Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 794.9 205K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (108/97):  ref base (0/0):  total (108/97)

Only 100 of 231 total aligned reads displayed.

GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCAC  >  NC_014034/1668918‑1669110
                                                                                                  |                                                                                              
gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                              <  2:3574251/101‑1 (MQ=255)
 cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                                             >  2:1201121/1‑101 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                            <  2:845582/101‑1 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                           <  2:4378228/101‑1 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                           <  1:2047770/101‑1 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                          >  1:106576/1‑101 (MQ=255)
      cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGGGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                        >  1:3287695/1‑101 (MQ=255)
      cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                        >  1:2952318/1‑101 (MQ=255)
        gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                      >  2:2478309/1‑101 (MQ=255)
        gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                      <  1:413593/101‑1 (MQ=255)
         cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                                     <  2:4226338/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                    >  2:1925119/1‑101 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                    >  2:582927/1‑101 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                    <  2:263605/101‑1 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                   >  2:1574411/1‑101 (MQ=255)
            cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                                                  <  2:3075426/101‑1 (MQ=255)
              cGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                                                >  2:4017637/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                               >  1:1977785/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                              >  2:4749098/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                              >  1:150635/1‑101 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                             <  1:2364900/101‑1 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                             <  1:2120717/101‑1 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                             <  2:3435262/101‑1 (MQ=255)
                  gCCCAGATATGGCGCCCCCCACATCTGATGCTGGCCGACTTCGGTGGTAATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                                            <  2:3547196/101‑1 (MQ=255)
                   cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                           <  1:4305747/101‑1 (MQ=255)
                   cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                           <  1:1411533/101‑1 (MQ=255)
                      aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                                        >  1:1280065/1‑101 (MQ=255)
                      aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                                        >  1:2070098/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                     <  1:3786665/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                     >  2:3381082/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                     <  1:1887422/101‑1 (MQ=255)
                          atTGCGCCGCCCTCACCTGATGCGGGCCGACTTCGGTGGTGAGGTAGCGGTTGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                    <  2:4682676/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                    <  2:2182621/101‑1 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                  >  2:2115348/1‑99 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                  >  2:1920029/1‑99 (MQ=255)
                             gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                 <  1:2686157/101‑1 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                               <  1:3808430/101‑1 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                               <  2:3010998/101‑1 (MQ=255)
                                   cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                                           <  1:3672245/101‑1 (MQ=255)
                                    ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                                          <  1:2783260/101‑1 (MQ=255)
                                       caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                       <  1:4453127/101‑1 (MQ=255)
                                       caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                       <  2:1975706/101‑1 (MQ=255)
                                         tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                                     >  1:4228766/1‑101 (MQ=255)
                                          cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                                    >  2:3995652/1‑101 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                   >  1:3148969/1‑101 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                  >  1:3404272/1‑101 (MQ=255)
                                             aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                                                 >  1:1707347/1‑101 (MQ=255)
                                              tgcggcccGCCTTCGTTGGTGTTGTAGCGGTGGCGCCCCTTGATCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                                                <  2:1875156/95‑1 (MQ=255)
                                              tGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGAGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                                                >  2:787874/1‑101 (MQ=255)
                                               gCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtg                                               <  1:3049813/101‑1 (MQ=255)
                                                cTGGCGGACTTCGGTGGTGATTTGGCGGTTGCCCCCCTGGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                                              <  1:848814/101‑1 (MQ=255)
                                                 tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                             >  2:1103385/1‑101 (MQ=255)
                                                 tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                             >  1:2429530/1‑101 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTGGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                            <  2:113937/101‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                            <  2:3196824/101‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                            <  1:448743/101‑1 (MQ=255)
                                                   gCCGCCTTCGGTGGTGAGGTAGCGGTTGCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                                           <  1:1341950/101‑1 (MQ=255)
                                                   gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                                           >  2:1341858/1‑101 (MQ=255)
                                                   gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                                           >  1:4671931/1‑101 (MQ=255)
                                                    ccGATTTGGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                                          <  2:2457832/101‑1 (MQ=255)
                                                    ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                                          <  2:2777055/101‑1 (MQ=255)
                                                    ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                                          >  1:3554340/1‑101 (MQ=255)
                                                    ccGACTCGGGTGTTGTGGTAGCGGTTGCGCCCCTTGTTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                                          <  2:881109/101‑1 (MQ=255)
                                                        cTTCGGTGGTGATGTAGCGGTTGCGCCCTTTGTTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                                      <  1:57221/101‑1 (MQ=255)
                                                         ttgggtgttGATGTGGGGTTTGCGCCCCTTGGTGGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                     <  2:2930201/98‑1 (MQ=255)
                                                         ttgggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                     <  1:632377/98‑1 (MQ=255)
                                                         ttggggggtgATGTAGCGGTGGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGaaat                                     <  2:1707242/95‑4 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                  >  1:3732712/1‑101 (MQ=255)
                                                              tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                >  2:4093323/1‑101 (MQ=255)
                                                              tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                >  1:545471/1‑101 (MQ=255)
                                                              tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                >  1:815907/1‑101 (MQ=255)
                                                                gtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                              >  2:208173/1‑101 (MQ=255)
                                                                gtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCAGAt                              >  1:527702/1‑101 (MQ=255)
                                                                  gATGTGGCGGTTGCGCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                            <  2:650806/101‑1 (MQ=255)
                                                                    tGTAGGGGTTGCCCCCCTTGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                          <  1:3988759/101‑1 (MQ=255)
                                                                    tGTAGCGTTTGCGCCCTTGGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                          <  2:3732482/101‑1 (MQ=255)
                                                                     gtgggggttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                         <  1:805494/93‑1 (MQ=255)
                                                                      tAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt                        >  1:1837258/1‑101 (MQ=255)
                                                                      tAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGGTTGATGATGCTGTCCGAGCCGGTGCAGGCGAGGCAGCGGATcttctt                        >  2:4528761/1‑101 (MQ=255)
                                                                       aggggttGCCCCCCTTGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                       <  1:3932297/95‑1 (MQ=255)
                                                                       aGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                       >  1:741500/1‑101 (MQ=255)
                                                                        ggggtcgcgcCCCTTGGTCAGGGCCTCCAGCCGCTCAAGCGCATTTTGCGGTTTGAAGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTcc                      <  2:565656/95‑1 (MQ=255)
                                                                          ggttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAc                    >  2:2003517/4‑101 (MQ=255)
                                                                          ggttGCCCCCTTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAc                    <  2:2303555/98‑1 (MQ=255)
                                                                            ttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                  >  2:2492462/2‑101 (MQ=255)
                                                                            ttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGAGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                  >  2:3828115/2‑101 (MQ=255)
                                                                            ttGCCCCCCTTGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                  <  2:1323870/100‑1 (MQ=255)
                                                                             tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                 >  2:2294393/1‑101 (MQ=255)
                                                                              gcgcCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcct                >  2:3673125/1‑101 (MQ=255)
                                                                               ccccctTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc               <  1:2408483/99‑1 (MQ=255)
                                                                                gcCCCTGGGTGCGGACCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg              <  1:4749354/101‑1 (MQ=255)
                                                                                  cccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt            >  1:4582657/1‑101 (MQ=255)
                                                                                  cccTGGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt            <  1:1212478/101‑1 (MQ=255)
                                                                                    cTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt          <  2:3314498/101‑1 (MQ=255)
                                                                                     tgggccggggCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt         <  2:395639/94‑1 (MQ=255)
                                                                                      tGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg        >  2:522668/1‑101 (MQ=255)
                                                                                      tGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg        <  2:4304813/101‑1 (MQ=255)
                                                                                        gTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcc      >  2:3064007/1‑101 (MQ=255)
                                                                                        gTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcc      >  2:1504828/1‑101 (MQ=255)
                                                                                            ggcccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccac  <  1:690308/98‑1 (MQ=255)
                                                                                                  |                                                                                              
GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCAC  >  NC_014034/1668918‑1669110

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG