Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 12.0% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C12.0% 57.8 0.0 ‑0.1208S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/13):  ref base (88/95):  total (100/108)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.73e-01

Only 100 of 215 total aligned reads displayed.

CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540
                                                                                                     |                                                                                                    
ccgccgCCCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAg                                                                                                      <  2:4545240/101‑1 (MQ=255)
 cgccgCCCCGGCCGCGTTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGc                                                                                                     <  1:2215915/101‑1 (MQ=255)
  gcggACCCGGCCGGGTTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                    <  2:3139756/98‑1 (MQ=255)
   ccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   >  1:1881287/1‑101 (MQ=255)
   cccccccCGCGGCGTTCCCCCTCACCTTCGGAGCCCCTCGGTCGCGGTCCCCGTCCCCCCCCCCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   <  1:1546165/97‑1 (MQ=255)
    cgccccGGCCCCGGCCCCCCCCCCCTTCGGATCCCCCCGGGCCCCGCCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  <  2:2682413/98‑1 (MQ=255)
      ccccGGCGGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCACCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  1:713962/100‑1 (MQ=255)
      ccccGGCCGCGGTCGCCCTCTCCTTCGGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  1:4637621/100‑1 (MQ=255)
      ccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCCCCGCCCCCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  1:280804/100‑1 (MQ=255)
       ccgggCCGCGGTCCCCCTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  1:3417329/98‑1 (MQ=255)
       cccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               >  2:1608601/1‑101 (MQ=255)
        ccGGCCGCGCTCGCCCTCTCCTTCTGACGCCCTGGGGCGCGGTCCCCTTCACCGCCCG.CGGGCGGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                              <  2:4350952/101‑1 (MQ=255)
          ggCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                            >  1:40632/1‑101 (MQ=255)
            ccGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                          >  1:2019448/1‑101 (MQ=255)
              ggggTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                        <  2:3103611/99‑1 (MQ=255)
              gcgGTCCCCCTCCCCTTCTGATGCCCTGGGGCCCCGCCCCCGTCCCCGCCCGCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                        <  1:1115620/101‑1 (MQ=255)
               gggTCGCCCTCCCCTTCGGATGCCCTGGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       <  2:3046055/100‑1 (MQ=255)
               cgGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       >  1:1272803/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  1:212927/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  2:3868530/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  1:1253517/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  2:855998/1‑101 (MQ=255)
                    gCCCTCACCTTCTGATGCCCTCGGCCCCCTTCACCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  <  2:3624312/101‑1 (MQ=255)
                    ccccTCGCCTTCTGACGCCCTGGGGCGCCGCCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  <  2:1020164/100‑1 (MQ=255)
                       cTCCCCTTCTGATGCCCGGGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               <  1:2741721/101‑1 (MQ=255)
                        tCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                              >  1:1070119/1‑101 (MQ=255)
                         cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                             >  1:4272219/1‑101 (MQ=255)
                         cACCTCCTGATGCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                             <  1:3093597/101‑1 (MQ=255)
                          ccctccggATCCCCTGGGGCGCCGTCCCCGTCACCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                            <  2:288287/94‑1 (MQ=255)
                            cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          >  1:2311888/1‑101 (MQ=255)
                            cTTCTGATGCCCCGGGGCGCCCCCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          <  2:79191/101‑1 (MQ=255)
                            cTTCTGATCCCCGCGGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          <  1:690800/101‑1 (MQ=255)
                               cTGATCCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                       <  2:2787443/101‑1 (MQ=255)
                                tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGTGCTCGTCGAtc                                                                      >  2:1241081/1‑100 (MQ=255)
                                tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAtc                                                                      >  2:1230738/1‑100 (MQ=255)
                                tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                      >  1:1145837/1‑101 (MQ=255)
                                 gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                     >  2:2497233/1‑101 (MQ=255)
                                          gcgcccccccaccgtccccgCCCGCGGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                            <  2:4524851/92‑1 (MQ=255)
                                            gcgcCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                          >  1:4343610/1‑101 (MQ=255)
                                              gcCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                        >  2:2827385/1‑101 (MQ=255)
                                              gcCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                        >  2:3098933/1‑101 (MQ=255)
                                               ccgtcaccgtcaCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgggg                                                       >  1:614574/1‑101 (MQ=255)
                                                 gtccccgcccccCCCCGCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     <  2:2707509/101‑1 (MQ=255)
                                                 gtcaccgtcaccGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  2:4662425/1‑101 (MQ=255)
                                                 gtcaccgtcaccGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCACTCTGGGGCGGGGcc                                                     >  2:4361462/1‑101 (MQ=255)
                                                 gcccccgcccccgcccgcGGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     <  1:3729284/91‑1 (MQ=255)
                                                     ccgtcaccgCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                 >  2:505689/1‑101 (MQ=255)
                                                     ccgcccccccccgcgGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                 <  1:2673994/92‑1 (MQ=255)
                                                       ccccccgcccgcCGGGGGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               <  1:147534/97‑1 (MQ=255)
                                                        tcaccgCCCGCGGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGCTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                              <  1:3188278/101‑1 (MQ=255)
                                                        tcaccgCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                              >  2:1697986/1‑101 (MQ=255)
                                                         cacccCCCGCCGGGCGC.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                             <  2:3011339/101‑1 (MQ=255)
                                                           ccccccgccGGGCAA.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                           <  1:3099424/98‑1 (MQ=255)
                                                                   ggggGGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   <  1:856072/100‑1 (MQ=255)
                                                                   cgGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   >  1:447258/1‑101 (MQ=255)
                                                                   cgGGCGA.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   >  2:1139421/1‑101 (MQ=255)
                                                                    gggCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGGCAACACGATggcggcgg                                  >  2:4470744/1‑101 (MQ=255)
                                                                     ggcgg.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                 >  1:4528714/1‑101 (MQ=255)
                                                                      ggcagtaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                 <  2:1321272/95‑1 (MQ=255)
                                                                       gaaaaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGgcggcggcg                                <  2:4281175/96‑1 (MQ=255)
                                                                       cgg.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                               >  1:2489939/1‑101 (MQ=255)
                                                                         ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                              <  2:1969897/98‑1 (MQ=255)
                                                                            aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGc                           >  1:1282401/1‑101 (MQ=255)
                                                                            aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                           >  1:3081161/1‑101 (MQ=255)
                                                                               ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCgg                        >  1:3636941/1‑101 (MQ=255)
                                                                                cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                       >  2:845873/1‑101 (MQ=255)
                                                                                   gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    <  2:4013712/101‑1 (MQ=255)
                                                                                   gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  1:1379711/1‑101 (MQ=255)
                                                                                     aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  >  1:701401/1‑101 (MQ=255)
                                                                                     aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  <  1:2428929/101‑1 (MQ=255)
                                                                                       ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGATGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                <  2:1874525/91‑1 (MQ=255)
                                                                                         gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  2:3332150/1‑101 (MQ=255)
                                                                                         gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  2:3717988/1‑101 (MQ=255)
                                                                                          cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             <  1:1698091/101‑1 (MQ=255)
                                                                                           aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            >  2:2297465/7‑101 (MQ=255)
                                                                                           aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg            >  1:3200159/1‑101 (MQ=255)
                                                                                            ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg           >  2:912000/1‑101 (MQ=255)
                                                                                            ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg           >  1:1530267/1‑101 (MQ=255)
                                                                                             gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCGGTCACCACGATGGCGGCGGGGCCCGCCGGGCGGCCGGTGTGGc          >  1:3127709/1‑101 (MQ=255)
                                                                                              aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa         >  1:3602654/4‑101 (MQ=255)
                                                                                              aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         >  1:2891683/1‑101 (MQ=255)
                                                                                               aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt        >  1:865917/3‑101 (MQ=255)
                                                                                               aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt        >  2:4487364/3‑101 (MQ=255)
                                                                                               aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt        <  1:3302786/99‑1 (MQ=255)
                                                                                               aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt        <  1:3532706/99‑1 (MQ=255)
                                                                                                cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  1:1004608/101‑1 (MQ=255)
                                                                                                cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  1:2562710/101‑1 (MQ=255)
                                                                                                cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  2:212902/101‑1 (MQ=255)
                                                                                                aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt       >  1:209816/2‑101 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      >  1:3969956/1‑101 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      >  1:814666/1‑101 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:1514633/101‑1 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:1908911/101‑1 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:2738952/101‑1 (MQ=255)
                                                                                                 aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  1:4424977/101‑1 (MQ=255)
                                                                                                 aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  2:380899/101‑1 (MQ=255)
                                                                                                  tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     >  1:141335/1‑101 (MQ=255)
                                                                                                   gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa    <  2:4044752/101‑1 (MQ=255)
                                                                                                     gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  <  2:2023793/101‑1 (MQ=255)
                                                                                                     gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  <  1:2127644/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG