Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,389 C→A 13.1% intergenic (‑168/‑790) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3890C→A13.1% 78.8 ‑0.5 ‑0.0274intergenic (‑168/‑790)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (17/19):  ref base (99/138):  total (116/158)
Fisher's exact test strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 300 total aligned reads displayed.

TATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCC  >  NC_014034/1434289‑1434489
                                                                                                       |                                                                                                         
tatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.c                                                                                                           <  2:3832348/101‑1 (MQ=255)
  tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctc                                                                                                         <  1:2246039/101‑1 (MQ=255)
   gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctct                                                                                                        <  2:727979/101‑1 (MQ=255)
    cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTg                                                                                                       <  2:2871394/101‑1 (MQ=255)
    cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTg                                                                                                       <  2:138056/101‑1 (MQ=255)
       cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ctt                                                                                                   <  2:2359786/101‑1 (MQ=255)
       cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ctt                                                                                                   <  1:974268/101‑1 (MQ=255)
       cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.Ctt                                                                                                   <  2:3225459/101‑1 (MQ=255)
        gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                                  >  1:413307/1‑101 (MQ=255)
         cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCa                                                                                                 >  2:2035877/1‑101 (MQ=255)
            aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACg.g                                                                                             >  1:122858/1‑101 (MQ=255)
             ccGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAAC..GTCTCCG.CTCTG.CTTCACG.Gc                                                                                            >  1:3986812/1‑101 (MQ=255)
              cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                           <  1:3816984/101‑1 (MQ=255)
              cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                           >  1:1809786/1‑101 (MQ=255)
              cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                           <  1:3133525/101‑1 (MQ=255)
              cGTGGATCGAAACAGGCTGCTTTGGGCGGAACAACTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                           >  2:1222957/1‑101 (MQ=255)
               gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                          <  1:2306413/101‑1 (MQ=255)
                  gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                                      >  2:2082344/1‑101 (MQ=255)
                   aTCGGAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTc                                                                                     <  2:1424211/101‑1 (MQ=255)
                      gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                  <  1:569680/101‑1 (MQ=255)
                      gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                  >  2:523855/1‑101 (MQ=255)
                        aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                                <  2:2790899/101‑1 (MQ=255)
                          cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..g                                                                            <  2:1972907/101‑1 (MQ=255)
                           aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..Gt                                                                           >  1:4465316/1‑101 (MQ=255)
                           aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCACCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GGTG.CGGCAACCTT..gc                                                                           >  2:2423073/1‑100 (MQ=255)
                             gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCg                                                                         >  2:1725847/1‑101 (MQ=255)
                              ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGc                                                                        <  1:2497563/101‑1 (MQ=255)
                              ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGc                                                                        <  2:2689251/101‑1 (MQ=255)
                                 ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GGCGCTcc                                                                     >  1:1580015/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTNAACCTT..GTCGCTccccc                                                                  >  2:1591396/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                  >  2:1252571/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                  >  1:2132620/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                  >  1:491401/1‑101 (MQ=255)
                                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                 <  2:866143/101‑1 (MQ=255)
                                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                 <  1:143759/101‑1 (MQ=255)
                                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                 <  1:2035998/101‑1 (MQ=255)
                                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                 <  1:2460746/101‑1 (MQ=255)
                                       gCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgc                                                               <  1:3481027/101‑1 (MQ=255)
                                        cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgcg                                                              <  2:4394816/101‑1 (MQ=255)
                                          gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggg                                                            <  1:1141558/101‑1 (MQ=255)
                                           atctccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgggg                                                           <  2:1491122/97‑1 (MQ=255)
                                            acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggggg                                                          <  1:2665793/101‑1 (MQ=255)
                                             cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                         >  1:1264053/1‑101 (MQ=255)
                                             cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                         <  1:4179143/101‑1 (MQ=255)
                                                cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:1532619/1‑101 (MQ=255)
                                                  gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgggttcgtctg                                                    <  2:1093965/101‑9 (MQ=255)
                                                   gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                      <  2:2951255/91‑1 (MQ=2)
                                                   aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgggt                                                   >  2:3742947/1‑99 (MQ=255)
                                                   aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:620265/1‑101 (MQ=255)
                                                   aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:124718/101‑1 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:2630357/101‑1 (MQ=255)
                                                     ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:3442562/101‑1 (MQ=255)
                                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCTAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:2372841/101‑1 (MQ=255)
                                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               >  2:4054996/1‑101 (MQ=255)
                                                       gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  2:4640334/7‑101 (MQ=2)
                                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:3680870/96‑1 (MQ=2)
                                                         tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:1757434/97‑1 (MQ=2)
                                                         tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                >  1:4712853/5‑101 (MQ=2)
                                                         tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                >  1:3250685/5‑101 (MQ=2)
                                                          tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                            >  2:1839549/1‑101 (MQ=255)
                                                          ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:1604566/98‑1 (MQ=2)
                                                          atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               >  1:43115/4‑101 (MQ=2)
                                                           tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  2:3130573/99‑1 (MQ=2)
                                                           gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                           <  1:4261608/101‑1 (MQ=255)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                          >  2:366209/1‑101 (MQ=255)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                          >  2:1156522/1‑101 (MQ=255)
                                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                             >  2:3387093/2‑101 (MQ=2)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                            >  2:3917046/1‑100 (MQ=2)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                            >  1:2891802/1‑100 (MQ=2)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                            >  2:2202824/1‑100 (MQ=2)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                        >  2:1195409/1‑101 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                      <  1:3955314/101‑1 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                        >  1:1725575/1‑96 (MQ=2)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                     <  2:1616399/101‑1 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                     <  2:4465066/101‑1 (MQ=255)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                    >  2:3031940/1‑101 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                   <  1:294543/101‑1 (MQ=255)
                                                                     ccccGCGGGGGCGTGGATCGAAAC..TTGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                  <  1:4520794/101‑8 (MQ=37)
                                                                       ccGCGGGAGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                               <  2:3583990/101‑1 (MQ=255)
                                                                         gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                             >  1:3866284/1‑101 (MQ=255)
                                                                         gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                             <  1:3335747/101‑1 (MQ=255)
                                                                         gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                             <  2:1888606/101‑1 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                            <  1:1937096/101‑1 (MQ=255)
                                                                           gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCGCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                           <  1:37542/101‑1 (MQ=255)
                                                                                   tgATTGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                   <  2:2255383/100‑1 (MQ=255)
                                                                                   ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                   >  1:2546965/1‑101 (MQ=255)
                                                                                   ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                   <  2:3986570/101‑1 (MQ=255)
                                                                                   ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                   <  2:1789639/101‑1 (MQ=255)
                                                                                    gATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                  <  2:168677/101‑1 (MQ=255)
                                                                                       cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTGCCTCCACCCGCGGGGGCGCGGATCGAAACACGGATTGCATGGCGCCGGGGGACAGGCtt               >  1:798236/1‑101 (MQ=21)
                                                                                       cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt               <  2:4166228/101‑1 (MQ=255)
                                                                                       cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt               >  1:690576/1‑101 (MQ=255)
                                                                                        gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt              >  1:3221096/1‑101 (MQ=255)
                                                                                        gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt              <  1:3159003/101‑1 (MQ=255)
                                                                                         aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg             >  2:4537277/1‑101 (MQ=255)
                                                                                         aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg             <  1:4659809/101‑1 (MQ=255)
                                                                                          aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc            <  1:1005468/101‑1 (MQ=255)
                                                                                            c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg          >  1:54977/1‑101 (MQ=255)
                                                                                            c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg          >  1:2924230/1‑101 (MQ=255)
                                                                                                 ctcCG.CTCTG.CTTCCCT.GCTC.CGTCAACCGT..GTCGGAGCCCGCGCGGGGGCGTGGATCGTGACACAGATTGCATGGCGCCGTGTGACAGGCTTTGCAGTcc       <  2:4676152/101‑2 (MQ=12)
                                                                                                       |                                                                                                         
TATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCC  >  NC_014034/1434289‑1434489

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG