Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,334 C→A 2.3% intergenic (‑130/‑175) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3340C→A 2.3% 2.1 ‑0.4 ‑0.2219intergenic (‑130/‑175)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (99/115):  total (102/117)
Fisher's exact test strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.67e-01

Only 100 of 234 total aligned reads displayed.

GCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGT  >  NC_014034/2249235‑2249433
                                                                                                         |                                                                                                     
 cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                                      >  1:4324153/1‑101 (MQ=255)
  acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                     >  2:1077867/1‑101 (MQ=255)
  acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                                                     >  1:2447788/1‑101 (MQ=255)
   caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                                    <  2:3351928/101‑1 (MQ=255)
    aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                                                   >  1:3060102/1‑101 (MQ=255)
      ccgatctaGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                                                 <  1:3894049/94‑1 (MQ=38)
      aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                                                 <  2:1681112/101‑1 (MQ=255)
       tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                                                                <  1:3143135/101‑1 (MQ=255)
        aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                                               <  2:2447652/101‑1 (MQ=255)
        aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                                               <  2:3535093/101‑1 (MQ=255)
         ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                              <  2:3219537/101‑1 (MQ=255)
         ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                                                              <  1:1730877/101‑1 (MQ=255)
             aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAtagatcgg                                                                                          >  2:3893807/1‑94 (MQ=37)
             aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                                          <  2:2876313/101‑1 (MQ=255)
              gCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                                         >  2:3153886/1‑101 (MQ=255)
               cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                        >  1:4492283/1‑101 (MQ=255)
               cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                                        >  1:1738481/1‑101 (MQ=255)
                  gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                     <  1:765147/101‑1 (MQ=255)
                  gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                                     <  1:2898986/101‑1 (MQ=255)
                    gcccccGGGGCAATAAGGGGGCCCT.GCCCCCGTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                                   <  1:4084169/98‑1 (MQ=37)
                    gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                                   <  2:808356/101‑1 (MQ=255)
                    gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                                                   <  1:2824843/101‑1 (MQ=255)
                     cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                                  >  2:2456024/1‑101 (MQ=255)
                      aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcg                                                                                 <  1:1446144/101‑2 (MQ=255)
                      aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                 >  2:4658457/1‑101 (MQ=255)
                      aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                                                 <  2:447138/101‑1 (MQ=255)
                       cccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                                                >  1:956930/1‑101 (MQ=255)
                       cccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                                                                >  1:3872759/1‑101 (MQ=255)
                        ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                               <  2:2943062/101‑1 (MQ=255)
                        ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                               <  1:2174623/101‑1 (MQ=255)
                          ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                                                             <  2:2131654/101‑1 (MQ=255)
                           gggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                                                            >  2:3269850/1‑101 (MQ=255)
                             gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                          <  2:219761/101‑1 (MQ=255)
                             gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                          >  2:3089151/1‑101 (MQ=255)
                               aagaagGGGGCTCTAC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                        >  2:4733758/1‑99 (MQ=255)
                                agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                       >  2:4347125/1‑101 (MQ=255)
                                 gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                      <  1:3559251/101‑1 (MQ=255)
                                 gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                      <  1:3706845/101‑1 (MQ=255)
                                     ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  >  2:3586307/1‑101 (MQ=255)
                                     ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  <  1:1297624/101‑1 (MQ=255)
                                     ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                  >  2:674612/1‑101 (MQ=255)
                                       ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                                                                <  1:110420/101‑1 (MQ=255)
                                           tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                                                             >  1:264106/6‑101 (MQ=255)
                                           tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                                                             >  1:999976/6‑101 (MQ=255)
                                            cttcccccGTCCCGTGCCGGGTCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                            <  1:4425168/97‑1 (MQ=255)
                                            ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                            <  1:3605719/97‑1 (MQ=255)
                                            ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGGGGCTTTCATCTTGCCG.AAATATCCCCGGGGGGGAATGGCGCAGCCa                                                           >  1:3928765/5‑101 (MQ=37)
                                               cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                                                         >  2:4300532/2‑101 (MQ=255)
                                                ccgcgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                                                        >  1:3510041/4‑101 (MQ=255)
                                                ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                                                        <  1:3116734/101‑1 (MQ=255)
                                                  ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAATGGCGCAGCCAGAggg                                                      <  2:1978608/101‑1 (MQ=255)
                                                       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                 >  2:4528299/1‑101 (MQ=255)
                                                       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                                 >  2:2747181/1‑101 (MQ=255)
                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAgc                                               >  2:4617883/1‑101 (MQ=255)
                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                                              <  2:4149620/101‑1 (MQ=255)
                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                                              >  2:82292/1‑101 (MQ=255)
                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcc                                              >  2:1468707/1‑100 (MQ=255)
                                                           gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg.c                                            >  1:233057/1‑101 (MQ=255)
                                                             ccattcaccccccGGGATATTTTCGGCAAGATGAA...AGCCCCCCTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCATAGGGGGGCAGCG.cc                                           >  2:3809676/9‑101 (MQ=11)
                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAgcg.c                                            <  1:2744523/101‑1 (MQ=17)
                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAgcg.c                                            <  1:527047/101‑1 (MQ=17)
                                                                gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccc                                      <  1:737818/101‑2 (MQ=255)
                                                                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccct                                     >  2:4380080/1‑99 (MQ=255)
                                                                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccct                                     <  2:3656213/101‑3 (MQ=255)
                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGTGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccctt                                    <  1:2472012/101‑4 (MQ=255)
                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccctt                                    >  1:358914/1‑98 (MQ=255)
                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.ccctt                                    <  1:4020275/101‑4 (MQ=255)
                                                                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.cccttc                                   <  1:1557905/101‑5 (MQ=255)
                                                                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.cccttc                                   <  2:1608705/101‑5 (MQ=255)
                                                                     cccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccc.cccttcg                                  <  1:4528552/101‑6 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTcgcg                                 <  2:1213634/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcgg                                <  2:607886/101‑1 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAA...GGGGGTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcggg                               <  2:180712/101‑1 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcggg                               >  1:851451/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcgggc                              >  2:487361/1‑101 (MQ=255)
                                                                           ggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcgggcg                             >  1:2113736/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCgcgggcgg                            >  2:3999898/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATGAA...GGGGGGCTTTCACCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGggcggc                           >  1:4206156/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATGAG...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGcc                          <  2:3019066/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGccc                         >  1:106802/1‑100 (MQ=255)
                                                                               atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCcg                         >  2:2656498/1‑101 (MQ=255)
                                                                                tttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCcgc                        >  1:277983/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGa                      >  1:3362466/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGa                      >  1:170387/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGa                      >  1:1903821/1‑101 (MQ=255)
                                                                                   gAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAc                     <  1:2674363/101‑1 (MQ=255)
                                                                                   gAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAc                     <  1:1953156/101‑1 (MQ=255)
                                                                                      gCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgcg                  >  1:1395202/1‑101 (MQ=255)
                                                                                        aaGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgcgcg                >  2:3307546/1‑101 (MQ=255)
                                                                                         aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc               >  1:3305551/1‑101 (MQ=255)
                                                                                         aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc               >  2:1297249/1‑101 (MQ=255)
                                                                                         aGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc               >  2:3871958/1‑101 (MQ=255)
                                                                                          gATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc              <  2:2847709/101‑1 (MQ=255)
                                                                                          gATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc              <  1:4351254/101‑1 (MQ=255)
                                                                                            tGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGccccac            >  2:3969880/1‑99 (MQ=255)
                                                                                               a...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGCCGCGCGCCGCAAc         >  1:2214648/1‑101 (MQ=255)
                                                                                                      gggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCGCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt     >  2:243587/1‑101 (MQ=255)
                                                                                                       ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc    <  2:4536517/101‑1 (MQ=255)
                                                                                                        gCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAGGGCCCC.CCTTCCCGGGCGGCCGCGCGGCGCGCCGCacaaggccg   >  2:1752640/1‑93 (MQ=25)
                                                                                                         cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt  >  1:2047668/1‑101 (MQ=255)
                                                                                                         |                                                                                                     
GCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGT  >  NC_014034/2249235‑2249433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG