Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,045,611 T→G 100% G208G (GGA→GGC)  potI2 ← polyamine ABC transporter permease PotI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,045,6110T→G100.0% 580.2 157G208G (GGA→GGC) potI2polyamine ABC transporter permease PotI
Reads supporting (aligned to +/- strand):  new base (123/33):  ref base (0/0):  total (123/34)

Only 100 of 220 total aligned reads displayed.

GAGCAGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCTCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAGGGCAGGATGA  >  NC_014034/2045513‑2045708
                                                                                                   |                                                                                                 
  gCAGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCg                                                                                               <  2:2583054/101‑1 (MQ=255)
   cAGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGc                                                                                              >  1:4158544/1‑101 (MQ=255)
   cAGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGc                                                                                              >  2:2106442/1‑101 (MQ=255)
    aGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGcc                                                                                             >  1:3799861/1‑101 (MQ=255)
    aGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGcc                                                                                             <  2:3308389/101‑1 (MQ=255)
         acAAGCACGGTGCCAAGCGCCATGATCAGCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCCCGCCCGCCagaaa                                                                                        <  1:3611171/101‑1 (MQ=255)
          cacggaCGGTGCCAGGCCCCAGGATCATCGGGATTTGTTTCGGAAA.GCGCGGCTGCAAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCagaaag                                                                                       <  2:3146966/96‑1 (MQ=255)
           aggCACGGTGCCAGGCGCCATGATCATGGGGATTTGTTTCGGAAA.GCGCAGCTGCGAATAGATATGGGTGGGCAGCGTCGTCTGCTCGCCCGCCagaaaga                                                                                      <  2:1726617/99‑1 (MQ=255)
           aaGCCCGGTGCCAAGCGCCATGATCATCGGTATCTGTTTCGGAAG.GCGCAGCGGCGAATAGATATAGGGGGGCAGCGTCGTCTGCCCGCCCGCCagaaaga                                                                                      <  2:4435343/101‑1 (MQ=255)
            aGCAGGGGGCCAAGCGCCATGATCAGCGGGATCTTTTTCGAAAA.GCGCAGCTGCGAATAGATATGGGTGGGCGGCGTCGTCTGCTCGCCCGCCagaaagaa                                                                                     <  2:549054/101‑1 (MQ=255)
               aCGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGc                                                                                  >  2:1006255/1‑101 (MQ=255)
               aCGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGc                                                                                  >  2:3498211/1‑101 (MQ=255)
                cGGTGCCAGGCGCCATGNTCATGGGGATTTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCCCGCCCGCCAGAAAGAAGGCg                                                                                 <  2:1287591/101‑1 (MQ=255)
                       aaGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGAtgatga                                                                          >  1:2138126/1‑101 (MQ=255)
                       aaGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGAtgatga                                                                          >  2:4008717/1‑101 (MQ=255)
                         gcgcCATGATCATGGGGATTTGTTTCGGAAA.GCGCAGCTGGAAATAGATATGGGTGGGCAGCGTCTTCTGCCCGCCCGCCAGAAAGAAGGCGATGATGAAt                                                                        <  2:3442331/101‑1 (MQ=255)
                         gccccgtGACCATGGGGTTTTGTTGCGAAAA.GCGCAGTTGAAAATAGATATGGGGGGGCCGCGTCGTTTGCCCGCCCGCCAGAAAGAAGGCGATGATGAAt                                                                        <  1:672616/95‑1 (MQ=255)
                          cgcCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAAtt                                                                       >  2:4217175/1‑101 (MQ=255)
                          cgcCAGGATCATGGGTATTTTTTTCGAAAA.GCGCGGCTGCAAATAGATATGGGTGGGCGGCGTCGTCTGCCCCCCCGCCAGAAAGAAGGCGATGATGAAtt                                                                       <  2:2735369/101‑1 (MQ=255)
                           cccATGATCATCGGGATCTGTTTCGGAAC.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTc                                                                      <  2:1902824/100‑1 (MQ=255)
                            ccATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCa                                                                     >  2:2132773/1‑101 (MQ=255)
                            ccATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCa                                                                     >  1:2548391/1‑101 (MQ=255)
                             cATGATCATGGGGATTTTTTTCGAAAA.GCGCAGCTGCAAATAAATATAGGGGGGCAGCGTCTTCTGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCAt                                                                    <  1:2650316/101‑1 (MQ=255)
                             cATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCAt                                                                    >  2:2992927/1‑101 (MQ=255)
                             cATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCAt                                                                    >  2:4555276/1‑101 (MQ=255)
                             cATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCAt                                                                    >  1:3713004/1‑101 (MQ=255)
                              atggtcagggggTTCTGTTGCGAAAA.GCGCAGCTGAGAATAGATATGGGGGGGCGGCGTCTTCTCCCCGCCCGCCAGAAAGAAGGCGATGATGAATTCATc                                                                   <  1:4652978/92‑1 (MQ=255)
                              aTGATCATCGGGATCTGTTTGGAAAA.GCGCAGCTGCGAATAGATATAGGGGGGCAGCGTCGTCTGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATc                                                                   <  1:503241/101‑1 (MQ=255)
                              aTGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATc                                                                   >  2:2167779/1‑101 (MQ=255)
                              aTGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATc                                                                   >  2:3694257/1‑101 (MQ=255)
                              aTGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATc                                                                   >  2:2683561/1‑101 (MQ=255)
                               tGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATcc                                                                  >  1:1057908/1‑101 (MQ=255)
                               ggATGATGGGGATTTCTTTCGGAAA.GCCCGCCAGCGAAAGGATTTGGGGGGGCAGCGGCGTCGACTCCCCCGCCAGAAAGAAGGCGATGATGAATTCATcc                                                                  <  2:429511/100‑1 (MQ=255)
                                gATCGTCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCa                                                                 >  2:10319/1‑101 (MQ=255)
                                gATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCAAATAAATATAGGGGGGCAGCGTCGTCTGCCCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCa                                                                 <  1:386643/101‑1 (MQ=255)
                                 atcatcGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAg                                                                >  2:4452127/1‑101 (MQ=255)
                                  tcatcGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGc                                                               >  2:1369977/1‑101 (MQ=255)
                                   catcGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCg                                                              >  2:3396426/1‑101 (MQ=255)
                                     tcGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCgag                                                            >  1:1526772/1‑101 (MQ=255)
                                     tcGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCgag                                                            <  1:3694487/101‑1 (MQ=255)
                                      cGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCgaga                                                           >  1:206439/1‑101 (MQ=255)
                                          ttttttttGGGAAA.GCGCGCCTGCAAAAAAATAGGGGGGGGCAGCGTCGTCTCCCCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGt                                                       <  2:733575/96‑1 (MQ=255)
                                          aTCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGt                                                       >  1:3366870/1‑101 (MQ=255)
                                            cTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGa                                                     >  1:4720719/1‑101 (MQ=255)
                                            cTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGa                                                     >  2:2306646/1‑101 (MQ=255)
                                             tGTTTCGGAAA.GCGCAGCTGCGAATAGATATGGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGaa                                                    >  1:4230029/1‑101 (MQ=255)
                                             tGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGaa                                                    >  1:1199835/1‑101 (MQ=255)
                                              tgttgggAAAGGCGCAGCTGCGAATAGATATAGGGGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGaa                                                    <  2:1615052/96‑1 (MQ=255)
                                               tttCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAgg                                                  >  2:1326599/1‑101 (MQ=255)
                                                ttCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGc                                                 >  1:2417928/1‑101 (MQ=255)
                                                  gggAAA.GCGCAGCGGCGAATAGATATGGGGGGGGAGCGTCGTCGGCGCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCa                                               <  1:2445340/100‑1 (MQ=255)
                                                  cGGAAA.GCGCAGCGGCAAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCa                                               <  2:2871111/101‑1 (MQ=255)
                                                   agAAA.GCCCGGGTGCAAATAGATATGGGGGGGCCGCGTCGTCTGCCCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCAt                                              <  2:874856/100‑1 (MQ=255)
                                                    gaaaggcgcAGCTGAGAATAGATATGGGGGGGCGCCGCCTTCTGCCCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCAt                                              <  2:4048087/96‑1 (MQ=255)
                                                    gAAG.GCGCAGCTGCAAATAGATATGGGGGGGCAGCGTCGTCTGCTCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATc                                             <  1:1112038/101‑1 (MQ=255)
                                                    gAAA.GCGCATCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATc                                             <  2:3586969/101‑1 (MQ=255)
                                                    gAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATc                                             >  1:3227869/1‑101 (MQ=255)
                                                    gAAA.GCGCAGCTGCGAATAGATATAGGGGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATc                                             >  2:1159796/1‑101 (MQ=255)
                                                    gAAA.GCGCAGCTGCGAATAGATAGGGGTGGGCAGCGTCGTCTGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATc                                             <  1:2469476/101‑1 (MQ=255)
                                                     aaa.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCa                                            >  2:3019229/1‑101 (MQ=255)
                                                      aa.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAg                                           <  2:177560/101‑1 (MQ=255)
                                                      aa.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAg                                           >  1:4371878/1‑101 (MQ=255)
                                                       aggcgcAGCGGCAAAAAGATATGGGGGGGGAGGGTCGTCTCCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAg                                           <  1:3005688/99‑1 (MQ=255)
                                                       a.GCGCAGCTGCAAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAgc                                          <  2:3787103/101‑1 (MQ=255)
                                                          cgcAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAgcgc                                        >  2:3651632/1‑101 (MQ=255)
                                                          cgcAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAgcgc                                        >  2:940886/1‑101 (MQ=255)
                                                           gcAGATGCGAATAGATATAGGTGGGAAGGGCCGTTTGCGCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGcgcc                                       <  1:1537247/101‑1 (MQ=255)
                                                             aGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGcgccgc                                     >  2:48184/1‑101 (MQ=255)
                                                                 gCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCAAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGaa                                 <  1:1470709/101‑1 (MQ=255)
                                                                   gAATAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAAt                               >  2:2930828/1‑101 (MQ=255)
                                                                   aaagaGATATGGGGGGGCGGCGTCGTCCGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAAt                               <  2:2493359/97‑1 (MQ=255)
                                                                    aaTAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATg                              >  1:653091/1‑101 (MQ=255)
                                                                    aaTAGATAGGGGGGGGCGCCGTCTTCTGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATg                              <  1:569398/101‑1 (MQ=255)
                                                                     aTAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGa                             <  1:4452377/101‑1 (MQ=255)
                                                                     aTAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGa                             >  1:1825860/1‑101 (MQ=255)
                                                                      tAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGAt                            >  2:1181468/1‑101 (MQ=255)
                                                                      tAGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGAt                            <  1:4555529/101‑1 (MQ=255)
                                                                       aGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATc                           >  2:3330750/1‑101 (MQ=255)
                                                                       aGATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATc                           <  1:1996724/101‑1 (MQ=255)
                                                                        gATATGGGTGGGCTGCGTCGTCTCCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATcc                          <  2:459219/101‑1 (MQ=255)
                                                                        gATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATcc                          >  1:1735145/1‑101 (MQ=255)
                                                                        gATATAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCAATCAGCGCCGCGGAAATGATcc                          >  1:3891596/1‑101 (MQ=255)
                                                                        aattggggggggCCGCGTCTTCTCCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATcc                          <  1:3055452/92‑1 (MQ=255)
                                                                         atatAGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATccc                         >  2:3232963/1‑101 (MQ=255)
                                                                          tataGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATcccc                        >  1:2811038/1‑101 (MQ=255)
                                                                           ataGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCg                       >  2:168338/1‑101 (MQ=255)
                                                                             aGGTGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGc                     >  1:2160576/1‑101 (MQ=255)
                                                                                tGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTc                  >  1:2344375/1‑101 (MQ=255)
                                                                                tGGGCAGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTc                  >  2:1512824/1‑101 (MQ=255)
                                                                                gggcccgCGTCGTCCCCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTc                  <  1:3831003/95‑1 (MQ=255)
                                                                                     gccgccgtcTCCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCaa             <  1:1247319/96‑1 (MQ=255)
                                                                                     aGCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCaa             >  2:4482292/1‑101 (MQ=255)
                                                                                      gCGTCGTCTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAg            <  2:1549976/101‑1 (MQ=255)
                                                                                        gtcgtcTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCTGCGGAAATGATCCCCGGCGTCACCAAggg          >  2:1453658/1‑101 (MQ=255)
                                                                                        gtcgtcTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAggg          >  1:1156167/1‑101 (MQ=255)
                                                                                        gtcgtcTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAggg          >  1:3550435/1‑101 (MQ=255)
                                                                                        gtcctcTGCCCCCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAggg          <  1:222972/101‑1 (MQ=255)
                                                                                         tcgtcTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAGGGc         >  1:844308/1‑101 (MQ=255)
                                                                                             cTGCTCGCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAGGGCAGga     >  2:2612713/1‑101 (MQ=255)
                                                                                                ctcgCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAGGGCAGgatga  >  2:1957362/1‑101 (MQ=255)
                                                                                                   |                                                                                                 
GAGCAGACGACAAGCACGGTGCCAAGCGCCATGATCATCGGGATCTGTTTCGGAAA.GCGCAGCTGCGAATAGATATAGGTGGGCAGCGTCGTCTGCTCTCCCGCCAGAAAGAAGGCGATGATGAATTCATCCAGCGAGATGGTGAAGGCCATCAGCGCCGCGGAAATGATCCCCGGCGTCACCAAGGGCAGGATGA  >  NC_014034/2045513‑2045708

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG