Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,003 A→G 6.5% intergenic (+51/‑191) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0030A→G 6.5% 19.6 ‑1.1 ‑0.5170intergenic (+51/‑191)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (71/88):  total (78/92)
Fisher's exact test strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.28e-01

Only 100 of 188 total aligned reads displayed.

GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTG  >  NC_014034/2493904‑2494102
                                                                                                     |                                                                                                           
 cgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcag                                                                                                            <  1:3001636/101‑1 (MQ=25)
  gTCTGGCCCAGCGCCGCGGCGGGCGCGGGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                                                                                           <  2:2276190/101‑1 (MQ=21)
  gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTgccgc                                                                                                           >  2:3190727/1‑101 (MQ=21)
      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                                                                       <  2:1483601/101‑1 (MQ=21)
       gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                                                                      <  1:3608562/101‑1 (MQ=21)
          cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                                                                   <  1:1135872/101‑1 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                  <  2:1427296/101‑1 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                  <  1:1287612/101‑1 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                  <  2:1620984/101‑1 (MQ=21)
            gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                 <  1:1190688/101‑1 (MQ=21)
             cgccgcGGCGGGCGCGCGGCACGACAGGCCAAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                <  2:2600873/101‑1 (MQ=18)
               ccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                              <  2:2728771/101‑1 (MQ=21)
                cgcgGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                             <  2:2829653/101‑1 (MQ=21)
                cgcgGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                             <  1:1867603/101‑1 (MQ=21)
                 gcggcgGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                            <  2:2655894/101‑1 (MQ=21)
                     cgggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                        <  2:902414/101‑1 (MQ=21)
                      gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                       <  1:4035747/101‑1 (MQ=21)
                      gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATCTTTGCAGCAAGATGAAAACCGAGGCCtt                                                                                       >  1:1629492/1‑101 (MQ=17)
                        gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                     <  2:455064/101‑1 (MQ=21)
                        gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                     <  2:2192603/101‑1 (MQ=21)
                        gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                     <  2:4220403/101‑1 (MQ=21)
                          gcgcgGCACGGCGGGCAGAGTGAG.GGGGGGGCGCTGCCGCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAACCCGGATGCCTTTCAt                                                                                   <  2:4382182/101‑1 (MQ=17)
                          gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                   >  1:4421786/1‑101 (MQ=21)
                          gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                   <  2:4753806/101‑1 (MQ=21)
                          gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                   >  2:4475525/1‑101 (MQ=21)
                          gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAAt                                                                                   >  1:3671805/1‑101 (MQ=18)
                           cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                  >  2:3321987/1‑101 (MQ=21)
                            gcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                 <  2:1119874/101‑1 (MQ=21)
                              ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                               <  2:3990192/101‑1 (MQ=21)
                                 aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGGAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                            >  2:2360973/1‑101 (MQ=21)
                                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCTGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                           <  2:4202202/101‑1 (MQ=21)
                                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                           >  1:2437528/1‑101 (MQ=21)
                                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                           <  2:3405855/101‑1 (MQ=21)
                                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                           >  1:944194/1‑101 (MQ=21)
                                   gACAGGCCGTGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                          <  2:45622/101‑1 (MQ=18)
                                      aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                       >  2:1270726/1‑101 (MQ=21)
                                       ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                      <  1:2629970/101‑1 (MQ=21)
                                       ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAata                                                                      >  1:848932/1‑101 (MQ=18)
                                           gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                  <  1:2875601/101‑1 (MQ=18)
                                           gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                  <  1:1960456/101‑1 (MQ=18)
                                            gaggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                  <  1:305139/94‑1 (MQ=18)
                                            aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                                                                 >  2:4149881/1‑101 (MQ=18)
                                              gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                <  2:1688279/96‑1 (MQ=255)
                                                aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                              >  2:3737533/4‑101 (MQ=255)
                                                 aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                             <  1:4354082/99‑1 (MQ=255)
                                                 aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                             >  1:1229022/3‑101 (MQ=255)
                                                 aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                             <  2:2608289/99‑1 (MQ=255)
                                                  ggggggcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                            <  1:783717/100‑1 (MQ=255)
                                                   gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                           <  1:2651728/101‑1 (MQ=255)
                                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                          >  1:1625525/1‑101 (MQ=255)
                                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                          >  2:3922708/1‑101 (MQ=255)
                                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                         >  1:4641123/1‑101 (MQ=255)
                                                      ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                        <  1:4475775/101‑1 (MQ=255)
                                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                      <  1:1520811/101‑1 (MQ=255)
                                                        ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                     <  2:3759132/101‑1 (MQ=255)
                                                            cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                              >  2:4291802/1‑101 (MQ=255)
                                                            cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                              <  1:3999310/101‑1 (MQ=255)
                                                            cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                              <  2:940000/101‑1 (MQ=255)
                                                             tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                             >  2:98397/1‑100 (MQ=255)
                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                          <  1:4605223/101‑5 (MQ=255)
                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                          <  1:1381414/101‑5 (MQ=255)
                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                          <  1:480788/101‑5 (MQ=255)
                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                          >  2:3871363/1‑97 (MQ=255)
                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AT.CAg                                         <  1:2790042/101‑1 (MQ=255)
                                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                           <  1:4551889/101‑4 (MQ=255)
                                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                           <  1:3577036/101‑4 (MQ=255)
                                                                   ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                       >  2:617701/4‑94 (MQ=255)
                                                                    cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                      <  2:2067283/99‑9 (MQ=255)
                                                                      tCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCAAGATGAAACCGGATACCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                    >  1:43063/1‑91 (MQ=18)
                                                                           aaGCGGGCTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                             <  1:2260694/101‑1 (MQ=18)
                                                                           aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                             <  1:1431276/101‑1 (MQ=18)
                                                                             gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagc                           >  1:2176995/1‑99 (MQ=18)
                                                                             gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaga                           >  1:4734439/1‑99 (MQ=18)
                                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                         >  1:4676075/1‑97 (MQ=18)
                                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                         >  2:172563/1‑97 (MQ=18)
                                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGACCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                         >  1:2769520/1‑97 (MQ=17)
                                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cc                      >  1:2725543/1‑101 (MQ=17)
                                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                     >  2:3025943/1‑101 (MQ=18)
                                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                     >  1:283443/1‑101 (MQ=18)
                                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                     >  2:2414556/1‑101 (MQ=18)
                                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCG.cccc                    <  1:402027/101‑1 (MQ=17)
                                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCG.cccc                    <  2:2954777/101‑1 (MQ=17)
                                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                    <  2:196547/101‑1 (MQ=17)
                                                                                   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                   >  1:806307/1‑101 (MQ=17)
                                                                                   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                   >  1:1565968/1‑101 (MQ=18)
                                                                                     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                 <  2:848858/101‑3 (MQ=17)
                                                                                       cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGACCCCCTGc                >  2:1978451/1‑101 (MQ=17)
                                                                                        ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccctgcc              <  2:4018385/101‑6 (MQ=17)
                                                                                         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..TAGGGGGGC.AGCGCCCCCCTGccc              >  1:2925682/1‑101 (MQ=18)
                                                                                          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc             <  1:4206111/101‑1 (MQ=0)
                                                                                          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc             >  1:3846964/1‑101 (MQ=17)
                                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg            <  2:1362966/101‑1 (MQ=0)
                                                                                            gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc           >  1:4404457/1‑100 (MQ=2)
                                                                                             atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggg          >  1:3337824/1‑100 (MQ=17)
                                                                                                tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccgga       >  2:2878051/1‑100 (MQ=2)
                                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc     >  2:263673/1‑101 (MQ=17)
                                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc     <  1:3190927/101‑1 (MQ=17)
                                                                                                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg    >  1:4463377/1‑101 (MQ=17)
                                                                                                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg  <  1:998655/101‑1 (MQ=17)
                                                                                                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg  <  1:2414691/101‑1 (MQ=17)
                                                                                                     |                                                                                                           
GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTG  >  NC_014034/2493904‑2494102

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG