Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,181 T→A 3.8% intergenic (‑109/+116) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1810T→A 3.8% 14.8 ‑2.5 ‑0.0235intergenic (‑109/+116)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (7/2):  ref base (101/125):  total (108/127)
Fisher's exact test strand distribution p-value = 8.42e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

Only 100 of 242 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.C.AATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283
                                                                                                     |                                                                                                      
 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CTAATTca                                                                                                     >  2:3324462/1‑100 (MQ=255)
   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCAcc                                                                                                  >  1:1730017/1‑101 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCAccc                                                                                                 <  1:2971853/101‑1 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCAcccc                                                                                                <  1:1126888/101‑2 (MQ=37)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCAcccc                                                                                                >  2:2406485/1‑100 (MQ=255)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCAcccccg                                                                                              >  2:1341969/1‑98 (MQ=37)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCTCGCCTTTCGGCC.CATTCACCCCGGGa                                                                                             >  1:186739/1‑101 (MQ=25)
          tGAATTTAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGat                                                                                            <  2:1152016/101‑2 (MQ=255)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGat                                                                                            <  2:651641/101‑2 (MQ=255)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGat                                                                                            >  1:2765204/1‑100 (MQ=37)
           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGatg                                                                                           <  1:4090014/101‑3 (MQ=25)
            aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGatgt                                                                                          <  1:3610571/101‑4 (MQ=255)
            aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.C.CAATTCACCCCGGGAGGt                                                                                         >  1:1269887/1‑101 (MQ=255)
            aaTTGAACGTCTGTTTAATTTAGAATCCTTGAACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGatgt                                                                                          >  1:2699879/1‑98 (MQ=255)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGatgtt                                                                                         <  1:2224912/101‑5 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTg                                                                                       <  1:710718/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTgg                                                                                      <  2:3179951/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTgg                                                                                      <  2:4478891/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTgg                                                                                      >  1:4372930/1‑101 (MQ=25)
                 aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTggg                                                                                     >  1:3949002/1‑101 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGcc                                                                                   <  1:1725647/101‑1 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGcc                                                                                   >  2:3068815/1‑101 (MQ=25)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCa                                                                                  <  2:3264884/101‑1 (MQ=25)
                     tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaa                                                                                 >  2:2887537/1‑101 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCCCCCCCGGTTGTTTGGGCCatg                                                                                >  2:4744232/1‑99 (MQ=19)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGAGC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCAaag                                                                                >  2:4590206/1‑100 (MQ=255)
                       tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaagg                                                                               <  1:3908399/101‑3 (MQ=19)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggt                                                                              <  1:928700/101‑4 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             <  1:4719475/101‑5 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            <  1:571282/101‑6 (MQ=16)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            >  1:4652297/1‑96 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            <  1:2432864/101‑6 (MQ=16)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  1:3531895/101‑7 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  1:2939372/101‑7 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  2:1171172/101‑8 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  2:672659/101‑8 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                       >  2:1888661/1‑101 (MQ=255)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTTGGTCAA..GGTGAAacacct                                                                   >  2:3486337/1‑97 (MQ=255)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  <  1:782048/101‑6 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 >  2:4522496/1‑95 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 <  2:468101/101‑7 (MQ=12)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  2:2765040/101‑10 (MQ=12)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              >  1:2591201/1‑92 (MQ=255)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  1:4234604/101‑10 (MQ=9)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              >  2:3613016/1‑92 (MQ=255)
                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                        <  2:2563312/101‑1 (MQ=255)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                      <  1:1057439/101‑1 (MQ=9)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     >  2:3849871/1‑101 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  2:4117171/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:1365413/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:3842979/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:653614/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGCGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  2:4266530/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                    <  2:509463/101‑1 (MQ=255)
                                                aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                  <  2:1926297/101‑1 (MQ=255)
                                                 ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                 >  2:2030324/1‑101 (MQ=9)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                >  1:1940386/1‑101 (MQ=255)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                >  1:2371884/1‑101 (MQ=255)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                >  1:3023257/1‑101 (MQ=255)
                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                              >  2:4299910/1‑101 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                             >  1:4199260/1‑101 (MQ=255)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                             <  2:1727680/101‑1 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTG.TTTCA.CCTTGCTTTTTCTTGGCGCAAAt                                             <  2:3028697/101‑1 (MQ=2)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                            <  1:1334362/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc                                           <  1:4300157/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           >  2:4569099/1‑101 (MQ=9)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           >  1:1508644/1‑101 (MQ=9)
                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                          <  1:4175932/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       <  2:1293761/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       <  1:3767219/101‑1 (MQ=9)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                     <  2:4372683/101‑1 (MQ=255)
                                                                   gaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  2:1813504/92‑1 (MQ=9)
                                                                   gaggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  2:835501/92‑1 (MQ=9)
                                                                    aggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  1:1119077/93‑1 (MQ=255)
                                                                    aggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                >  2:179491/9‑101 (MQ=16)
                                                                    aggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  2:2097078/93‑1 (MQ=255)
                                                                     ggggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                               >  2:2633761/8‑101 (MQ=12)
                                                                      gggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                              >  1:1666108/7‑101 (MQ=16)
                                                                       ggcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                             <  1:2806646/96‑1 (MQ=16)
                                                                        gcgctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                            <  1:1582882/97‑1 (MQ=255)
                                                                          gctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                          <  1:3324664/99‑1 (MQ=255)
                                                                           ctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                         <  1:3661463/100‑1 (MQ=255)
                                                                           ctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                         <  2:418517/100‑1 (MQ=255)
                                                                           ctGCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                         <  2:3821435/100‑1 (MQ=255)
                                                                             gCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       <  2:2964375/101‑1 (MQ=255)
                                                                             gCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       <  1:421007/101‑1 (MQ=255)
                                                                             gCCCCTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       >  1:3388995/1‑101 (MQ=19)
                                                                                ccTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                    <  2:368081/101‑1 (MQ=255)
                                                                                 cTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                   <  1:4029361/101‑1 (MQ=19)
                                                                                 cTCCTGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                   <  1:2242794/101‑1 (MQ=255)
                                                                                    ctGGCCTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc                <  2:142872/100‑1 (MQ=19)
                                                                                        ccTTTCGGCC.AATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            <  2:1236462/101‑1 (MQ=19)
                                                                                        ccTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            <  1:1923404/101‑1 (MQ=19)
                                                                                         cTTTCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           >  2:2162204/1‑101 (MQ=255)
                                                                                           ttCGGCC.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc         >  1:265520/1‑101 (MQ=255)
                                                                                            tcggcc.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg        >  2:2583068/6‑101 (MQ=255)
                                                                                            tcggcc.AATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg        >  2:2299640/6‑101 (MQ=255)
                                                                                              ggcc.AATTCACCCCGGGATGTTTGGGCCAA..GGCGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg      >  1:1643640/4‑101 (MQ=19)
                                                                                                   aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc  <  2:737674/101‑1 (MQ=25)
                                                                                                   aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc  >  1:4413879/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.C.AATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG