Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,994 C→T 100% S390N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,9940C→T100.0% 811.4 208S390N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (109/98):  ref base (1/0):  total (110/98)

Only 100 of 238 total aligned reads displayed.

CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCC  >  NC_014034/1668896‑1669090
                                                                                                  |                                                                                                
ccAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTg                                                                                                >  2:2055102/1‑101 (MQ=255)
 cAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgc                                                                                               >  1:1769990/1‑101 (MQ=255)
       tCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGCTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCt                                                                                         >  1:3547415/1‑101 (MQ=255)
       tCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCt                                                                                         >  2:1763078/1‑101 (MQ=255)
        ccGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtt                                                                                        <  2:4620348/101‑1 (MQ=255)
        ccGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtt                                                                                        <  1:4373057/101‑1 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                       <  1:2100570/101‑1 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                       >  2:1212388/1‑101 (MQ=255)
          gCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTgg                                                                                      <  2:1490860/101‑1 (MQ=255)
          gCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTgg                                                                                      >  1:220657/1‑101 (MQ=255)
           cTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGt                                                                                     >  2:3323512/1‑101 (MQ=255)
           cTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGt                                                                                     <  2:4510575/101‑1 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTc                                                                                    >  2:3672016/1‑101 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTc                                                                                    <  1:2460885/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCa                                                                                   >  2:1963904/1‑101 (MQ=255)
               tCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAgg                                                                                 >  1:4733322/1‑101 (MQ=255)
               tCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAgg                                                                                 <  2:3545933/101‑1 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                               <  1:4090192/101‑1 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                               >  1:395683/1‑101 (MQ=255)
                  tCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGcc                                                                              <  1:2082443/101‑1 (MQ=255)
                   ccAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCt                                                                             >  1:229573/1‑101 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                           <  1:2875064/101‑3 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                           <  1:139851/101‑3 (MQ=255)
                     aGCGGTTCGGCATCTCGAATCCCAGATATTGCGCCGCCCACAACTGTTTCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCTCCCCTTGGTCAGGGCCtcc                                                                           <  2:1091123/101‑3 (MQ=255)
                      gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                          <  1:2055223/101‑1 (MQ=255)
                      gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                          <  2:3574251/101‑1 (MQ=255)
                       cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                         <  1:833030/101‑1 (MQ=255)
                        ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                        <  1:2384053/101‑1 (MQ=255)
                        ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGAGATGTAGCGGTTGCCCCCCTTGGTCAGGGCCTCCAGc                                                                        >  1:4481713/1‑101 (MQ=255)
                         gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                       <  2:4378228/101‑1 (MQ=255)
                          ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                      <  2:3994219/101‑1 (MQ=255)
                          ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                      >  1:106576/1‑101 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGGGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                    >  1:3287695/1‑101 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                    >  1:2952318/1‑101 (MQ=255)
                             ggCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                   >  2:2520101/1‑101 (MQ=255)
                              gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                  <  2:1822102/101‑1 (MQ=255)
                              gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                  <  1:413593/101‑1 (MQ=255)
                               cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                 <  2:4226338/101‑1 (MQ=255)
                               cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                 <  1:665737/101‑1 (MQ=255)
                                gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                >  2:582927/1‑101 (MQ=255)
                                gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                >  2:1925119/1‑101 (MQ=255)
                                gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                <  2:263605/101‑1 (MQ=255)
                                gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                <  1:534105/101‑1 (MQ=255)
                                    cGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                            >  2:4017637/1‑101 (MQ=255)
                                    cGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                            <  2:3212591/101‑1 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                          <  1:3098786/101‑1 (MQ=255)
                                       aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                         >  2:2264446/1‑101 (MQ=255)
                                         cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                       <  1:2777685/101‑1 (MQ=255)
                                            aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                    >  2:3409882/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                  >  2:1334922/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGCGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTTCGGtt                                                  >  2:553064/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGAGGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                  <  2:939260/101‑1 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                 <  2:2105998/101‑1 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                 <  1:1887422/101‑1 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                 <  1:3786665/101‑1 (MQ=255)
                                                atTGCGCCGCCCTCACCTGATGCGGGCCGACTTCGGTGGTGAGGTAGCGGTTGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                <  2:4682676/101‑1 (MQ=255)
                                                 ttGCCCCGCCCACATCTGATGCGGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                               <  2:4753802/101‑2 (MQ=255)
                                                     gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                           <  2:3010998/101‑1 (MQ=255)
                                                     gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                           <  1:3808430/101‑1 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                        >  2:1904097/1‑101 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                        <  1:4108821/101‑1 (MQ=255)
                                                            acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                    >  2:2473014/1‑101 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                   <  2:851694/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                   >  1:125610/1‑101 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                   <  1:1431861/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                   <  1:74608/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                   >  1:1682416/1‑101 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                 <  1:3717162/101‑1 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                 >  1:4228766/1‑101 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                 <  1:4520684/101‑1 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                 <  1:1964021/101‑1 (MQ=255)
                                                                 tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                               >  1:3148969/1‑101 (MQ=255)
                                                                  gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                              >  2:1136524/1‑101 (MQ=255)
                                                                  gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                              >  1:3404272/1‑101 (MQ=255)
                                                                      cTGGCGGACTTCGGTGGTGATTTGGCGGTTGCCCCCCTGGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                          <  1:848814/101‑1 (MQ=255)
                                                                       tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                         >  1:2429530/1‑101 (MQ=255)
                                                                        ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                        >  2:3438196/1‑101 (MQ=255)
                                                                         gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                       <  2:2956183/101‑1 (MQ=255)
                                                                         gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                       >  1:4671931/1‑101 (MQ=255)
                                                                          ccGACTTCGGTGGTGATGTAGGGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                      <  2:2452399/101‑1 (MQ=255)
                                                                          ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                      <  2:2777055/101‑1 (MQ=255)
                                                                           cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                     >  1:2728428/1‑101 (MQ=255)
                                                                               ttgggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                 <  1:632377/98‑1 (MQ=255)
                                                                                tCGGTGGTTATGTAGGGGTTGCCCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                <  1:4412303/101‑1 (MQ=255)
                                                                                tCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                >  2:1984278/1‑101 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               >  2:1179078/1‑101 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               >  1:199826/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg            >  2:1385488/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg            >  2:1633935/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg            >  1:545471/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg            >  2:4093323/1‑101 (MQ=255)
                                                                                     ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa           >  1:2523405/1‑101 (MQ=255)
                                                                                       tgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc         >  2:4248326/1‑101 (MQ=255)
                                                                                        gATGTGGCGGTTGCGCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct        <  2:650806/101‑1 (MQ=255)
                                                                                         aTGTAGCGGTTGCGCCCTTGGGCCAGGCCCGCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt       <  2:3908964/101‑1 (MQ=255)
                                                                                         aTGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt       >  2:726902/1‑101 (MQ=255)
                                                                                          tGTAGCGTTTGCGCCCTTGGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc      <  2:3732482/101‑1 (MQ=255)
                                                                                          tGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc      >  2:3151723/1‑101 (MQ=255)
                                                                                           gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct     >  1:1323962/1‑101 (MQ=255)
                                                                                            tAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt    >  1:1837258/1‑101 (MQ=255)
                                                                                                  |                                                                                                
CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCC  >  NC_014034/1668896‑1669090

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG