Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,239	G→T	100%	intergenic (+7/+85)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,239	0	G→T	100.0%	234.1	61	intergenic (+7/+85)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (32/29):  ref base (0/0):  total (32/29)

TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.GCGGGCTC.CG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAG  >  NC_014034/1965141‑1965327                                                                                                   |                                                                                             tGGGGGGAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcg                                                                                            <  1:1874151/101‑1 (MQ=255) tGGGGGGAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcg                                                                                            <  2:2319861/101‑1 (MQ=255) tGGGGGGAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcg                                                                                            <  1:4326068/101‑1 (MQ=255)  ggggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcgc                                                                                           <  2:4270662/101‑1 (MQ=255)   gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGcc                                                                                          >  1:2031487/1‑101 (MQ=255)    ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCt                                                                                         <  2:4353506/101‑1 (MQ=255)    ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTAGCCt                                                                                         >  1:891453/1‑101 (MQ=255)     gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                        <  2:750752/101‑1 (MQ=255)     gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                        <  2:2705463/101‑1 (MQ=255)     gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                        <  1:4421701/101‑1 (MQ=255)      ggAACGATGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                       <  1:2292513/101‑1 (MQ=255)      ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                       <  2:488538/101‑1 (MQ=255)       gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGGCGGCGCTGTGAGAGATTTCGCCTCgg                                                                                      >  1:4393118/1‑101 (MQ=255)         acgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgggg                                                                                    <  2:1884791/101‑1 (MQ=255)         acgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgggg                                                                                    <  1:4514460/101‑1 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGGGCTGGGAGAGATTTCGCCTCGGGGc                                                                                   >  2:2420722/1‑101 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                   >  2:4202703/1‑101 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                   >  2:4414520/1‑101 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                   <  1:3795715/101‑1 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                   <  1:2836900/101‑1 (MQ=255)           gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCa                                                                                  >  2:2715220/1‑101 (MQ=255)           gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCa                                                                                  <  2:4424304/101‑1 (MQ=255)            acgTTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCaa                                                                                 <  1:4255356/101‑1 (MQ=255)            acgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCaa                                                                                 >  1:2319357/1‑101 (MQ=255)             cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                >  2:2150231/1‑101 (MQ=255)             cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                >  1:1411442/1‑101 (MQ=255)              ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                               >  1:2920257/1‑101 (MQ=255)              ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                               >  2:1160822/1‑101 (MQ=255)               gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGcc                                                                              <  2:2031370/101‑1 (MQ=255)                tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                             <  1:3598090/101‑1 (MQ=255)                tCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                             <  2:3103978/101‑1 (MQ=255)                 cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                            <  1:3585580/101‑1 (MQ=255)                 cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                            >  1:4562534/1‑101 (MQ=255)                 cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                            >  2:1002360/1‑101 (MQ=255)                  ttCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGa                                                                           <  1:3930988/101‑1 (MQ=255)                  ttCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGa                                                                           <  1:2341935/101‑1 (MQ=255)                   tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCggg                                                                          >  1:4291125/1‑99 (MQ=255)                   tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                          <  1:3460074/101‑1 (MQ=255)                   tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                          <  2:4562281/101‑1 (MQ=255)                    ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                         >  2:1343648/1‑101 (MQ=255)                    ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                         >  1:3404094/1‑101 (MQ=255)                      tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                       <  1:2640337/101‑1 (MQ=255)                      tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                       >  1:2791272/1‑101 (MQ=255)                      tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                       <  1:423302/101‑1 (MQ=255)                      tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                       <  1:317691/101‑1 (MQ=255)                       gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGCCGATTTCTGTGGATGGCGGCTGGGCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                      >  1:3949131/1‑101 (MQ=255)                       gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                      >  2:4256854/1‑101 (MQ=255)                       gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                      >  1:1158823/1‑101 (MQ=255)                       gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGa                                                                      >  2:3057838/1‑101 (MQ=255)                         gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                    <  1:546684/98‑1 (MQ=255)                         gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                    <  1:4336132/98‑1 (MQ=255)                         gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCCAGGGGAcc                                                                    >  2:4042737/4‑101 (MQ=255)                          ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGGAAGCCCGAGGCGAccc                                                                   >  1:764680/3‑101 (MQ=255)                          ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                   <  2:3948889/99‑1 (MQ=255)                          ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                   <  2:672238/99‑1 (MQ=255)                           tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg                                                                  <  1:2827179/100‑1 (MQ=255)                           tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg                                                                  <  1:4487666/100‑1 (MQ=255)                             cGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccggc                                                                >  1:1149703/1‑99 (MQ=255)                              gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.ccc                                                              >  1:2820884/1‑100 (MQ=255)                              gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cc.g                                                             >  2:283593/1‑101 (MQ=255)                               aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cccg                                                             >  1:2087111/1‑99 (MQ=255)                               aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cccg                                                             >  2:3525663/1‑99 (MQ=255)                               aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cc.gg                                                            >  2:553744/1‑100 (MQ=255)                                 gcggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cccggg                                                           >  1:425807/1‑97 (MQ=255)                                   ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cccggggg                                                         >  1:3492976/1‑95 (MQ=255)                                          cAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.CCggctggccgtt                                                 >  1:3827920/1‑92 (MQ=38)                                           aGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAGCCG.CC.GCGGGGGC.CG.cccc                                              >  2:718153/1‑101 (MQ=39)                                             aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC.GCggggggggccccc                                               >  1:1027284/1‑91 (MQ=38)                                             aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.GGGGGGGG.CG.cccccc                                            >  2:2817090/1‑99 (MQ=37)                                                     cACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.GGGGGGGC.CC.cccccccgcccccc                                    >  2:4430244/1‑91 (MQ=25)                                                         acgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGCGC.CC.CCCCCGCccccccccgg                                 >  2:4631117/1‑96 (MQ=21)                                                             tgtCTGTGGGGGGGGGCGGGCCGGCGCTGTGAGATTTTCCGCCGCGGGGCAACCCCGGGGCGCCCCG.CC.GGGGGCTC.CGCCCCCCGACCCCCGAGGTAttt                             <  1:4147859/99‑1 (MQ=12)                                                                gtggggggggcggcGGGGCGGCGCGGTGAGAGTTTTCCCCTGGGGGCACCCCGGGGGCCCCCCG.CC.GGGGGCTC.CGCCCCCGGACCCCCGAGGTATTTCAg                          <  2:530586/93‑1 (MQ=9)                                                                          cggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.GGGGGGTC.CGCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGaa                >  2:2331316/1‑101 (MQ=21)                                                                                   gggcgtgggagagtTTGCGCCCGGGGGCACGCCCGGGGCCCCCCG.CC.GGGGGCTC.CGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGgcg       <  1:673868/93‑1 (MQ=16)                                                                                   ggCGGTGTGGGAGTTTGCGCCGGGGGCCACCCCCGGGGCGCCCCG.CC.GGGGGCTC.CGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGgcg       <  1:2150355/101‑1 (MQ=12)                                                                                         gtgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC.GGGGGCCCGCC.CCCCCGCCCCCCCCGGGGTTTCGGGCAAAGGGAAAGAGGGgggccccc  >  1:2093022/1‑97 (MQ=2)                                                                                                   |                                                                                             TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC.GCGGGCTC.CG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAG  >  NC_014034/1965141‑1965327 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG