Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,738 T→G 100% R4S (AGA→AGC RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,7380T→G100.0% 983.9 258R4S (AGA→AGCRCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (132/126):  ref base (0/0):  total (132/126)

Only 100 of 263 total aligned reads displayed.

GTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGTCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCAAAATCT  >  NC_014034/2262640‑2262835
                                                                                                  |                                                                                                 
 tCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCtt                                                                                                >  2:1255423/1‑101 (MQ=255)
 gcgcgGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCtt                                                                                                <  2:4312244/100‑1 (MQ=255)
   gcgGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCtttt                                                                                              <  2:3132120/101‑1 (MQ=255)
     ggggCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTcc                                                                                            >  2:106788/1‑101 (MQ=255)
     ggggCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTcc                                                                                            >  1:2688730/1‑101 (MQ=255)
      gggCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCt                                                                                           <  1:1220261/101‑1 (MQ=255)
          tGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCAtt                                                                                       >  1:2233606/1‑101 (MQ=255)
           gAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCAttt                                                                                      <  1:478043/101‑1 (MQ=255)
              cccATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGt                                                                                   >  1:1617886/1‑101 (MQ=255)
              cccATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGt                                                                                   <  1:398289/101‑1 (MQ=255)
               ccATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTc                                                                                  >  2:2782431/1‑101 (MQ=255)
                cATGTTTCTGGGAATGCGCGGAAGTCGGCAAACTGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCt                                                                                 <  1:4097230/101‑1 (MQ=25)
                cATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCt                                                                                 >  1:3072362/1‑101 (MQ=255)
                 aTGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTg                                                                                <  2:3980423/101‑1 (MQ=255)
                 aTGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTg                                                                                >  2:3812751/1‑101 (MQ=255)
                    cTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTc                                                                             <  2:3675207/101‑1 (MQ=255)
                    cTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTc                                                                             <  1:2034188/101‑1 (MQ=255)
                     ttCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTcc                                                                            >  1:3457864/1‑101 (MQ=255)
                     ttCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTcc                                                                            <  2:3320828/101‑1 (MQ=255)
                      tCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCt                                                                           <  2:3591957/101‑1 (MQ=255)
                      tCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCt                                                                           <  1:1244293/101‑1 (MQ=255)
                       ccGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCtt                                                                          >  1:3761429/1‑101 (MQ=255)
                        cGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTtc                                                                         >  2:4173401/1‑101 (MQ=255)
                        cGGCAAAGCTCGGAAGTCGGCAAACAGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTtc                                                                         <  1:1011307/101‑1 (MQ=255)
                         ggCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTtct                                                                        >  2:3736919/1‑101 (MQ=255)
                           cAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCa                                                                      <  2:4221630/101‑1 (MQ=255)
                            aaaGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAg                                                                     <  2:849076/101‑1 (MQ=255)
                            aaaGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAg                                                                     >  1:252841/1‑101 (MQ=255)
                             aaGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAgg                                                                    >  2:4662196/1‑101 (MQ=255)
                               gCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGtt                                                                  >  2:2466324/1‑101 (MQ=255)
                               gCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGtt                                                                  <  1:1465825/101‑1 (MQ=255)
                                cTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTg                                                                 <  1:3803533/101‑1 (MQ=255)
                                cTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTg                                                                 <  1:3848406/101‑1 (MQ=255)
                                 tCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTgg                                                                >  2:4598244/1‑101 (MQ=255)
                                 tCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTgg                                                                >  1:422868/1‑101 (MQ=255)
                                   ggAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGc                                                              >  2:3563579/1‑101 (MQ=255)
                                     aaGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAg                                                            <  2:1205272/101‑1 (MQ=255)
                                      aGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGa                                                           <  2:3930089/101‑1 (MQ=255)
                                       gaaggCAAAAAGCCCTCCTCCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGAc                                                          <  1:2856443/98‑1 (MQ=255)
                                          ggCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCt                                                       >  2:4085671/1‑101 (MQ=255)
                                           gCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTa                                                      <  1:1083053/101‑1 (MQ=255)
                                              tctcGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACAt                                                   <  1:2963740/98‑1 (MQ=255)
                                              aaCCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACAt                                                   <  2:1605676/101‑1 (MQ=255)
                                              aaCCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACAt                                                   >  2:1483672/1‑101 (MQ=255)
                                                ccGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCa                                                 >  1:1835016/1‑101 (MQ=255)
                                                 cGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACAtaga                                                >  2:2963552/1‑98 (MQ=255)
                                                  ggCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACg                                               <  2:3072174/101‑1 (MQ=255)
                                                  ggCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACg                                               >  2:4297155/1‑101 (MQ=255)
                                                   gCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACgg                                              <  2:2871699/101‑1 (MQ=255)
                                                   gCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACgg                                              >  2:2252721/1‑101 (MQ=255)
                                                    cctcctGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGc                                             <  2:252816/101‑1 (MQ=255)
                                                     ctcctGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCg                                            <  1:3672139/101‑1 (MQ=255)
                                                     ctcctGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCg                                            >  1:840398/1‑101 (MQ=255)
                                                         tGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGCggg                                        >  1:4242335/1‑101 (MQ=255)
                                                           ctctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGaa                                      >  2:4120055/1‑101 (MQ=255)
                                                           ctctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGaa                                      <  1:4310764/101‑1 (MQ=255)
                                                           ctctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGaa                                      <  1:4662452/101‑1 (MQ=255)
                                                           ctctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGaa                                      <  1:2730215/101‑1 (MQ=255)
                                                            tctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAAc                                     <  1:4598497/101‑1 (MQ=255)
                                                             ctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACt                                    >  2:1127161/1‑101 (MQ=255)
                                                             ctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACt                                    >  1:85311/1‑101 (MQ=255)
                                                             ctTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTATCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACt                                    >  1:3307585/1‑101 (MQ=255)
                                                                tGATCATCCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTcc                                 >  1:664004/1‑101 (MQ=255)
                                                                   tcatcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCggg                              >  2:2977154/1‑101 (MQ=255)
                                                                   tcagcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGTGAACTTCCggg                              <  2:3205876/101‑1 (MQ=255)
                                                                    catcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCgggg                             >  1:785151/1‑101 (MQ=255)
                                                                    catcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCgggg                             >  2:1765627/1‑101 (MQ=255)
                                                                    catcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCgggg                             <  2:1367848/101‑1 (MQ=255)
                                                                     atcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCggggg                            >  2:4048665/1‑101 (MQ=255)
                                                                      tcCCGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTCCCctgggg                           >  2:2096435/1‑101 (MQ=255)
                                                                         cGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAg                        <  2:4363887/101‑1 (MQ=255)
                                                                         cGATGATCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAg                        >  2:2174234/1‑101 (MQ=255)
                                                                          gatgatCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAgg                       >  1:3834210/1‑101 (MQ=255)
                                                                             gatCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTca                    >  1:2395060/1‑101 (MQ=255)
                                                                             gatCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTca                    <  2:2500318/101‑1 (MQ=255)
                                                                              atCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcac                   >  1:734242/1‑101 (MQ=255)
                                                                              atCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcac                   <  1:3580747/101‑1 (MQ=255)
                                                                              atCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcac                   <  2:3479222/101‑1 (MQ=255)
                                                                              atCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcac                   >  1:175404/1‑101 (MQ=255)
                                                                              atCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcac                   >  2:639039/1‑101 (MQ=255)
                                                                               tCTGCGCCTCGGTGACCCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcaca                  <  2:4627223/101‑1 (MQ=255)
                                                                               tCTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTcaca                  <  1:2771967/101‑1 (MQ=255)
                                                                                cTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACaa                 <  2:2692682/101‑1 (MQ=255)
                                                                                cTGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACaa                 <  1:1492280/101‑1 (MQ=255)
                                                                                 tGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACaaa                <  1:4384373/101‑1 (MQ=255)
                                                                                 tGCGCCTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACaaa                <  2:2407398/101‑1 (MQ=255)
                                                                                     ccTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAAtttt            <  1:3144709/101‑1 (MQ=255)
                                                                                      cTCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTa           >  2:4251412/1‑101 (MQ=255)
                                                                                       tCGGTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACAGCGCGGGAACTTCCGGGGGGCAGGTCACAAATTTTAg          >  1:4064959/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCNGGTCACAAATTTTAGCa        >  2:1506197/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCa        >  2:1446501/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCa        >  2:3358133/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCa        >  1:2130069/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCa        >  2:2128888/1‑101 (MQ=255)
                                                                                         ggTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCa        <  2:2763342/101‑1 (MQ=255)
                                                                                          gTGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCaa       <  1:4158218/101‑1 (MQ=255)
                                                                                           tGAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCaaa      <  2:763769/101‑1 (MQ=255)
                                                                                            gAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCaaaa     >  1:1418985/1‑101 (MQ=255)
                                                                                            gAACCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCaaaa     <  2:1834907/101‑1 (MQ=255)
                                                                                              aCCGGCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCAAAAtc   >  1:2502372/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGGCAAACCGGCCTCCTGCTCTTTGATCATCCCGATGATCTGCGCCTCGGTGAACCGTCTTTTCCTCATTTAGTCTGCTCCTTCTCAGGTTGGGCAGACGCTACATCACGGCGAGGGAACTTCCGGGGGGCAGGTCACAAATTTTAGCAAAATCT  >  NC_014034/2262640‑2262835

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG