Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,647 A→G 35.2% E71G (GAG→GGG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6470A→G35.2% 0.0 ‑0.3 ‑1.6335E71G (GAG→GGG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (66/52):  ref base (116/101):  total (182/153)
Fisher's exact test strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.37e-02

Only 100 of 359 total aligned reads displayed.

CGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAG  >  NC_014034/2155547‑2155747
                                                                                                    |                                                                                                    
 gggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAg                                                                                                     >  2:509843/1‑101 (MQ=255)
       gcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc                                                                                               >  2:2938642/1‑101 (MQ=255)
       gcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc                                                                                               >  2:635977/1‑101 (MQ=255)
       gcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGGGGCGGCTTATTTCGAGCTCGGc                                                                                               >  1:83349/1‑101 (MQ=255)
         aaGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCg                                                                                             <  2:2891507/101‑1 (MQ=255)
         aaGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCg                                                                                             >  1:3949556/1‑101 (MQ=255)
              ccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCg                                                                                        <  1:2492996/101‑1 (MQ=255)
                gcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTNNNNNCTTATTTCGAGCTCGGCCGGACCGTg                                                                                      >  1:3120089/1‑101 (MQ=255)
                    ccTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacca                                                                                  <  1:3028641/101‑1 (MQ=255)
                    ccTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacca                                                                                  <  2:2101661/101‑1 (MQ=255)
                     cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                                                                                 >  2:4238424/1‑101 (MQ=255)
                     cTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                                                                                 >  1:3415581/1‑101 (MQ=255)
                      tcgggggtgAGCTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGaccacc                                                                                <  1:3613290/93‑1 (MQ=255)
                      tGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc                                                                                >  2:263144/1‑101 (MQ=255)
                        gggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCgg                                                                              >  1:867542/10‑101 (MQ=255)
                        ggCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCgg                                                                              <  1:1230828/100‑1 (MQ=255)
                        aGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCgg                                                                              <  1:4361260/101‑1 (MQ=255)
                         ggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGc                                                                             >  1:2427300/9‑101 (MQ=255)
                           cgcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGCGACCACCGGcgt                                                                           >  1:1499932/1‑101 (MQ=255)
                            gcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtc                                                                          <  1:2768376/101‑1 (MQ=255)
                             tgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGcgtcg                                                                         >  2:1624399/5‑101 (MQ=255)
                              gAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtcgt                                                                        >  1:1327328/1‑101 (MQ=255)
                               aactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCgtcgtc                                                                       <  2:3047019/99‑1 (MQ=255)
                               aGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCAGGACCGTGACCACCGGCgtcgtc                                                                       <  1:2253312/101‑1 (MQ=255)
                                gctgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGtcgtcg                                                                      >  1:3759538/1‑101 (MQ=255)
                                 ctgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGc                                                                     >  1:2560923/1‑101 (MQ=255)
                                  tgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGcc                                                                    >  2:3432716/1‑100 (MQ=255)
                                  tgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCa                                                                    >  2:4517989/1‑101 (MQ=255)
                                   gctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGcct                                                                   >  2:2450695/1‑99 (MQ=255)
                                    ctgtGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGGGACCACCGGCGTCGTCGCAAc                                                                  <  2:3247430/98‑1 (MQ=255)
                                       tGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCt                                                               >  1:4587762/1‑101 (MQ=255)
                                       tGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCt                                                               >  2:1879774/1‑101 (MQ=255)
                                        gggAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTc                                                              <  1:1660905/101‑1 (MQ=255)
                                        gggAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTc                                                              <  1:3876663/101‑1 (MQ=255)
                                          gAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGCCGCTGACCACCGGCGGCGTCGCCTCGCTCGc                                                            >  2:1128292/1‑101 (MQ=255)
                                           aGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGcc                                                           >  1:973569/1‑101 (MQ=255)
                                            gCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCt                                                          <  1:3273881/101‑1 (MQ=255)
                                             cTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTa                                                         <  2:3105339/101‑1 (MQ=255)
                                              tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                        <  2:3152210/101‑2 (MQ=255)
                                              tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                        >  2:180102/1‑100 (MQ=255)
                                              tGCCCGTCGCGGGCGGCATGGCGATCAGCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                        <  1:1410153/101‑1 (MQ=255)
                                                  ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                    >  2:1091566/11‑101 (MQ=255)
                                                  ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                    >  1:4180757/11‑101 (MQ=255)
                                                  ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                    <  2:219885/91‑1 (MQ=255)
                                                  cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                    >  1:3563717/1‑101 (MQ=255)
                                                  cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                    <  1:1726652/101‑1 (MQ=255)
                                                   gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                   >  2:1946771/10‑101 (MQ=255)
                                                   gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                   >  1:539954/10‑101 (MQ=255)
                                                      gcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCCGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGccc                                                <  1:4016987/101‑1 (MQ=255)
                                                      gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                <  1:347462/95‑1 (MQ=255)
                                                      gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                <  2:3384022/95‑1 (MQ=255)
                                                      gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                <  1:1128378/95‑1 (MQ=255)
                                                       cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                               >  2:2653673/1‑101 (MQ=255)
                                                       cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                               >  2:2535827/1‑101 (MQ=255)
                                                       ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                               >  2:4081941/6‑101 (MQ=255)
                                                        cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                              >  1:4226222/5‑101 (MQ=255)
                                                         gTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcg                                             >  2:4270621/1‑101 (MQ=255)
                                                          tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                            >  2:2168304/3‑101 (MQ=255)
                                                          tCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcgc                                            <  2:1339598/101‑1 (MQ=255)
                                                           gggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgcg                                           <  2:1283382/100‑1 (MQ=255)
                                                           cGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcgcg                                           >  2:4149075/1‑101 (MQ=255)
                                                            ggCCTGGCGGTCATCGGCGAGGCGGTGGCGGCTTCTTTCGAGCTCGCCCGGACCCTGGCCCCCGGCGGCGTCGCATCGCTCGCCTATTTCGTCCcgttcgg                                          >  2:687042/1‑96 (MQ=255)
                                                            ggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcgg                                          <  1:1846609/101‑1 (MQ=255)
                                                             gCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggg                                         <  2:720151/101‑2 (MQ=255)
                                                                 ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                     <  2:83723/101‑1 (MQ=255)
                                                                  gCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCa                                    <  2:3571676/101‑1 (MQ=255)
                                                                     atcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCCGGGccggggg                                 >  1:4440918/1‑96 (MQ=255)
                                                                     atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAggg                                 >  2:1750180/1‑100 (MQ=255)
                                                                        atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgct                              <  2:1178874/101‑3 (MQ=255)
                                                                        atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                              <  1:23817/101‑1 (MQ=255)
                                                                        atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGGGgc                              >  1:89613/1‑101 (MQ=255)
                                                                          cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgc                            <  2:2053983/101‑5 (MQ=255)
                                                                           ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                           <  2:4294589/101‑6 (MQ=255)
                                                                           gccGAGGCGGGAGCAGCTTATTTCGAGCTCGGCCGCTCCGTGACCACCGGCGTAGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                           <  2:318869/99‑1 (MQ=255)
                                                                            gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                          <  1:1583119/101‑1 (MQ=255)
                                                                            gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                          <  1:3156605/101‑1 (MQ=255)
                                                                             cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACAGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGa                         >  2:3874538/1‑101 (MQ=255)
                                                                              gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGNGCCCAGGTGGCGCTGAc                        >  2:2230522/1‑101 (MQ=255)
                                                                               aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                       <  2:3949314/101‑1 (MQ=255)
                                                                               aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGCGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                       >  1:2478914/1‑101 (MQ=255)
                                                                                ggCTGTGGCGGCTTCTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                      <  2:1447639/101‑1 (MQ=255)
                                                                                ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                      <  1:454355/101‑1 (MQ=255)
                                                                                  cGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                    >  1:3824896/1‑96 (MQ=255)
                                                                                   ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                   >  1:803760/1‑95 (MQ=255)
                                                                                   ggTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCggg                   >  2:783932/1‑101 (MQ=255)
                                                                                        cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc              <  1:2937292/101‑1 (MQ=255)
                                                                                        cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc              >  2:4444934/1‑101 (MQ=255)
                                                                                         ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct             >  2:1417904/1‑101 (MQ=255)
                                                                                          gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGGCCCGGGTcttctt            <  2:2408671/101‑1 (MQ=255)
                                                                                            ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc          >  2:2837898/1‑101 (MQ=255)
                                                                                            ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc          <  1:2085383/101‑1 (MQ=255)
                                                                                            ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGCGGCGCTGACCCGGGTCTTCTTTc          >  1:2853033/1‑101 (MQ=255)
                                                                                              aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac        >  2:2772549/1‑101 (MQ=255)
                                                                                               tttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca       >  2:2704183/1‑101 (MQ=255)
                                                                                                ttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaa      >  2:4119661/1‑101 (MQ=255)
                                                                                                  cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca    <  2:4585256/101‑1 (MQ=255)
                                                                                                  cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca    <  2:4623958/101‑1 (MQ=255)
                                                                                                  cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca    <  1:2147219/101‑1 (MQ=255)
                                                                                                  cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca    >  2:3656094/1‑101 (MQ=255)
                                                                                                    aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg  <  1:479691/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAG  >  NC_014034/2155547‑2155747

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG