BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,990,962	T→A	100%	Y555F (TAC→TTC)	glgB ←	1,4‑alpha‑glucan branching protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,990,962	0	T→A	100.0%	989.3	254	Y555F (TAC→TTC)	glgB	1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand): new base (116/138): ref base (0/0): total (116/138)

Only 100 of 257 total aligned reads displayed.


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059


gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 >  1:396408/1‑101 (MQ=255)


 aaGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACg                                                                                                <  2:4546307/101‑1 (MQ=255)


  aGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGc                                                                                               <  2:3650524/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             >  2:1941650/1‑101 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  2:2494237/101‑1 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           >  1:490989/1‑101 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           >  2:2535552/1‑101 (MQ=255)


       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  2:4433673/101‑1 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCTCCCCTGCGCTTACTCCTTCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  1:1529520/101‑1 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  2:191533/101‑1 (MQ=255)


          gTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAgg                                                                                       >  2:611648/1‑101 (MQ=255)


           tCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGc                                                                                      >  1:3414703/1‑101 (MQ=255)


           tCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGc                                                                                      >  2:2376068/1‑101 (MQ=255)


           tCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGc                                                                                      >  2:3710800/1‑101 (MQ=255)


            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                     >  1:2423501/1‑101 (MQ=255)


            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                     >  2:93212/1‑101 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    <  2:4407629/101‑1 (MQ=255)


              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                   >  1:1502230/1‑101 (MQ=255)


              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                   >  2:3724975/1‑101 (MQ=255)


               gggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGc                                                                                  <  1:4193491/101‑1 (MQ=255)


               gggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGc                                                                                  <  1:1058708/101‑1 (MQ=255)


                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                <  2:119688/101‑1 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              <  1:3545929/101‑1 (MQ=255)


                    cccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGtt                                                                             <  1:4041183/101‑1 (MQ=255)


                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                            <  2:530359/101‑1 (MQ=255)


                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                            <  1:562854/101‑1 (MQ=255)


                      cTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTgg                                                                           <  1:3736926/101‑1 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:676992/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  1:2761415/1‑101 (MQ=255)


                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  1:1974458/1‑101 (MQ=255)


                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                        >  2:1988624/1‑101 (MQ=255)


                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                        >  2:25992/1‑101 (MQ=255)


                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                        <  1:3711206/101‑1 (MQ=255)


                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                        >  1:2316790/1‑101 (MQ=255)


                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                        >  1:4286555/1‑101 (MQ=255)


                            cgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAtt                                                                     <  2:890333/101‑1 (MQ=255)


                            cgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAtt                                                                     <  2:1011903/101‑1 (MQ=255)


                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                    <  2:2993276/101‑1 (MQ=255)


                               ccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                  <  2:2056247/101‑1 (MQ=255)


                                cATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTg                                                                 >  2:1507962/1‑101 (MQ=255)


                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGACGGCCCGCAGGTTGGCGAATTTCTGc                                                                >  2:3803456/1‑101 (MQ=255)


                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGc                                                                >  1:181670/1‑101 (MQ=255)


                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGc                                                                >  1:2370681/1‑101 (MQ=255)


                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               >  1:1802252/1‑101 (MQ=255)


                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               <  1:3874683/101‑1 (MQ=255)


                                   tCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCaa                                                              >  2:1442262/1‑101 (MQ=255)


                                   tCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCaa                                                              <  1:844472/101‑1 (MQ=255)


                                    cgcgCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAc                                                             <  2:230962/101‑1 (MQ=255)


                                    cgcgCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAc                                                             >  2:132835/1‑101 (MQ=255)


                                           cTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtctc                                                      <  1:421382/101‑1 (MQ=255)


                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    <  1:896046/101‑1 (MQ=255)


                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    >  1:4698896/1‑101 (MQ=255)


                                               gcgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCgg                                                  >  2:4165038/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               >  1:4578783/1‑101 (MQ=255)


                                                   aCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATc                                              <  2:3878864/101‑1 (MQ=255)


                                                    cTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCt                                             >  2:2980109/1‑101 (MQ=255)


                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCtt                                            <  2:3110859/101‑1 (MQ=255)


                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCtt                                            <  1:3084455/101‑1 (MQ=255)


                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCACATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTg                                           <  2:3132692/101‑1 (MQ=255)


                                                       cTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGc                                          <  1:1285432/101‑1 (MQ=255)


                                                        tGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGcc                                         >  2:2108816/1‑101 (MQ=255)


                                                          ccccATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCa                                       >  2:4062807/1‑101 (MQ=255)


                                                          ccccATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTAGCGAATTTCTGCAACCCGTCTCCAGGCATCTTGCCCa                                       >  2:4655141/1‑101 (MQ=255)


                                                            ncANGNNGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGc                                     <  1:1731055/100‑1 (MQ=255)


                                                             cATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCa                                    <  1:3104155/101‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  2:2640901/101‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  2:2824807/101‑1 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCg                                 >  1:2661858/1‑101 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCg                                 >  2:3303141/1‑101 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCg                                 <  2:3222226/101‑1 (MQ=255)


                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                >  1:2131566/1‑101 (MQ=255)


                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               <  1:1361802/101‑1 (MQ=255)


                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               >  1:2013167/1‑101 (MQ=255)


                                                                   gagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcc                              <  2:4076460/101‑1 (MQ=255)


                                                                     gcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcccc                            <  2:3754306/101‑1 (MQ=255)


                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          <  1:4550034/101‑1 (MQ=255)


                                                                       agcTTCTTGACCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          <  1:3142916/101‑1 (MQ=255)


                                                                         cttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGc                        >  2:161565/1‑101 (MQ=255)


                                                                          ttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGcc                       >  1:1128291/1‑101 (MQ=255)


                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      >  2:4741322/1‑101 (MQ=255)


                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      <  1:3013157/101‑1 (MQ=255)


                                                                             ttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTg                    >  1:4408092/1‑101 (MQ=255)


                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  1:1211445/101‑1 (MQ=255)


                                                                                 ccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACg                <  2:2757376/101‑1 (MQ=255)


                                                                                  cGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGa               <  1:1658672/101‑1 (MQ=255)


                                                                                   ggATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAc              <  1:3852287/101‑1 (MQ=255)


                                                                                    gATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAcc             >  2:3536812/1‑101 (MQ=255)


                                                                                    gATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAcc             >  1:4142335/1‑101 (MQ=255)


                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            <  1:3188500/101‑1 (MQ=255)


                                                                                      tGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCAAGCATCGACCACTTGCCGTGAACGACCtc           >  2:805763/1‑101 (MQ=255)


                                                                                      tGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCCCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtc           <  1:2535697/101‑1 (MQ=255)


                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcg          <  1:1593298/101‑1 (MQ=255)


                                                                                        cgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgt         <  2:4213876/101‑1 (MQ=255)


                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtc        <  2:1974341/101‑1 (MQ=255)


                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtc        >  2:4620812/1‑101 (MQ=255)


                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtc        >  2:304247/1‑101 (MQ=255)


                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGAAACACGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtc        >  2:1698807/1‑101 (MQ=255)


                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     >  2:4326193/1‑101 (MQ=255)


                                                                                             aTTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGa    <  1:3240231/101‑1 (MQ=255)


                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   >  1:1394598/1‑101 (MQ=255)


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG