BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,057,834	A→C	10.7%	intergenic (‑600/‑302)	RCAP_rcc01900 ← / → RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,057,834	0	A→C	10.7%	46.4 ‑1.9 ‑0.3	178	intergenic (‑600/‑302)	RCAP_rcc01900/RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand): new base (13/6): ref base (76/83): total (89/89)
Fisher's exact test strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.03e-01

Only 100 of 182 total aligned reads displayed.


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGca                                                                                >  1:2186292/1‑100 (MQ=255)


     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAC.T.CC.CG.CGC.CGcaagcg                                                                            >  2:239297/1‑96 (MQ=255)


      tccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgt                                                                           <  2:1317534/100‑7 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGGGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                          <  1:1088807/101‑8 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGCCAA.T.CC.CG.CGC.CGcaagcgttt                                                                         >  1:2117822/1‑93 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgttt                                                                         <  1:2832507/101‑9 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgttt                                                                         >  2:3587988/1‑93 (MQ=255)


         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtttc                                                                        >  2:2756367/1‑92 (MQ=255)


          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGccagcgtttcc                                                                       >  2:2613456/1‑91 (MQ=255)


                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                   <  1:2835799/101‑1 (MQ=255)


                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                   >  2:1445076/1‑101 (MQ=255)


                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                   <  1:1642245/101‑1 (MQ=255)


                   ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCa                                                                  <  1:66211/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  1:2109880/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:1134113/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:476038/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:481308/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 >  1:3070863/1‑101 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:883138/101‑1 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:4449913/101‑1 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               >  2:3128791/1‑101 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               >  2:546185/1‑101 (MQ=255)


                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCat                                                              <  2:2145238/101‑2 (MQ=255)


                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCat                                                              <  2:3929740/101‑2 (MQ=255)


                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                             >  2:3956780/1‑99 (MQ=255)


                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            <  2:2573330/101‑1 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  2:4421279/1‑101 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:824865/1‑101 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:1043175/1‑101 (MQ=255)


                           ggCGCGCGGACTTCGGGGGGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  2:1293430/101‑1 (MQ=255)


                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:392945/101‑1 (MQ=255)


                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                         <  2:892670/101‑1 (MQ=255)


                             cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                        <  2:3657945/101‑1 (MQ=255)


                                gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    <  2:3561699/101‑1 (MQ=255)


                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  2:2283930/101‑1 (MQ=255)


                                  ggccTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  2:4571443/98‑1 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  1:4687331/101‑1 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  2:1224380/101‑1 (MQ=255)


                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:740208/1‑99 (MQ=255)


                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  2:530390/1‑97 (MQ=255)


                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  1:1345797/101‑5 (MQ=255)


                                       tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  1:297722/101‑6 (MQ=255)


                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                             <  1:4750815/101‑1 (MQ=255)


                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            >  1:4707809/1‑101 (MQ=255)


                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            <  2:4692896/101‑1 (MQ=255)


                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                           >  2:2055603/1‑101 (MQ=255)


                                            gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACa                                         <  2:3426366/101‑1 (MQ=255)


                                             cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        <  1:4279598/101‑1 (MQ=255)


                                             atccggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        >  2:1893523/4‑101 (MQ=255)


                                              ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  2:3540669/1‑101 (MQ=255)


                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  2:3256261/101‑1 (MQ=255)


                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  2:15036/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  1:3517525/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  1:4518980/1‑101 (MQ=255)


                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:1045976/1‑101 (MQ=255)


                                                     gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  2:2692896/101‑1 (MQ=255)


                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  1:1947064/1‑101 (MQ=255)


                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgcc                             <  1:1249167/101‑1 (MQ=255)


                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:1185803/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:2918187/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:398826/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:3138544/101‑1 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATGGGCGACAACC.CCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  2:2117701/101‑1 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:3378195/1‑101 (MQ=255)


                                                             gCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        >  1:162260/1‑101 (MQ=255)


                                                              cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  1:267189/1‑101 (MQ=255)


                                                              cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:4287722/101‑1 (MQ=255)


                                                               gATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:1812988/1‑101 (MQ=255)


                                                                aTCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     >  2:1354625/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3837664/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3736562/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:4084153/1‑101 (MQ=255)


                                                                  ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:1336364/101‑1 (MQ=255)


                                                                  ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:1713669/101‑1 (MQ=255)


                                                                   cTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:1524027/1‑101 (MQ=255)


                                                                    tGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:21431/1‑101 (MQ=255)


                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:462118/1‑101 (MQ=255)


                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:4672040/101‑1 (MQ=255)


                                                                     gCGTTTCCGGATGGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:3333193/101‑1 (MQ=255)


                                                                      cGTTTCCGGATTGGCGGCAA.TCCCGCGCCGCAAGCGTTTCCGAACTGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:3211606/101‑1 (MQ=255)


                                                                      cGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               >  1:3665906/1‑101 (MQ=255)


                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4381347/1‑101 (MQ=255)


                                                                        tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  1:4206335/1‑101 (MQ=255)


                                                                        tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4472119/1‑101 (MQ=255)


                                                                         ttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:3723001/1‑101 (MQ=255)


                                                                         ttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:3085795/1‑101 (MQ=255)


                                                                          tCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:3845429/1‑101 (MQ=255)


                                                                           ccGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  1:792258/1‑101 (MQ=255)


                                                                           ccGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  2:1714359/1‑101 (MQ=255)


                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:3458998/1‑101 (MQ=255)


                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:1144476/1‑101 (MQ=255)


                                                                             ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:2953626/1‑101 (MQ=255)


                                                                              gATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:546236/101‑1 (MQ=255)


                                                                               aTTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:3723231/101‑1 (MQ=255)


                                                                                ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:583761/101‑1 (MQ=255)


                                                                                ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  1:3311679/1‑101 (MQ=255)


                                                                                ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTCCGATCaa     >  2:3063622/1‑101 (MQ=255)


                                                                                 tGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  2:3774145/1‑101 (MQ=255)


                                                                                  ggCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   >  2:3841478/1‑101 (MQ=255)


                                                                                   gCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  >  2:2811096/1‑101 (MQ=255)


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG