Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,751,385 C→G 100% A230G (GCG→GGG)  RCAP_rcc01619 → Fis family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,751,3850C→G100.0% 598.1 157A230G (GCG→GGG) RCAP_rcc01619Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (88/69):  ref base (0/0):  total (88/69)

Only 100 of 164 total aligned reads displayed.

CAGGATGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGCGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGCCGG  >  NC_014034/1751288‑1751482
                                                                                                 |                                                                                                 
cAGGATGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGggt                                                                                                <  2:3810899/101‑1 (MQ=255)
 aGGATGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGgggg                                                                                               >  2:3099489/1‑99 (MQ=255)
 aGGATGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGgggg                                                                                               >  1:4742655/1‑99 (MQ=255)
    gtggggCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGGGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGgtggtg                                                                                            <  1:4239169/97‑1 (MQ=255)
    aTGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGgtggtg                                                                                            <  2:2750246/101‑1 (MQ=255)
        ggCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTc                                                                                        <  1:4371289/101‑1 (MQ=255)
         gCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCt                                                                                       >  1:1307701/1‑101 (MQ=255)
         gCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCt                                                                                       <  2:4241472/101‑1 (MQ=255)
          ccAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTg                                                                                      >  1:2744470/1‑101 (MQ=255)
          ccAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGGGGTGCTTCTg                                                                                      >  1:3981452/1‑101 (MQ=255)
             gCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGgcg                                                                                   >  1:532521/1‑101 (MQ=255)
              ccGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGgcgc                                                                                  >  2:4583950/1‑101 (MQ=255)
                ggATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCtt                                                                                >  1:3829659/1‑101 (MQ=255)
                 gATCGAGGCGGATCTTTCCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTg                                                                               <  2:188573/101‑1 (MQ=255)
                 gATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTg                                                                               <  1:4456293/101‑1 (MQ=255)
                  gtCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGa                                                                              <  1:2821195/100‑1 (MQ=255)
                    cGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGAcc                                                                            <  1:880748/101‑1 (MQ=255)
                    cGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGAcc                                                                            <  1:1071721/101‑1 (MQ=255)
                    cGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGAcc                                                                            >  2:2769275/1‑101 (MQ=255)
                     gAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACcg                                                                           >  1:1751206/1‑101 (MQ=255)
                      aGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGGTTCTGGCGCTTGACcgc                                                                          >  2:4269010/1‑101 (MQ=255)
                      aGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACcgc                                                                          >  2:2878966/1‑101 (MQ=255)
                      aGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGGGGTGCTTCTGGCGCTTGACcgc                                                                          >  1:3015705/1‑101 (MQ=255)
                       ggctgttCTGTTCCGCGCCTCCTTCGAGGGGGCGCTGATCCTCTCGACCTGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGTTTCTTGCGCTTGACcgcg                                                                         <  2:4449856/95‑1 (MQ=255)
                         cGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGcgac                                                                       >  1:3075165/1‑101 (MQ=255)
                          ggATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGcgacg                                                                      <  1:2973796/101‑1 (MQ=255)
                          ggATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGcgacg                                                                      >  1:1250079/1‑101 (MQ=255)
                             tCTGTTCCGCGCCTCCTNCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATc                                                                   <  2:575308/101‑1 (MQ=255)
                              cTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCt                                                                  >  2:3620040/1‑101 (MQ=255)
                               tGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTg                                                                 <  2:3135589/101‑1 (MQ=255)
                                 ttCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGt                                                               >  1:1906681/1‑101 (MQ=255)
                                 ttCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGGGGTGCTTCTGGCGCTTGACCGCGACGATCTGGt                                                               >  1:846838/1‑101 (MQ=255)
                                  tCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTg                                                              <  1:2940332/101‑1 (MQ=255)
                                   tcgcgCCTCCTTCGAGGGGGCGCGGAGCCTCTCGACCGGAGCGGGCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGa                                                             <  1:2857557/100‑1 (MQ=255)
                                    cgcgCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGAt                                                            <  2:2046620/101‑1 (MQ=255)
                                    cgcgCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGAt                                                            <  2:2744306/101‑1 (MQ=255)
                                    cgcgCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGAt                                                            <  2:1336189/101‑1 (MQ=255)
                                     gcgcCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATc                                                           <  1:1477305/101‑1 (MQ=255)
                                     gcgcCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATc                                                           >  1:1621958/1‑101 (MQ=255)
                                      cgcCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCg                                                          <  2:2647067/101‑1 (MQ=255)
                                      cgcCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCg                                                          <  2:1084673/101‑1 (MQ=255)
                                         ctcctTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGgcg                                                       <  1:974334/101‑1 (MQ=255)
                                           cctTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGcgcg                                                     <  2:3829424/101‑1 (MQ=255)
                                            ctTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGa                                                    >  1:2309693/1‑101 (MQ=255)
                                             ttCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAc                                                   >  2:81152/1‑101 (MQ=255)
                                             ttCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAc                                                   >  2:2952333/1‑101 (MQ=255)
                                              tCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcg                                                  >  1:3668134/1‑101 (MQ=255)
                                              tCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcg                                                  >  1:2276699/1‑101 (MQ=255)
                                               cGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGGAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcgc                                                 >  2:4103931/1‑101 (MQ=255)
                                               cGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcgc                                                 >  2:154708/1‑101 (MQ=255)
                                                gAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcgcg                                                >  1:3688216/1‑101 (MQ=255)
                                                gAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcgcg                                                >  1:2633909/1‑101 (MQ=255)
                                                gAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGAcgcg                                                >  1:3681508/1‑101 (MQ=255)
                                                  gggggCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACgcggg                                              >  2:3001860/1‑101 (MQ=255)
                                                   ggggCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACgcgggc                                             <  1:470914/101‑1 (MQ=255)
                                                    gggCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACgcgggcg                                            >  1:650519/1‑101 (MQ=255)
                                                       cgcgGATCCTCTCGACCGGGGCGGCCGGGGAGAGCCCGCCGGGGGTGGTGCTTCTGGCGCTTGACAGCGACGATCGGGCGATCGGGGCAACGGGGgcggcg                                         >  1:743175/1‑101 (MQ=255)
                                                       cgcgGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGgcggcg                                         >  1:691184/1‑101 (MQ=255)
                                                        gcgGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGgcggcgc                                        >  2:1350053/1‑101 (MQ=255)
                                                          ggATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGgcggcgcgg                                      >  1:2533562/1‑101 (MQ=255)
                                                          ggATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGgcggcgcgg                                      >  2:4143498/1‑101 (MQ=255)
                                                           gATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGgcggcgcggc                                     >  2:1197929/1‑101 (MQ=255)
                                                             tCCTCTCGACGGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCggcccggcgc                                   >  2:1485104/1‑101 (MQ=255)
                                                              ccTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGcc                                  <  2:2580743/101‑1 (MQ=255)
                                                              ccTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGcc                                  >  1:3175910/1‑101 (MQ=255)
                                                               ctctCGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCa                                 <  1:1675797/101‑1 (MQ=255)
                                                                tctcGGCCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAg                                <  1:3303597/101‑1 (MQ=255)
                                                                tctcGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAg                                >  2:1477199/1‑101 (MQ=255)
                                                                 ctcGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGTTCTGGTGATCGCCGCGCCCCGGGCGGCGCGGCGCCacg                               >  2:3323792/1‑99 (MQ=255)
                                                                 ctcGACCGAAGCGGCCGGGGAGAGCCCGCAGGGGGTGGTGCTTCTGGAGCTTGACCGCGCCGATCTGGTGATCGGCGCGCCGCGGGCAGCGCGGCGCCCGc                               >  1:3149125/1‑101 (MQ=255)
                                                                  tcGGCCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCt                              <  2:3509983/101‑1 (MQ=255)
                                                                   cGACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTg                             >  1:620336/1‑101 (MQ=255)
                                                                    gACCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGGGATCGGCGCGACGCGGgcggcgggggcccggcagg                            >  2:909737/1‑91 (MQ=255)
                                                                    gACCGGAGCGGCCGAGGAGAACCCGCAGGGGATGGTGCTTCTGGCGCTAGACCGCGACGATCTGGTGATCGGCCGGACGCGGGCGGCGCGGCGCCAGCTgg                            <  1:3997635/101‑1 (MQ=255)
                                                                     aCCGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTggg                           <  1:3726193/101‑1 (MQ=255)
                                                                      ccGGAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGc                          >  2:1702416/1‑101 (MQ=255)
                                                                         gAGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTg                       <  1:2952509/101‑1 (MQ=255)
                                                                          aGCGGCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTgg                      <  1:3348702/101‑1 (MQ=255)
                                                                             ggCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTggagg                   >  1:1141449/1‑101 (MQ=255)
                                                                             ggCCGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTggagg                   <  2:1708804/101‑1 (MQ=255)
                                                                               ccGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGgaggag                 <  2:4585336/101‑1 (MQ=255)
                                                                               ccGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGgaggag                 >  2:4091890/1‑101 (MQ=255)
                                                                                cGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGaggagg                <  1:734396/101‑1 (MQ=255)
                                                                                cGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGaggagg                >  1:3549092/1‑101 (MQ=255)
                                                                                cGAGGAGAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGaggagg                >  1:3651915/1‑101 (MQ=255)
                                                                                  aggagAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGgcg              >  1:318501/1‑101 (MQ=255)
                                                                                  aggagAGCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGGGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGgcg              >  2:4438081/1‑101 (MQ=255)
                                                                                   ggagAGCCCGCAGGGGGTGGTGCTTCTGGCGCTGGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTCGAGGAGgcgc             <  1:4104173/101‑1 (MQ=255)
                                                                                     agagCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAg           <  2:451460/101‑1 (MQ=255)
                                                                                       ggcgcGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGAt         <  1:2001148/97‑1 (MQ=255)
                                                                                       agCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGAt         <  2:655113/101‑1 (MQ=255)
                                                                                       agCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGAt         <  1:1081079/101‑1 (MQ=255)
                                                                                       agCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGAt         >  1:3885472/1‑101 (MQ=255)
                                                                                       agCCCGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGAt         >  2:238880/1‑101 (MQ=255)
                                                                                         cccGCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATgg       <  1:2798247/101‑1 (MQ=255)
                                                                                            gCAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGcc    >  1:390473/1‑101 (MQ=255)
                                                                                             cAGGGGGTGGTGCTTCTGGCGCTTGACCGCGAGGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGCCg   <  2:3707575/101‑1 (MQ=255)
                                                                                             cAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGCCg   >  2:404855/1‑101 (MQ=255)
                                                                                             cAGGGGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGCCg   <  2:1106019/101‑1 (MQ=255)
                                                                                             cAGGGGGGGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGGGCAGATGGGCCg   >  2:2181910/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CAGGATGCGGCCAGCCGGATCGAGGCGGATCTGTTCCGCGCCTCCTTCGAGGGGGCGCGGATCCTCTCGACCGGAGCGGCCGAGGAGAGCCCGCAGGCGGTGGTGCTTCTGGCGCTTGACCGCGACGATCTGGTGATCGGCGCGACGCGGGCGGCGCGGCGCCAGCTGGGCCTGGAGGAGGCGCAGATGGGCCGG  >  NC_014034/1751288‑1751482

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG