Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,078 C→G 1.9% T193T (ACG→ACC)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0780C→G 1.9% 4.0 ‑1.4 0.0335T193T (ACG→ACC) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (181/145):  total (184/151)
Fisher's exact test strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 596 total aligned reads displayed.

CGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221978‑222178
                                                                                                    |                                                                                                    
cggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                                                                                                      <  2:4119051/101‑1 (MQ=11)
 ggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGAACGGTCGGGTCCGTGGGATCGGTCGGGTCGGTCGGATCGgtcgggtccg                                                                                                     >  2:3159523/1‑101 (MQ=11)
  ggggggtccgtggggtccgtgggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgt                                                                                                    <  1:2509747/98‑1 (MQ=11)
   tcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtg                                                                                                   <  1:1141204/101‑2 (MQ=11)
   tcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtc                                                                                                   <  2:390780/101‑1 (MQ=11)
   gcggggccgtggggtccgtcggATCCGTGGGATCGGGCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtg                                                                                                   <  1:1422133/100‑2 (MQ=11)
   gcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtc                                                                                                   <  1:41764/100‑1 (MQ=11)
    gggctcggtcgggtccgtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                  >  2:1769304‑M1/38‑101 (MQ=255)
    cgggctcgtggggtgcgtcggATCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGgtcggatccgtag                                                                                                  <  2:1580712/95‑3 (MQ=11)
    cggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                  >  1:1999155/1‑101 (MQ=255)
     gggtccgtggggtcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgg                                                                                                 <  1:1202786/101‑1 (MQ=14)
     gggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcgg                                                                                                 >  1:2742957/1‑101 (MQ=11)
      ggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGGGGGATCGGTCGGGGCCGTGGGATCGgtcgggtccgcgggg                                                                                                >  1:3479807/1‑96 (MQ=11)
      ggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCAGTGGGATCGGTCGGGGCCGTGGGGTCGgtcgggtccgtgggg                                                                                                >  2:1368415/1‑101 (MQ=11)
      gatgcgtgggatcggtcggATCGGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                                <  2:679260/97‑2 (MQ=14)
      gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                                >  1:3371206/3‑100 (MQ=255)
      gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                                >  2:2288117/3‑100 (MQ=255)
       atccgtgggatcggtcggGTCCGCGGGATCGGTCGGGTCCGTGGGATCGGTCGGGACCGTGGGAGCGGTCGGGTCCGATGGATCGggcggatccgtcggac                                                                                               >  2:192311/2‑99 (MQ=11)
       atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                               >  2:1334330/2‑99 (MQ=255)
         ccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtgggggcc                                                                                             >  2:4092742/1‑101 (MQ=11)
         ccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTAGGGTCCGTCGGATCCgtggggtccgtcgggccc                                                                                             >  1:4068714/1‑101 (MQ=11)
          cgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGtcgggtccgtggggtccg                                                                                            >  1:1518720/1‑101 (MQ=14)
           gtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGtcggggccgtggggtgcgg                                                                                           >  2:917942/1‑100 (MQ=11)
           gtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgt                                                                                           >  2:2750169/1‑101 (MQ=14)
           gtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggt                                                                                           >  2:4396436/1‑101 (MQ=11)
            tggggtcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                          <  2:3402857/101‑2 (MQ=11)
            tgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATGGGTCGGGTCCGTGGGATCCGTGGGGTCCTTGGGGTCGGTg                                                                                          >  2:4529966/1‑101 (MQ=11)
            tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCGGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                          <  1:2445331/101‑2 (MQ=11)
            ggggatcggtcggTTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                          <  1:2656432/100‑2 (MQ=11)
             gggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtcg                                                                                         <  2:425937/101‑3 (MQ=11)
             cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCTGTCGGGTCCGTGGGATCGGTCGGGTCTGTAGGATCGGTCGCGTCCGTCGGGTCCGGgg                                                                                         >  1:3930411/11‑101 (MQ=11)
              gggtccgtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                        <  2:1501030‑M1/74‑1 (MQ=255)
                gtccgtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAt                                                                                      >  1:496892‑M1/26‑101 (MQ=255)
                 tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                     <  2:3522244/101‑1 (MQ=11)
                 tccgtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCTTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtc                                                                                     <  1:4436443‑M1/77‑1 (MQ=255)
                 ccgtccggATCGGGTGGATCGGCCGGATTCGTGGGGTCGGTCGGATCTGGGGGATAGGTCGGATCTGTGGGATCGGTCGGATCCGTGGGATCGGTCGGAtc                                                                                     <  2:3353066/96‑1 (MQ=9)
                   cgtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGggatggg                                                                                   <  2:1023635‑M1/79‑4 (MQ=255)
                   cgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGTCGGATCGGTGGGAtccg                                                                                   >  1:2368918/2‑99 (MQ=11)
                    gtgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGatcggc                                                                                  <  2:3464772‑M1/80‑2 (MQ=255)
                    gtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGGCCGTCGGCTCCGTGGGGtcggc                                                                                  >  1:3307279/4‑100 (MQ=14)
                    gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggg                                                                                  >  2:4330624/1‑100 (MQ=14)
                     tgggctccgtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                 >  2:626364‑M1/21‑101 (MQ=255)
                     tgggatcggtcggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTGGGAtcggtc                                                                                 <  2:3100572‑M1/81‑1 (MQ=255)
                     tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGCCCGGCGGGGCAGTGGGAtcggtc                                                                                 >  2:2229743/1‑101 (MQ=14)
                     tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                 <  1:4643608/101‑1 (MQ=14)
                     tcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtg                                                                                 >  1:2311802/1‑100 (MQ=11)
                      cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                                <  2:553441/101‑1 (MQ=14)
                      cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCCGTGGGAtcggtcg                                                                                >  2:3994693/1‑101 (MQ=14)
                      cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                                >  1:3641215/1‑99 (MQ=11)
                      cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGGCGGGTCCGTCGGATAGGTCGGAtccgtgg                                                                                >  1:4344723/1‑99 (MQ=11)
                        gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgggg                                                                              >  2:3696882/1‑100 (MQ=11)
                        gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgggc                                                                              >  2:2052796/1‑100 (MQ=11)
                        gATCCGGGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGa                                                                              <  2:2334533/101‑1 (MQ=11)
                          tcggtgggatccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATc                                                                            >  1:3550023‑M1/16‑101 (MQ=255)
                          cccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATc                                                                            <  2:2017560/100‑1 (MQ=11)
                            cggtggctcggtcgcGTCCGTGGGCTCGGTCGGCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCg                                                                          <  2:115280/94‑1 (MQ=14)
                            cGTGGGATCGGGCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                                          <  1:3788163/101‑1 (MQ=14)
                               gggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtcg                                                                       <  1:2160757/101‑3 (MQ=14)
                                   tccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                   <  2:365049‑M1/95‑1 (MQ=255)
                                   gcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCCGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc                                                                   <  1:3141197/100‑1 (MQ=14)
                                    cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCCGTCGGGTCCGTGGGGTCGGTGGGATCGGTGGGGTCCGTGGGAtcg                                                                  >  2:1882480/1‑101 (MQ=14)
                                    cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcg                                                                  <  2:2719412/101‑1 (MQ=14)
                                    ccgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                  >  1:1266462‑M1/6‑101 (MQ=255)
                                     cgtgGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                                                                 <  1:3690575‑M1/97‑1 (MQ=255)
                                      gtcggGTCCGTCGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                                                                <  1:3704164/101‑1 (MQ=14)
                                        cggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtcg                                                              >  1:2859587/1‑101 (MQ=17)
                                        cggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGCCCGTCGGATCCGTGGGGCCCGTCGGGTCCGTGGGATCGGGCGGGTGCGTCGGAgcggtgg                                                              >  2:4612280/1‑99 (MQ=18)
                                         ggatCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGACCGGTCGGGGCCGCAGAAtcggtccg                                                             >  2:485224/4‑99 (MQ=17)
                                          ggTCGGTGGGATCGGTGGGGTCCGTGGGGTCGGTGGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                            <  1:1042452/101‑1 (MQ=18)
                                          gatCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAGCGGCCGGGCCCGCAGGATCGGTCggg                                                            >  2:770615/3‑101 (MQ=14)
                                            tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                          <  2:2905214/101‑1 (MQ=17)
                                            tCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTc                                                          <  1:773331/101‑1 (MQ=255)
                                             ccGTGGGAGCGGTCGGGTCGGTGGGGTCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                         <  2:184926/101‑1 (MQ=18)
                                              cGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGCTGGGCCGGGCCCg                                                        >  1:2216112/1‑101 (MQ=17)
                                              cGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATAGGTAGGATCCGTGGGGTCCGTGGGATCGGACGGGTACGTGGGAtcggtcgcgacag                                                        >  2:2386843/1‑95 (MQ=14)
                                              cGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCg                                                        >  2:3823815/1‑101 (MQ=17)
                                                tcggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTg                                                      >  1:294804/3‑101 (MQ=18)
                                                 cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTAGGTCGGGTCCGTgg                                                     >  2:106404/2‑101 (MQ=17)
                                                     tcggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCt                                                 >  2:4708268/1‑101 (MQ=255)
                                                      cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                                >  2:3322213/1‑101 (MQ=255)
                                                         gcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                             <  1:1863376/100‑1 (MQ=255)
                                                          cggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtgg                                            >  2:3749841/1‑101 (MQ=255)
                                                          cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggttg                                            >  1:2082038/1‑93 (MQ=14)
                                                            ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGa                                          >  1:1000596/3‑101 (MQ=255)
                                                                cgggggATCGGTCGGAGCCGTAGGATCGGTGGGAGCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAGCGGTGGGGTCCGTGGGGCTGGTGGGAtcgg                                      <  1:3053621/98‑1 (MQ=18)
                                                                cGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGggggttgg                                      >  2:2124079/1‑96 (MQ=255)
                                                                  tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGGtcggtc                                    >  1:55383/1‑101 (MQ=255)
                                                                     gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGa                                 >  1:320623/1‑101 (MQ=255)
                                                                             gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                         >  1:1738001/1‑101 (MQ=255)
                                                                                tCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                      >  1:2049397/1‑101 (MQ=255)
                                                                                          cggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAc            <  1:1904082/101‑1 (MQ=255)
                                                                                          cggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAc            >  2:2474479/1‑101 (MQ=255)
                                                                                           ggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAcc           >  1:1620800/1‑100 (MQ=255)
                                                                                           ggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACa           >  2:2630131/1‑101 (MQ=255)
                                                                                           ggtcggGTCCGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACa           <  2:1235896/101‑1 (MQ=255)
                                                                                              ggggtccgtcggatccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGatcggtcgggtccgtggggtcggtcgggtccgtgggatcggtcg        <  2:2562727‑M2/100‑45 (MQ=255)
                                                                                               gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc       <  2:4192721/101‑1 (MQ=255)
                                                                                               gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc       >  1:455634/1‑101 (MQ=255)
                                                                                                 gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa     <  2:2995431/101‑1 (MQ=255)
                                                                                                    cgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggc  >  1:895764/1‑101 (MQ=255)
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CGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221978‑222178

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG