Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,213 G→A 100% P55L (CCC→CTC)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2130G→A100.0% 931.6 240P55L (CCC→CTC) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (137/103):  ref base (0/0):  total (137/103)

Only 100 of 246 total aligned reads displayed.

GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305
                                                                                                  |                                                                                                 
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:3530218/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:1797347/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:583328/1‑101 (MQ=255)
   tGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAcc                                                                                              >  1:1830179/1‑101 (MQ=255)
    gATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCa                                                                                             >  1:1387083/1‑101 (MQ=255)
     ctCAAAATCCCCTTCACCGCGTCCGCGATGCACCTTTCGACCGCCGCGATCTGCGATTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGAACCAg                                                                                            <  2:1743793/100‑1 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           >  1:2783805/1‑101 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           <  1:3218209/101‑1 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           >  1:398906/1‑101 (MQ=255)
       cAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcc                                                                                          >  1:1553625/1‑101 (MQ=255)
       cAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcc                                                                                          <  1:848478/101‑1 (MQ=255)
         aaaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcccc                                                                                        >  2:881091/1‑101 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCGCGTTCCGACCGCCGCGTTCTGCGTTTCCGCATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  2:1867449/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      >  1:2360217/1‑101 (MQ=255)
            tCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtc                                                                                     >  1:3135815/1‑101 (MQ=255)
            tCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtc                                                                                     >  1:926428/1‑101 (MQ=255)
            tCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtc                                                                                     <  2:3636846/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    <  2:2094745/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  1:3567142/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  2:3269508/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTCGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  1:4173692/1‑101 (MQ=255)
               ccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCg                                                                                  <  1:2410990/101‑1 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 <  1:2817782/101‑1 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 <  2:4449670/101‑1 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                >  2:90125/1‑101 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                >  1:1781909/1‑101 (MQ=255)
                  ttGCCCCGCCCGCGATGCTGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               <  2:3958116/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  2:2929517/1‑101 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  2:408556/1‑101 (MQ=255)
                   tGCCCCGCCCGCGCTGCAGCTTCCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCg                                                                              <  2:664659/101‑1 (MQ=255)
                   tGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCg                                                                              >  1:4551251/1‑101 (MQ=255)
                     ccccGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGcc                                                                            >  1:4027410/1‑101 (MQ=255)
                      cccgcccgCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCTGTCGCCt                                                                           >  2:4023476/1‑101 (MQ=255)
                         gcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTg                                                                        <  2:3200761/101‑1 (MQ=255)
                             gcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGa                                                                    >  1:4681746/1‑101 (MQ=255)
                             gcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGa                                                                    >  1:2612887/1‑101 (MQ=255)
                             gcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATATTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGa                                                                    >  2:1222414/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:1013609/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  <  1:3786785/101‑1 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  1:1129645/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  2:4355556/101‑1 (MQ=255)
                                  gCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAc                                                               >  1:4522009/1‑101 (MQ=255)
                                   cAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcc                                                              >  1:713299/1‑101 (MQ=255)
                                     gCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcctt                                                            <  1:745306/101‑1 (MQ=255)
                                     gCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcctt                                                            <  2:1584375/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGAGCTTCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCGATCTCGGTCGCCTTTGCCGAAGCCccttc                                                           <  2:4042461/101‑1 (MQ=255)
                                          cGACCGCCGCGATCTGCGTTTCCACATCGCCATCTATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACcttccttc                                                       >  1:4729424/1‑101 (MQ=255)
                                          cGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACcttccttc                                                       >  2:4596148/1‑101 (MQ=255)
                                          cGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACcttccttc                                                       >  1:4246451/1‑101 (MQ=255)
                                           gACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCa                                                      >  2:2009272/1‑101 (MQ=255)
                                            aCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCAt                                                     <  2:1439020/101‑1 (MQ=255)
                                            aCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCCCATAGGCCGAGAGCACAAGCCCCATCTCGGTCGCCTTTCCCAAGGCACcttccttctt                                                     >  2:1681171/1‑99 (MQ=255)
                                             ccgccgCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATc                                                    <  2:2424646/101‑1 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   >  1:165089/1‑101 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   >  1:1621404/1‑101 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  <  2:3086805/101‑1 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  <  2:3594129/101‑1 (MQ=255)
                                                ctgcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  1:3299332/99‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTg                                                 <  1:1222504/101‑1 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAc                                               <  2:4139712/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  1:2236826/1‑101 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  1:3761617/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  2:2595487/1‑101 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  2:1569912/101‑1 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  1:4128157/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            <  2:1847601/101‑1 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           <  2:3826368/101‑1 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          >  2:1902628/1‑101 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          >  1:2982997/1‑101 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          >  2:1359396/1‑101 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          <  1:2626337/101‑1 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  2:538275/101‑2 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        >  2:2766432/1‑99 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        <  2:421926/101‑3 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        <  1:3010128/101‑3 (MQ=255)
                                                         agagccgcacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAGGaaaaag                                        >  2:4234068/8‑99 (MQ=255)
                                                            tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGACGCACCTTCCTTCATCTTGCCGAAAA..agggg                                   >  1:2833371/1‑99 (MQ=255)
                                                             ttCCATATCGCCATCTATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                  <  2:2206895/101‑4 (MQ=255)
                                                             ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                  >  2:3057647/1‑98 (MQ=255)
                                                             ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                  >  1:3114672/1‑98 (MQ=255)
                                                              tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                 <  1:1305507/101‑5 (MQ=255)
                                                               ccccaTCGCCCTCGGTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttg                                <  1:1681276/98‑6 (MQ=255)
                                                                  caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             >  2:1448296/1‑100 (MQ=255)
                                                                     cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcg                          >  2:603949/1‑97 (MQ=255)
                                                                         aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtct                      <  2:2238728/101‑9 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  1:1196099/1‑101 (MQ=255)
                                                                                 tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatc               <  1:1013688/101‑7 (MQ=255)
                                                                                  gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatca              <  1:2899011/101‑8 (MQ=255)
                                                                                    ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcaga            <  1:604554/101‑10 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           <  1:981988/101‑11 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           >  1:1871205/1‑91 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           >  1:2453563/1‑91 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          >  1:866589/1‑101 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          >  2:2660976/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         <  2:2833206/101‑1 (MQ=255)
                                                                                         tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGc        >  1:2089141/1‑101 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      >  1:2749018/1‑101 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      <  2:3464801/101‑1 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      >  1:10590/1‑101 (MQ=255)
                                                                                              cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg   >  2:2697323/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG