Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,209,675 A→G 100% intergenic (+7/‑264) cobO → / → cobQ1 cob(I)yrinic acid a,c‑diamide adenosyltransferase/cobyric acid synthase CobQ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,209,6750A→G100.0% 148.1 197intergenic (+7/‑264)cobO/cobQ1cob(I)yrinic acid a,c‑diamide adenosyltransferase/cobyric acid synthase CobQ
Reads supporting (aligned to +/- strand):  new base (46/102):  ref base (27/22):  total (73/124)
Fisher's exact test strand distribution p-value = 3.57e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.70e-01

Only 100 of 202 total aligned reads displayed.

ATCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAAG.GC.C...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGC  >  NC_014034/2209579‑2209765
                                                                                                |                                                                                                  
aTCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...g                                                                                          <  2:2976908/101‑1 (MQ=255)
aTCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...g                                                                                          <  1:2124526/101‑1 (MQ=255)
  cGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...ggg                                                                                        >  1:976427/1‑101 (MQ=255)
  cGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...ggg                                                                                        >  2:2043604/1‑101 (MQ=255)
   gAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...gggg                                                                                       <  2:4214254/101‑2 (MQ=255)
   gAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...gggg                                                                                       >  1:680028/1‑100 (MQ=255)
   gAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGAAGGCATCAAGGGACAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...gggg                                                                                       >  1:3886438/1‑100 (MQ=255)
    aGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...ggggg                                                                                      <  2:1134395/101‑3 (MQ=255)
     ggcggcCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...GGgggc                                                                                     >  1:731402/1‑101 (MQ=255)
       ctgcCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...GGgggcgc                                                                                   <  2:3803455/99‑3 (MQ=255)
         gcCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...gggggggctg                                                                                 >  1:4347419/1‑94 (MQ=255)
          ccGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C...GGgggcgctgc                                                                                <  1:1922909/101‑6 (MQ=255)
              tCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGccccc                                                                             <  1:880800/101‑1 (MQ=255)
               cTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCg                                                                            >  1:3059756/1‑101 (MQ=255)
               cTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCg                                                                            >  2:1556708/1‑101 (MQ=255)
               cTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCg                                                                            <  1:466755/101‑1 (MQ=255)
                tAGTGACCGAGATGGGTCAGGTGAAGCACCCGTGCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCgg                                                                           <  1:4113646/101‑1 (MQ=255)
                 aGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGAATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGa                                                                          >  2:2751182/1‑101 (MQ=255)
                  gTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGAc                                                                         <  1:841266/101‑1 (MQ=255)
                   tGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGAcc                                                                        <  2:1433237/101‑1 (MQ=255)
                   tGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCCGAcc                                                                        >  2:4075842/1‑101 (MQ=255)
                   tGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGAcc                                                                        <  1:3761196/101‑1 (MQ=255)
                     aCCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGAcccc                                                                      <  2:4675169/101‑1 (MQ=255)
                     aCCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGAcccc                                                                      <  2:2198518/101‑1 (MQ=255)
                       cGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.g                                                                   <  2:3569188/101‑1 (MQ=38)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGCCCCCC.ggg                                                                 >  1:3634938/1‑101 (MQ=255)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.ggg                                                                 >  2:3071140/1‑101 (MQ=38)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.ggg                                                                 <  1:4276099/101‑1 (MQ=38)
                           aTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGat                                                               <  1:452731/101‑1 (MQ=38)
                            tGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGata                                                              >  2:618590/1‑101 (MQ=38)
                            tGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGata                                                              <  1:1556809/101‑1 (MQ=38)
                             gggTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGatat                                                             <  2:3671622/101‑1 (MQ=38)
                              ggTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATAtt                                                            <  2:2552431/101‑1 (MQ=38)
                                  aGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTtgtg                                                        >  2:4609253/1‑101 (MQ=37)
                                  aGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTtgtg                                                        >  2:2331018/1‑101 (MQ=37)
                                   ggTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTgg                                                       <  2:3202914/101‑1 (MQ=37)
                                    gTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGc                                                      <  2:3428055/101‑1 (MQ=37)
                                     tGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCa                                                     <  1:3861056/101‑1 (MQ=37)
                                     tGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCa                                                     <  1:1263577/101‑1 (MQ=37)
                                     tGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCa                                                     <  1:3559147/101‑1 (MQ=37)
                                       aaGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAg                                                   <  1:4435736/101‑1 (MQ=25)
                                        aGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGa                                                  <  2:1039646/101‑1 (MQ=25)
                                          cACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATg                                                >  2:4749259/1‑101 (MQ=25)
                                          cACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATg                                                <  1:338441/101‑1 (MQ=25)
                                           aCCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGa                                               <  1:1760119/101‑1 (MQ=25)
                                            cccGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaa                                              <  1:2894247/101‑1 (MQ=25)
                                             ccGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaaa                                             <  1:2331151/101‑1 (MQ=25)
                                             ccGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaaa                                             <  1:1293813/101‑1 (MQ=25)
                                              cGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAAc                                            <  1:4548538/101‑1 (MQ=25)
                                               gTTCCGGGCAGGCATCAAGGGCCAGCGGGGGGCCGCGTTCTGACTTCAAGGCAG.G...GGGCGC.GGCCCCCGGCCCCCC.GGGAAACTTTCGGCAAGATGAAAcg                                           >  2:3169961/1‑100 (MQ=255)
                                               gTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACa                                           >  2:3831691/1‑101 (MQ=25)
                                                ttCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCCGACCCCC.GGGGTATTTGTGGCAAGATGAAACag                                          >  1:374121/1‑101 (MQ=21)
                                                   cGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGc                                       <  1:940563/101‑1 (MQ=25)
                                                   cGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGc                                       <  2:3785401/101‑1 (MQ=25)
                                                   cGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGc                                       <  1:2011310/101‑1 (MQ=25)
                                                    gggCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCg                                      <  1:1014329/101‑1 (MQ=25)
                                                     ggcaggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGt                                     <  1:1854126/101‑1 (MQ=25)
                                                      gcaggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGtt                                    <  1:3420198/101‑1 (MQ=25)
                                                      gcaggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGtt                                    <  1:489868/101‑1 (MQ=25)
                                                       caggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTa                                   <  2:3640509/101‑1 (MQ=25)
                                                        aggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.g                                 <  1:2977766/101‑1 (MQ=25)
                                                        aggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.g                                 <  2:710388/101‑1 (MQ=25)
                                                         ggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.gg                                <  1:1494121/101‑1 (MQ=25)
                                                          gcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.ggg                               <  2:731328/101‑1 (MQ=25)
                                                            aTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAt                             >  2:2228531/1‑101 (MQ=25)
                                                             tCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAtt                            <  1:577311/101‑1 (MQ=25)
                                                             tCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAtt                            <  1:1812893/101‑1 (MQ=25)
                                                             tCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAtt                            <  2:4272001/101‑1 (MQ=25)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                           <  1:1633858/101‑1 (MQ=25)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                           <  2:3251003/101‑1 (MQ=25)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                           <  1:1188005/101‑1 (MQ=25)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                           <  2:2602739/101‑1 (MQ=25)
                                                               aaGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTg                          >  1:2240275/1‑101 (MQ=25)
                                                               aaGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CC.CG..GGGCGC.TGCCCCCGGGCCCCC.CGGACATTTGTGGCCAGATGAAACAGAGCGTTA.GGGAGTTg                          >  2:1809991/1‑101 (MQ=255)
                                                                aGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTgg                         <  2:259183/101‑1 (MQ=25)
                                                                  ggCCAGCCGGGGGGCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GCGGTAATTGTGGCAAGATGAAACCGAGCGCTA.GGGGATTggga                       >  1:2732542/1‑99 (MQ=16)
                                                                   gCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTaa                      <  2:1280788/101‑1 (MQ=25)
                                                                    ccAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAAc                     <  2:2080013/101‑1 (MQ=25)
                                                                    ccAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAAc                     >  1:2142244/1‑101 (MQ=25)
                                                                      aGCCGGGGGTCGAGTTCTGATCTCAAGG.CC.CG..GGGGGG.CGCCCCCCGGCCCCC.CGGGGATTTTTGGGAAGATTGAAAAAAGCGGTA.GGGGGTTGGTaaacc                   >  2:3900098/1‑98 (MQ=255)
                                                                       gCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCAt                  <  1:1355258/101‑1 (MQ=25)
                                                                        ccGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATg                 <  1:618654/101‑1 (MQ=25)
                                                                        ccGGGGGTCGAGTTCTGATCTCAAGG.CC.CG..GGGGGCGGGCCCCCGGCCCCCCGGGGATTTTTGGGGC.GGA.GAAACCAAGCGGTAGGGGGTTTGGTAAcatg                  >  1:3452271/1‑98 (MQ=255)
                                                                        ccGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGGGGC.GCCCCCCCGCCCCCC.GGGGT.TTTTTGGGAAAATGAAAAAAAACGGTA.GGGATTTGGGAAcacag                 >  2:3416479/1‑97 (MQ=255)
                                                                         cGGGGGTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGa                <  1:2497421/101‑1 (MQ=25)
                                                                           ggggTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATc              <  2:857420/101‑1 (MQ=25)
                                                                           ggggTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATc              <  1:4319288/101‑1 (MQ=25)
                                                                           ggggTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATc              <  2:1357310/101‑1 (MQ=25)
                                                                            gggTCGAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCg             <  2:1979531/101‑1 (MQ=25)
                                                                              gTCGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAAAAGAGCGTTA.GGGATTTGGTAACCATGATTggg           >  1:3120171/1‑101 (MQ=21)
                                                                               tCGAGTTCTGATCTCAAGG.CCGG...GGGGGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGGTTTGGGAACCATGATCGGGc          >  2:535324/1‑101 (MQ=21)
                                                                               tCGAGTTCTGATCTC.AAG.GC.CGG.GGGGGC.TGCCCCCGGGCCCCC.CGGGTATTTGTGGCAAGATGAAAAAGAGCGGTA.GGGATTTGGTAACCACTATCGGGc          >  2:3616099/1‑101 (MQ=16)
                                                                                cGAGTTCTGATCTC.AAG.GC.CGG.GGGGGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAAAGCGTTA.GGGGTTTGGTAACCATGATCGGGCa         >  1:4620925/1‑101 (MQ=21)
                                                                                cGAGTTCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCCGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCa         >  2:2736295/1‑101 (MQ=25)
                                                                                 gAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCaa        <  2:443048/101‑1 (MQ=25)
                                                                                 gAGTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCaa        <  1:1104832/101‑1 (MQ=25)
                                                                                   gTTCTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGGCCCCC.GGGCTATTTTTGGCACGATGAAACAGAGCGTTA.GGGGTTTGGTAACCATGATCGGGCAAGc      >  1:1427004/1‑101 (MQ=25)
                                                                                    ttCTGATCTC.AAG.GC.CGG.GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCt     >  2:588248/1‑101 (MQ=25)
                                                                                      cTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTgg   <  2:4372782/101‑1 (MQ=25)
                                                                                      cTGATCTCAAGG.CCGG...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTgg   <  2:510542/101‑1 (MQ=25)
                                                                                                |                                                                                                  
ATCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAAG.GC.C...GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGC  >  NC_014034/2209579‑2209765

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG