Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,184 T→A 75.7% S661T (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1840T→A75.7% 155.6 ‑3.7 0.0181S661T (TCA→ACA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (57/80):  ref base (27/17):  total (84/97)
Fisher's exact test strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275
                                                                                          |                                                                                            
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  1:1498276/1‑101 (MQ=255)
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  1:3574487/1‑101 (MQ=255)
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGGGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  2:1339226/1‑101 (MQ=255)
 gtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  >  1:2221042/1‑101 (MQ=255)
  tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaa                                                                                 >  2:3814265/1‑101 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  1:611718/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  2:2728139/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  2:1028290/101‑1 (MQ=255)
      gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGc                                                                             <  2:691056/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCg                                                                           >  1:3322843/1‑101 (MQ=255)
         tgcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCGTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGc                                                                          <  2:153108/101‑1 (MQ=255)
          gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCt                                                                         >  2:745843/1‑101 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtg                                                                      >  1:1647504/1‑101 (MQ=255)
              tgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtgt                                                                     <  2:3287531/101‑1 (MQ=255)
              tgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtgt                                                                     >  2:2152547/1‑101 (MQ=255)
               gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtgtg                                                                    <  1:1684868/101‑1 (MQ=255)
               gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.GTCCATGACGCCAAAAGCCGCtgtgtg                                                                    >  2:1436297/1‑101 (MQ=255)
                 gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGcc                                                                  >  2:4354523/1‑101 (MQ=255)
                 gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGcc                                                                  >  1:1015399/1‑101 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  2:824264/101‑1 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  2:2127161/101‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  1:3260475/101‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCtt                                                                >  1:1996791/1‑101 (MQ=255)
                    tATGGCGCGATCAAGCTCCCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  2:1576607/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  2:4166635/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               >  1:3101763/1‑101 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  2:3574268/101‑1 (MQ=255)
                      tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             <  2:686352/101‑1 (MQ=255)
                       ggCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGt                                                            <  1:2961328/101‑1 (MQ=255)
                         cgcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGc                                                          <  1:1281585/101‑1 (MQ=255)
                           cgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTg                                                        <  2:2055206/101‑1 (MQ=255)
                             aTCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggc                                                      <  1:3347684/101‑1 (MQ=255)
                             aTCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggc                                                      <  1:3478941/101‑1 (MQ=255)
                                aaGGGCAACGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggaggc                                                   >  2:973521/1‑101 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  2:1230685/101‑1 (MQ=255)
                                 aGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGgcggcg                                                  <  2:3971723/101‑1 (MQ=255)
                                   cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  2:3746742/101‑1 (MQ=255)
                                   cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               >  2:1891929/1‑101 (MQ=255)
                                   cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  2:4177909/101‑1 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  1:2201507/101‑3 (MQ=255)
                                      aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            >  2:4113250/1‑98 (MQ=255)
                                       ccGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacga                                           >  1:4635817/1‑97 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        <  2:3504560/101‑1 (MQ=255)
                                            gagaTCCCCTATTCCGAGTGTTTCCAGGGCCTTGACTGGGTGGCG.AACCATGACGACAAAAGCCGCTGGGGTCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       <  2:4235213/101‑1 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      >  2:250209/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      >  2:4200768/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGCTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGGGCTGGCGGCGAAACGAAAGGCt                                      >  1:86512/1‑101 (MQ=255)
                                              gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     <  1:430392/101‑1 (MQ=255)
                                              gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     <  2:1500835/101‑1 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    <  2:3101563/101‑1 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    >  1:400704/1‑101 (MQ=255)
                                                  cGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 <  2:707203/101‑1 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCa                                >  1:2118955/1‑101 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCa                                >  1:1046162/1‑101 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCa                                <  1:303620/101‑1 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               <  2:3848104/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:403812/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:885153/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:1673450/101‑1 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  1:184905/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  1:1745300/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            >  2:4443092/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            >  1:721081/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:3634047/101‑1 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  2:3761152/101‑1 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           >  1:2619425/1‑101 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           <  2:4506862/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACCAAAGCCGGTGTGTGCCTTTGCTGCTGGCGGGGAAACGACAAGCTCGGACATGAAgg                          >  1:3935492/1‑100 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          <  1:2782180/101‑1 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  2:3272081/1‑101 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      >  2:4631955/1‑101 (MQ=255)
                                                              tgtTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcgg                     >  1:3702774/1‑101 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGGCGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    <  1:4113492/101‑1 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    >  1:689929/1‑101 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    >  1:2114885/1‑101 (MQ=255)
                                                                tttCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggcg                   >  2:2645024/1‑101 (MQ=255)
                                                                tttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggcg                   <  1:2152671/101‑1 (MQ=255)
                                                                 ttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  >  2:2392468/1‑101 (MQ=255)
                                                                 ttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  >  1:2060352/1‑101 (MQ=255)
                                                                 tgccAGTGCCTTGACTGTGGGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  <  2:1140204/99‑1 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGt                 >  1:3074417/1‑101 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGt                 >  2:539704/1‑101 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               <  1:1144978/101‑1 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              <  1:4414680/101‑1 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              <  2:1586857/101‑1 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            >  1:4228120/1‑101 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            <  2:1858911/101‑1 (MQ=255)
                                                                        gCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           >  1:3748341/1‑101 (MQ=255)
                                                                        gCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGc           >  2:3704735/1‑101 (MQ=255)
                                                                        gCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           <  1:4173623/101‑1 (MQ=255)
                                                                        gCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           <  2:1775920/101‑1 (MQ=255)
                                                                        gCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           <  2:2623254/101‑1 (MQ=255)
                                                                         ccTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCa          >  2:3056829/1‑101 (MQ=255)
                                                                           ttGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        >  1:4429898/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:2473488/1‑101 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    <  1:1892041/101‑1 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    <  2:1647404/101‑1 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    >  1:1584874/1‑101 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   >  1:2704643/1‑101 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTGTCTGCAGATCGTgg  >  2:493377/1‑101 (MQ=255)
                                                                                          |                                                                                            
TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG