Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,644,761 C→T 100% S78F (TCC→TTC)  nuoA → NADH‑quinone oxidoreductase subunit A

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,644,7610C→T100.0% 988.9 253S78F (TCC→TTC) nuoANADH‑quinone oxidoreductase subunit A
Reads supporting (aligned to +/- strand):  new base (143/110):  ref base (0/0):  total (143/110)

Only 100 of 264 total aligned reads displayed.

AAAGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGAATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTCCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTG  >  NC_014034/1644663‑1644858
                                                                                                  |                                                                                                 
 aaGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGa                                                                                                >  1:576022/1‑101 (MQ=255)
 aaGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGa                                                                                                <  1:3994004/101‑1 (MQ=255)
   ggTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATc                                                                                              <  2:4117151/101‑1 (MQ=255)
    gtgtCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCt                                                                                             >  2:1083319/1‑101 (MQ=255)
    gtgtCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCt                                                                                             >  1:2944850/1‑101 (MQ=255)
     tgtCCGCCTATGAATGCGGCTTCAACGCCTTCGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTg                                                                                            <  2:3465456/101‑1 (MQ=255)
      gtCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTgg                                                                                           <  1:2928032/101‑1 (MQ=255)
      gtCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTgg                                                                                           >  1:3627576/1‑101 (MQ=255)
       tCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGa                                                                                          <  1:2737306/101‑1 (MQ=255)
            cTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTgg                                                                                     >  2:3011591/1‑101 (MQ=255)
             tATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGc                                                                                    >  1:440883/1‑101 (MQ=255)
              aTGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCg                                                                                   >  2:182708/1‑101 (MQ=255)
               tGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGt                                                                                  >  2:1658483/1‑101 (MQ=255)
               tGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGt                                                                                  >  2:2562792/1‑101 (MQ=255)
               tGAATGCGGCTTCAACGCC.TTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGtt                                                                                 <  1:1937329/101‑1 (MQ=255)
                gAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGtt                                                                                 >  2:2033048/1‑101 (MQ=255)
                 aaTGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTc                                                                                >  2:4303560/1‑101 (MQ=255)
                   tGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCt                                                                              <  2:3719263/101‑1 (MQ=255)
                    gCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTg                                                                             >  1:911237/1‑101 (MQ=255)
                    gCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTg                                                                             <  2:2957157/101‑1 (MQ=255)
                       gCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTc                                                                          <  1:2404322/101‑1 (MQ=255)
                       gCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTc                                                                          >  1:866839/1‑101 (MQ=255)
                         ttCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTccc                                                                        >  2:3767244/1‑101 (MQ=255)
                          tCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTcccc                                                                       <  2:933657/101‑1 (MQ=255)
                           gagcGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      <  1:4708347/98‑1 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      <  2:223897/101‑1 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      >  1:1218574/1‑101 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      >  2:3370180/1‑101 (MQ=255)
                            aaCGCCTTTGGCGACGCCCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTg                                                                     <  2:4221531/101‑1 (MQ=255)
                            aaCGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTg                                                                     >  2:4421480/1‑101 (MQ=255)
                              cGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTggg                                                                   >  1:4383394/1‑101 (MQ=255)
                                ccTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCg                                                                 >  1:1158705/1‑101 (MQ=255)
                                ccTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCg                                                                 <  1:2537211/101‑1 (MQ=255)
                                 cTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCgg                                                                >  1:2994280/1‑101 (MQ=255)
                                  tttGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGt                                                               >  2:4624063/1‑101 (MQ=255)
                                   ttGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTg                                                              <  2:1972806/101‑1 (MQ=255)
                                    tGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTgg                                                             >  2:76013/1‑101 (MQ=255)
                                     gacgacGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGc                                                            <  2:2437870/101‑1 (MQ=255)
                                      acgacgCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGcc                                                           >  1:2057080/1‑101 (MQ=255)
                                         gcgcgcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCttt                                                        <  2:2903687/100‑1 (MQ=255)
                                         acgCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCttt                                                        <  2:456644/101‑1 (MQ=255)
                                          cgcgcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTg                                                       >  2:3973199/1‑101 (MQ=255)
                                           gcgcgGATGGAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTgg                                                      <  2:650301/101‑1 (MQ=255)
                                            cgcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGc                                                     <  1:4685071/101‑1 (MQ=255)
                                             gcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCg                                                    >  1:373111/1‑101 (MQ=255)
                                              cggaTGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGa                                                   <  1:4750785/98‑1 (MQ=255)
                                               ggaTGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGAc                                                  >  1:2212828/3‑101 (MQ=255)
                                                 aTGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACAt                                                >  1:1492812/1‑101 (MQ=255)
                                                 aTGAAGTTCGACGGGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACAt                                                <  2:4474587/101‑1 (MQ=255)
                                                  tGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATg                                               >  2:3271573/1‑101 (MQ=255)
                                                  ctaaGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATg                                               <  1:678897/99‑1 (MQ=255)
                                                        tCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATg                                         >  1:1662569/1‑101 (MQ=255)
                                                        tCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATg                                         <  2:687099/101‑1 (MQ=255)
                                                         cGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGa                                        >  1:446045/1‑101 (MQ=255)
                                                         cGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGa                                        <  1:4303807/101‑1 (MQ=255)
                                                           aCGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGAcc                                      >  1:646890/1‑101 (MQ=255)
                                                           aCGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGAcc                                      >  2:4313832/1‑101 (MQ=255)
                                                            cGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCg                                     >  2:1774390/1‑101 (MQ=255)
                                                            cGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCg                                     <  2:1251736/101‑1 (MQ=255)
                                                             gTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGc                                    >  1:1080346/1‑101 (MQ=255)
                                                             gTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGc                                    >  1:1700575/1‑101 (MQ=255)
                                                                cgcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCtt                                 <  1:2033854/101‑1 (MQ=255)
                                                                cgcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCtt                                 <  1:416505/101‑1 (MQ=255)
                                                                cgcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTAGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCtt                                 <  2:4714036/101‑1 (MQ=255)
                                                                 gcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACNTGTCGAtnnnnnnnnnn                                >  2:1488198/1‑91 (MQ=255)
                                                                  cTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCt                               <  1:3478051/101‑1 (MQ=255)
                                                                  cTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCt                               <  2:3511066/101‑1 (MQ=255)
                                                                    tttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTgg                             >  1:1991011/1‑101 (MQ=255)
                                                                    tttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTgg                             <  2:4383147/101‑1 (MQ=255)
                                                                    tttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTgg                             <  1:4567579/101‑1 (MQ=255)
                                                                     ttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGt                            <  2:3629475/101‑1 (MQ=255)
                                                                      tATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTc                           >  2:3177881/1‑101 (MQ=255)
                                                                      tATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTc                           <  1:3370386/101‑1 (MQ=255)
                                                                      atcttGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTc                           <  2:2088713/97‑1 (MQ=255)
                                                                       aTCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCg                          <  2:3402564/101‑1 (MQ=255)
                                                                        tCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCga                         >  1:1301416/1‑101 (MQ=255)
                                                                         cTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgat                        >  1:518128/1‑101 (MQ=255)
                                                                         cTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgat                        >  2:2689239/1‑101 (MQ=255)
                                                                          tGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatg                       <  1:754946/101‑1 (MQ=255)
                                                                          tGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatg                       <  1:1889040/101‑1 (MQ=255)
                                                                          cgatctCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatg                       <  1:276396/96‑1 (MQ=255)
                                                                           ggTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatga                      >  2:192654/1‑101 (MQ=255)
                                                                           ggTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatga                      >  1:1638023/1‑101 (MQ=255)
                                                                           ggTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatga                      >  1:3163974/1‑101 (MQ=255)
                                                                               tCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgt                  >  2:2132133/1‑101 (MQ=255)
                                                                               tCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgt                  >  2:3244701/1‑101 (MQ=255)
                                                                                cGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatgagatcg                 >  2:276362/1‑96 (MQ=255)
                                                                                 gATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtgt                <  2:512377/101‑1 (MQ=255)
                                                                                 gATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtgt                <  2:3061824/101‑1 (MQ=255)
                                                                                 gATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtgt                <  2:349321/101‑1 (MQ=255)
                                                                                    ccTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTTTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTcc             <  1:902736/101‑1 (MQ=255)
                                                                                     cTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCct            <  1:3331576/101‑1 (MQ=255)
                                                                                       gTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGTTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCctct          >  2:4102100/1‑101 (MQ=255)
                                                                                       gTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCctct          >  1:2281131/1‑101 (MQ=255)
                                                                                        ttCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCtctc         >  2:623200/1‑101 (MQ=255)
                                                                                         tcatcaTCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCg        >  2:1026919/1‑101 (MQ=255)
                                                                                          catcatCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCgg       >  1:3786902/1‑101 (MQ=255)
                                                                                          catcatCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCgg       >  2:3575880/1‑101 (MQ=255)
                                                                                           atcatcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGt      >  2:3845263/1‑101 (MQ=255)
                                                                                           atcatcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGt      <  2:1218484/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AAAGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGAATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTCCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTG  >  NC_014034/1644663‑1644858

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG