BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,123,404	A→G	3.1%	P256P (CCT→CCC)	wza ←	polysaccharide export protein Wza

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,123,404	0	A→G	3.1%	5.6 0.0 ‑0.0	191	P256P (CCT→CCC)	wza	polysaccharide export protein Wza
Reads supporting (aligned to +/- strand): new base (3/3): ref base (91/94): total (94/97)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 209 total aligned reads displayed.


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCC  >  NC_014034/2123306‑2123505


aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                      <  1:4722306/101‑1 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                     <  1:733811/101‑2 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                     >  1:4543553/1‑100 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                     <  1:4339963/101‑2 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                     >  1:2979330/1‑100 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGGGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                     >  1:424296/1‑100 (MQ=255)


  gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                                   >  1:1369051/1‑101 (MQ=255)


   aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                                  <  2:244636/101‑1 (MQ=255)


    tGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTg                                                                                                 <  2:1096656/101‑1 (MQ=255)


     gCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGa                                                                                                <  1:1270147/101‑1 (MQ=255)


        gcgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcga                                                                                             <  1:1156474/101‑3 (MQ=255)


         cgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgat                                                                                            <  1:785407/101‑4 (MQ=255)


         cgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgat                                                                                            >  1:69879/1‑98 (MQ=255)


           ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                          <  2:713825/101‑6 (MQ=255)


            gCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctg                                                                                         <  2:918895/101‑7 (MQ=255)


              aTCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggc                                                                                       >  2:2955482/1‑93 (MQ=255)


                cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                                     >  2:1733649/1‑91 (MQ=255)


                        tCCTCGGCCAGGCCGCCGGCGCGGGTTAGCGCCTGCGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATa                                                                             <  1:3859286/101‑1 (MQ=255)


                           tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                          >  1:2094450/1‑101 (MQ=255)


                            cGGCCAGACAGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcc                                                                         >  1:2113319/1‑101 (MQ=255)


                             ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGGGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcct                                                                        >  2:424128/1‑101 (MQ=255)


                              gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctc                                                                       <  1:4553685/101‑1 (MQ=255)


                              gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctc                                                                       >  2:641599/1‑101 (MQ=255)


                               ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcc                                                                      >  1:2234418/1‑101 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCCGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCTTg                                                                    <  1:3992791/101‑1 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                    >  2:449548/1‑101 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                    >  2:2081133/1‑101 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                    <  1:3609563/101‑1 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                    <  2:3814173/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                                 <  2:1378590/101‑1 (MQ=255)


                                     cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                >  2:3251664/1‑101 (MQ=255)


                                       ccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGt                                                              <  1:1879912/101‑1 (MQ=255)


                                          gcgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                           <  1:703575/100‑1 (MQ=255)


                                          aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACNGNCTg                                                           >  1:1439798/1‑101 (MQ=255)


                                          aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                           <  1:1445363/101‑1 (MQ=255)


                                          aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                           <  1:2423847/101‑1 (MQ=255)


                                                gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                     >  1:2447606/1‑101 (MQ=255)


                                                gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGAGCGTCG.AGGGCTTGACGATCAGGCTCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                     >  1:4062554/1‑101 (MQ=255)


                                                 tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                    >  2:1741667/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                   >  2:1310096/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                   >  2:3343546/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                   <  2:4543300/101‑1 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                   >  1:1819736/1‑101 (MQ=255)


                                                   aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                                  >  1:1048949/1‑101 (MQ=255)


                                                     cGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGc                                                >  2:1558355/1‑101 (MQ=255)


                                                          gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTnacg                                           >  1:3897136/1‑101 (MQ=255)


                                                            gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                         >  1:2484016/1‑101 (MQ=255)


                                                            gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                         <  1:449592/101‑1 (MQ=255)


                                                            gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                         >  1:3294179/1‑101 (MQ=255)


                                                            gTCAGGGTGATGTTTTCCAGCGTCAGATCGACCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                         <  1:4267503/101‑1 (MQ=255)


                                                             tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                        <  2:4665205/101‑1 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATTGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                       <  2:3078644/101‑1 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                       >  1:1505667/1‑101 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                       <  1:3213723/101‑1 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                       >  1:4458588/1‑101 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                      <  1:4385190/101‑1 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGAAATc                                      >  2:875170/1‑101 (MQ=255)


                                                                 ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                    >  1:642014/1‑101 (MQ=255)


                                                                 ggTGATGTTTTACAGCCTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAGCGTCTGCCGCACGACGCTGACGACATCGc                                    <  2:2832080/101‑1 (MQ=255)


                                                                 cacgttGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCAGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                    >  2:4099458/6‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                   >  2:4686376/1‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                   >  2:789796/1‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                   >  1:814095/1‑101 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                  >  2:1629003/1‑101 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                  >  1:4213002/1‑101 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                  <  1:1723191/101‑1 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                  >  2:261958/1‑101 (MQ=255)


                                                                      tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                               <  1:3798599/101‑1 (MQ=255)


                                                                      tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                               >  1:2630229/1‑101 (MQ=255)


                                                                       gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                              >  2:1414947/1‑101 (MQ=255)


                                                                         tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                            >  2:1277824/1‑101 (MQ=255)


                                                                         tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                            >  1:2020491/1‑101 (MQ=255)


                                                                          ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGACCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                           <  2:2433632/101‑1 (MQ=255)


                                                                           tCCAGCGTCGGAGCGATCGTCG.AGGGCTTGACGATCTGGGCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                          <  2:1890826/101‑1 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                         >  1:3080700/1‑101 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                         <  2:1048870/101‑1 (MQ=255)


                                                                              aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                       >  2:2142841/1‑101 (MQ=255)


                                                                               gCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                      <  2:2113198/101‑1 (MQ=255)


                                                                                 gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                    >  2:2878973/1‑101 (MQ=255)


                                                                                  tCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggg                   >  1:1008587/1‑101 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                  >  2:3773539/1‑101 (MQ=255)


                                                                                    tgatcgatcGTCG.AGGGCTTGACGCTCTGGCCCACTAGATAGGCCTCATGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt                 <  2:2687694/100‑1 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                >  1:563677/1‑101 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                <  2:4210699/101‑1 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                >  2:3496536/1‑101 (MQ=255)


                                                                                      atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt               <  1:2726286/101‑1 (MQ=255)


                                                                                        cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg             <  1:1589626/101‑1 (MQ=255)


                                                                                         taatggtcgaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCCGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg             <  2:3603299/92‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg            >  2:1939762/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg            <  2:2629712/101‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg            >  1:2557278/1‑101 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc           >  2:3555789/1‑101 (MQ=255)


                                                                                           tcgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcg          <  1:3912086/101‑1 (MQ=255)


                                                                                            cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc         >  2:4390443/1‑101 (MQ=255)


                                                                                            cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc         >  2:1592747/1‑101 (MQ=255)


                                                                                            cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc         >  2:3124550/1‑101 (MQ=255)


                                                                                                 gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcccag     >  2:2003561/2‑98 (MQ=255)


                                                                                                 gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt     >  2:3306286/2‑101 (MQ=255)


                                                                                                 gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt     >  2:3963912/2‑101 (MQ=255)


                                                                                                 gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt     >  2:4429266/2‑101 (MQ=255)


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCC  >  NC_014034/2123306‑2123505

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG