Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,332 G→T 2.4% intergenic (‑128/‑177) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3320G→T 2.4% 3.7 0.0 ‑0.1213intergenic (‑128/‑177)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (94/112):  total (98/115)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.12e-01

Only 100 of 235 total aligned reads displayed.

ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431
                                                                                                          |                                                                                                      
aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...ggggg                                                                                                        >  2:2498421/1‑101 (MQ=255)
   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                                     >  1:3122530/1‑101 (MQ=255)
   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                                     >  1:3924561/1‑101 (MQ=255)
    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                    >  2:1077866/1‑101 (MQ=255)
    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                    >  1:2131775/1‑101 (MQ=255)
     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                                   <  2:3351928/101‑1 (MQ=255)
      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                                                  <  1:3604567/101‑1 (MQ=255)
           ccGGAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                             <  2:3259628/101‑1 (MQ=255)
           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                             <  1:1730877/101‑1 (MQ=255)
           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                             <  2:3219537/101‑1 (MQ=255)
            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                                            <  1:522966/101‑1 (MQ=255)
              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                                          <  1:344655/101‑1 (MQ=255)
               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCAtagatcgg                                                                                         >  2:3893807/1‑94 (MQ=37)
                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                                        >  2:3153886/1‑101 (MQ=255)
                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                       >  1:4492283/1‑101 (MQ=255)
                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                       >  1:1738481/1‑101 (MQ=255)
                   tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGCCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                                                     >  2:2854077/1‑101 (MQ=255)
                    gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                    <  1:2093568/101‑1 (MQ=255)
                    gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                    <  1:765147/101‑1 (MQ=255)
                     ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                                                   <  1:3749929/101‑1 (MQ=255)
                      gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                                  <  2:808356/101‑1 (MQ=255)
                       cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:2507587/101‑1 (MQ=255)
                        aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcg                                                                                <  1:1446144/101‑2 (MQ=255)
                        aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                >  2:4658457/1‑101 (MQ=255)
                        aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                <  2:447138/101‑1 (MQ=255)
                         cccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                                               >  1:956930/1‑101 (MQ=255)
                          ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                              <  2:2943062/101‑1 (MQ=255)
                          ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                              >  2:1646760/1‑101 (MQ=255)
                            ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                                                            >  1:324954/1‑101 (MQ=255)
                                cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                        >  1:841037/1‑100 (MQ=255)
                                 aagaagGGGGCTCTAC.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                       >  2:4733758/1‑99 (MQ=255)
                                  agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                      >  2:4347125/1‑101 (MQ=255)
                                   gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                     <  1:2897477/101‑1 (MQ=255)
                                    aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                    <  1:2366384/101‑1 (MQ=255)
                                    aagGGGGCTCG.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                    <  1:3553316/101‑1 (MQ=255)
                                     agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                   <  1:2222555/101‑1 (MQ=255)
                                     agGGGCCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGT.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                   <  1:4413950/101‑1 (MQ=38)
                                       ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                 <  1:1297624/101‑1 (MQ=255)
                                       ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                 >  2:3586307/1‑101 (MQ=255)
                                       ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                 >  2:674612/1‑101 (MQ=255)
                                        gggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                >  1:4157999/1‑101 (MQ=255)
                                         ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                                                               <  1:110420/101‑1 (MQ=255)
                                              cttcccccGTCCCGTGCCGGGTCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                           <  1:4425168/97‑1 (MQ=255)
                                                gcccccGTCCCCTGCCGGGGCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                         <  1:2817521/99‑1 (MQ=255)
                                                 cccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                                                        >  2:4300532/2‑101 (MQ=255)
                                                   cccgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                                                      >  1:2206632/1‑101 (MQ=255)
                                                    ccgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAATGGCGCAGCCAGAggg                                                     <  2:1978608/101‑1 (MQ=255)
                                                      gtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                                                   <  2:1738376/101‑1 (MQ=255)
                                                         ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                >  2:4528299/1‑101 (MQ=255)
                                                         ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                >  2:2747181/1‑101 (MQ=255)
                                                         ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                >  2:2215383/1‑101 (MQ=255)
                                                          cgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGTGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                                               <  2:3509829/101‑1 (MQ=255)
                                                          cgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                                               >  1:296729/1‑101 (MQ=255)
                                                           gtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAg.c                                             >  2:4617883/1‑101 (MQ=255)
                                                           gcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAgggggg                                                  <  1:3576625/95‑1 (MQ=18)
                                                            tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cg                                            <  2:4149620/101‑1 (MQ=255)
                                                            tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cg                                            >  2:82292/1‑101 (MQ=255)
                                                               gcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAg.c                                             <  1:991578/99‑1 (MQ=18)
                                                               ccattcaccccccGGG.ATATTTTCGGCAAGATGAA...AGCCCCCCTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCATAGGGGGGCAG.CG.cc                                         >  2:3809676/9‑101 (MQ=11)
                                                                  gACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccc                                    >  1:4029250/1‑100 (MQ=255)
                                                                  gACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccc                                    <  1:737818/101‑2 (MQ=255)
                                                                   gcT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                   <  2:245395/100‑3 (MQ=255)
                                                                   aCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                   <  2:3656213/101‑3 (MQ=255)
                                                                   aCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                   >  2:4380080/1‑99 (MQ=255)
                                                                    cT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccctt                                  <  1:3089351/101‑4 (MQ=255)
                                                                    cT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccctt                                  <  1:3889935/101‑4 (MQ=255)
                                                                     t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                 <  2:1608705/101‑5 (MQ=255)
                                                                     t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                 <  1:1557905/101‑5 (MQ=255)
                                                                     t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                 <  2:3059914/101‑5 (MQ=255)
                                                                     cccccccGGGAATTTTGGAGGAAAGAGGAG...GGGGGGTTTTCTTTTTGCAAAAATTACCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                   <  1:3981906/99‑3 (MQ=12)
                                                                      gcccccGGG.ATATTTGGGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                 <  1:4347372/100‑5 (MQ=255)
                                                                       cccccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttcg                                <  1:4528552/101‑6 (MQ=255)
                                                                         cccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTcgcg                               <  2:1213634/101‑1 (MQ=255)
                                                                         cccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTcgcg                               >  2:1272730/1‑101 (MQ=255)
                                                                          ccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgg                              <  2:607886/101‑1 (MQ=255)
                                                                            ggg.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggc                            >  2:487361/1‑101 (MQ=255)
                                                                             gg.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggcg                           <  1:2643380/101‑1 (MQ=255)
                                                                              g.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggcgg                          >  2:3999898/1‑101 (MQ=255)
                                                                                atatTTGAGGCAAGATGAA...GGGGGGCTTTCACCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGggcggc                         >  1:4206156/1‑101 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAG...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGcc                        <  2:3019066/101‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGcc                        >  2:2176177/1‑101 (MQ=255)
                                                                                  atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGccc                       >  1:106802/1‑100 (MQ=255)
                                                                                   tttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAATGGCGGAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCcgc                      <  1:2774647/101‑1 (MQ=37)
                                                                                    ttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCcgcg                     <  2:264081/101‑1 (MQ=255)
                                                                                     tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGa                    >  1:170387/1‑101 (MQ=255)
                                                                                     tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGa                    >  1:1903821/1‑101 (MQ=255)
                                                                                      gAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAc                   <  1:1953156/101‑1 (MQ=255)
                                                                                       gggaaaGATGAG...GGGGGGTTTTCATCTTCCCGAAAATACCGGGGGGGGGGAAGGGCGCAGCCAGGGGGGGGCAGCCC.CCC.CCTTCGCGGGCGGCCGCGAcg                  <  1:3329894/97‑1 (MQ=255)
                                                                                         gCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAcgcg                >  1:1395202/1‑101 (MQ=255)
                                                                                           aaGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAcgcgcg              >  2:1824702/1‑101 (MQ=255)
                                                                                           aaGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAcgcgcg              >  2:3307546/1‑101 (MQ=255)
                                                                                            aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc             >  2:3871958/1‑101 (MQ=255)
                                                                                             gATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc            <  2:2847709/101‑1 (MQ=255)
                                                                                              aTGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgccg           <  2:1350217/101‑1 (MQ=255)
                                                                                               tGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGccccac          >  2:3969880/1‑99 (MQ=255)
                                                                                                  a...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc       >  1:2704383/1‑101 (MQ=255)
                                                                                                      ggggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa      >  1:2461999/1‑101 (MQ=255)
                                                                                                         gggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCCGCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt   >  2:243587/1‑101 (MQ=255)
                                                                                                          ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc  <  2:4536517/101‑1 (MQ=255)
                                                                                                          ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc  <  1:3663229/101‑1 (MQ=255)
                                                                                                          |                                                                                                      
ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG