Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,330,178	G→C	100%	intergenic (+153/+159)	RCAP_rcc02161 → / ← mrcA	hypothetical protein/penicillin‑binding protein 1A

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,330,178	0	G→C	100.0%	100.9	46	intergenic (+153/+159)	RCAP_rcc02161/mrcA	hypothetical protein/penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  new base (10/34):  ref base (0/1):  total (11/35)

CTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......CG.G.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACCG  >  NC_014034/2330078‑2330245                                                                                                              |                                                                            cTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.g                                                                             <  1:426481/101‑1 (MQ=255) cTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.g                                                                             >  1:7118/1‑101 (MQ=255)  ttGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.gg                                                                            <  1:1840779/101‑2 (MQ=255)  ttGGCCCCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.gg                                                                            <  1:1117481/101‑2 (MQ=255)   tGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggc                                                                           <  1:302174/101‑3 (MQ=255)   tGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCCGGC.G.......Gg.ggg                                                                           >  2:1865817/1‑99 (MQ=255)    ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggct                                                                          <  1:1978681/101‑4 (MQ=255)    ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggcg                                                                          >  2:1953408/1‑98 (MQ=255)    ggCCTCCGCCAAGCGCGGCAACGGCGCGCAGAGCGCCGCGCGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggct                                                                          <  2:3898903/101‑4 (MQ=39)     gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctc                                                                         <  1:2753167/101‑5 (MQ=255)     gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggcgc                                                                         >  1:932480/1‑97 (MQ=255)     gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggcgc                                                                         >  2:494278/1‑97 (MQ=255)     gCCGCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctc                                                                         <  2:665626/101‑5 (MQ=255)      ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggggtc                                                                        >  2:1092243/1‑96 (MQ=255)      ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctcc                                                                        <  2:3801561/101‑6 (MQ=255)       cTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctccg                                                                       <  2:1744987/101‑7 (MQ=255)       cTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctccg                                                                       <  1:753159/101‑7 (MQ=255)        tCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggggcccc                                                                      >  2:2589074/1‑94 (MQ=255)        tCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctccgc                                                                      <  1:3085843/101‑8 (MQ=255)           gCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gg.ggctccgcccc                                                                   <  1:3475737/101‑11 (MQ=37)             cAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGAC.G.......GGCG.......CTCCGCCcccc                                                           >  2:4261945/1‑101 (MQ=255)                 ggcggcAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGCCCCGGGCGG.......GG.G.......GCCCGCCcccccccc                                                       >  1:1612493/1‑98 (MQ=255)                   cggcAACGGCGCGCAGAGCGCCGCACGGATCGCCCATTGGCGGGGGGGAATTTCGCCGCGCAGGCGCGGCGACCCGGCCGG.......GG.G.......CCCCGCCcccccacccc                                                     >  1:1345885/1‑96 (MQ=255)                               gcAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTgc                                               <  2:216482/101‑1 (MQ=18)                                  gagCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGcc                                            <  1:1519482/101‑1 (MQ=18)                                   agtgccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCa                                           <  1:2989077/98‑1 (MQ=18)                                   agCGCCGCAGGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCa                                           <  2:1982500/101‑1 (MQ=18)                                   agCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCa                                           <  2:1078889/101‑1 (MQ=18)                                    gcgcCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCaa                                          <  1:370614/101‑1 (MQ=18)                                    gcgcCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCaa                                          <  1:2948450/101‑1 (MQ=18)                                     cgccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAg                                         <  2:2985986/101‑1 (MQ=18)                                      gccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGa                                        <  2:407956/101‑1 (MQ=18)                                       ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaa                                       <  2:4380998/101‑1 (MQ=18)                                       ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaa                                       <  1:1565363/101‑1 (MQ=18)                                        cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaaa                                      <  1:3752309/101‑1 (MQ=18)                                        cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaaa                                      <  2:75073/101‑1 (MQ=18)                                         gcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCCCCCCCGGCGTATTTGCGCCAAGaaaa                                     <  2:3072877/101‑1 (MQ=18)                                          cACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaaaaa                                    <  2:4751583/101‑1 (MQ=17)                                          cACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGaaaaa                                    <  1:2985766/101‑1 (MQ=17)                                           aCGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAg                                   <  1:33983/101‑1 (MQ=17)                                           aCGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAg                                   <  1:4113351/101‑1 (MQ=17)                                           aCGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAg                                   <  2:3505186/101‑1 (MQ=17)                                            ctgATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCCGCGTATTTGCGCCAAGAAAAAGc                                  >  1:3021591/3‑101 (MQ=12)                                            cGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGc                                  >  2:2561713/1‑101 (MQ=17)                                               aTCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgg                               >  1:2244136/1‑101 (MQ=17)                                               aTCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgg                               <  1:3114109/101‑1 (MQ=18)                                               aTCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......GG.GGCTCCCCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgg                               <  1:3591565/101‑1 (MQ=18)                                                 cGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgggg                             >  1:2826356/1‑101 (MQ=17)                                                  ggCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgc                            <  2:955726/101‑1 (MQ=17)                                                  ggCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgc                            <  2:1704408/101‑1 (MQ=17)                                                  ggCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCCGCGTATTTGCGCCAAGAAAAAGCCGGGgc                            >  2:2040180/1‑101 (MQ=17)                                                   gCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.A.......CCCCGCACCCCCCGCGGATTTGCGCCAAGAAAAAGCCGGGgcg                           >  1:319659/1‑101 (MQ=16)                                                     cGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcg                         <  2:2920984/101‑1 (MQ=17)                                                        tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt                      <  1:2800457/101‑1 (MQ=17)                                                        gggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCCCCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt                      <  1:1163136/100‑1 (MQ=17)                                                           cGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCCGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATTC.g                  >  1:1865374/1‑101 (MQ=12)                                                            ggAGAGGGAGTTCCCCGCGCAGTGGCGGCGACCCGGGC.G.......GG.GGCTCCCCCCCCGCCCCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gg                 <  1:2562424/101‑1 (MQ=9)                                                              agagGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gggg               <  2:1920136/101‑1 (MQ=18)                                                              agagGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gggg               <  1:961118/101‑1 (MQ=18)                                                               gagGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGc              <  1:1865929/101‑1 (MQ=18)                                                                agGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcc             <  2:1863102/101‑1 (MQ=17)                                                                 ggAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCCCACCCCCCGCGGATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGccc            >  1:224916/1‑101 (MQ=12)                                                                  gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGGGCCG.C.......CCCCCCACCCCCCGCGGATTTGCGCCCAGAAAAAGCCGGGGGGCGCCCGC.GGGGgccc           >  2:4456391/1‑101 (MQ=9)                                                                  gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCCCACCCCCGGCGCATTTGCGCCAAGAAAAAACCCGGGCGCGCAAGC.GGGGgccc           >  2:2450071/1‑101 (MQ=9)                                                                     tttCGCCGCGCAGGCGCGGCGACCCGGGC.G.......GG.GGGTCCCCCCCCCCACCCCCCGCGGATTTTCGCCAAGAAAAAGCCGGGGGGCGCATGCGGGGGCCCCgg         >  2:488425/1‑101 (MQ=12)                                                                      ttCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGAt       <  2:4400361/101‑1 (MQ=18)                                                                      ttCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGCCGCGCATGC.GGGGCCCCGGAt       <  1:3501813/101‑1 (MQ=17)                                                                       tCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATc      <  1:238832/101‑1 (MQ=18)                                                                        cgccgcGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCa     >  2:4206489/1‑101 (MQ=18)                                                                         gccgcGCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCAc    <  2:641983/101‑1 (MQ=18)                                                                           cgcgCAGGCGCGGCGACCCGGGC.GGGGGCTCCG.C.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACcg  <  1:2995793/101‑1 (MQ=18)                                                                                                              |                                                                            CTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......CG.G.......CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACCG  >  NC_014034/2330078‑2330245 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG