Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,424 A→G 100% M250M (TTG→CTG)  wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4240A→G100.0% 773.1 203M250M (TTG→CTG) wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (102/100):  ref base (0/0):  total (102/101)

Only 100 of 207 total aligned reads displayed.

TCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGCC  >  NC_014034/2123330‑2123521
                                                                                               |                                                                                                 
  cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                                           <  1:1489596/101‑1 (MQ=255)
    cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcc                                                                                         <  1:3876966/101‑1 (MQ=255)
    cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcc                                                                                         >  1:3898243/1‑101 (MQ=255)
     ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcct                                                                                        >  2:2398671/1‑101 (MQ=255)
       ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcc                                                                                      >  1:2234418/1‑101 (MQ=255)
        cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcct                                                                                     <  2:3282689/101‑1 (MQ=255)
         aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCCGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCTTg                                                                                    <  1:3992791/101‑1 (MQ=255)
         aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                                    >  2:2081133/1‑101 (MQ=255)
          gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                                   <  1:210283/101‑1 (MQ=255)
          gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                                   <  1:2081254/101‑1 (MQ=255)
            ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGCTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:4049727/101‑1 (MQ=255)
            ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  2:1378590/101‑1 (MQ=255)
             cgccgACGCGGGTGATCGCCTGGGTCATGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                                <  2:1732700/101‑1 (MQ=255)
             cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                                >  2:3251664/1‑101 (MQ=255)
                cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                                             <  1:4716244/101‑1 (MQ=255)
                  gcgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                                           <  1:703575/100‑1 (MQ=255)
                  aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                                           <  2:3316368/101‑1 (MQ=255)
                    gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                                         <  1:2446936/101‑1 (MQ=255)
                       ggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcc                                                                      <  1:3118838/101‑1 (MQ=255)
                       ggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcc                                                                      >  2:274324/1‑101 (MQ=255)
                        gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGAGCGTCG.AGGGCTTGACGATCAGGCTCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                                     >  1:4062554/1‑101 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                   <  2:4543300/101‑1 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                   >  1:1819736/1‑101 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                   >  2:1310096/1‑101 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                   >  1:38542/1‑101 (MQ=255)
                             cGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGc                                                                >  1:1407053/1‑101 (MQ=255)
                                cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                                             >  2:1443974/1‑101 (MQ=255)
                                cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                                             <  2:887573/101‑1 (MQ=255)
                                cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                                             >  2:848574/1‑101 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         <  1:3315403/101‑1 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         >  1:3294179/1‑101 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         <  1:449592/101‑1 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         >  1:2484016/1‑101 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGACCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         <  1:4267503/101‑1 (MQ=255)
                                     tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                                        <  2:4665205/101‑1 (MQ=255)
                                      cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                                       >  1:120350/1‑101 (MQ=255)
                                      cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                                       >  1:4458588/1‑101 (MQ=255)
                                       aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                      <  1:3018842/101‑1 (MQ=255)
                                       aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                      >  1:42476/1‑101 (MQ=255)
                                       aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                      <  1:4385190/101‑1 (MQ=255)
                                        gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                                     <  2:424252/101‑1 (MQ=255)
                                         ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                                    >  1:642014/1‑101 (MQ=255)
                                         ggTGATGTTTTACAGCCTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAGCGTCTGCCGCACGACGCTGACGACATCGc                                                    <  2:2832080/101‑1 (MQ=255)
                                         cacgttGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCAGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                                    >  2:4099458/6‑101 (MQ=255)
                                          gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                                   >  2:789796/1‑101 (MQ=255)
                                          gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                                   >  1:814095/1‑101 (MQ=255)
                                           tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  >  2:261958/1‑101 (MQ=255)
                                           tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  <  1:1723191/101‑1 (MQ=255)
                                           tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  >  1:455056/1‑101 (MQ=255)
                                           tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  >  1:4213002/1‑101 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGCCGCTGACGACATCGCCGttt                                               >  2:706726/1‑101 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                               <  1:3798599/101‑1 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                               >  1:2630229/1‑101 (MQ=255)
                                                ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                                             >  2:3053280/1‑101 (MQ=255)
                                                ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                                             <  1:3078971/101‑1 (MQ=255)
                                                ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                                             <  1:3251865/101‑1 (MQ=255)
                                                 tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                                            >  1:2020491/1‑101 (MQ=255)
                                                 tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                                            >  2:1277824/1‑101 (MQ=255)
                                                  ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                                           <  2:361811/101‑1 (MQ=255)
                                                   tCCAGCGTCGGAGCGATCGTCG.AGGGCTTGACGATCTGGGCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                                          <  2:1890826/101‑1 (MQ=255)
                                                      aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                                       >  2:2142841/1‑101 (MQ=255)
                                                      aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                                       <  2:3782388/101‑1 (MQ=255)
                                                       gCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                                      <  2:559958/101‑1 (MQ=255)
                                                       gCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                                      <  2:2113198/101‑1 (MQ=255)
                                                        cGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg                                     >  1:1887547/1‑101 (MQ=255)
                                                         gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                                    >  2:2878973/1‑101 (MQ=255)
                                                         gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                                    >  1:1849748/1‑101 (MQ=255)
                                                          tCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggg                                   >  1:1008587/1‑101 (MQ=255)
                                                             gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                                >  1:563677/1‑101 (MQ=255)
                                                             gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                                <  2:4210699/101‑1 (MQ=255)
                                                             gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                                >  2:3496536/1‑101 (MQ=255)
                                                              atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt                               <  1:2726286/101‑1 (MQ=255)
                                                              atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt                               <  1:424172/101‑1 (MQ=255)
                                                                cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg                             <  1:1589626/101‑1 (MQ=255)
                                                                 gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg                            >  2:1939762/1‑101 (MQ=255)
                                                                 gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg                            >  1:2557278/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                         >  2:1592747/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                         >  2:4390443/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                         >  2:3124550/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                         >  1:4139543/1‑101 (MQ=255)
                                                                      ggcgaGGGCTTGGCGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcc                        <  1:1444068/97‑1 (MQ=255)
                                                                       tcgaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg                       <  2:1075578/98‑1 (MQ=255)
                                                                         gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcccag                     >  2:2003561/2‑98 (MQ=255)
                                                                         gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                     >  2:3963912/2‑101 (MQ=255)
                                                                         gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                     >  1:675407/2‑101 (MQ=255)
                                                                         gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                     >  2:4429266/2‑101 (MQ=255)
                                                                          aGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg                    <  2:360236/101‑1 (MQ=255)
                                                                          aGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg                    >  1:392944/1‑101 (MQ=255)
                                                                            ggCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGcc                  <  2:2501715/101‑1 (MQ=255)
                                                                            ggCTTGACGATCTGCCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGcc                  <  2:2668090/101‑1 (MQ=255)
                                                                              cTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt                <  1:1064736/101‑1 (MQ=255)
                                                                              cTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt                <  2:1584658/101‑1 (MQ=255)
                                                                                tgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGt              <  1:1028149/93‑1 (MQ=255)
                                                                                tgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGt              <  2:353776/93‑1 (MQ=255)
                                                                                 gacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGtt             >  1:2350556/8‑101 (MQ=255)
                                                                                     atctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAg         >  1:397863/4‑101 (MQ=255)
                                                                                     atctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCAAg         <  1:3486556/98‑1 (MQ=255)
                                                                                          gcccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAgg    >  2:4257/3‑101 (MQ=255)
                                                                                           cccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGc   <  1:880963/100‑1 (MQ=255)
                                                                                            ccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGcc  >  2:3402717/1‑101 (MQ=255)
                                                                                               |                                                                                                 
TCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGCC  >  NC_014034/2123330‑2123521

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG