Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,586 T→C 100% Q55R (CAG→CGG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5860T→C100.0% 841.5 236Q55R (CAG→CGG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (143/93):  ref base (0/0):  total (143/93)

Only 100 of 268 total aligned reads displayed.

ATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262488‑2262683
                                                                                                  |                                                                                                 
   gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                              <  1:2328481/101‑1 (MQ=255)
     gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                            <  1:3137068/101‑1 (MQ=255)
      cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                           >  2:4741495/1‑101 (MQ=255)
       ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                          >  1:2874666/1‑101 (MQ=255)
       ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                          <  1:4486239/101‑1 (MQ=255)
        tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                         >  1:3894455/1‑101 (MQ=255)
         gAACCAATGGCGCCACACTGGCTCGATCCAGCATCACCTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAtag                                                                                        >  1:2911149/1‑99 (MQ=255)
           aCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGa                                                                                      <  1:3290860/101‑1 (MQ=255)
             cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                                    <  1:1185684/101‑1 (MQ=255)
             cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                                    <  1:131861/101‑1 (MQ=255)
             cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                                    <  2:3498360/101‑1 (MQ=255)
              aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                                   <  2:2068360/101‑1 (MQ=255)
              aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                                   <  2:865954/101‑1 (MQ=255)
               aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                                  >  1:3685382/1‑101 (MQ=255)
                ttgcgcCCCCCTGGCTCGATCCAGCATCCCATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                                 <  1:1561293/99‑1 (MQ=18)
                tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                                 >  1:1134530/1‑101 (MQ=255)
                 ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                                <  1:1608071/101‑1 (MQ=255)
                  gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                               <  1:1058948/101‑1 (MQ=255)
                  gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                               >  1:1772626/1‑101 (MQ=255)
                  gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                               <  1:1287533/101‑1 (MQ=255)
                   cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                              <  1:3740216/101‑1 (MQ=255)
                   cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                              <  2:2834232/101‑1 (MQ=255)
                   cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                              >  1:2412959/1‑101 (MQ=255)
                   cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                              >  1:558830/1‑101 (MQ=255)
                   cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                              <  1:950100/101‑1 (MQ=255)
                     ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                            <  2:3883882/101‑1 (MQ=255)
                     ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                            <  1:981811/101‑1 (MQ=255)
                      cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACCGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                           >  1:4085704/1‑101 (MQ=255)
                         acTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccgc                                                                        >  2:1996037/1‑99 (MQ=255)
                           tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCCTCGGACAAATTCATTccgcca                                                                      >  2:1751374/1‑101 (MQ=18)
                           tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                      <  2:2384/101‑1 (MQ=255)
                           tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                      >  2:2303581/1‑101 (MQ=255)
                            ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCCGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                                     <  1:2564770/101‑1 (MQ=18)
                            ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                                     >  2:3014595/1‑101 (MQ=255)
                            ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACGGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                                     >  2:1114344/1‑101 (MQ=18)
                             gCTCGATCCAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                                    <  1:194404/101‑1 (MQ=18)
                             gCTCGACCCAGCATCCCCCCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                                    <  2:4089561/101‑1 (MQ=18)
                              cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                                   <  1:2840120/101‑1 (MQ=255)
                              cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                                   <  2:3481372/101‑1 (MQ=255)
                                  aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATCCtagc                                                               >  1:1856024/1‑98 (MQ=18)
                                  aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                               >  1:3027459/1‑101 (MQ=255)
                                    ccAGCATCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                             <  2:319123/101‑1 (MQ=18)
                                     cAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCt                                                            >  1:1465148/1‑101 (MQ=255)
                                       gCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtag                                                          >  2:2416696/1‑99 (MQ=21)
                                       gCATCACATCCGCCACCAGGCGCTTCAGCTAGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAACTCCATCCCGCCATACTTGGCCTTc                                                          >  2:2730061/1‑101 (MQ=18)
                                        cATCCCATCCCCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTGGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCa                                                         <  2:2917648/101‑1 (MQ=18)
                                         aTCCCACCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                        <  1:2962846/101‑1 (MQ=18)
                                           cacaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGTATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCt                                                      >  2:4595468/1‑101 (MQ=21)
                                               tCCGCCACCAGGCGCTTCAGCTTGGCGTTCGCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                                  >  1:4221877/1‑101 (MQ=18)
                                               cccGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                                  <  1:1002060/100‑1 (MQ=18)
                                                 cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCAGCCATACTTGGCCTTCAGCTTGTAGa                                                >  1:3990542/1‑101 (MQ=21)
                                                  gCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                               >  2:1465731/1‑101 (MQ=255)
                                                    caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                             >  2:1203287/1‑101 (MQ=255)
                                                       caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                          >  1:3592176/1‑101 (MQ=255)
                                                       caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                          >  2:4390076/1‑101 (MQ=255)
                                                         ggCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgcg                                        >  1:3804652/1‑101 (MQ=255)
                                                           cgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                      >  1:3321030/1‑101 (MQ=255)
                                                           cccTTCAGCTTGGCGTTCTCTTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                      <  1:578085/99‑1 (MQ=21)
                                                            gtttCAGCTTCGCGTTCTCTTCTTCAAACTGCTTCAGCCGCTTGTCATCGGAAAAATCCATCCCGCCATTCTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                                     <  1:205207/99‑1 (MQ=17)
                                                            gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                                     >  2:3718177/1‑101 (MQ=255)
                                                             cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                                    >  2:4633580/1‑101 (MQ=255)
                                                              ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  2:1926235/1‑101 (MQ=255)
                                                              ttCAGCTGGGCTTTCTTTTCCTCAAACTGCTTCAGCCGCTTGCCATCGGACAAACCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   <  1:4741751/101‑1 (MQ=21)
                                                                cAGCTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                                 <  1:3282927/101‑1 (MQ=21)
                                                                  gCTTGGCTTTCTCTTCCTCAAACTGCTCCAGCCGTTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                               <  2:1688680/101‑1 (MQ=21)
                                                                   cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                              >  1:849150/1‑101 (MQ=255)
                                                                    ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                             >  2:3114807/1‑101 (MQ=255)
                                                                     tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                            >  2:90253/1‑101 (MQ=255)
                                                                      gtctttCTCTTCCTAAAACTGCTTCAGCCGCTGGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  2:4528610/97‑1 (MQ=25)
                                                                      ggCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  2:3440878/101‑1 (MQ=255)
                                                                      ggCGTTCCCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  2:2460403/101‑1 (MQ=25)
                                                                        cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                         >  1:4638384/1‑101 (MQ=255)
                                                                          ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       >  2:434689/1‑101 (MQ=255)
                                                                          ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       >  1:1992088/1‑101 (MQ=255)
                                                                          tcttctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       <  1:808286/98‑1 (MQ=25)
                                                                           tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                      >  1:4364134/1‑101 (MQ=255)
                                                                            ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc                     >  1:1022033/1‑101 (MQ=37)
                                                                             tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCGCATGCTTCCg                    >  2:4060989/1‑101 (MQ=25)
                                                                              ctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg                   >  2:4170305/1‑101 (MQ=37)
                                                                                tCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCa                 >  2:1378034/1‑101 (MQ=255)
                                                                                tCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCa                 <  1:4601402/101‑1 (MQ=25)
                                                                                  cTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa               <  2:558779/101‑1 (MQ=255)
                                                                                    cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             >  2:3611452/1‑101 (MQ=255)
                                                                                      aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc           >  2:4296547/1‑101 (MQ=255)
                                                                                      aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTATAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc           >  2:4719763/1‑101 (MQ=21)
                                                                                        cggcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg         <  1:712874/96‑1 (MQ=255)
                                                                                        cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg         >  2:1082967/1‑101 (MQ=255)
                                                                                         tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa        >  1:2659436/1‑101 (MQ=255)
                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  2:398245/4‑101 (MQ=255)
                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  1:4136563/4‑101 (MQ=255)
                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  2:261141/4‑101 (MQ=255)
                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  1:3238351/4‑101 (MQ=255)
                                                                                          gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       >  2:1801779/4‑101 (MQ=255)
                                                                                            ttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt     >  1:347959/2‑101 (MQ=255)
                                                                                            ttCAGCCGCTGGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt     <  2:1245068/100‑1 (MQ=255)
                                                                                             tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    >  1:3368428/1‑101 (MQ=255)
                                                                                             tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    >  2:2231547/1‑101 (MQ=255)
                                                                                             tCAGCCGCTTGGCAGCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    <  2:719710/101‑1 (MQ=25)
                                                                                             ccAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    <  1:2189797/100‑1 (MQ=255)
                                                                                               aGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCgg  >  2:50404/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262488‑2262683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG