Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,733 C→T 100% S477N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,7330C→T100.0% 964.7 246S477N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (124/122):  ref base (0/0):  total (124/122)

Only 100 of 256 total aligned reads displayed.

CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830
                                                                                                  |                                                                                                 
ccATTTTCACGAAATCGGGCAGGCTTTCCGCCCAGCTTTCCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTg                                                                                                 <  1:4385267/101‑1 (MQ=255)
  aGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGtt                                                                                               <  1:3826046/101‑1 (MQ=255)
  aGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTGGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGtt                                                                                               <  1:3249970/101‑1 (MQ=255)
  aGTTTCACGAAAGCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGtt                                                                                               <  2:3258526/101‑1 (MQ=255)
     ttCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGACGCACCATGCCCAGCCGCTCGCTGTTCAg                                                                                            >  2:1585174/1‑101 (MQ=255)
      tCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAgg                                                                                           >  2:117309/1‑101 (MQ=255)
       cACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGa                                                                                          >  1:4656712/1‑101 (MQ=255)
          gAAAGCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGa                                                                                       <  2:828420/101‑1 (MQ=255)
           aaacggggCGGGCTTCCCGACCAGCTTTGCAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      <  2:1956242/96‑1 (MQ=255)
             aTCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACt                                                                                    >  2:1549212/1‑101 (MQ=255)
             aTCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACt                                                                                    >  1:4022680/1‑101 (MQ=255)
             aTCGGGCAGGCTTTCCGACCAGCTTGGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACt                                                                                    <  1:3584764/101‑1 (MQ=255)
              tCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTg                                                                                   <  1:4426572/101‑1 (MQ=255)
              tCGGGCAGGCTTCCCGCCCAGCTTGGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTg                                                                                   <  2:4295260/101‑1 (MQ=255)
              gcGGGCAGGCTTCCCGCCCAGTTTTGCAAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTg                                                                                   <  2:2474637/100‑1 (MQ=255)
               cGGGCAGGCTTTCCGACAAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGc                                                                                  >  2:2640105/1‑101 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 >  1:1518620/1‑101 (MQ=255)
                  gttgccTTCCCGCCCTGCTTTTGCAAAAACGCCCGCCATGCAGCGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTg                                                                               <  1:2309253/96‑1 (MQ=255)
                  gCAGGCTTTCCGACCAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTg                                                                               <  1:1229684/101‑1 (MQ=255)
                    aGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAc                                                                             >  1:2395564/1‑101 (MQ=255)
                    aGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAc                                                                             >  1:4438001/1‑101 (MQ=255)
                     ggCTTTCCGCCCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAcc                                                                            <  2:3822830/101‑1 (MQ=255)
                     ggCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAcc                                                                            <  1:4361779/101‑1 (MQ=255)
                      gCTTTCCGACCAGCTTTGCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCg                                                                           <  2:351574/101‑1 (MQ=255)
                         ttCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgggg                                                                        >  2:1316457/1‑101 (MQ=255)
                          tCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGt                                                                       >  1:1137979/1‑101 (MQ=255)
                           ccGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      >  1:2488308/1‑101 (MQ=255)
                           ccGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      >  2:2573209/1‑101 (MQ=255)
                           ccGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      >  2:178531/1‑101 (MQ=255)
                            cGACCAGCTTTGCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGc                                                                     <  1:1948354/101‑1 (MQ=255)
                            cGACCAGCTTTGCAAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGc                                                                     <  2:3233823/101‑1 (MQ=255)
                             gACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCg                                                                    >  2:4240077/1‑101 (MQ=255)
                               ccAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGa                                                                  >  1:563457/1‑101 (MQ=255)
                               ccAGCTTTGCGAAAACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGa                                                                  <  1:1487267/101‑1 (MQ=255)
                                cAGTTTTGCAAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 <  2:3451157/101‑1 (MQ=255)
                                cAGCTTTGCGAATACCGCCGCCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 <  2:604504/101‑1 (MQ=255)
                                 aGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAc                                                                >  1:2258842/1‑101 (MQ=255)
                                   cTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTg                                                              >  1:3704935/1‑101 (MQ=255)
                                   cTTTGCAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTg                                                              <  2:3118054/101‑1 (MQ=255)
                                    tttGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             >  2:2364767/1‑101 (MQ=255)
                                    tttGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             >  2:663171/1‑101 (MQ=255)
                                    tttGCCAATACGGCCCGCCATGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             <  1:1585273/101‑1 (MQ=255)
                                     ttGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCg                                                            >  2:3037467/1‑101 (MQ=255)
                                      ggcaaaTACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGt                                                           <  1:3327523/97‑1 (MQ=255)
                                        ggAATACCGCCCGCCGTGCAGCAGGTCCTCCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCg                                                         <  1:210577/100‑1 (MQ=255)
                                         gaaaaccccccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGc                                                        <  2:4494866/93‑1 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  2:4123614/1‑101 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  2:1815248/1‑101 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  1:1087494/1‑101 (MQ=255)
                                           aTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCg                                                      >  1:3918239/1‑101 (MQ=255)
                                              aCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGc                                                   >  2:2177252/1‑101 (MQ=255)
                                                ccccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  1:3739390/100‑1 (MQ=255)
                                                 cccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCa                                                >  1:959017/1‑101 (MQ=255)
                                                 cccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGAGCGGAAATGCGTCGCAGTGccaa                                                >  2:3655173/1‑99 (MQ=255)
                                                  ccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               >  2:1737660/1‑101 (MQ=255)
                                                  ccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               <  2:1209589/101‑1 (MQ=255)
                                                    ccctggcggcagGTCCTGCCCCTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCGGTGCCCAttt                                             <  2:1010606/93‑1 (MQ=255)
                                                    cccATGCGGAAGGTCCGGCCCCTGCCGCACCATGCCCAGCCGCTCGTTGTTCTGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             <  1:1700052/100‑1 (MQ=255)
                                                      cATGCAGGAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           <  1:2655864/101‑1 (MQ=255)
                                                      cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  1:2887454/1‑101 (MQ=255)
                                                      cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  1:3537120/1‑101 (MQ=255)
                                                         gcagcaGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        >  2:101735/1‑101 (MQ=255)
                                                              gggTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGtt                                   <  1:98831/100‑1 (MQ=255)
                                                              agGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGtt                                   >  2:402473/1‑101 (MQ=255)
                                                               ggTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtc                                  >  1:295308/1‑101 (MQ=255)
                                                                 cccTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcat                                <  1:4698968/100‑1 (MQ=255)
                                                                   cTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              <  2:4309710/101‑1 (MQ=255)
                                                                    tcccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  2:3704705/99‑1 (MQ=255)
                                                                    tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             >  1:138077/1‑101 (MQ=255)
                                                                    tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  1:3436064/101‑1 (MQ=255)
                                                                    tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             >  1:4234555/1‑101 (MQ=255)
                                                                        ccTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAg                         <  2:4196627/100‑1 (MQ=255)
                                                                        aCTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAg                         >  2:4089950/1‑101 (MQ=255)
                                                                         cTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        <  1:3863864/101‑1 (MQ=255)
                                                                         cTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        <  2:4437751/101‑1 (MQ=255)
                                                                          tGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGa                       <  2:3377403/101‑1 (MQ=255)
                                                                           gCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGAc                      >  2:3944834/1‑101 (MQ=255)
                                                                           gCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGAc                      <  1:663235/101‑1 (MQ=255)
                                                                            ccGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACg                     <  1:4157004/101‑1 (MQ=255)
                                                                            ccGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACg                     >  2:1473450/1‑101 (MQ=255)
                                                                               cACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCt                  >  1:2846284/1‑101 (MQ=255)
                                                                                cccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTc                 <  2:4668670/100‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  1:1000113/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGGGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                >  1:1429594/1‑101 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              <  2:1679857/101‑1 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              >  2:2874888/1‑101 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              <  2:3315956/101‑1 (MQ=255)
                                                                                     gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  2:2757709/1‑101 (MQ=255)
                                                                                     gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  2:2085353/1‑101 (MQ=255)
                                                                                     gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  2:4552743/1‑101 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  1:3037655/101‑1 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  1:1774978/101‑1 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCAGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  1:4123856/101‑1 (MQ=255)
                                                                                          gCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATg       <  2:958940/101‑1 (MQ=255)
                                                                                           ccGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGt      >  2:2720437/1‑101 (MQ=255)
                                                                                            cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  2:2000714/101‑1 (MQ=255)
                                                                                            cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  1:847192/101‑1 (MQ=255)
                                                                                             gctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTg    <  2:2106368/101‑1 (MQ=255)
                                                                                               tcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAt  >  2:2804531/1‑101 (MQ=255)
                                                                                               tcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAt  >  2:3164417/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG