Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,580 G→T 100% T57K (ACG→AAG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5800G→T100.0% 837.8 233T57K (ACG→AAG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (137/96):  ref base (0/0):  total (137/96)

Only 100 of 263 total aligned reads displayed.

CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262482‑2262677
                                                                                                  |                                                                                                 
cTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTc                                                                                                 <  2:4079883/101‑1 (MQ=255)
 ccGCCATTGGGCTTGAACCAATGGCGCCACACGGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                                <  1:2908816/100‑1 (MQ=255)
  cGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAg                                                                                               <  2:761476/101‑1 (MQ=255)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             <  1:4632780/101‑2 (MQ=255)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgcc                                                                                             >  1:2359556/1‑100 (MQ=255)
      aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTTTCTTCCTCAAACTGCTTCAgcccc                                                                                           >  2:2661212/1‑98 (MQ=255)
         gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        <  2:4027992/101‑1 (MQ=255)
            cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                     >  2:4741495/1‑101 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    >  1:469267/1‑101 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    >  2:4097767/1‑101 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    >  1:2874666/1‑101 (MQ=255)
              tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                   >  1:3894455/1‑101 (MQ=255)
              tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                   >  1:3441085/1‑101 (MQ=255)
                 aCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCgag                                                                                <  2:2114230/101‑3 (MQ=255)
                 aCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGa                                                                                <  1:3290860/101‑1 (MQ=255)
                  ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                               <  2:1146058/101‑1 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                              <  2:3498360/101‑1 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                              <  1:1185684/101‑1 (MQ=255)
                    aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                             <  2:865954/101‑1 (MQ=255)
                    aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                             <  2:2068360/101‑1 (MQ=255)
                    aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                             >  1:509799/1‑101 (MQ=255)
                     aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                            >  1:3685382/1‑101 (MQ=255)
                      ttgcgcCCCCCTGGCTCGATCCAGCATCCCATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                           <  1:1561293/99‑1 (MQ=18)
                      tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                           <  1:3963801/101‑1 (MQ=255)
                        gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:1287533/101‑1 (MQ=255)
                        gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:1058948/101‑1 (MQ=255)
                         cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                        >  1:2412959/1‑101 (MQ=255)
                           ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                      <  1:981811/101‑1 (MQ=255)
                             acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    >  2:3970993/1‑101 (MQ=255)
                              cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                   >  2:239601/1‑100 (MQ=255)
                               acTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccgc                                                                  >  2:1996037/1‑99 (MQ=255)
                                  ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCCGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                               <  1:2564770/101‑1 (MQ=18)
                                  ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                               >  2:3014595/1‑101 (MQ=255)
                                   gCTCGATCCAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                              <  1:194404/101‑1 (MQ=18)
                                    cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                             <  2:3481372/101‑1 (MQ=255)
                                    cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                             <  1:2840120/101‑1 (MQ=255)
                                     tCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                            <  1:4596611/101‑1 (MQ=255)
                                      cGATCCAGCATCACATCCGCCACCAGGGGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAGTCCATCCCGCCATACtt                                                           >  2:3860268/1‑101 (MQ=17)
                                       gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          >  2:4631626/1‑101 (MQ=255)
                                        aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATCCtagc                                                         >  1:1856024/1‑98 (MQ=18)
                                          ccAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                       >  1:1605776/1‑101 (MQ=255)
                                          ccAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                       >  2:1713580/1‑101 (MQ=255)
                                          ccAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                       <  2:4446729/101‑1 (MQ=18)
                                           cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                      <  2:1369391/101‑1 (MQ=255)
                                           cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                      >  1:1465148/1‑101 (MQ=255)
                                            aGCATCACCTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCtt                                                     <  2:4366904/101‑1 (MQ=18)
                                             gCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtag                                                    >  2:2416696/1‑99 (MQ=21)
                                             gCATCACATCCGCCACCAGGCGCTTCAGCTAGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAACTCCATCCCGCCATACTTGGCCTTc                                                    >  2:2730061/1‑101 (MQ=18)
                                              cATCCCATCCCCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTGGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCa                                                   <  2:2917648/101‑1 (MQ=18)
                                              cATCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCa                                                   <  2:496495/101‑1 (MQ=18)
                                               aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                  >  1:2926557/1‑101 (MQ=255)
                                               aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                  >  2:3218792/1‑101 (MQ=255)
                                                 cacaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGTATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCt                                                >  2:4595468/1‑101 (MQ=21)
                                                   caTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                              >  1:3156464/1‑101 (MQ=255)
                                                    ctCCGCCTCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                             <  1:3014783/100‑1 (MQ=18)
                                                    aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                             >  2:314799/1‑101 (MQ=255)
                                                     cccGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                            <  1:1002060/100‑1 (MQ=18)
                                                      ccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                           >  2:1776850/1‑101 (MQ=255)
                                                      ccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                           >  1:4431543/1‑101 (MQ=255)
                                                       cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                          >  2:1533818/1‑101 (MQ=255)
                                                       cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                          >  1:2004452/1‑101 (MQ=255)
                                                        gCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                         >  1:1292573/1‑101 (MQ=255)
                                                         ccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                        >  1:1125512/1‑101 (MQ=255)
                                                          caccgGGCGCTTCAGCTTGGCTTTCTCTTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       <  1:970192/101‑1 (MQ=18)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       >  1:420236/1‑101 (MQ=255)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                       >  2:1203287/1‑101 (MQ=255)
                                                           accaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                      >  1:3284257/1‑101 (MQ=255)
                                                             caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                    >  1:3592176/1‑101 (MQ=255)
                                                             caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGATTGTAGAAGGTcg                                    >  1:4084710/1‑101 (MQ=21)
                                                               ggCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgcg                                  >  1:3804652/1‑101 (MQ=255)
                                                                 cccTTCAGCTTGGCGTTCTCTTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                <  1:578085/99‑1 (MQ=21)
                                                                  gtttCAGCTTCGCGTTCTCTTCTTCAAACTGCTTCAGCCGCTTGTCATCGGAAAAATCCATCCCGCCATTCTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                               <  1:205207/99‑1 (MQ=17)
                                                                    ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                             >  2:1926235/1‑101 (MQ=255)
                                                                    ttCAGCTGGGCTTTCTTTTCCTCAAACTGCTTCAGCCGCTTGCCATCGGACAAACCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                             <  1:4741751/101‑1 (MQ=21)
                                                                     tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                            >  2:4711394/1‑101 (MQ=255)
                                                                      tggtttGGCTTCCTCTTCCGCAAACTGCTTCCGCCGCTTGGCATCGGACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                           <  1:3340395/97‑1 (MQ=21)
                                                                       aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAg                          >  2:2527939/1‑101 (MQ=255)
                                                                       aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAg                          >  1:719783/1‑101 (MQ=255)
                                                                        gCTTGGCTTTCTCTTCCTCAAACTGCTCCAGCCGTTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                         <  2:1688680/101‑1 (MQ=21)
                                                                         cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                        >  1:849150/1‑101 (MQ=255)
                                                                           tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                      >  2:90253/1‑101 (MQ=255)
                                                                            gtctttCTCTTCCTAAAACTGCTTCAGCCGCTGGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                     <  2:4528610/97‑1 (MQ=25)
                                                                            ggCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                     <  2:3440878/101‑1 (MQ=255)
                                                                             gCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                    <  1:3032794/101‑1 (MQ=255)
                                                                              cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                   <  1:3718407/101‑1 (MQ=255)
                                                                              cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                   >  1:4091521/1‑101 (MQ=37)
                                                                                ttCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                 <  2:3636253/101‑1 (MQ=25)
                                                                                tcttctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                 <  1:808286/98‑1 (MQ=25)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  1:4364134/1‑101 (MQ=255)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  2:2351313/1‑101 (MQ=37)
                                                                                  ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc               >  1:1022033/1‑101 (MQ=37)
                                                                                    ctTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg             <  2:4200215/101‑1 (MQ=25)
                                                                                      tCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcagatc           >  2:2852524/1‑96 (MQ=25)
                                                                                        cTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa         <  2:558779/101‑1 (MQ=255)
                                                                                         tCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAg        >  1:227022/1‑101 (MQ=255)
                                                                                          cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc       >  1:1818423/1‑101 (MQ=255)
                                                                                           aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCNATGCTTCCGGCAAAGCt      >  2:1423134/1‑101 (MQ=255)
                                                                                            aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc     >  2:4296547/1‑101 (MQ=255)
                                                                                              cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg   >  2:1082967/1‑101 (MQ=255)
                                                                                               tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa  >  1:2659436/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262482‑2262677

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG