Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,359 G→A 2.5% intergenic (‑155/‑150) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3590G→A 2.5% 4.9 0.0 ‑0.3200intergenic (‑155/‑150)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (99/96):  total (102/98)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.87e-01

Only 100 of 226 total aligned reads displayed.

GGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249261‑2249458
                                                                                                         |                                                                                                          
ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                           <  2:2131654/101‑1 (MQ=255)
   gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                        <  2:219761/101‑1 (MQ=255)
   gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                        >  2:3089151/1‑101 (MQ=255)
     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                      <  2:1187929/101‑1 (MQ=255)
     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                      >  2:4170689/1‑101 (MQ=255)
       gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                    <  1:2897477/101‑1 (MQ=255)
       gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                    <  1:3706845/101‑1 (MQ=255)
        aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaag                                                                                                   >  1:1350309/1‑100 (MQ=255)
        aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                                  <  1:2366384/101‑1 (MQ=255)
         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  1:2222555/101‑1 (MQ=255)
         agGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGTATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  1:4413950/101‑1 (MQ=38)
           ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                               <  1:1297624/101‑1 (MQ=255)
             ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                             <  1:110420/101‑1 (MQ=255)
              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                            <  2:3136736/101‑1 (MQ=255)
                tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                                        >  1:264106/6‑101 (MQ=255)
                 cttcccccGTCCCGTGCCGGGTCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                       <  1:4425168/97‑1 (MQ=255)
                 ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                       <  1:3605719/97‑1 (MQ=255)
                 ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGG..GGCTTTCATCTTGCCG.AAATATCCCCGGGGGGGAA.TGGCGCA..GCCa                                                                                      >  1:3928765/5‑101 (MQ=37)
                   gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                     >  2:60642/3‑101 (MQ=255)
                    cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                    <  2:1794228/100‑1 (MQ=255)
                    cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                    >  2:4300532/2‑101 (MQ=255)
                     ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATCTCGCGGGGTGTGAA.TGGCGCA..GCCagag                                                                                   <  2:3586635/101‑1 (MQ=255)
                      cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                  >  1:2206632/1‑101 (MQ=255)
                       ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                 <  2:1978608/101‑1 (MQ=255)
                         gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                               <  2:1738376/101‑1 (MQ=255)
                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                             <  2:3880444/101‑1 (MQ=255)
                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                             <  2:1634417/101‑1 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGT..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           <  2:3509829/101‑1 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           <  1:4495603/101‑1 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  2:2785752/1‑101 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  1:296729/1‑101 (MQ=255)
                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  1:1718570/1‑101 (MQ=255)
                             cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                               <  2:4734452/94‑1 (MQ=18)
                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                              <  1:3576625/95‑1 (MQ=18)
                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                              <  2:4360232/95‑1 (MQ=18)
                               tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                                        >  2:82292/1‑101 (MQ=255)
                               tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                                        >  2:717203/1‑101 (MQ=255)
                                 ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                                                     >  1:3550859/1‑101 (MQ=255)
                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg.c                                                                      <  1:2744523/101‑1 (MQ=17)
                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg.c                                                                      <  1:527047/101‑1 (MQ=17)
                                      gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.ccct                                                              <  2:245395/100‑3 (MQ=255)
                                      actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.CG.cc.c                                                                   <  2:412404/97‑1 (MQ=17)
                                      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.ccct                                                              <  2:3656213/101‑3 (MQ=255)
                                       cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGT..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.ccctt                                                             <  1:2472012/101‑4 (MQ=255)
                                       cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.ccctt                                                             <  1:3089351/101‑4 (MQ=255)
                                       cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.ccctt                                                             <  1:4020275/101‑4 (MQ=255)
                                        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.cccttc                                                            <  1:1557905/101‑5 (MQ=255)
                                          cccccGTCATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.c.cccttcg                                                           <  1:756246/101‑6 (MQ=255)
                                          cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.C.CCt                                                               <  2:3851935/101‑1 (MQ=0)
                                           ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.C.CCtg                                                              >  2:4647477/1‑100 (MQ=0)
                                            cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.C.CCtgc                                                             <  2:4544891/101‑3 (MQ=0)
                                            cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTcgcg                                                          >  2:1272730/1‑101 (MQ=255)
                                             ccGGGATATTTGAGGCAAGATGAA...GGGG..TGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcgg                                                         <  2:305186/101‑1 (MQ=255)
                                              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.C.CCtgccc                                                           >  2:2731666/1‑97 (MQ=0)
                                              cGGGATATTTGAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcggg                                                        <  2:180712/101‑1 (MQ=255)
                                              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcggg                                                        >  1:851451/1‑101 (MQ=255)
                                               gggATATTTGGGGCAAGAGGAG...GGGG..GGCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGGA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcgggc                                                       <  1:988755/101‑1 (MQ=25)
                                               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcgggc                                                       >  2:487361/1‑101 (MQ=255)
                                                ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcgggcg                                                      >  1:2113736/1‑101 (MQ=255)
                                                 gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCgcgggcgg                                                     >  2:3999898/1‑101 (MQ=255)
                                                  atatTTGAGGCAAGATGAA...GGGG..GGCTTTCACCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGggcggc                                                    >  1:4206156/1‑101 (MQ=255)
                                                   tatTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGcc                                                   <  1:3167913/101‑1 (MQ=255)
                                                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGccc                                                  >  1:106802/1‑100 (MQ=255)
                                                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCcg                                                  <  2:324920/101‑1 (MQ=255)
                                                     tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAA.TGGCGGA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCcgc                                                 <  1:2774647/101‑1 (MQ=37)
                                                        gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGAc                                              <  1:1953156/101‑1 (MQ=255)
                                                        gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGAc                                              <  1:2674363/101‑1 (MQ=255)
                                                         gggaaaGATGAG...GGGG..GGTTTTCATCTTCCCGAAAATACCGGGGGGGGGGAA.GGGCGCA..GCCAGGGGGGGGCAGCCC.CC.C.CCTTCGCGGGCGGCCGCGAcg                                             <  1:3329894/97‑1 (MQ=255)
                                                               gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGcgcc                                       <  2:2847709/101‑1 (MQ=255)
                                                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGcgccg                                      <  2:1350217/101‑1 (MQ=255)
                                                                 tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGccccac                                     >  2:3969880/1‑99 (MQ=255)
                                                                    a...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                  <  1:637770/101‑1 (MQ=255)
                                                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                  <  2:334307/101‑1 (MQ=255)
                                                                    a...GGGG..GGCTTTCATCTCGCAGAAAATAGCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                  <  1:230433/101‑1 (MQ=37)
                                                                         ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                >  2:658265/1‑101 (MQ=255)
                                                                          gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                               >  2:4531718/1‑101 (MQ=255)
                                                                           g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CC.CGCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                              >  2:243587/1‑101 (MQ=255)
                                                                                    cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                       >  2:2856412/1‑101 (MQ=255)
                                                                                     aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                      >  2:3437060/1‑101 (MQ=255)
                                                                                       cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                    >  1:1769054/1‑101 (MQ=255)
                                                                                          gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                 >  2:230904/1‑101 (MQ=255)
                                                                                            cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa               >  2:4600832/1‑101 (MQ=255)
                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.CC.C.CCTTCGCGGGGGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:1468801/101‑1 (MQ=38)
                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:4294672/101‑1 (MQ=255)
                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:3448326/101‑1 (MQ=255)
                                                                                              aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt             <  1:3643399/101‑1 (MQ=255)
                                                                                              aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt             >  1:9936/1‑101 (MQ=255)
                                                                                              aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCAC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt             >  2:1904205/1‑101 (MQ=255)
                                                                                                 atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg          >  1:2056425/1‑101 (MQ=255)
                                                                                                 atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg          >  1:4587163/1‑101 (MQ=255)
                                                                                                    tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCGTTGTCATTCTGttg       >  2:2566277/1‑99 (MQ=255)
                                                                                                     cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCCACCGGTCGTGCCCCGTTGTCATTCTGTCGc      >  2:4243977/1‑101 (MQ=38)
                                                                                                     cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc      >  1:4316619/1‑101 (MQ=255)
                                                                                                     cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGCCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc      >  1:4228912/1‑101 (MQ=255)
                                                                                                      ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGCCATTCTGTCGCa     >  1:3894795/1‑101 (MQ=255)
                                                                                                       cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc    >  1:708522/1‑101 (MQ=255)
                                                                                                        ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc   >  2:1074043/1‑101 (MQ=255)
                                                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  2:4075529/1‑101 (MQ=255)
                                                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  1:4103246/1‑101 (MQ=255)
                                                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  2:916540/1‑101 (MQ=255)
                                                                                                         |                                                                                                          
GGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CC.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249261‑2249458

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG