Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,445 G→A 14.7% A4T (GCA→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4450G→A14.7% 70.6 ‑0.2 ‑0.0217A4T (GCA→ACA) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/18):  ref base (87/98):  total (101/116)
Fisher's exact test strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 229 total aligned reads displayed.

GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155345‑2155545
                                                                                                     |                                                                                                    
gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                      >  1:3533461/1‑101 (MQ=255)
cccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                      <  1:4008100/99‑1 (MQ=255)
 ccccGGCGGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCACCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                     <  1:713962/100‑1 (MQ=255)
 ccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCCCCGCCCCCGTCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                     <  1:280804/100‑1 (MQ=255)
  cccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    >  2:1608601/1‑101 (MQ=255)
   ccGGCCGCGCTCGCCCTCTCCTTCTGACGCCCTGGGGCGCGGTCCCCTTCACCGCCCG.CGGGCGGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                   <  2:4350952/101‑1 (MQ=255)
     ggCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                 >  1:2481544/1‑101 (MQ=255)
     ggCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                 >  2:2901191/1‑101 (MQ=255)
           gtccGCCCTCCCCTCCTGATCCCCTCGGGCCCCGTCCCCTTCCCCGCCCGCCGGGCGC.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           <  2:2107872/98‑1 (MQ=255)
           ggccccccTCCCCTTCTGATGCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           <  1:1381551/96‑1 (MQ=255)
           ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           >  2:3868530/1‑101 (MQ=255)
            gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          >  2:855998/1‑101 (MQ=255)
               ccccTCGCCTTCTGACGCCCTGGGGCGCCGCCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                       <  2:1020164/100‑1 (MQ=255)
               ccccTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                       <  1:1480432/100‑1 (MQ=255)
                cccTCCCCTTCTGATCCCCTGGGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                      <  1:1833372/101‑1 (MQ=255)
                 ctccACCTTCTGACGCCCCCGGCCGCCGCCACCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                     <  2:2869250/98‑1 (MQ=255)
                 ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                     >  2:1391675/1‑101 (MQ=255)
                  cTCCCCTTCTGATGCCCGGGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                    <  1:2741721/101‑1 (MQ=255)
                   tCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                   >  1:1070119/1‑101 (MQ=255)
                   cccccTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                   <  1:3136455/98‑1 (MQ=255)
                    cACCTCCTGATGCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                  <  1:3093597/101‑1 (MQ=255)
                      ccTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgt                                                                                >  1:2177572/1‑101 (MQ=255)
                       cTTCTGATCCCCGCGGGCGCCGCCCCCGTCCCCCCCCCCCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                               <  1:3381350/101‑1 (MQ=255)
                           tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAtc                                                                           >  2:1230738/1‑100 (MQ=255)
                           tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                           >  1:1145837/1‑101 (MQ=255)
                            gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                          >  2:2497233/1‑101 (MQ=255)
                            gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCATCGTAGAGCTCGTCGATGc                                                                          >  2:1554164/1‑101 (MQ=255)
                            gATCCCCTGGGGCGCCGTCCCCGCCCCCCCCCGCCGGGGGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                          <  1:3824628/101‑1 (MQ=255)
                             aTGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                         >  1:1332377/1‑101 (MQ=255)
                               ccccTGGGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                       <  1:1257473/100‑1 (MQ=255)
                                cccCCGGGCGCCGTCCCCGCCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                      <  1:2329923/101‑1 (MQ=255)
                                 cccggggCGCCGCCCCCGTCCCCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCt                                                                     <  1:3193605/97‑1 (MQ=255)
                                  cTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTg                                                                    >  2:3028453/1‑101 (MQ=255)
                                   tCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                   >  2:2768211/1‑101 (MQ=255)
                                    ggggCCCCGCCCCCGCCCCCCCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                  <  1:585824/100‑1 (MQ=255)
                                         gcCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                             >  2:3099224/1‑101 (MQ=255)
                                            gcccccgcccccgcccgcCGGGCGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                          <  2:1272712/91‑1 (MQ=255)
                                             tcaccgtcaccgCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                         >  1:4281422/1‑101 (MQ=255)
                                               accgtcaccgCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                       >  2:440862/1‑101 (MQ=255)
                                                ccgcccccccccgcgGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                      <  1:2673994/92‑1 (MQ=255)
                                                      ccccccgccGGGCAA.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                                <  1:3099424/98‑1 (MQ=255)
                                                         ccggcggggCGA.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                             <  2:1881175/95‑1 (MQ=255)
                                                              ggggGGG.AAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                        <  1:856072/100‑1 (MQ=255)
                                                              cgGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                        >  1:447258/1‑101 (MQ=255)
                                                              cgGGCGA.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                        >  2:1139421/1‑101 (MQ=255)
                                                                ggcgg.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                      >  1:4528714/1‑101 (MQ=255)
                                                                  gaaaaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGgcggcggcg                                     <  2:4281175/96‑1 (MQ=255)
                                                                  cgg.cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                    >  1:2489939/1‑101 (MQ=255)
                                                                    ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                   <  2:1969897/98‑1 (MQ=255)
                                                                       aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGc                                >  1:1282401/1‑101 (MQ=255)
                                                                       aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                >  1:3081161/1‑101 (MQ=255)
                                                                           cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                            >  1:198436/1‑101 (MQ=255)
                                                                           cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                            >  2:845873/1‑101 (MQ=255)
                                                                            gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                           >  1:3699837/1‑101 (MQ=255)
                                                                              gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                         <  2:4013712/101‑1 (MQ=255)
                                                                              gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                         <  1:3511273/101‑1 (MQ=255)
                                                                                 aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                      >  1:3223404/1‑101 (MQ=255)
                                                                                  gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                     >  1:2402675/1‑101 (MQ=255)
                                                                                  gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                     >  1:294636/1‑101 (MQ=255)
                                                                                    gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                   >  1:2128595/1‑101 (MQ=255)
                                                                                    gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                   >  2:3332150/1‑101 (MQ=255)
                                                                                    gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                   >  2:3717988/1‑101 (MQ=255)
                                                                                       ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                >  2:912000/1‑101 (MQ=255)
                                                                                       ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                >  1:1530267/1‑101 (MQ=255)
                                                                                        gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc               >  2:269132/1‑101 (MQ=255)
                                                                                          tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAt             <  2:3080973/101‑1 (MQ=255)
                                                                                          aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             >  1:4257365/3‑101 (MQ=255)
                                                                                          aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             <  2:4196927/99‑1 (MQ=255)
                                                                                          aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             <  2:3139915/99‑1 (MQ=255)
                                                                                          aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             >  1:865917/3‑101 (MQ=255)
                                                                                          aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             <  2:4481525/99‑1 (MQ=255)
                                                                                           cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  2:4666915/101‑1 (MQ=255)
                                                                                           cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  1:1004608/101‑1 (MQ=255)
                                                                                           aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt            >  2:74977/2‑101 (MQ=255)
                                                                                           aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt            >  1:209816/2‑101 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           >  1:814666/1‑101 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           >  1:695011/1‑101 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           <  1:652988/101‑1 (MQ=255)
                                                                                            aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  2:2820118/101‑1 (MQ=255)
                                                                                            aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           >  2:4513654/1‑101 (MQ=255)
                                                                                            aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  1:4424977/101‑1 (MQ=255)
                                                                                            aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  2:380899/101‑1 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          >  1:141335/1‑101 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          <  1:1554265/101‑1 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          <  2:4528461/101‑1 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          >  1:4624211/1‑101 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          >  2:2178720/1‑101 (MQ=255)
                                                                                               accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc        >  2:3547641/3‑101 (MQ=255)
                                                                                               accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc        <  1:1086368/99‑1 (MQ=255)
                                                                                                ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc       <  1:2026699/100‑1 (MQ=255)
                                                                                                 cgccgCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg      >  2:1726547/5‑101 (MQ=255)
                                                                                                 cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg      <  1:1241171/101‑1 (MQ=255)
                                                                                                 cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg      <  1:3624467/101‑1 (MQ=255)
                                                                                                 cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg      <  1:406283/101‑1 (MQ=255)
                                                                                                  gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa     >  1:2308134/1‑101 (MQ=255)
                                                                                                  gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa     <  2:1839365/101‑1 (MQ=255)
                                                                                                    tGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgg   <  2:2019331/101‑1 (MQ=255)
                                                                                                     gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAggg  >  1:1030099/1‑100 (MQ=255)
                                                                                                     gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgga  >  2:2302770/1‑100 (MQ=255)
                                                                                                     gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGt  <  2:4609358/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGG.CAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155345‑2155545

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG