Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,933 G→C 100% intergenic (+203/‑144) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9330G→C100.0% 449.1 122intergenic (+203/‑144)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (43/79):  ref base (0/0):  total (43/79)

Only 100 of 142 total aligned reads displayed.

GCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCCTCTTGGGC  >  NC_014034/1607834‑1608029
                                                                                                   |                                                                                                  
gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                                >  1:10013/1‑101 (MQ=255)
gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                                >  2:4302263/1‑101 (MQ=255)
 ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCt                                                                                               <  1:1974516/101‑1 (MQ=255)
   aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCCGt                                                                                             >  1:4616334/1‑101 (MQ=255)
   aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAAAGCGGGC.CGACCGGGGC.GACCGCCGCCTgc                                                                                             >  1:4365869/1‑100 (MQ=255)
    cGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                                            <  2:2343067/101‑1 (MQ=255)
      cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                                                          <  2:2325916/101‑2 (MQ=255)
      cccGCATTGGGCCCGCCGCGCCCCAGAACGCGCGCCCAGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                                                          <  1:4008234/101‑2 (MQ=255)
       ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                                         <  1:1854767/101‑3 (MQ=255)
        cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                                        <  2:4614988/101‑4 (MQ=255)
           cttGTGCCCGCGGCGCCCCAGAACGGCCGCCCCGGCCCCTCAAGGATGGCGGC.GCCCCGGGAAGGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                                                    <  2:2209586/100‑1 (MQ=255)
           aTTGTGGCTGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCCCCTGGCTCCCgcc                                                                                     >  2:2957928/1‑99 (MQ=255)
           aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                                                    >  2:1497527/1‑101 (MQ=255)
             tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCtt                                                                                  >  1:4296006/1‑101 (MQ=255)
             tggggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                                  <  1:2893986/98‑1 (MQ=255)
              gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                                                 <  1:1203393/101‑1 (MQ=255)
                 gccgccGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTcc                                                                              <  1:1332547/101‑1 (MQ=255)
                  ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGTCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                             <  1:767612/101‑1 (MQ=255)
                  ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                             <  2:3474024/101‑1 (MQ=255)
                  ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCTTTTccc                                                                             >  1:626935/1‑101 (MQ=255)
                   cgccgcGCCCCAGACCGGGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GCGAATGCGGGC.AGACCGGG.CGGACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                            <  2:4748810/101‑1 (MQ=255)
                   cgccgcGCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                            <  2:944091/101‑1 (MQ=255)
                   cgccgcGCCCCAGAACGCGCGCCCCGGCCGCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                            <  2:4365622/101‑1 (MQ=255)
                    gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                           <  2:285604/101‑1 (MQ=255)
                     ccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGc                                                                          >  1:266805/1‑101 (MQ=255)
                      cgcgCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCg                                                                         <  2:4611151/101‑1 (MQ=255)
                      cgcgCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGGCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCg                                                                         <  2:542296/101‑1 (MQ=255)
                       gcgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCgg                                                                        <  2:428017/101‑1 (MQ=255)
                       gcgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.CACCGCCGCCCGTC.TCCGACCTTTTCCCAGCgg                                                                        >  2:394846/1‑101 (MQ=255)
                         gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                                      <  1:3214110/101‑1 (MQ=255)
                         gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGACC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCgggt                                                                      >  1:3305676/1‑99 (MQ=255)
                          ccccAGAGCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACGGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                                                     <  2:1344264/101‑1 (MQ=255)
                           cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                                                    <  2:3920880/101‑1 (MQ=255)
                           cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                                                    <  2:610368/101‑1 (MQ=255)
                           cccAGAACGCGCGCCCCAGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                                                    >  1:1720973/1‑101 (MQ=255)
                            ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  2:3691872/101‑1 (MQ=255)
                            ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  2:2339750/101‑1 (MQ=255)
                            ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   >  2:1985160/1‑101 (MQ=255)
                              aGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGG.CCGGGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                 <  1:355772/101‑1 (MQ=255)
                               gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                <  1:2977463/101‑1 (MQ=255)
                                aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGa                                                               <  1:1216371/101‑1 (MQ=255)
                                aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGa                                                               <  1:478409/101‑1 (MQ=255)
                                  cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                             <  2:3760066/101‑1 (MQ=255)
                                    cgcgcCCCGGCCCCTCAAGGATGGCGGGGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTACCCGCGGATCCCGCGACggg                                                           >  2:2458509/1‑101 (MQ=255)
                                    cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCTTTTCCCAGCGGATCCCCCGCCggg                                                           >  2:1406761/1‑101 (MQ=255)
                                       gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                                        <  2:1219930/101‑1 (MQ=255)
                                        ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                                       >  2:4010409/1‑101 (MQ=255)
                                         cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      <  1:619951/101‑1 (MQ=255)
                                         cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      <  2:3081566/101‑1 (MQ=255)
                                         cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      <  1:4546495/101‑1 (MQ=255)
                                         cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      >  1:3574203/1‑101 (MQ=255)
                                         cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCg                                                      >  1:4047385/1‑101 (MQ=255)
                                          ccGCCCCCTCAGGGAGGGGGGC.GCCCGGGGAAGGCGGGC.AGCCCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                                                     <  1:3246064/101‑1 (MQ=255)
                                           cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                                    <  2:2163813/101‑1 (MQ=255)
                                            ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACg                                                   >  2:12129/1‑101 (MQ=255)
                                                 cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                              <  2:2384346/101‑3 (MQ=255)
                                                 cTCAAGGATGGCGGAGGCCC.GGGAATGAGGG.AAGACCGGGCC.GACCGCAGCCTGTC.TCCGACCTTTTCCCAGCGGAACCCGCGACGGGGGGCGACGCCtgc                                              >  2:1184167/1‑99 (MQ=255)
                                                  tCAGGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                             <  2:874111/101‑4 (MQ=255)
                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcagatc                                             >  1:397666/1‑96 (MQ=255)
                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                             >  1:1938771/1‑98 (MQ=255)
                                                  tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCCtgcg                                             >  2:3037860/1‑98 (MQ=255)
                                                    aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                                          >  2:54916/1‑101 (MQ=255)
                                                     aGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggcg                                         >  1:610679/1‑101 (MQ=255)
                                                      ggATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCC.TGCGgcgc                                        >  1:1673106/1‑101 (MQ=255)
                                                       gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                       <  1:1456730/101‑1 (MQ=255)
                                                       gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                       >  1:4157541/1‑101 (MQ=255)
                                                            cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  2:4295759/101‑1 (MQ=255)
                                                            cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  >  2:1067020/1‑101 (MQ=255)
                                                            cggcgg.cCGGGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  2:3378427/101‑1 (MQ=255)
                                                             ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa                                 >  2:1583132/1‑101 (MQ=255)
                                                               gcggccGGGGAATGCGGGC.AGACGGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa                                <  2:4113781/97‑1 (MQ=255)
                                                                cggccGGGGAATGCGGGCG.GACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaaa                               <  1:1571454/98‑1 (MQ=255)
                                                                 ggccGGGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg                              <  2:4439217/99‑1 (MQ=255)
                                                                 gcCCCGGGAATGCGGG.GAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg                              <  1:3199277/101‑1 (MQ=255)
                                                                  gcccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.g                            <  1:4352167/97‑1 (MQ=255)
                                                                   cccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAa.gg                           >  2:1294960/4‑101 (MQ=255)
                                                                    ccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.gg.g                         >  1:2540442/3‑101 (MQ=255)
                                                                    ccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.gg.g                         <  2:3572766/99‑1 (MQ=255)
                                                                     cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.gg.gg                        <  2:2330531/100‑1 (MQ=255)
                                                                     cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAa.gg.gg                        >  1:72833/2‑101 (MQ=255)
                                                                      gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.gg.ggg                       >  1:1537358/1‑101 (MQ=255)
                                                                      gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg.gg.ggg                       >  1:1667160/1‑101 (MQ=255)
                                                                         aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGGC.TGCGGCGCCGCCCGAAGg.gg.gggtgg                    >  1:2133944/1‑98 (MQ=255)
                                                                         aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTg                    >  1:4641678/1‑101 (MQ=255)
                                                                           tGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTgcg                  <  2:1471892/101‑1 (MQ=255)
                                                                              cgggcagaCCGGGCC.GACCGCCGCCTGTC.TCAGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCCgcgcc                >  1:4274374/6‑100 (MQ=255)
                                                                               cgggcagccGGGGCCGACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCAGCCCGAAAG.GG.GGGCTgcgcg                <  1:394890/94‑1 (MQ=255)
                                                                                 gcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGccc             <  2:629812/99‑1 (MQ=255)
                                                                                   agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGccccc           >  2:3539185/1‑101 (MQ=255)
                                                                                   agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGccccc           <  1:2705122/101‑1 (MQ=255)
                                                                                   agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGccccc           >  1:3364969/1‑101 (MQ=255)
                                                                                    gaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGgccccc          >  2:3217434/1‑101 (MQ=255)
                                                                                     aCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCct         <  1:4687956/101‑1 (MQ=255)
                                                                                      ccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCctc        <  2:2446835/101‑1 (MQ=255)
                                                                                       cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCctct       >  1:1447772/1‑101 (MQ=255)
                                                                                          ggccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGGGGGGCGccccccccctg     >  1:3970625/5‑96 (MQ=255)
                                                                                            ccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCCTCTTggt   <  2:3281279/99‑2 (MQ=38)
                                                                                             cgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCCTCTTggtc  >  2:1579210/2‑99 (MQ=38)
                                                                                             cgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCCTCTTggtc  <  1:182820/100‑3 (MQ=38)
                                                                                              gACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGCGGCGGGCGGCGCGcccccccccccgc   >  1:883535/1‑94 (MQ=255)
                                                                                                   |                                                                                                  
GCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG.GG.GGGCTGCGCGCCCCCCTCTTGGGC  >  NC_014034/1607834‑1608029

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG