Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,348 A→G 6.5% intergenic (‑144/‑161) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3480A→G 6.5% 25.4 ‑2.5 ‑1.1201intergenic (‑144/‑161)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/10):  ref base (94/93):  total (98/103)
Fisher's exact test strand distribution p-value = 8.37e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.24e-02

Only 100 of 227 total aligned reads displayed.

GCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATT  >  NC_014034/2249249‑2249447
                                                                                                            |                                                                                                          
 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                                           >  2:2955988/1‑101 (MQ=255)
 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                                           <  1:2093815/101‑1 (MQ=255)
   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaaaa                                                                                                         >  2:2854077/1‑101 (MQ=255)
    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAat                                                                                                        <  1:1139155/101‑1 (MQ=255)
    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAat                                                                                                        <  1:765147/101‑1 (MQ=255)
      gcccccGGGGCAATAAGGGGGCCCTGCCCCCGTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAatat                                                                                                      <  1:4084169/98‑1 (MQ=37)
       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATc                                                                                                     >  2:2456024/1‑101 (MQ=255)
        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcc                                                                                                    >  2:4658457/1‑101 (MQ=255)
        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATcc                                                                                                    <  2:447138/101‑1 (MQ=255)
         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATccc                                                                                                   >  1:3872759/1‑101 (MQ=255)
          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                                                                  <  2:3666721/101‑1 (MQ=255)
          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                                                                  >  2:1646760/1‑101 (MQ=255)
            ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                                                <  1:909655/101‑1 (MQ=255)
             gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggg                                                                                               >  2:3269850/1‑101 (MQ=255)
                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggggggg                                                                                            >  1:841037/1‑100 (MQ=255)
                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                            <  1:4423044/101‑1 (MQ=255)
                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                           <  2:1187929/101‑1 (MQ=255)
                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGAGATTTGAGGCAAGATAAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                           >  1:4688129/1‑101 (MQ=38)
                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                         <  1:3559251/101‑1 (MQ=255)
                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                         <  1:2897477/101‑1 (MQ=255)
                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                      <  1:2222555/101‑1 (MQ=255)
                     agGCGGCTCTCCCCCCGCCCCGTCCCGGGCCC.CCCCCGGTATTTTGGAGGCAAGATGAG...GGGG..G..CCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                      <  2:883553/101‑1 (MQ=16)
                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                  <  1:110420/101‑1 (MQ=255)
                          gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                 <  2:3136736/101‑1 (MQ=255)
                           ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                              <  2:3472424/101‑1 (MQ=255)
                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                             >  1:999976/6‑101 (MQ=255)
                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..Gc                                                                             >  1:14326/6‑101 (MQ=255)
                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                          >  2:60642/3‑101 (MQ=255)
                               gcccccGTCCCCTGCCGGGGCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                          <  1:2817521/99‑1 (MQ=255)
                                cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                         >  2:4300532/2‑101 (MQ=255)
                                  cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                       >  1:2206632/1‑101 (MQ=255)
                                   ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                      <  2:1978608/101‑1 (MQ=255)
                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  2:45967/1‑101 (MQ=255)
                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  2:81113/1‑101 (MQ=255)
                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                     >  1:4450149/1‑101 (MQ=255)
                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                    <  2:1738376/101‑1 (MQ=255)
                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                  <  2:3880444/101‑1 (MQ=255)
                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                 >  2:2215383/1‑101 (MQ=255)
                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                 <  1:1781634/101‑1 (MQ=255)
                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                 <  2:3214748/101‑1 (MQ=255)
                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                >  1:1718570/1‑101 (MQ=255)
                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                    <  1:3922950/94‑1 (MQ=18)
                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                    <  2:4734452/94‑1 (MQ=18)
                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                              >  2:4617883/1‑101 (MQ=255)
                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                   <  2:4360232/95‑1 (MQ=18)
                                           tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                             <  2:4149620/101‑1 (MQ=255)
                                           tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                             >  2:717203/1‑101 (MQ=255)
                                           tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cc                                                             >  2:1468707/1‑100 (MQ=255)
                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                           >  1:1325977/1‑101 (MQ=255)
                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                           >  1:233057/1‑101 (MQ=255)
                                             ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c                                                         >  1:3550859/1‑101 (MQ=255)
                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGT..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.cccctt                                                  <  1:2472012/101‑4 (MQ=255)
                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.cccctt                                                  <  1:3089351/101‑4 (MQ=255)
                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.cccctt                                                  >  1:358914/1‑98 (MQ=255)
                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.cccctt                                                  <  1:4020275/101‑4 (MQ=255)
                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.ccccttc                                                 <  2:3059914/101‑5 (MQ=255)
                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.ccccttc                                                 <  1:1557905/101‑5 (MQ=255)
                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.ccccttc                                                 <  2:1608705/101‑5 (MQ=255)
                                                      cccccGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.C.C.CCCt                                                    <  2:3851935/101‑1 (MQ=0)
                                                      cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.ccccttcg                                                <  1:4528552/101‑6 (MQ=255)
                                                        cccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTcgcg                                               >  2:1272730/1‑101 (MQ=255)
                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.C.C.CCCtgccc                                                >  2:2731666/1‑97 (MQ=0)
                                                          cGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.C.C.CCCtgccc                                                >  2:3553439/1‑97 (MQ=2)
                                                           gggATATTTGGGGCAAGAGGAG...GGGG..G..GCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGGA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCgcgggc                                            <  1:988755/101‑1 (MQ=25)
                                                           gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCgcgggc                                            >  2:487361/1‑101 (MQ=255)
                                                            ggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCgcgggcg                                           <  1:2643380/101‑1 (MQ=255)
                                                             gATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCgcgggcgg                                          >  2:3999898/1‑101 (MQ=255)
                                                               tatTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGcc                                        >  2:2176177/1‑101 (MQ=255)
                                                               tatTTGAGGCAAGAGGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGcc                                        <  1:3167913/101‑1 (MQ=255)
                                                                atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGccc                                       >  1:106802/1‑100 (MQ=255)
                                                                 tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAA.TGGCGGA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCcgc                                      <  1:2774647/101‑1 (MQ=37)
                                                                  ttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCcgcg                                     <  2:264081/101‑1 (MQ=255)
                                                                    gAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGAc                                   <  1:1953156/101‑1 (MQ=255)
                                                                        aaaGATGAG...GGGG..G..GTTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGAcgcgc                               <  1:4168614/100‑1 (MQ=25)
                                                                         aaGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGAcgcgcg                              >  1:2517704/1‑101 (MQ=255)
                                                                         aaGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGAcgcgcg                              >  2:1824702/1‑101 (MQ=255)
                                                                          aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACgcgcgc                             >  1:3305551/1‑101 (MQ=255)
                                                                           gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGcgcc                            <  1:4351254/101‑1 (MQ=255)
                                                                           gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGcgcc                            <  2:2847709/101‑1 (MQ=255)
                                                                            aTGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGGGGCCGCGACGCGggccg                           >  1:2914682/1‑101 (MQ=25)
                                                                             tGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGccccac                          >  2:3969880/1‑99 (MQ=255)
                                                                                a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                       <  2:244620/101‑1 (MQ=255)
                                                                                a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                       >  1:2704383/1‑101 (MQ=255)
                                                                                     ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                     >  2:658265/1‑101 (MQ=255)
                                                                                          g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                  <  1:3663229/101‑1 (MQ=255)
                                                                                          g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                  <  2:4536517/101‑1 (MQ=255)
                                                                                              cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                >  1:2047668/1‑101 (MQ=255)
                                                                                                ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc              >  2:1795035/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc            <  2:358880/101‑1 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc           >  2:4121141/1‑101 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt         >  2:3402257/1‑101 (MQ=255)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCAAGAGGGGGGAAG.AG.CAA.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt        >  1:607164/1‑101 (MQ=38)
                                                                                                        gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt      <  1:674676/101‑1 (MQ=255)
                                                                                                        gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt      >  2:230904/1‑101 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa    >  1:3733537/1‑101 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.C.C.CCCTTCGCGGGGGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt   <  1:1468801/101‑1 (MQ=38)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt   <  1:4294672/101‑1 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt   <  1:3448326/101‑1 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt   >  2:2895895/1‑101 (MQ=255)
                                                                                                            aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.CACCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt  >  2:1904205/1‑101 (MQ=255)
                                                                                                            |                                                                                                          
GCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.C.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATT  >  NC_014034/2249249‑2249447

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG