Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,951,208 G→C 92.1% A129G (GCG→GGG)  ssb1 ← single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,951,2080G→C92.1% 26.9 ‑0.9 ‑0.0164A129G (GCG→GGG) ssb1single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  new base (81/70):  ref base (5/8):  total (86/78)
Fisher's exact test strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.31e-01

Only 100 of 175 total aligned reads displayed.

GCCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299
                                                                                              |                                                                                            
gccggcggggggggCCGAGGAGCCGCCGCCCCCGCCGCCATAGCCCGGCCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAAt                                                                                       <  2:4108352/92‑1 (MQ=255)
 cccGGCGGGGGGGCCGAGGAGCCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAAtt                                                                                      <  2:4557520/101‑1 (MQ=255)
 cccGAGGGGGGGGCGGAGGAGCCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAAtt                                                                                      <  1:4437601/101‑1 (MQ=255)
 cccGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAAtt                                                                                      >  2:818583/1‑101 (MQ=255)
  ccGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTg                                                                                     >  1:3601333/1‑101 (MQ=255)
   cgacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAAtctc                                                                                    >  2:742549/1‑98 (MQ=255)
    gcggggggggCGGGGGGGCCGCCGCCCCCGCCGCCATGGCCGGGGCCGCCATGGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGcc                                                                                   <  1:3537284/96‑1 (MQ=255)
    gacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTACTTGcc                                                                                   >  1:3150453/1‑101 (MQ=255)
    gacgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGcc                                                                                   >  1:1827623/1‑101 (MQ=255)
    atctggggggCGGAGGACCCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGcc                                                                                   <  1:3703241/96‑1 (MQ=255)
     acgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCa                                                                                  >  1:2425907/1‑101 (MQ=255)
     acgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCCTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCa                                                                                  >  2:3042411/1‑101 (MQ=255)
      ggggggggCGGGGGCCCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAc                                                                                 <  2:1596150/98‑1 (MQ=255)
      cggggnngCCGGGGGGCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAc                                                                                 <  1:2329343/98‑1 (MQ=255)
      cgaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCACCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAc                                                                                 >  1:4335410/1‑101 (MQ=255)
       gggggggCCGGGGAGCCGCCCCCCCCCCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCAcc                                                                                <  1:2757510/99‑1 (MQ=255)
       gaGGGGGCGGGGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCAcc                                                                                <  1:837630/101‑1 (MQ=255)
       gaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAcc                                                                                >  1:680561/1‑101 (MQ=255)
       gaGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAcc                                                                                >  1:1910358/1‑101 (MQ=255)
        aGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCagat                                                                               >  2:3703011/1‑98 (MQ=255)
         gggggCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAccgc                                                                              >  2:1483701/1‑101 (MQ=255)
           gggCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCAccgccg                                                                            >  1:1599928/1‑101 (MQ=255)
            ggcgggggccccgccccccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACcgccgc                                                                           <  1:4395017/91‑1 (MQ=255)
                gaggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGa                                                                       >  1:1784135/1‑101 (MQ=255)
                  ggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAgc                                                                     >  1:4450146/1‑101 (MQ=255)
                     gccgccgccACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAgccgc                                                                  >  1:2922738/1‑101 (MQ=255)
                       cgccgcccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAgccgccg                                                                <  1:3075939/101‑1 (MQ=255)
                       ccccccccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAgccgccg                                                                <  1:1156341/93‑1 (MQ=255)
                        cccccccccgccgccaTAGCCCGGCCCGTCAGCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAgccgccgc                                                               <  2:3661582/94‑1 (MQ=255)
                            cccccgccgccaTGGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           <  1:4274151/98‑1 (MQ=255)
                            ccccccccgccaTGCCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           <  2:2284770/95‑1 (MQ=255)
                            ccccccccgccaCACCCCGGGCCGCCCTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           <  1:4540296/95‑1 (MQ=255)
                            ccaccgccgccaTAGCCGGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           >  2:18328/1‑101 (MQ=255)
                            ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           >  1:4086368/1‑101 (MQ=255)
                              cccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                         <  1:528099/100‑1 (MQ=255)
                               ccccccccaTAGCCCGGGCCGTCAGCGTAGCCGCCGCCATAGCCGCCGCCCCGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                        <  1:158454/95‑1 (MQ=255)
                                 gccgccaTGGCCCGGGCCGCCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                      <  1:2539980/101‑1 (MQ=255)
                                  ccgccaTCCCCGGGGCCCTCATCGTGGCCGCAGCCATAGCCGCCGCCCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     <  1:1710421/101‑1 (MQ=255)
                                  ccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCCTCCCGgcc                                                     >  2:1063906/1‑101 (MQ=255)
                                  ccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     <  2:1261174/101‑1 (MQ=255)
                                    gccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                   >  1:1324637/1‑101 (MQ=255)
                                      caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                 >  1:1308966/1‑101 (MQ=255)
                                      caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                 >  2:346812/1‑101 (MQ=255)
                                      caTACCCCGGGCCGTCACCGTAGCCGCCGCCATTGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                 <  1:4067934/101‑1 (MQ=255)
                                       cgggCCCGGGCCCTCTTCTTGCCCCCCCCCATACCCGCCGCCCTGCGAGCCGCCGC.CGTAATTCCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                <  1:3789895/98‑1 (MQ=255)
                                       aTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                >  2:155512/1‑101 (MQ=255)
                                       aTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                >  2:2408926/1‑101 (MQ=255)
                                       aTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                >  2:2887470/1‑101 (MQ=255)
                                         aGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                              >  2:3929870/1‑101 (MQ=255)
                                         aGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                              <  1:2675952/101‑1 (MQ=255)
                                            cgtgcccGTCATCGAAGCCGCCGCCAAAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcagc                                           <  2:3161890/96‑1 (MQ=255)
                                            ccGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcagc                                           >  1:349640/1‑101 (MQ=255)
                                               ggcCGTCATCGTGGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  1:1505091/100‑1 (MQ=255)
                                               ggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        >  1:84523/2‑101 (MQ=255)
                                               ggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        >  1:1137366/2‑101 (MQ=255)
                                               gcccGTCTCCGTGGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  2:4510415/99‑1 (MQ=255)
                                               gcccGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  1:818656/99‑1 (MQ=255)
                                                   gTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGt                                    >  2:763796/1‑101 (MQ=255)
                                                     caccGTGGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTg                                  >  2:4613357/4‑101 (MQ=255)
                                                      aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                 >  2:3615163/1‑101 (MQ=255)
                                                      aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                 >  2:3271062/1‑101 (MQ=255)
                                                      aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                 >  2:3938914/1‑101 (MQ=255)
                                                        cgcggccgccgccCTCGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               <  1:1297809/97‑1 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccag                              >  2:2127069/1‑99 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                              >  2:4754250/1‑101 (MQ=255)
                                                          tacccgccgccATAGCCGCCGCCCTGCGACCCCCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                             <  2:2604797/98‑1 (MQ=255)
                                                          tAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                             >  1:3257834/1‑101 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCTTCAGGTTGCCCgca                            >  1:4700006/1‑99 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            >  2:1329189/1‑101 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            >  1:1892684/1‑101 (MQ=255)
                                                            gccgccgccATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCTCAAGGTTGCCCGtat                           >  1:4183815/1‑101 (MQ=255)
                                                              ccccgccATAGCCGCCGCCCGGCGACTCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAg                         <  1:2854515/99‑1 (MQ=255)
                                                               gccgccATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                        >  2:866479/1‑101 (MQ=255)
                                                                ccgccATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAggg                       >  2:2749179/1‑101 (MQ=255)
                                                                cccccATAGCCGCCGCCCGGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAggg                       <  2:4330765/98‑1 (MQ=255)
                                                                  gccATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgc                     >  1:4123220/1‑101 (MQ=255)
                                                                    cATAGCCGCCGCCCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   <  2:4447902/101‑1 (MQ=255)
                                                                    cATAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   >  2:704692/1‑101 (MQ=255)
                                                                    cATAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   >  2:208869/1‑101 (MQ=255)
                                                                     aTAGGCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                  >  2:4018386/1‑101 (MQ=255)
                                                                     aTAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                  >  2:639920/1‑101 (MQ=255)
                                                                      tAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcag                 <  1:2596718/101‑1 (MQ=255)
                                                                      tAGCCGCCGCCCTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcag                 >  1:3904265/1‑101 (MQ=255)
                                                                      tAGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcag                 >  2:365368/1‑101 (MQ=255)
                                                                       aGCCGCCGCCCTGCGAACCGC.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                >  1:3705842/1‑101 (MQ=255)
                                                                       aGCCGCCGCCCGGCGAACCGCCGC.CGTAATTGCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                <  2:1224247/101‑1 (MQ=255)
                                                                       aGCCGCCCCCCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                <  1:2087515/101‑1 (MQ=255)
                                                                          cgccgccCTGCGAACCGCCGC.CGAAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc             <  2:1910246/101‑1 (MQ=255)
                                                                          cgcccccCTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc             <  1:3356605/101‑1 (MQ=255)
                                                                           cccgccCGGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacca            <  1:192087/100‑1 (MQ=255)
                                                                            ccgccCGGCGACCCG.GCGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcaccac           <  1:3934951/101‑1 (MQ=255)
                                                                              ccccTGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt         <  2:2481589/100‑1 (MQ=255)
                                                                              ccccGGCGACCCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt         <  2:1968579/100‑1 (MQ=255)
                                                                                ccTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg       <  1:982741/101‑1 (MQ=255)
                                                                                ccTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg       >  1:3042794/1‑101 (MQ=255)
                                                                                 cTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgg      >  1:545816/1‑101 (MQ=255)
                                                                                 cTGCGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgg      >  2:2026100/1‑101 (MQ=255)
                                                                                    cGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg   >  1:1129243/1‑101 (MQ=255)
                                                                                    cGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg   >  2:2193404/1‑101 (MQ=255)
                                                                                    cGAACCGCCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg   >  2:3781834/1‑101 (MQ=255)
                                                                                              |                                                                                            
GCCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG