Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,275 A→G 9.1% intergenic (+148/+29) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2750A→G 9.1% 22.6 ‑2.5 ‑0.0155intergenic (+148/+29)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (9/6):  ref base (115/25):  total (124/31)
Fisher's exact test strand distribution p-value = 8.09e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.51e-01

Only 100 of 200 total aligned reads displayed.

ATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAAGGGGGG.CGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCGA  >  NC_014034/2044178‑2044376
                                                                                                   |                                                                                                      
aTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGGGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGggg                                                                                                     >  1:185045/1‑101 (MQ=255)
 ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGgggg                                                                                                    >  1:4520318/1‑101 (MQ=255)
 ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGgggg                                                                                                    <  1:2539096/101‑1 (MQ=255)
  tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGggggg                                                                                                   >  1:1892177/1‑101 (MQ=255)
   cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGgggggc                                                                                                  >  2:2398426/1‑100 (MQ=255)
   cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAGgggggc                                                                                                  >  1:806864/1‑100 (MQ=255)
    gccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGg.cg                                                                                               <  2:4294555/100‑1 (MQ=255)
    aCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGg.cg                                                                                               <  2:4530036/101‑1 (MQ=255)
     ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGg.cgc                                                                                              >  1:2733528/1‑101 (MQ=255)
        ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGaa                                                                                           <  1:162070/101‑1 (MQ=255)
         cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAg                                                                                          <  2:3067742/101‑1 (MQ=255)
          ccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGc                                                                                         <  1:4224784/101‑1 (MQ=255)
             cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGcccc                                                                                      <  2:3811841/101‑1 (MQ=255)
              gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGccccc                                                                                     <  2:4704875/101‑1 (MQ=255)
              gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGccccc                                                                                     <  1:676130/101‑1 (MQ=255)
                  tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCGAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCttt                                                                                 >  2:2741956/1‑101 (MQ=255)
                  tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCttt                                                                                 >  1:144933/1‑101 (MQ=255)
                  tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCttt                                                                                 <  2:4434552/101‑1 (MQ=255)
                   atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttt                                                                                >  2:458293/1‑101 (MQ=255)
                   atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttt                                                                                >  2:3324751/1‑101 (MQ=255)
                   atTTGAGGCAAGATAAAGACTTGCGACGTGTTCTTCATCTTTCTACAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttt                                                                                <  2:4703122/101‑1 (MQ=255)
                    tttGAGGAAAGATAAAGCCTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCttttt                                                                               <  2:658436/101‑1 (MQ=255)
                     ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttttc                                                                              >  1:522927/1‑100 (MQ=255)
                      tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttttcc                                                                             >  1:1212873/1‑99 (MQ=255)
                       gAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCCtttttccg                                                                            >  1:1055919/1‑98 (MQ=255)
                          gCAAGATAAAGACTTGCGAGGTGTTCTTCATTTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGACGCCCCCC.GTTTTCCGTCa                                                                        >  2:159231/1‑101 (MQ=255)
                          gCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCa                                                                        >  1:3397081/1‑101 (MQ=255)
                          gCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCa                                                                        >  2:2215410/1‑101 (MQ=255)
                           cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAg                                                                       >  1:1932110/1‑101 (MQ=255)
                           cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAg                                                                       >  2:4401293/1‑101 (MQ=255)
                             aGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCg                                                                     >  1:2580543/1‑101 (MQ=255)
                               aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                                   >  2:3626346/1‑101 (MQ=255)
                                tAAAGACTTGCGAGGTGTTCTTCATGTTTCTGCAAAGA.CCGCGGGGGG.AACTTGCAGAGC.AAAAAGTGGGG.GGCGAAGCGGCCC.TTTTTCCGTCAGCGaaa                                                                  >  2:2916379/1‑101 (MQ=255)
                                 aaaGACTTGCGAGGTTTTTTTCATTTTTCTGAAAAGC.CCGGGGGGGG.AATTTGCACAG.AAAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                                 <  1:3504072/101‑1 (MQ=255)
                                 aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                                 >  1:260490/1‑101 (MQ=255)
                                  aaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACa                                                                >  2:1751240/1‑101 (MQ=255)
                                   aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAg                                                               >  2:2536706/1‑101 (MQ=255)
                                    gACTTGCGAGGTTTTCTTCATCTTTCTGAAAAGA.CCGGGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGc                                                              <  2:3089714/101‑1 (MQ=255)
                                    aagctgtggtgtgtTTTTCTTCTTTTTCCAAAAAGCCGGGGGGGGAAATTTGC.CAAAAAAAAGGGGGGG.CCGCCCCCCCCCCCTTTTTCCGTCAGCGAAAc                                                                 <  1:1979021/91‑1 (MQ=255)
                                      ttgggggggTTTTCTTCTTCTTCCGGAAAAGC.CCG.GGGGGG.AATTTGGCAAAAAAAAAGGGGGGG.CGC.AACCCCCCCCTTTTTCCGTCAGCGAAACAGCAc                                                            <  1:1190861/94‑1 (MQ=255)
                                      cTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAc                                                            >  2:13225/1‑101 (MQ=255)
                                      cTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCAGTCAGCGAAACAGCAc                                                            >  1:1506369/1‑101 (MQ=255)
                                          cGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGCGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                                        <  2:1647260/101‑1 (MQ=255)
                                           gAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                       >  2:2436520/1‑101 (MQ=255)
                                           gAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                       >  1:1690962/1‑101 (MQ=255)
                                           gAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                       >  1:4214829/1‑101 (MQ=255)
                                            aGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                                      >  2:1402098/1‑101 (MQ=255)
                                            aGGTGTCCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                                      <  2:593723/101‑1 (MQ=255)
                                               tgtTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGcc                                                   >  1:1398632/1‑101 (MQ=255)
                                                gtTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAATCAGCACCAGCGCGCCg                                                  >  1:2930938/1‑101 (MQ=255)
                                                  tcttcATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                                >  1:1759606/1‑101 (MQ=255)
                                                  tcttcATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                                >  1:3633683/1‑101 (MQ=255)
                                                     tcATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGa                                             >  2:755741/1‑101 (MQ=255)
                                                         cTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                         >  1:318626/1‑101 (MQ=255)
                                                         cTTTCTGCAAAGA.CCGCGGGGGG.AATTTCCACAGCAAAAAA.GGGGG.CGGGAAGCCCCCC.TTTTTCCGTCGGCGAAACAGCACGAGCGCGCCGCGCGACCAg                                         >  1:3914893/1‑101 (MQ=255)
                                                         cTTTCTGAAAAGC.CCGCGGGGGA.AATTTGCACAGC.AAAAAGGGGGG.CGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                         <  1:2293865/101‑1 (MQ=255)
                                                          tttCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                                        >  2:820088/1‑101 (MQ=255)
                                                          tttCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                                        >  1:1290095/1‑101 (MQ=255)
                                                           ttCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                                       >  1:460674/1‑101 (MQ=255)
                                                           ttCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                                       >  1:3065464/1‑101 (MQ=255)
                                                            tCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                                      >  1:4471471/1‑101 (MQ=255)
                                                            tCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                                      >  1:717605/1‑101 (MQ=255)
                                                             cTGAAAAGA.CCTCGGGGGG.AATTTGCACAGC.AAAAGGGGGGG.CGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                                     <  2:3140801/101‑1 (MQ=255)
                                                               gCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                                   >  2:1462942/1‑101 (MQ=255)
                                                               gCAAAGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                                   >  1:4356214/1‑101 (MQ=255)
                                                                gaaagcgccGGGGGGGA.AATGTCCACAGC.AAAAGGGGGGG.CGAG.ACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                                   <  2:4607861/94‑1 (MQ=255)
                                                                 ggagagcccggggggg.AAAATGCCAAGAAAAAAGGGGGGGCCGCCACCCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                                     <  2:4056182/91‑1 (MQ=255)
                                                                 aaaGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAGCCAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                                 >  1:4074974/1‑101 (MQ=255)
                                                                 aaaGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                                 >  2:4466188/1‑101 (MQ=255)
                                                                 aaaGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                                 >  2:1051207/1‑101 (MQ=255)
                                                                 aaaGA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                                 >  2:2001444/1‑101 (MQ=255)
                                                                    gA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAGCCGCGt                              >  1:772566/1‑101 (MQ=255)
                                                                    gA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                              >  2:34058/1‑101 (MQ=255)
                                                                    gA.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                              >  1:4453409/1‑101 (MQ=255)
                                                                     a.CCGCGGGGGG.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                             >  1:4293476/1‑101 (MQ=255)
                                                                            gggtg.AAGTTGCACCGC.AAAAAGGGGGG.CGCGAAACCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg                       <  2:3171625/101‑1 (MQ=255)
                                                                            ggggg.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg                       >  1:822229/1‑101 (MQ=255)
                                                                              ggg.AATTTGCACAGC.AAAAGGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc                     <  1:4717039/101‑1 (MQ=255)
                                                                               gg.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg                    >  2:2206611/1‑101 (MQ=255)
                                                                               gg.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg                    >  1:303805/1‑101 (MQ=255)
                                                                               gg.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGGCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGCGTGCCg                    >  2:2077332/1‑101 (MQ=255)
                                                                                g.AATTTGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGa                   >  2:3082405/1‑101 (MQ=255)
                                                                                      tGCACAGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc              >  2:1793144/1‑101 (MQ=255)
                                                                                       tcacaGC.AAAAAGGGGGG.CGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa             <  2:2281517/100‑1 (MQ=255)
                                                                                        cacaGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAg            >  1:2279217/1‑101 (MQ=255)
                                                                                        cacaGC.AAAAAGGGGGG.CGC.AAACCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAg            <  2:275621/101‑1 (MQ=255)
                                                                                         cgcagcaaaagGGGGGG.CGCGAGGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAg            <  1:643568/95‑1 (MQ=255)
                                                                                         acaGC.AAAAAGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGc           >  1:1896142/1‑101 (MQ=255)
                                                                                           gagcaaaagGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCa          <  2:4744054/97‑1 (MQ=255)
                                                                                               aaaaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCgg      >  2:4244023/1‑101 (MQ=255)
                                                                                               aaaaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCgg      <  1:498804/101‑1 (MQ=255)
                                                                                               aaaaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCgg      >  2:2600276/1‑101 (MQ=255)
                                                                                               aaaaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCgg      >  1:3678845/1‑101 (MQ=255)
                                                                                                aaaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCggg     <  1:2324468/101‑1 (MQ=255)
                                                                                                 aaaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGc    >  2:1110571/1‑101 (MQ=255)
                                                                                                  aaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGGACCAGCGCGCCGGGCGACCGGGCGACCCCCCGCTGGGTGCCGACATCCAACACCGGCCg   >  2:1903149/1‑101 (MQ=255)
                                                                                                  aaGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCg   >  2:3515919/1‑101 (MQ=255)
                                                                                                   aGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCgc  >  2:3887834/1‑100 (MQ=255)
                                                                                                   aGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCGa  >  2:534676/1‑101 (MQ=255)
                                                                                                   aGGGGGG.CGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCGa  >  2:4035154/1‑101 (MQ=255)
                                                                                                   |                                                                                                      
ATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGA.CCGCGGGGGG.AATTTGCACAGCAAAAAAGGGGGG.CGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCGGGCGA  >  NC_014034/2044178‑2044376

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG