Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,407,597 A→C 100% W83G (TGG→GGG)  nifH2 ← nitrogenase iron protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,407,5970A→C100.0% 770.6 201W83G (TGG→GGG) nifH2nitrogenase iron protein
Reads supporting (aligned to +/- strand):  new base (96/105):  ref base (0/0):  total (96/105)

Only 100 of 209 total aligned reads displayed.

GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694
                                                                                                   |                                                                                                 
 cgcAGCTCTTCGAGCAGATCGCCCGCGGCCGAGAGGCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaa                                                                                                <  1:639550/101‑1 (MQ=255)
  gcAGCTCTTCGAGCAGATCGCCCGCGGCCGAGATGCCG.CGCCCGGCGCACCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaaa                                                                                               <  1:4544307/101‑1 (MQ=255)
  gcAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCTCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCACCTCGATGCACAGAACGCCCCCGaaa                                                                                               >  2:1548040/1‑101 (MQ=255)
  gcAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaaa                                                                                               >  2:4031943/1‑101 (MQ=255)
  gcAGCGCTTCGAGCAGATCGCCCGCGGCCGGGACGCCG.CGCCCGGCCCAGCCCACCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaaa                                                                                               <  2:3946371/101‑1 (MQ=255)
   gaGCTCTCCGAGCAAATCGACCCGGGCTGGAATGGCG.CGCCCGGCGAACCCCACCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAc                                                                                              <  2:734273/100‑1 (MQ=255)
    aGCTCTTGGGGCAGACCGCCCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             <  1:2457545/101‑1 (MQ=255)
    aGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             >  2:1788257/1‑101 (MQ=255)
    aGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             >  2:2341618/1‑101 (MQ=255)
     gCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.TGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            <  1:4379658/101‑1 (MQ=255)
     gCTCTTCGAGCAGACCGCCCGCGGCCGAGATGCCG.CGCCCGGCGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            <  1:2012276/101‑1 (MQ=255)
       tctTCGAGCAGATCGACCGCGGCCGAGACGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGCTGCACCGAACGCCCCCGAAACAGAc                                                                                          >  1:403230/1‑101 (MQ=255)
        ctTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  2:3431975/1‑101 (MQ=255)
         ttCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcgg                                                                                        >  1:4312109/1‑101 (MQ=255)
         ttCCAGCAGATCGCCCGCGGCCGGGAGGCCG.CCCCCGCCGCGCCCCACCCCCGGCACCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcgg                                                                                        <  1:3489860/101‑1 (MQ=255)
          tCGAGCAGACCGCCCGCGGCCGAGATGCCG.CCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggc                                                                                       <  1:1602434/101‑1 (MQ=255)
           cGAGCAGATCGCCCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      <  1:1295999/101‑1 (MQ=255)
           cGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcg                                                                                      >  1:81414/1‑101 (MQ=255)
            gAGCAGATCGCCCGCGGCCGAGATCCCG.CGCCCGGCGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcgg                                                                                     <  1:359713/101‑1 (MQ=255)
            gAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcgg                                                                                     >  1:1367396/1‑101 (MQ=255)
             aGCAGATCGCCCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGa                                                                                    <  1:695579/101‑1 (MQ=255)
             aGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAATGCCCCCGAAACCGACGGCGGa                                                                                    <  1:2698705/101‑1 (MQ=255)
             aGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGa                                                                                    >  2:436999/1‑101 (MQ=255)
               cAGATCGCCCGCGGCGGAGATCCCG.CGCCCGGCGCCGCCCCCCCCCGGCACCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  <  2:2636399/101‑1 (MQ=255)
               cAGATCGCCCGCGGCCGAGATGCCG.CCCCCGGCGCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  <  2:2877698/101‑1 (MQ=255)
               cAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  >  1:4712686/1‑101 (MQ=255)
               cAGAGCGCCCGCGGCCAAGATCCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  <  2:242067/101‑1 (MQ=255)
                aGATCGACCGCGGCCGAGATGCCG.CGCCGGGCCCCGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAAt                                                                                 <  2:1371758/101‑1 (MQ=255)
                aGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAAt                                                                                 >  2:3909935/1‑101 (MQ=255)
                aGATCCCCCGGGGCCGAGACGCCG.CCCCCGGCGAAGCCCCCCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAAt                                                                                 <  1:1526577/101‑1 (MQ=255)
                 gATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTGGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATg                                                                                >  1:4064316/1‑101 (MQ=255)
                 aaccgcccGCGGCCGAGGCCCCG.CCCCCGGCGCCCCCCCCCCCCGGCCCCGGCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATg                                                                                <  2:3437961/95‑1 (MQ=255)
                  aTCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGt                                                                               >  2:332987/1‑101 (MQ=255)
                      cccGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTc                                                                           <  2:270841/100‑1 (MQ=255)
                        cgcgGCCGAGATGCCG.CGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         <  1:3430218/101‑1 (MQ=255)
                            ggcgggcgcccG.CGCCCGGCGCACCCCCCCCCCGGCCCCCCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCtt                                                                     <  1:2039236/92‑1 (MQ=255)
                             ccGAGCGGCCG.CGCCCGGCCCCCCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    <  1:2722444/101‑1 (MQ=255)
                             ccGAGATGGCGCCGCCCGGCGTAGCCCCCCCCCGGCGCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCtt                                                                     <  2:490684/101‑1 (MQ=255)
                             ccGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    >  2:3789763/1‑101 (MQ=255)
                             ccGAGATGCCC.GCCCCGGCCCACCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    <  1:1961868/101‑1 (MQ=255)
                                 gaTGCCG.CCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGtt                                                                <  1:3715063/101‑1 (MQ=255)
                                  aggCCG.CGCCGGGCCCCGCCCCCCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               <  1:522675/99‑1 (MQ=255)
                                   tGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTg                                                              >  1:2966365/1‑101 (MQ=255)
                                    cccG.CGCCCCGCTCACCCCACCCCCGGCCCCGGCCCGCCCGGCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             <  1:1517691/100‑1 (MQ=255)
                                    cccG.CCCCCGGCCCACCCCCCCCCCGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             <  1:1446209/100‑1 (MQ=255)
                                         cgcCCGGCGCACCCCACCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGc                                                         <  2:534897/101‑1 (MQ=255)
                                         ccccgggCGCACCCCCCCCCCGCCCCCGCCCTCCCCGCCTCGATGCACAGAGCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGc                                                         <  1:319367/96‑1 (MQ=255)
                                          gcCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCa                                                        >  1:1015614/1‑101 (MQ=255)
                                           cccGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCAc                                                       >  2:590903/1‑101 (MQ=255)
                                            ccggccccccccCCCCCCGCCCCCGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:3628128/92‑1 (MQ=255)
                                            ccGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  1:2878160/101‑1 (MQ=255)
                                            ccGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      >  1:1936285/1‑101 (MQ=255)
                                                cgcAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  >  1:3669867/1‑101 (MQ=255)
                                                cgcAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcg                                                  >  2:2780751/1‑101 (MQ=255)
                                                 gcAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgc                                                 >  2:155981/1‑101 (MQ=255)
                                                  cAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgcg                                                >  1:1481641/1‑101 (MQ=255)
                                                    gCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCa                                              >  1:609405/1‑101 (MQ=255)
                                                       cACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGc                                           >  1:1219974/1‑101 (MQ=255)
                                                          ccccGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGt                                        >  1:4124229/1‑101 (MQ=255)
                                                          ccccGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGt                                        >  2:2474056/1‑101 (MQ=255)
                                                           cccGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTc                                       <  1:2597058/101‑1 (MQ=255)
                                                            ccgccccctgcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTcc                                      <  2:1328322/92‑1 (MQ=255)
                                                             cggcccgcccccccccgCCTCGATGCACAGAACGCCCACGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCa                                     <  2:1249066/92‑1 (MQ=255)
                                                             ccgccccGCCCCGCCCGCCGCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCa                                     <  2:4661510/96‑1 (MQ=255)
                                                                 aCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCAc                                 >  2:1967276/1‑101 (MQ=255)
                                                                 aCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCAc                                 >  2:4518807/1‑101 (MQ=255)
                                                                 aCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGACGCCCCGGCTGTCACGCAc                                 >  2:2184420/1‑101 (MQ=255)
                                                                  ccGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACg                                >  2:3974515/1‑101 (MQ=255)
                                                                  ccGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACg                                <  1:891880/101‑1 (MQ=255)
                                                                   cGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTATGCGGGCACGGTCGCGCAGGCTGTCCAGCAccg                               >  1:4050125/1‑99 (MQ=255)
                                                                     gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  1:1786512/1‑101 (MQ=255)
                                                                     gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  2:1120744/1‑101 (MQ=255)
                                                                     gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  2:3077256/1‑101 (MQ=255)
                                                                      cccgcccgCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTgg                            <  2:2144792/101‑1 (MQ=255)
                                                                       ccgcccgcCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTggg                           <  1:2205187/101‑1 (MQ=255)
                                                                       ccgcccgcCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTggg                           >  1:4746870/1‑101 (MQ=255)
                                                                        tgcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGc                          >  1:2522298/2‑101 (MQ=255)
                                                                        cgcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGc                          <  2:1724996/101‑1 (MQ=255)
                                                                         gcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCa                         >  2:3222468/1‑101 (MQ=255)
                                                                          gctgccGCGGTGCACAGAAAGCCCCCGAAACCGACGGCGGGAGTGTCTACCCGCTGCGGTTTGCGGGCACGGTCGGGCAGGCTTTCCAGGACGGTGGGCAg                        <  2:2971066/98‑1 (MQ=255)
                                                                          cccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAg                        <  2:4064074/101‑1 (MQ=255)
                                                                            cgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGtt                      <  2:81407/101‑1 (MQ=255)
                                                                            cgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGtt                      <  1:4186916/101‑1 (MQ=255)
                                                                            cgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGtt                      <  2:2446495/101‑1 (MQ=255)
                                                                              ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  2:3669638/101‑1 (MQ=255)
                                                                              ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  1:3925752/101‑1 (MQ=255)
                                                                              ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  1:1754098/101‑1 (MQ=255)
                                                                               cTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTg                   >  1:4472567/1‑101 (MQ=255)
                                                                                   aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               <  2:4746614/101‑1 (MQ=255)
                                                                                   aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  1:3315177/1‑101 (MQ=255)
                                                                                   aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  1:4402459/1‑101 (MQ=255)
                                                                                       acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           <  1:3353197/101‑1 (MQ=255)
                                                                                       acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           >  1:4201711/1‑101 (MQ=255)
                                                                                         aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         >  2:41961/1‑101 (MQ=255)
                                                                                          gAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCgcgc        <  1:3910177/101‑1 (MQ=255)
                                                                                           aaCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCa       >  2:4483133/1‑101 (MQ=255)
                                                                                            aCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAg      <  2:2966177/101‑1 (MQ=255)
                                                                                             cGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAgg     <  2:1768549/101‑1 (MQ=255)
                                                                                                cccCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAtg  <  1:1876746/101‑1 (MQ=255)
                                                                                                cccCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAtg  <  2:2210015/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCG.CGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG