Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,693 T→C 24.5% Y86Y (TAT→TAC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6930T→C24.5% 199.9 ‑0.4 ‑0.0297Y86Y (TAT→TAC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (41/32):  ref base (118/106):  total (159/138)
Fisher's exact test strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.04e-01

Only 100 of 315 total aligned reads displayed.

TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTC  >  NC_014034/2155593‑2155793
                                                                                                    |                                                                                                    
tGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                                                                      <  1:798930/101‑1 (MQ=255)
tGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                                                                      >  2:4399835/1‑101 (MQ=255)
 gCCCGACGCGGTCGCCATGGCCATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTGGCCTAtc                                                                                                     <  2:3588512/101‑1 (MQ=255)
  cccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtct                                                                                                    <  2:1213502/101‑1 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                  <  2:219885/91‑1 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                  >  2:1091566/11‑101 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCCGTGGCGACTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                  <  2:1160137/91‑1 (MQ=255)
    cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                                                                  >  1:3563717/1‑101 (MQ=255)
     gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                                                 >  1:3368445/10‑101 (MQ=255)
        gcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGccc                                                                                              >  1:999789/1‑101 (MQ=255)
        gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                              <  1:1128378/95‑1 (MQ=255)
         cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                             >  2:2653673/1‑101 (MQ=255)
         cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                             <  1:492658/101‑1 (MQ=255)
         cgGGCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                             <  1:2490358/101‑1 (MQ=255)
         ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                                                                             >  2:4081941/6‑101 (MQ=255)
          cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                                                            >  1:4226222/5‑101 (MQ=255)
          cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                                                            >  1:2457439/5‑101 (MQ=255)
          cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTAGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                                                            >  2:2421059/5‑101 (MQ=255)
            tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                                                                          <  2:2327046/99‑1 (MQ=255)
            tCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcgc                                                                                          <  2:1339598/101‑1 (MQ=255)
            tCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTAGCCTATCTCGGCCcgcgc                                                                                          >  1:4364789/1‑101 (MQ=255)
             gggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgcg                                                                                         <  2:1283382/100‑1 (MQ=255)
             cGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcgcg                                                                                         >  2:4149075/1‑101 (MQ=255)
              ggCCTGGCGGTCATCGGCGAGGCGGTGGCGGCTTCTTTCGAGCTCGCCCGGACCCTGGCCCCCGGCGGCGTCGCATCGCTCGCCTATTTCGTCCcgttcgg                                                                                        >  2:687042/1‑96 (MQ=255)
               gCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggg                                                                                       <  2:720151/101‑2 (MQ=255)
                cATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcgggg                                                                                      >  2:2384341/1‑99 (MQ=255)
                 aTGGCGATGATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGgggc                                                                                     <  2:4617776/101‑1 (MQ=255)
                  tGGCGATCGTCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGcc                                                                                    <  2:4271972/101‑1 (MQ=255)
                  tGGCGATCATCGTCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGcc                                                                                    <  2:3229320/101‑1 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccg                                                                                   >  1:869316/1‑100 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCCCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                                                   <  1:2818056/101‑1 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCCGGGccggggg                                                                               >  1:4440918/1‑96 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAggg                                                                               >  1:1447642/1‑100 (MQ=255)
                         catcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccgnnnggn                                                                             >  1:1559334/1‑94 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGCGGTgct                                                                            >  1:3805516/1‑99 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                         >  2:317360/1‑96 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                         >  2:64054/1‑96 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                         >  2:2781430/1‑96 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                         <  2:4294589/101‑6 (MQ=255)
                               cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCCAGGTGCTGCTgg                                                                       >  2:3386462/1‑100 (MQ=255)
                               cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACAGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGa                                                                       >  2:3874538/1‑101 (MQ=255)
                                gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCCCGGGGCCGAGGGGCGGCTGat                                                                      >  2:1962703/1‑100 (MQ=255)
                                 aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                                                     <  2:3949314/101‑1 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                    <  1:454355/101‑1 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGCGGCCGAGGTGCTGCTGatgagca                                                                 >  1:3128561/1‑95 (MQ=255)
                                      gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                                >  2:1900952/1‑94 (MQ=255)
                                       tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatc                                                               <  2:2897742/101‑9 (MQ=255)
                                           ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                           >  2:1417904/1‑101 (MQ=255)
                                           ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                           >  2:4657224/1‑101 (MQ=255)
                                             cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                         >  1:3851518/1‑101 (MQ=255)
                                             cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                         >  1:3269493/1‑101 (MQ=255)
                                              ttATTTCGAGCTCGGCCGGACCGTGACCCCCGGCGTCGTCTCAACGCTCGCCTCTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                        <  2:777092/101‑1 (MQ=255)
                                              ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                        <  1:2915594/101‑1 (MQ=255)
                                               tATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                                                       <  2:854806/101‑1 (MQ=255)
                                                  ttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaa                                                    >  2:4119661/1‑101 (MQ=255)
                                                   tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                   <  1:2207643/101‑1 (MQ=255)
                                                    cGAGCTCGTCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca                                                  <  1:2302903/101‑1 (MQ=255)
                                                    cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca                                                  <  1:2147219/101‑1 (MQ=255)
                                                      aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg                                                >  1:721208/1‑101 (MQ=255)
                                                          cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                            <  1:295633/101‑1 (MQ=255)
                                                             ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                         <  1:192320/101‑1 (MQ=255)
                                                             ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                         <  1:1405914/101‑1 (MQ=255)
                                                              cGGACCGTGAGCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTg                                        >  1:3245629/1‑101 (MQ=255)
                                                              cGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTg                                        <  2:286479/101‑1 (MQ=255)
                                                              cGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTg                                        >  1:1796097/1‑101 (MQ=255)
                                                               ggACCGTGACCACGGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                       >  1:3177275/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                       >  1:2508692/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                       >  2:1433875/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                       >  2:212694/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                       >  2:3076059/1‑101 (MQ=255)
                                                                ggccttgtccaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                      <  2:1530166/93‑1 (MQ=255)
                                                                gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                      >  1:343663/1‑101 (MQ=255)
                                                                    gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                                  >  2:3795365/1‑101 (MQ=255)
                                                                    gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                                  <  2:4753519/101‑1 (MQ=255)
                                                                    gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                                  <  2:1604767/101‑1 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCGCGGGCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                 <  1:4064569/101‑1 (MQ=255)
                                                                        ccaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacgac                              >  2:2826453/1‑101 (MQ=255)
                                                                         caccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACa                             <  2:2491769/101‑1 (MQ=255)
                                                                           ccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGa                           <  2:1795988/101‑1 (MQ=255)
                                                                            cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                          >  1:3929696/1‑101 (MQ=255)
                                                                            cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                          <  2:3563498/101‑1 (MQ=255)
                                                                            cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                          <  1:1292248/101‑1 (MQ=255)
                                                                              gCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTg                        <  2:4560883/101‑1 (MQ=255)
                                                                               cgtcgtCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGGCGTGAGATGACGACAGACTgg                       >  1:6636/1‑101 (MQ=255)
                                                                                  cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGGGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                    >  1:4717080/1‑101 (MQ=255)
                                                                                  cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                    >  2:3758939/1‑101 (MQ=255)
                                                                                    tcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGc                  <  2:2714369/101‑1 (MQ=255)
                                                                                     cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc                 <  1:2704337/101‑1 (MQ=255)
                                                                                      gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcg                >  1:1712912/1‑101 (MQ=255)
                                                                                        aaCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgcg              >  2:2516965/1‑101 (MQ=255)
                                                                                            cTCGCCTATCTCGGCCCCCGCGGCGCCCAGGTGGGGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGc          >  1:1641954/1‑101 (MQ=255)
                                                                                              cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCCGCTg        >  2:2624562/1‑101 (MQ=255)
                                                                                                ccTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCccgcccccgcg      >  1:1236041/1‑94 (MQ=255)
                                                                                                ccTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCg      <  2:343629/101‑1 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCGGCTGCgg     >  2:3310180/1‑101 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGAGCGCAGcggagc     >  2:821241/1‑96 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCCGCGGCgg     >  1:4586379/1‑101 (MQ=255)
                                                                                                  tATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCCGCggg    >  1:100009/1‑100 (MQ=255)
                                                                                                  tATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGCGCCGCGCAGCTGCGGc    <  1:4149321/101‑1 (MQ=255)
                                                                                                    tctcGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTc  <  2:1705369/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTC  >  NC_014034/2155593‑2155793

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG