Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 6.4% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 6.4% 24.3 ‑3.0 ‑0.3202intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/10):  ref base (99/90):  total (102/100)
Fisher's exact test strand distribution p-value = 4.80e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.37e-01

Only 100 of 223 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                           |                                                                                                           
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                             <  2:2876313/101‑1 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                                            >  2:3153886/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                           <  1:2093815/101‑1 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGCCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                         >  2:2854077/1‑101 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                        <  1:1139155/101‑1 (MQ=255)
       gcccccGGGGCAATAAGGGGGCCCTGCCCCCGTGCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                      <  1:4084169/98‑1 (MQ=37)
       gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                      <  1:2824843/101‑1 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                     <  1:2507587/101‑1 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                     >  2:2456024/1‑101 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                   >  1:3872759/1‑101 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                  <  1:2174623/101‑1 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                  >  2:1646760/1‑101 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                  <  2:2943062/101‑1 (MQ=255)
             ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                >  1:324954/1‑101 (MQ=255)
                gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                             >  2:2081947/1‑101 (MQ=255)
                gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                             >  2:3089151/1‑101 (MQ=255)
                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                           <  2:1187929/101‑1 (MQ=255)
                  aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                           >  2:4170689/1‑101 (MQ=255)
                     aagGGGGCTCGGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                       <  1:3553316/101‑1 (MQ=255)
                      agGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGT.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                      <  1:4413950/101‑1 (MQ=38)
                      agGCGGCTCTCCCCCCGCCCCGTCCCGGGCCC.CCCCCGGT.ATTTTGGAGGCAAGATGAG...GGGG..GCCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                      <  2:883553/101‑1 (MQ=16)
                        ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                    >  2:3586307/1‑101 (MQ=255)
                        ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                    >  2:674612/1‑101 (MQ=255)
                          ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                  <  1:110420/101‑1 (MQ=255)
                             tctgcccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                             >  1:264106/6‑101 (MQ=255)
                             tctgcccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                             >  1:999976/6‑101 (MQ=255)
                              ctgcccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                            <  1:3605719/97‑1 (MQ=255)
                                gcccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                          >  2:60642/3‑101 (MQ=255)
                                 cccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                         >  2:4300532/2‑101 (MQ=255)
                                  ccgcgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                        >  1:3510041/4‑101 (MQ=255)
                                  ccccGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                        <  1:3116734/101‑1 (MQ=255)
                                     cgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  1:4450149/1‑101 (MQ=255)
                                     cgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  2:81113/1‑101 (MQ=255)
                                     cgtcccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  2:45967/1‑101 (MQ=255)
                                         ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                 >  2:2215383/1‑101 (MQ=255)
                                         ccgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                 >  2:4528299/1‑101 (MQ=255)
                                          cgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGT..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                <  2:3509829/101‑1 (MQ=255)
                                          cgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                <  1:4495603/101‑1 (MQ=255)
                                          cgtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                >  1:1718570/1‑101 (MQ=255)
                                          cgcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                    <  1:3922950/94‑1 (MQ=18)
                                          cgcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                    <  2:4734452/94‑1 (MQ=18)
                                           gtGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                              >  2:4617883/1‑101 (MQ=255)
                                           gcaagcggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                   <  1:3576625/95‑1 (MQ=18)
                                            tGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                             >  2:717203/1‑101 (MQ=255)
                                             gCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                           >  1:1325977/1‑101 (MQ=255)
                                             gCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                           >  1:233057/1‑101 (MQ=255)
                                               ccattcaccccccGGG.ATATTTTCGGCAAGATGAA...AGCC..CCCCTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCATAGGGGGGCAG.CG.cc                                                          >  2:3809676/9‑101 (MQ=11)
                                                 ggACTCCCCCCGGG.ATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg.c                                                           <  1:527047/101‑1 (MQ=17)
                                                   actccccccGGG.ATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.CG.ccc                                                         <  2:412404/97‑1 (MQ=17)
                                                   aCT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccct                                                     >  2:4380080/1‑99 (MQ=255)
                                                     t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                   <  1:1557905/101‑5 (MQ=255)
                                                     t.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                   <  2:1608705/101‑5 (MQ=255)
                                                     cccccccGGGAATTTTGGAGGAAAGAGGAG...GGGG..GGTTTTCTTTTTGCAAAAATTACCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccct                                                     <  1:3981906/99‑3 (MQ=12)
                                                      gcccccGGG.ATATTTGGGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                   <  1:4347372/100‑5 (MQ=255)
                                                       cccccGTC.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                                                  <  1:756246/101‑6 (MQ=255)
                                                       cccccGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttcg                                                  >  1:812051/1‑96 (MQ=255)
                                                         cccGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTcgcg                                               >  2:1272730/1‑101 (MQ=255)
                                                          ccGGG.ATATTTGAGGCAAGATGAA...GGGG..TGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCgcgg                                              <  2:305186/101‑1 (MQ=255)
                                                           cGGG.ATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCC..tgccc                                                >  2:2731666/1‑97 (MQ=0)
                                                           cGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCgcggg                                             >  1:851451/1‑101 (MQ=255)
                                                            ggg.ATATTTGGGGCAAGAGGAG...GGGG..GGCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGGA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCgcgggc                                            <  1:988755/101‑1 (MQ=25)
                                                            ggg.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCgcgggc                                            >  2:487361/1‑101 (MQ=255)
                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGcc                                        >  2:2176177/1‑101 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGccc                                       >  1:106802/1‑100 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCcg                                       <  2:324920/101‑1 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCcg                                       >  2:1946907/1‑101 (MQ=255)
                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCcgc                                      >  1:277983/1‑101 (MQ=255)
                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCcgcg                                     <  2:264081/101‑1 (MQ=255)
                                                                      gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGAc                                   <  1:1953156/101‑1 (MQ=255)
                                                                       gggaaaGATGAG...GGGG..GGTTTTCATCTTCCCGAAAATACCGGGGGGGGGGAA.GGGCGCA..GCCAGGGGGGGGCAGCCC.CCCCC..TTCGCGGGCGGCCGCGAcg                                  <  1:3329894/97‑1 (MQ=255)
                                                                         gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGAcgcg                                >  1:1395202/1‑101 (MQ=255)
                                                                          aaaGATGAG...GGGG..GGTTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGAcgcgc                               <  1:4168614/100‑1 (MQ=25)
                                                                            aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACgcgcg.c                            >  2:3871958/1‑101 (MQ=255)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGcg.cc                           <  2:2847709/101‑1 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGGGGCCGCGACGCGgg.ccg                          >  1:2914682/1‑101 (MQ=25)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGcg.ccg                          <  2:1350217/101‑1 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGcg.ccg                          >  2:3922272/1‑101 (MQ=255)
                                                                               tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGcc.ccac                         >  2:3969880/1‑99 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAAc                      <  2:334307/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGCCGCGCG.CCGCAAc                      >  1:2214648/1‑101 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAAc                      >  1:2704383/1‑101 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAAc                      <  2:244620/101‑1 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACa                     <  1:2747345/101‑1 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACa                     >  1:2461999/1‑101 (MQ=255)
                                                                                            ggCTTTGATCTTGCCGAAACTAGCCCGGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCGG.CGCCCCCC..TTCGCGGGCGGCCGCGAGGCGCG.CCGCAACAGGTc                 <  2:4687873/101‑1 (MQ=25)
                                                                                            ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTc                 <  2:4536517/101‑1 (MQ=255)
                                                                                             gCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.GGCCCCCC..TTCCCGGGCGGCCGCGCGGCGCG.CCGCacaaggccg                >  2:1752640/1‑93 (MQ=25)
                                                                                              cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGt               >  1:2047668/1‑101 (MQ=255)
                                                                                               tttCTTCTGGCGAAAAATCCCCGGGGGGGTGAA.TGGCGCC..GCCAGGGGGGGGCCG.CC.CCCCCCTTTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGt               <  2:4029008/101‑1 (MQ=255)
                                                                                                ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGc             >  2:1795035/1‑101 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCGG.CC.CCCCC..CTCGCGGGGCGCCCCGACGCGGGCCCGAAACAGGCGGTGcc            >  2:2356170/1‑101 (MQ=16)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGccc           <  2:358880/101‑1 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGcccc          >  2:3437060/1‑101 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCtt        >  1:1769054/1‑101 (MQ=255)
                                                                                                        gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGt     <  1:4658713/101‑1 (MQ=255)
                                                                                                        gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGt     <  1:674676/101‑1 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCa   >  1:3733537/1‑101 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.CCCCC..TTCGCGGGGGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCAt  <  1:1468801/101‑1 (MQ=38)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCAt  <  1:4294672/101‑1 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCAt  <  1:3002827/101‑1 (MQ=255)
                                                                                                           |                                                                                                           
AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGG.ATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCC..TTCGCGGGCGGCCGCGACGCGCG.CCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG