Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,333 G→C 4.2% intergenic (‑129/‑176) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3330G→C 4.2% 10.3 ‑3.2 ‑0.0213intergenic (‑129/‑176)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (1/8):  ref base (97/106):  total (98/115)
Fisher's exact test strand distribution p-value = 3.98e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.74e-01

Only 100 of 234 total aligned reads displayed.

TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432
                                                                                                          |                                                                                                      
  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                                      >  1:3122530/1‑101 (MQ=255)
   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                     >  2:1077867/1‑101 (MQ=255)
   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                     >  1:2131775/1‑101 (MQ=255)
    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                                    <  2:3351928/101‑1 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                                                   <  1:3604567/101‑1 (MQ=255)
       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                                                 <  2:1681112/101‑1 (MQ=255)
        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                                                                >  1:3635466/1‑101 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                                               <  2:2447652/101‑1 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                                               <  2:3535093/101‑1 (MQ=255)
          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                              <  2:3219537/101‑1 (MQ=255)
          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                              <  1:1730877/101‑1 (MQ=255)
             cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                                           <  1:344655/101‑1 (MQ=255)
              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCAtagatcgg                                                                                          >  2:3893807/1‑94 (MQ=37)
              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                                          <  2:2876313/101‑1 (MQ=255)
               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                                         >  2:3153886/1‑101 (MQ=255)
                cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                        >  1:1738481/1‑101 (MQ=255)
                cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                        >  1:4492283/1‑101 (MQ=255)
                   gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                     <  1:765147/101‑1 (MQ=255)
                   gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                     <  1:2093568/101‑1 (MQ=255)
                    ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                                                    <  1:3749929/101‑1 (MQ=255)
                     gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                                   <  2:808356/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                                  <  1:2507587/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                                  >  2:2456024/1‑101 (MQ=255)
                       aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcg                                                                                 <  1:1446144/101‑2 (MQ=255)
                       aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                 <  2:447138/101‑1 (MQ=255)
                       aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                 >  2:4658457/1‑101 (MQ=255)
                        cccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                                                >  1:956930/1‑101 (MQ=255)
                         ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                               <  2:2943062/101‑1 (MQ=255)
                           ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                                                             <  2:2131654/101‑1 (MQ=255)
                            gggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                                                            >  2:3269850/1‑101 (MQ=255)
                              gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                          <  2:219761/101‑1 (MQ=255)
                              gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                          >  2:3089151/1‑101 (MQ=255)
                                aagaagGGGGCTCTAC.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                        >  2:4733758/1‑99 (MQ=255)
                                aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                        <  1:4301777/101‑1 (MQ=255)
                                 agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                       >  2:4347125/1‑101 (MQ=255)
                                  gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                      <  1:2897477/101‑1 (MQ=255)
                                   aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                     <  1:2366384/101‑1 (MQ=255)
                                    agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                    <  1:2222555/101‑1 (MQ=255)
                                    agGGGCCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGT.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                    <  1:4413950/101‑1 (MQ=38)
                                      ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  <  1:1297624/101‑1 (MQ=255)
                                      ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  >  2:3586307/1‑101 (MQ=255)
                                      ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  >  2:674612/1‑101 (MQ=255)
                                       gggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                 >  1:4157999/1‑101 (MQ=255)
                                        ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                                                                <  1:110420/101‑1 (MQ=255)
                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                                                             >  1:999976/6‑101 (MQ=255)
                                               gcccccGTCCCCTGCCGGGGCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                          <  1:2817521/99‑1 (MQ=255)
                                                cccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                                                         >  2:4300532/2‑101 (MQ=255)
                                                  cccgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                                                       >  1:2206632/1‑101 (MQ=255)
                                                   ccgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAATGGCGCAGCCAGAggg                                                      <  2:1978608/101‑1 (MQ=255)
                                                    cgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                                                     >  1:4450149/1‑101 (MQ=255)
                                                        ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                 >  2:4528299/1‑101 (MQ=255)
                                                        ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                 >  2:2747181/1‑101 (MQ=255)
                                                         cgcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                                    <  1:3922950/94‑1 (MQ=18)
                                                          gtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAg.c                                              >  2:4617883/1‑101 (MQ=255)
                                                          gcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAgggggg                                                   <  1:3576625/95‑1 (MQ=18)
                                                           tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cg                                             <  2:4149620/101‑1 (MQ=255)
                                                           tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cg                                             >  2:82292/1‑101 (MQ=255)
                                                           tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cc                                             >  2:1468707/1‑100 (MQ=255)
                                                             ccGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cc                                          >  1:3550859/1‑101 (MQ=255)
                                                              ccattcaccccccGGG.ATATTTTCGGCAAGATGAA...AGCCCCCCTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCATAGGGGGGCAG.CG.cc                                          >  2:3809676/9‑101 (MQ=11)
                                                                ggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAg.cg.c                                           <  1:527047/101‑1 (MQ=17)
                                                                 gACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccc                                     <  1:737818/101‑2 (MQ=255)
                                                                 gACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccc                                     >  1:4029250/1‑100 (MQ=255)
                                                                  aCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                    <  2:3656213/101‑3 (MQ=255)
                                                                  aCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                    >  2:4380080/1‑99 (MQ=255)
                                                                   cT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccctt                                   <  1:3089351/101‑4 (MQ=255)
                                                                   cT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccctt                                   <  1:3889935/101‑4 (MQ=255)
                                                                    t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                  <  2:1608705/101‑5 (MQ=255)
                                                                    t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                  <  1:1557905/101‑5 (MQ=255)
                                                                    cccccccGGGAATTTTGGAGGAAAGAGGAG...GGGGGGTTTTCTTTTTGCAAAAATTACCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.ccct                                    <  1:3981906/99‑3 (MQ=12)
                                                                     gcccccGGG.ATATTTGGGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttc                                  <  1:4347372/100‑5 (MQ=255)
                                                                      cccccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc.cccttcg                                 <  1:4528552/101‑6 (MQ=255)
                                                                        cccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTcgcg                                <  2:1213634/101‑1 (MQ=255)
                                                                         ccGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgg                               <  2:607886/101‑1 (MQ=255)
                                                                          cGGG.ATATTTGAGGCAAGATGAA...GGGGGTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcggg                              <  2:180712/101‑1 (MQ=255)
                                                                          cGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcggg                              >  1:851451/1‑101 (MQ=255)
                                                                           ggg.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggc                             >  2:487361/1‑101 (MQ=255)
                                                                            gg.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggcg                            <  1:2643380/101‑1 (MQ=255)
                                                                             g.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCgcgggcgg                           >  2:3999898/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAG...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGcc                         <  2:3019066/101‑1 (MQ=255)
                                                                                tatTTGAGGCAAGAGGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGcc                         <  1:3167913/101‑1 (MQ=255)
                                                                                 atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGccc                        >  1:106802/1‑100 (MQ=255)
                                                                                 atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCcg                        >  2:2656498/1‑101 (MQ=255)
                                                                                    tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGa                     >  1:170387/1‑101 (MQ=255)
                                                                                    tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGa                     >  1:1903821/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAc                    <  1:1953156/101‑1 (MQ=255)
                                                                                      gggaaaGATGAG...GGGGGGTTTTCATCTTCCCGAAAATACCGGGGGGGGGGAAGGGCGCAGCCAGGGGGGGGCAGCCC.CCC.CCTTCGCGGGCGGCCGCGAcg                   <  1:3329894/97‑1 (MQ=255)
                                                                                        gCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAcgcg                 >  1:1395202/1‑101 (MQ=255)
                                                                                          aaGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGAcgcgcg               >  2:3307546/1‑101 (MQ=255)
                                                                                           aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc              >  2:3871958/1‑101 (MQ=255)
                                                                                           aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc              >  2:1297249/1‑101 (MQ=255)
                                                                                            gATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc             <  2:2847709/101‑1 (MQ=255)
                                                                                             aTGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGGGGCCGCGACGCGggccg            >  1:2914682/1‑101 (MQ=25)
                                                                                              tGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGccccac           >  2:3969880/1‑99 (MQ=255)
                                                                                                 a...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc        >  1:2704383/1‑101 (MQ=255)
                                                                                                     ggggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa       <  1:2747345/101‑1 (MQ=255)
                                                                                                     ggggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa       >  1:2461999/1‑101 (MQ=255)
                                                                                                        gggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCCGCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt    >  2:243587/1‑101 (MQ=255)
                                                                                                         ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc   <  2:4536517/101‑1 (MQ=255)
                                                                                                          gCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAG.GGCCCC.CCTTCCCGGGCGGCCGCGCGGCGCGCCGCacaaggccg  >  2:1752640/1‑93 (MQ=25)
                                                                                                          |                                                                                                      
TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG