Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,576 C→T 37.2% R47R (CGC→CGT)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5760C→T37.2% 274.3 ‑0.1 0.0266R47R (CGC→CGT) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (48/51):  ref base (83/84):  total (131/135)
Fisher's exact test strand distribution p-value = 8.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCG  >  NC_014034/2155477‑2155676
                                                                                                   |                                                                                                    
 tGGGGCGCGGCCGTCACCACGATGGCGGCGGCGCTCGCGGGGCGGCTGATGTGGCATTCGACCGAAGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGa                                                                                                    <  2:2880241/101‑1 (MQ=255)
  ggggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcggg                                                                                                   >  2:727450/1‑99 (MQ=255)
  ggggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTCAGCCGCGAg                                                                                                   >  2:4487214/1‑101 (MQ=255)
   gggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgc                                                                                                  >  2:1193033/1‑101 (MQ=255)
    ggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgct                                                                                                 >  2:4023626/1‑101 (MQ=255)
      cggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgc                                                                                               >  1:2163537/1‑101 (MQ=255)
       ggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgct                                                                                              >  2:295599/1‑101 (MQ=255)
          gcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTg                                                                                           <  2:559203/101‑1 (MQ=255)
                acgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCt                                                                                     >  1:2640599/4‑101 (MQ=255)
                  cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                                                   >  1:1213592/1‑101 (MQ=255)
                     gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccgg                                                                                >  2:659676/1‑99 (MQ=255)
                     gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccgg                                                                                >  1:1427178/1‑99 (MQ=255)
                     gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGccgg                                                                                >  1:1010274/1‑99 (MQ=255)
                       tGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGtg                                                                              >  1:3152410/1‑100 (MQ=255)
                       tGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGCCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTc                                                                              <  1:1614718/101‑1 (MQ=255)
                       tGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTc                                                                              >  1:1397038/1‑101 (MQ=255)
                              ggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCg                                                                       <  2:1677870/101‑1 (MQ=255)
                                 gcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCa                                                                    <  2:3476101/101‑1 (MQ=255)
                                  ctggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCAt                                                                   <  2:2933650/98‑1 (MQ=255)
                                   tggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATg                                                                  >  1:3367674/3‑101 (MQ=255)
                                   tCGCGGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                                  <  2:241114/101‑1 (MQ=255)
                                    ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGTGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                                                 <  1:3406001/100‑1 (MQ=255)
                                     gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                                >  1:4343150/1‑101 (MQ=255)
                                     gCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGc                                                                <  1:250261/101‑1 (MQ=255)
                                     gCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGc                                                                <  2:4247865/101‑1 (MQ=255)
                                        gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                             >  2:1538253/1‑101 (MQ=255)
                                        gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                             >  1:2897918/1‑101 (MQ=255)
                                        gggCGCCTGATGTGGCATTCGACCGAGGGGCGGGCGCGGCGGGGCCGGATCTTGGGGCGTGCACTGCTGTGGGAGCTGCCGGTGGCCGGGGGCATGGCGat                                                             >  1:4394083/1‑101 (MQ=255)
                                         ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                                            <  2:814593/101‑1 (MQ=255)
                                         ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                                            >  1:4169417/1‑101 (MQ=255)
                                         ggcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatc                                                            >  1:1651847/1‑101 (MQ=255)
                                           cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                                          <  2:4377413/101‑1 (MQ=255)
                                           cggcTGAGGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                                          <  2:2369389/101‑1 (MQ=255)
                                           cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                          <  1:2051056/98‑1 (MQ=255)
                                             ccTGATGTGGCATTCGACGGAGGTGCGGGGGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                                        <  2:3186110/100‑1 (MQ=255)
                                               tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                                      >  1:4561136/1‑101 (MQ=255)
                                                gATGTTGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                                     <  2:1726189/101‑1 (MQ=255)
                                                gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                                     >  2:4692267/1‑101 (MQ=255)
                                                  tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                                   >  1:649018/1‑101 (MQ=255)
                                                  tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                                   <  2:3803461/101‑1 (MQ=255)
                                                    tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGc                                                 >  2:3370734/1‑101 (MQ=255)
                                                     ggCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTAGGCATGGCGATCATCGGCGAGGCg                                                >  2:2678393/1‑101 (MQ=255)
                                                        aTTCGACTGAGGTGCGCGGGCGGCGGCGCGGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTCCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                             <  1:1874143/101‑1 (MQ=255)
                                                        aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                             >  1:1154504/1‑101 (MQ=255)
                                                        aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                             <  2:1253427/101‑1 (MQ=255)
                                                          tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggc                                           <  1:3915794/101‑1 (MQ=255)
                                                          tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggc                                           <  1:2805243/101‑1 (MQ=255)
                                                          tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                           <  1:223065/101‑1 (MQ=255)
                                                          tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                           <  1:1663765/101‑1 (MQ=255)
                                                           cGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcg                                          <  2:2507621/101‑1 (MQ=255)
                                                           cGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcg                                          >  2:182573/1‑101 (MQ=255)
                                                            gACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcgg                                         <  2:2458169/101‑1 (MQ=255)
                                                             aCCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcggc                                        <  1:1809695/101‑1 (MQ=255)
                                                               cGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                                      >  1:1856340/1‑101 (MQ=255)
                                                                gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                     >  1:327321/1‑101 (MQ=255)
                                                                gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                     >  1:3571895/1‑101 (MQ=255)
                                                                gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                     <  2:3969714/101‑1 (MQ=255)
                                                                 aGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                    >  2:4160841/1‑101 (MQ=255)
                                                                 aGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                    >  2:354478/1‑101 (MQ=255)
                                                                 aGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                    <  2:892420/101‑1 (MQ=255)
                                                                 aGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                    >  1:1774681/1‑101 (MQ=255)
                                                                  ggTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAtt                                   <  2:1901319/101‑1 (MQ=255)
                                                                   gcgCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                                  <  1:1112169/99‑1 (MQ=255)
                                                                   gTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                                  <  2:1915672/101‑1 (MQ=255)
                                                                   gTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                                  <  1:4023868/101‑1 (MQ=255)
                                                                    tGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTc                                 >  1:2439950/1‑101 (MQ=255)
                                                                    tGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAAGCGGTGGCGGCTTATTTc                                 >  2:6030/1‑101 (MQ=255)
                                                                    tGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTc                                 <  1:4601876/101‑1 (MQ=255)
                                                                    ggCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTc                                 <  2:4289322/100‑1 (MQ=255)
                                                                     gCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                                <  2:3598303/101‑1 (MQ=255)
                                                                     gCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                                <  1:348454/101‑1 (MQ=255)
                                                                      cGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGa                               <  1:1594567/101‑1 (MQ=255)
                                                                      cGGCCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGa                               <  2:2611795/101‑1 (MQ=255)
                                                                         gcgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCACCGGCGAGGCGGTGGCGGCTTATTTCGAGCt                            <  1:4224377/101‑1 (MQ=255)
                                                                            cgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg                         <  2:4211850/101‑1 (MQ=255)
                                                                            cgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg                         >  1:3638364/1‑101 (MQ=255)
                                                                            cgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCgg                         >  2:2915418/1‑101 (MQ=255)
                                                                              cAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGcc                       >  1:472025/1‑101 (MQ=255)
                                                                              cAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGcc                       >  1:3436347/1‑101 (MQ=255)
                                                                                aGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCgg                     <  2:3638135/101‑1 (MQ=255)
                                                                                   gccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGGcc                  >  1:854880/1‑101 (MQ=255)
                                                                                   gccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAcc                  <  1:960097/101‑1 (MQ=255)
                                                                                    ccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCg                 <  2:1641400/101‑1 (MQ=255)
                                                                                    ccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCg                 <  1:3795881/101‑1 (MQ=255)
                                                                                      gcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTNNNNNCTTATTTCGAGCTCGGCCGGACCGTg               >  1:3120089/1‑101 (MQ=255)
                                                                                        ttgcTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac             >  1:2714523/4‑101 (MQ=255)
                                                                                        ttCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac             >  1:1450908/1‑101 (MQ=255)
                                                                                        ttCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac             >  2:3127594/1‑101 (MQ=255)
                                                                                         tCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGGGacc            >  2:3876421/1‑101 (MQ=255)
                                                                                          ccTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacca           <  2:2101661/101‑1 (MQ=255)
                                                                                           cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac          >  1:818984/1‑101 (MQ=255)
                                                                                           cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGAATGGAGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac          >  2:4101895/1‑101 (MQ=255)
                                                                                            tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc         <  1:2957771/101‑1 (MQ=255)
                                                                                            tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc         <  2:1379617/101‑1 (MQ=255)
                                                                                               gCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGc      <  2:327287/101‑1 (MQ=255)
                                                                                                 cgcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgt    >  2:4543386/1‑101 (MQ=255)
                                                                                                 cgcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgt    >  2:1118146/1‑101 (MQ=255)
                                                                                                 cgcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGCGACCACCGGcgt    >  1:1499932/1‑101 (MQ=255)
                                                                                                  gcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtc   <  2:1856228/101‑1 (MQ=255)
                                                                                                  gcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtc   <  1:3063079/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCG  >  NC_014034/2155477‑2155676

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG