Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 5.8% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 5.8% 25.8 ‑1.8 ‑0.0240intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/10):  ref base (111/115):  total (115/125)
Fisher's exact test strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 253 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414
                                                                                                     |                                                                                                             
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                               >  2:382171/1‑101 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                              >  2:3448114/1‑101 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                              >  2:4102467/1‑101 (MQ=255)
   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                            <  2:1181269/101‑1 (MQ=255)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                           >  1:4536770/1‑101 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                         >  1:2500066/1‑101 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGGCT.CCCCCGGGATATTTGAGGCAAGa                                                                                                        <  1:1263339/101‑1 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                        >  2:3652664/1‑101 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                        <  1:4498563/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                      >  1:875348/1‑101 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                      <  1:937087/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                      >  2:3706615/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                     >  1:2929949/1‑101 (MQ=255)
            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                >  2:765073/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               >  1:305220/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGAGCACGAAGGGGGCTCTGCCCCCGCCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                               <  1:4056803/101‑1 (MQ=38)
              agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                              <  1:706662/101‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g..g                                                                                       <  1:4465017/101‑1 (MQ=255)
                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..Gc                                                                                      >  1:1688696/1‑101 (MQ=255)
                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCtt                                                                                    >  2:1077866/1‑101 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCttt                                                                                   <  2:4441165/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTc                                                                                  <  2:3300152/101‑1 (MQ=255)
                        ccgatctaGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCAt                                                                                <  1:3894049/94‑1 (MQ=38)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCAt                                                                                <  1:1839708/101‑1 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCAt                                                                                <  2:1681112/101‑1 (MQ=255)
                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATc                                                                               <  2:4323906/101‑1 (MQ=255)
                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATc                                                                               <  1:3143135/101‑1 (MQ=255)
                           ccGGAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCtt                                                                             <  2:3259628/101‑1 (MQ=255)
                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGc                                                                           >  1:1944058/1‑101 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGcc                                                                          <  2:1224722/101‑1 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCAtagatcgg                                                                         >  2:3893807/1‑94 (MQ=37)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCg                                                                         <  2:2876313/101‑1 (MQ=255)
                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGa                                                                        >  1:2042628/1‑101 (MQ=255)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                       >  2:2955988/1‑101 (MQ=255)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                       >  1:1738481/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAat                                                                    <  1:765147/101‑1 (MQ=255)
                                      gcccccGGGGCAATAAGGGGGCCCTGCCCCCGTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAatat                                                                  <  1:4084169/98‑1 (MQ=37)
                                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAatat                                                                  <  2:808356/101‑1 (MQ=255)
                                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATc                                                                 >  2:2456024/1‑101 (MQ=255)
                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcg                                                                <  1:1446144/101‑2 (MQ=255)
                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcc                                                                >  2:4658457/1‑101 (MQ=255)
                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcc                                                                <  2:447138/101‑1 (MQ=255)
                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATccc                                                               >  1:3872759/1‑101 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                              <  1:2174623/101‑1 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                              <  2:3666721/101‑1 (MQ=255)
                                            ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                            <  2:2131654/101‑1 (MQ=255)
                                            ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                            >  1:324954/1‑101 (MQ=255)
                                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggggg                                                         <  2:219761/101‑1 (MQ=255)
                                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggggg                                                         >  2:2081947/1‑101 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       >  2:4170689/1‑101 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       <  1:4301777/101‑1 (MQ=255)
                                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                     <  1:3706845/101‑1 (MQ=255)
                                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                     <  1:2897477/101‑1 (MQ=255)
                                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                   <  1:2366384/101‑1 (MQ=255)
                                                    aagGGGGCTCGGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                   <  1:3553316/101‑1 (MQ=255)
                                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                  <  1:2222555/101‑1 (MQ=255)
                                                     agGCGGCTCTCCCCCCGCCCCGTCCCGGGCCC.CCCCCGGTATTTTGGAGGCAAGATGAG...GGGG..G..CCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                  <  2:883553/101‑1 (MQ=16)
                                                       ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                >  2:3586307/1‑101 (MQ=255)
                                                       ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                >  2:674612/1‑101 (MQ=255)
                                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                              <  1:110420/101‑1 (MQ=255)
                                                          gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                             <  2:3136736/101‑1 (MQ=255)
                                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                         >  1:264106/6‑101 (MQ=255)
                                                             cttcccccGTCCCGTGCCGGGTCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                        <  1:4425168/97‑1 (MQ=255)
                                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                        <  1:3605719/97‑1 (MQ=255)
                                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGG..G..GCTTTCATCTTGCCG.AAATATCCCCGGGGGGGAA.TGGCGCA..GCCa                                       >  1:3928765/5‑101 (MQ=37)
                                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                      >  2:60642/3‑101 (MQ=255)
                                                               gcccccGTCCCCTGCCGGGGCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                      <  1:2817521/99‑1 (MQ=255)
                                                                 ccgcgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                    >  1:3510041/4‑101 (MQ=255)
                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                 >  2:45967/1‑101 (MQ=255)
                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAgggg                                 >  2:2556298/1‑101 (MQ=255)
                                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                <  2:1738376/101‑1 (MQ=255)
                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              >  2:4294425/1‑101 (MQ=255)
                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              <  2:3924319/101‑1 (MQ=255)
                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              <  2:1634417/101‑1 (MQ=255)
                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             <  2:3214748/101‑1 (MQ=255)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGT..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            <  2:3509829/101‑1 (MQ=255)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            >  2:2785752/1‑101 (MQ=255)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg                            >  1:1718570/1‑101 (MQ=255)
                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                <  2:4734452/94‑1 (MQ=18)
                                                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                               <  2:4360232/95‑1 (MQ=18)
                                                                           tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcc                          >  2:1468707/1‑100 (MQ=255)
                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                        >  1:1325977/1‑101 (MQ=255)
                                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                     >  2:2239127/1‑101 (MQ=255)
                                                                                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg.c                        <  1:527047/101‑1 (MQ=17)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                 >  1:358914/1‑98 (MQ=255)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                 <  1:3089351/101‑4 (MQ=255)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                 <  1:4020275/101‑4 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                <  1:1557905/101‑5 (MQ=255)
                                                                                     gcccccGGGATATTTGGGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                <  1:4347372/100‑5 (MQ=255)
                                                                                      cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcg               >  1:812051/1‑96 (MQ=255)
                                                                                        cccGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgc                 <  2:4544891/101‑3 (MQ=0)
                                                                                         ccGGGATATTTGAGGCAAGATGAA...GGGG..T..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgg             <  2:305186/101‑1 (MQ=255)
                                                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccc               >  2:2731666/1‑97 (MQ=0)
                                                                                          cGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccc               >  2:3553439/1‑97 (MQ=2)
                                                                                          cGGGATATTTGAGGCAAGATGAA...GGGG..G..TCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcggg            <  2:180712/101‑1 (MQ=255)
                                                                                           gggATATTTGGGGCAAGAGGAG...GGGG..G..GCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGGA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc           <  1:988755/101‑1 (MQ=25)
                                                                                            ggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcg          >  1:2113736/1‑101 (MQ=255)
                                                                                                atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGccc      >  1:106802/1‑100 (MQ=255)
                                                                                                 tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAA.TGGCGGA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc     <  1:2774647/101‑1 (MQ=37)
                                                                                                    gAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc  <  1:2674363/101‑1 (MQ=255)
                                                                                                     |                                                                                                             
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG