑101 (MQ=2)
                                                             gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         <  1:4261608/101‑1 (MQ=255)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                         <  2:539958/101‑3 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                        <  2:2379430/101‑4 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                     >  1:384150/1‑101 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                      <  2:1417048/101‑6 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                      >  1:1725575/1‑96 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                   <  2:4465066/101‑1 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                   <  2:3600341/101‑1 (MQ=255)
                                                                    tCCGCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAACGGATTGc                                  >  2:4022796/1‑101 (MQ=255)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                  >  1:289408/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                    >  1:3287806/1‑94 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                 <  1:2037621/101‑1 (MQ=255)
                                                                       cgccGCGGGGGCGGGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                               <  2:1379618/99‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAAC..TTGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                >  1:3138297/1‑94 (MQ=37)
                                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                               <  2:2930616/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                               <  1:2252387/101‑1 (MQ=255)
                                                                         ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                             >  1:4741220/1‑101 (MQ=255)
                                                                         ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                             <  2:4623201/101‑1 (MQ=255)
                                                                          cgcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                            <  2:3438937/101‑1 (MQ=255)
                                                                           gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                           <  2:1888606/101‑1 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                          <  1:1937096/101‑1 (MQ=255)
                                                                             gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                         >  2:2136676/1‑101 (MQ=255)
                                                                                ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                      >  2:3386038/1‑101 (MQ=255)
                                                                                 gCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                     <  1:3772543/101‑1 (MQ=255)
                                                                                    tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                  >  1:538889/1‑101 (MQ=255)
                                                                                    tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCCAGGGGCa                  >  2:1182921/1‑101 (MQ=255)
                                                                                     ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGGGGGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                 >  1:568543/1‑101 (MQ=255)
                                                                                     ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                 >  2:2968201/1‑101 (MQ=255)
                                                                                     ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                 <  2:1789639/101‑1 (MQ=255)
                                                                                        tCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt              >  1:3512429/1‑101 (MQ=255)
                                                                                         cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTGCCTCCACCCGCGGGGGCGCGGATCGAAACACGGATTGCATGGCGCCGGGGGACAGGCtt             >  1:798236/1‑101 (MQ=21)
                                                                                         cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt             <  2:1639873/101‑1 (MQ=255)
                                                                                          gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            <  1:1488960/101‑1 (MQ=255)
                                                                                           aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg           >  2:4537277/1‑101 (MQ=255)
                                                                                            aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc          <  2:2263216/101‑1 (MQ=255)
                                                                                            aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc          <  1:1005468/101‑1 (MQ=255)
                                                                                            aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc          <  1:4306900/101‑1 (MQ=255)
                                                                                             aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa         <  1:2143948/101‑1 (MQ=255)
                                                                                              c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        <  2:1532518/101‑1 (MQ=255)
                                                                                              c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        >  1:3177075/1‑101 (MQ=255)
                                                                                              c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        <  1:3077935/101‑1 (MQ=255)
                                                                                              c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        >  1:2924230/1‑101 (MQ=255)
                                                                                                  tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc      <  1:3960485/101‑1 (MQ=255)
                                                                                                   ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg     <  1:620329/101‑1 (MQ=255)
                                                                                                    tcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc    >  2:3680768/1‑101 (MQ=255)
                                                                                                      |                                                                                                          
TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG