Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,770,645 G→C 100% R326P (CGG→CCG)  lpxB → lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,770,6450G→C100.0% 753.3 198R326P (CGG→CCG) lpxBlipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  new base (104/94):  ref base (0/0):  total (104/94)

Only 100 of 211 total aligned reads displayed.

GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770550‑1770741
                                                                                               |                                                                                                
 aCGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTg                                                                                            <  2:1787210/101‑1 (MQ=255)
  cGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGt                                                                                           >  1:1579421/1‑101 (MQ=255)
  cGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGACAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGt                                                                                           >  1:4699420/1‑101 (MQ=255)
   gTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTc                                                                                          <  1:1386526/101‑1 (MQ=255)
    tGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCg                                                                                         >  1:1491637/1‑101 (MQ=255)
    tGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCg                                                                                         >  2:4412388/1‑101 (MQ=255)
    tGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCg                                                                                         >  2:2661268/1‑101 (MQ=255)
       cATATCACGAAGCGCGCGATGCTGATAGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGcc                                                                                      >  2:1843685/1‑101 (MQ=255)
         tatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGa                                                                                    >  1:3841494/1‑101 (MQ=255)
          atCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaa                                                                                   <  1:992667/101‑1 (MQ=255)
          atCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaa                                                                                   >  2:3142662/1‑101 (MQ=255)
           tCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaaa                                                                                  <  2:2048012/101‑1 (MQ=255)
           tCAAGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGCGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaaa                                                                                  >  2:1408117/1‑101 (MQ=255)
            cACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAc                                                                                 <  2:3379629/101‑1 (MQ=255)
            cACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAc                                                                                 <  1:1082618/101‑1 (MQ=255)
             aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                                <  1:1940937/101‑1 (MQ=255)
             aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                                >  1:2816619/1‑101 (MQ=255)
              cGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGTCCGGCCTGTCGGCCGAAACCg                                                                               <  1:3503215/101‑1 (MQ=255)
              cGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCg                                                                               <  1:100956/101‑1 (MQ=255)
               gAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGGAACCGa                                                                              >  2:670687/1‑101 (MQ=255)
                 aGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATg                                                                            <  2:3043941/101‑1 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                       >  2:2716002/1‑101 (MQ=255)
                        gATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCggg                                                                     <  1:708771/101‑1 (MQ=255)
                        gATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCggg                                                                     >  1:4292703/1‑101 (MQ=255)
                        gATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCggg                                                                     >  2:1952730/1‑101 (MQ=255)
                          tGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcg                                                                   >  2:832075/1‑101 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 >  1:3030416/1‑101 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 <  2:1444937/101‑1 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                 <  2:2436775/101‑1 (MQ=255)
                              gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGc                                                               >  2:2202908/1‑101 (MQ=255)
                                 cGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAgg                                                            >  2:4591082/1‑101 (MQ=255)
                                  gACACGGTGTCGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGa                                                           >  2:1261712/1‑101 (MQ=255)
                                  gACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGa                                                           >  2:727061/1‑101 (MQ=255)
                                   acacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAt                                                          <  2:954600/101‑1 (MQ=255)
                                   acacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAt                                                          >  1:3335392/1‑101 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                        <  2:1579321/101‑1 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                        >  2:3955983/1‑101 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                        >  2:3734634/1‑101 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                        >  2:2406103/1‑101 (MQ=255)
                                       ggTGACGCTTGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                      <  2:1353609/101‑1 (MQ=255)
                                       ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                      <  2:280921/101‑1 (MQ=255)
                                         tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                    >  1:1119915/1‑101 (MQ=255)
                                         tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                    <  2:43552/101‑1 (MQ=255)
                                          gACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGc                                                   >  1:1138904/1‑101 (MQ=255)
                                           aCGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCg                                                  <  2:4628044/101‑1 (MQ=255)
                                              cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                               >  1:3039626/1‑101 (MQ=255)
                                              cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                               >  1:2359212/1‑101 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGAt                                              >  2:3465338/1‑101 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGAt                                              >  1:2942522/1‑101 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             >  1:2292392/1‑101 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             >  2:4197603/1‑101 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             <  2:1718314/101‑1 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                             >  1:129891/1‑101 (MQ=255)
                                                 gTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATcc                                            <  1:4125623/101‑1 (MQ=255)
                                                   cAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGc                                          >  2:1130728/1‑101 (MQ=255)
                                                   cAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGc                                          <  1:763634/101‑1 (MQ=255)
                                                   cAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGc                                          >  1:2910077/1‑101 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCa                                         >  1:2504457/1‑101 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCa                                         >  1:4265/1‑101 (MQ=255)
                                                     aTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCaa                                        <  2:4547583/101‑1 (MQ=255)
                                                      tCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAc                                       <  2:824464/101‑1 (MQ=255)
                                                      tCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAc                                       <  1:4362657/101‑1 (MQ=255)
                                                       cTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcg                                      <  1:3603139/101‑1 (MQ=255)
                                                         ggTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgcc                                    <  2:1047758/101‑1 (MQ=255)
                                                         ggTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCCGTCGGCCGAAACCCATGGCGGGGGGGCCGCCGGATCTCTTGCGGGGTCCGCAAcccc                                    >  2:3333419/1‑101 (MQ=255)
                                                           tgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccgc                                  >  1:370081/1‑101 (MQ=255)
                                                           tgcgtGACACCCGGGCGGTCCCGAAATTCTTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccgc                                  <  2:4231004/97‑1 (MQ=255)
                                                              cTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcgcg                               <  2:2469559/101‑1 (MQ=255)
                                                               tGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcgg                              <  2:390148/101‑1 (MQ=255)
                                                                   aCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGGAGGATCTGTTGCGGGATCCGCAACGCCGCGAGGCGCa                          >  1:4025385/1‑101 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCGACCCCGCGCGGCGCAg                         >  1:442773/1‑101 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                         <  1:239479/101‑1 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                         <  1:3725459/101‑1 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                         <  1:4532971/101‑1 (MQ=255)
                                                                      cGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGt                       >  1:637003/1‑101 (MQ=255)
                                                                      cGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGt                       >  1:1380815/1‑101 (MQ=255)
                                                                       gggCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGtt                      <  2:198910/101‑1 (MQ=255)
                                                                       gggCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGtt                      <  1:2654801/101‑1 (MQ=255)
                                                                         gCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTc                    <  2:139103/101‑1 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                  >  1:794163/1‑101 (MQ=255)
                                                                               ccGGAATTCCTCGGCCCGGCCTGCCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCAt              <  2:4025143/101‑1 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGGGCAGGTTTCCGCCATgg            >  2:4737720/1‑101 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTCCCGCCATgg            <  2:442729/101‑1 (MQ=255)
                                                                                  gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGa           >  2:3565360/1‑101 (MQ=255)
                                                                                  gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGa           >  1:2070809/1‑101 (MQ=255)
                                                                                  gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGa           >  1:4153624/1‑101 (MQ=255)
                                                                                  gAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAAGCCGCGCGGCGCAGGTTTCCGCCATGGa           >  1:4347024/1‑101 (MQ=255)
                                                                                   aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          >  2:557617/1‑101 (MQ=255)
                                                                                   aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa          <  2:4178072/101‑1 (MQ=255)
                                                                                    aTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaaa         >  2:367731/1‑101 (MQ=255)
                                                                                    aTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaaa         <  1:1413052/101‑1 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAac        <  1:1722396/101‑1 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGGTTCCGCCATGGAAacg       >  2:2441760/1‑101 (MQ=255)
                                                                                       ccTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacga      <  1:594995/101‑1 (MQ=255)
                                                                                       ccTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacga      <  1:2481162/101‑1 (MQ=255)
                                                                                       ccTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacga      <  2:2359079/101‑1 (MQ=255)
                                                                                         tCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgacg    >  2:4637264/1‑101 (MQ=255)
                                                                                           ggcccggcCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAt  >  2:3390504/1‑101 (MQ=255)
                                                                                           ggcccggcCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAt  <  1:2441482/101‑1 (MQ=255)
                                                                                           ggcccggcCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAt  <  2:2384487/101‑1 (MQ=255)
                                                                                               |                                                                                                
GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGAT  >  NC_014034/1770550‑1770741

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG