Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,232,209 T→C 100% T767A (ACC→GCC)  RCAP_rcc02068 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,232,2090T→C100.0% 668.0 178T767A (ACC→GCC) RCAP_rcc02068ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (84/93):  ref base (1/0):  total (85/93)

Only 100 of 182 total aligned reads displayed.

ACCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGCC  >  NC_014034/2232111‑2232306
                                                                                                  |                                                                                                 
 ccGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGAt                                                                                                <  2:158969/101‑1 (MQ=255)
 ccGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGAt                                                                                                <  1:177647/101‑1 (MQ=255)
  cGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATc                                                                                               >  2:400006/1‑101 (MQ=255)
   gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                              <  2:3070125/101‑1 (MQ=255)
    aTCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTg                                                                                             <  2:78706/101‑1 (MQ=255)
    aTCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTg                                                                                             >  2:1404472/1‑101 (MQ=255)
    aTCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTg                                                                                             >  2:417615/1‑101 (MQ=255)
    aTCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTg                                                                                             >  1:1961492/1‑101 (MQ=255)
     tCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTgg                                                                                            <  1:2396103/101‑1 (MQ=255)
     tCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTgg                                                                                            >  2:2032545/1‑101 (MQ=255)
         ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                        <  1:3977138/101‑1 (MQ=255)
         ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                        <  2:4134172/101‑1 (MQ=255)
         ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                        <  2:3739197/101‑1 (MQ=255)
          gCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGa                                                                                       >  2:578043/1‑101 (MQ=255)
           ccGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAg                                                                                      <  1:1222257/101‑1 (MQ=255)
           ccGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAg                                                                                      >  1:244281/1‑101 (MQ=255)
           ccGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAg                                                                                      <  2:1879678/101‑1 (MQ=255)
             gTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCt                                                                                    <  2:1834940/101‑1 (MQ=255)
              tCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                   >  2:2306271/1‑101 (MQ=255)
              tCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                   <  1:2355199/101‑1 (MQ=255)
               cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                  <  2:2386392/101‑1 (MQ=255)
               cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                  <  2:3088254/101‑1 (MQ=255)
                gACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAg                                                                                 >  1:4448083/1‑101 (MQ=255)
                  cAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcc                                                                               <  1:1621313/101‑1 (MQ=255)
                    gCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCTCCGTGCACAGCGCGGCAATCTGGGAGGAGCACAGaccc                                                                             >  1:1559631/1‑101 (MQ=37)
                     cGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCt                                                                            >  2:1256641/1‑101 (MQ=255)
                     cGTCGCCACCTGCGTGGCCAACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCt                                                                            <  1:2683811/101‑1 (MQ=255)
                      gTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCtt                                                                           >  2:1466029/1‑101 (MQ=255)
                       tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                          <  1:2102635/101‑1 (MQ=255)
                       ccGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                          <  1:1404566/100‑1 (MQ=255)
                         gCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCTAGCTCAGCCCCTTGAt                                                                        <  2:949060/101‑1 (MQ=255)
                          ccACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATc                                                                       >  1:778551/1‑101 (MQ=255)
                           cACCTTCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCATCCCCTTGATCg                                                                      <  2:2915246/101‑1 (MQ=255)
                            cccTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGc                                                                     <  2:1162100/100‑1 (MQ=255)
                             ccTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCa                                                                    >  1:1452462/1‑101 (MQ=255)
                              cTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAg                                                                   >  1:2714052/1‑101 (MQ=255)
                               tGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGc                                                                  <  1:3409648/101‑1 (MQ=255)
                                 cGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCg                                                                <  2:187364/101‑1 (MQ=255)
                                  gTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGt                                                               >  1:628454/1‑101 (MQ=255)
                                      ccGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCgag                                                           <  2:2283191/101‑1 (MQ=255)
                                       cGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCgaga                                                          <  2:3899761/101‑1 (MQ=255)
                                       cGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCgaga                                                          <  1:1368791/101‑1 (MQ=255)
                                        gACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGagag                                                         >  2:4151287/1‑101 (MQ=255)
                                         aCAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgc                                                        >  2:1514479/1‑101 (MQ=255)
                                          cAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgcg                                                       >  1:3043122/1‑101 (MQ=255)
                                           aGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgcgc                                                      >  1:2986248/1‑101 (MQ=255)
                                            gCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGcgcg                                                     <  2:3363398/101‑1 (MQ=255)
                                             cGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCgcgg                                                    >  1:4674275/1‑101 (MQ=255)
                                             cGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCgcgg                                                    >  1:637768/1‑101 (MQ=255)
                                              gTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCgcggc                                                   <  2:3460174/101‑1 (MQ=255)
                                                  ccgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGaaa                                               <  2:1838134/101‑1 (MQ=255)
                                                   cgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAAt                                              >  2:4612467/1‑101 (MQ=255)
                                                   cgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAAt                                              <  2:4436488/101‑1 (MQ=255)
                                                    gccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATc                                             <  1:1868048/101‑1 (MQ=255)
                                                     ccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATcc                                            >  2:3726435/1‑101 (MQ=255)
                                                     ccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATcc                                            <  2:4273106/101‑1 (MQ=255)
                                                      cTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCTAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccg                                           <  2:3753173/101‑1 (MQ=255)
                                                        gcgcCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccgcc                                         <  1:1641604/101‑1 (MQ=255)
                                                        gcgcCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccgcc                                         <  2:2753430/101‑1 (MQ=255)
                                                         cgcCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccgccg                                        >  2:2673529/1‑101 (MQ=255)
                                                          gcCGTGGTCAGGGCGTCGATCTGCCCCGTGGTCAGCGCGGCAATCTGGGTCGAGCCCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGt                                       <  1:3171144/101‑1 (MQ=255)
                                                            cGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCt                                     <  1:366438/101‑1 (MQ=255)
                                                            cGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCt                                     <  1:368412/101‑1 (MQ=255)
                                                             gTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTg                                    <  2:1986477/101‑1 (MQ=255)
                                                              tGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGc                                   >  1:4561330/1‑101 (MQ=255)
                                                              tGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGc                                   <  1:183729/101‑1 (MQ=255)
                                                                gTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAg                                 <  2:1184724/101‑1 (MQ=255)
                                                                gTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAg                                 >  1:1600966/1‑101 (MQ=255)
                                                                gTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAg                                 <  2:3258092/101‑1 (MQ=255)
                                                                   aGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAgcgc                              >  1:108430/1‑101 (MQ=255)
                                                                    gggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGcgcg                             >  1:1132598/1‑101 (MQ=255)
                                                                    gggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGcgcg                             >  2:321272/1‑101 (MQ=255)
                                                                     ggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCgg                            >  1:2753612/1‑101 (MQ=255)
                                                                      gCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGga                           <  1:202612/101‑1 (MQ=255)
                                                                       cGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgag                          >  2:1016062/1‑101 (MQ=255)
                                                                       cGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgag                          <  2:3586987/101‑1 (MQ=255)
                                                                       cGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgag                          >  2:2524414/1‑101 (MQ=255)
                                                                          cGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATc                       >  2:3143967/1‑101 (MQ=255)
                                                                           gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                      <  1:1028714/101‑1 (MQ=255)
                                                                            aTCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTg                     >  1:863862/1‑101 (MQ=255)
                                                                            aTCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGACCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTg                     >  2:1513359/1‑101 (MQ=255)
                                                                             tCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgc                    <  1:4151533/101‑1 (MQ=255)
                                                                             tCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgc                    <  1:1016141/101‑1 (MQ=255)
                                                                             tCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgc                    <  1:2250962/101‑1 (MQ=255)
                                                                             tCTGCCCCGTGCTCAGCGCGGCGATCTGGGTAGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGAGAAATCCGCCGTCTGCAGCGCGGAGATCTgc                    >  1:4244309/1‑101 (MQ=255)
                                                                               tGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgcgc                  >  2:3429410/1‑101 (MQ=255)
                                                                               tGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgcgc                  >  2:4634250/1‑101 (MQ=255)
                                                                                   ccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTg              <  1:3448386/101‑1 (MQ=255)
                                                                                   ccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTg              <  1:3100425/101‑1 (MQ=255)
                                                                                    cGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGc             >  1:4342454/1‑101 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            <  2:1063971/101‑1 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            >  2:608618/1‑101 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            >  1:4210621/1‑101 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            <  1:1570107/101‑1 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            <  2:4667466/101‑1 (MQ=255)
                                                                                      tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg           <  2:1961375/101‑1 (MQ=255)
                                                                                      tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg           <  1:1514580/101‑1 (MQ=255)
                                                                                       gCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGa          <  1:510391/101‑1 (MQ=255)
                                                                                            gcgcGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgcc     <  2:3235306/101‑1 (MQ=255)
                                                                                               cgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCgccgcc  >  2:3874867/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ACCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGCC  >  NC_014034/2232111‑2232306

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG