Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,014 A→G 6.6% intergenic (+62/‑180) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0140A→G 6.6% 19.9 ‑0.6 ‑0.3170intergenic (+62/‑180)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (77/80):  total (84/86)
Fisher's exact test strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.72e-01

Only 100 of 187 total aligned reads displayed.

AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113
                                                                                                     |                                                                                                          
atggccggGGCGGGCGCGCGGCCCGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                            <  2:1497035/98‑1 (MQ=17)
aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                            <  2:1427296/101‑1 (MQ=21)
 gcgcCGGGGCGGGCGCGCGGCAGGCCAGACCGAAGAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                           <  1:2938237/101‑1 (MQ=14)
 gcgcCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                           <  1:1190688/101‑1 (MQ=21)
  cgccgcGGCGGGCGCGCGGCACGACAGGCCA.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                          <  2:2600873/101‑1 (MQ=18)
    ccgcGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                        <  2:2728771/101‑1 (MQ=21)
     cgcgGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                       <  1:1867603/101‑1 (MQ=21)
     cgcgGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                       <  2:2829653/101‑1 (MQ=21)
          cgggcgCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                  <  2:902414/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATCTTTGCAGCAAGATGAAAACCGAGGCCtt                                                                                                 >  1:1629492/1‑101 (MQ=17)
            ggcgCGCGGCACGACAGGCCG.AGTAAGGGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                <  2:4267536/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                               <  2:2192603/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                               <  2:455064/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                               <  1:4238216/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                               <  2:4220403/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                               <  1:1227097/101‑1 (MQ=21)
               gcgcgGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                             >  1:4421786/1‑101 (MQ=21)
               gcgcgGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAAt                                                                                             >  1:3671805/1‑101 (MQ=18)
                  cgGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                          >  2:2651574/1‑101 (MQ=21)
                  cgGCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCCGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCtt                                                                                          >  2:3335491/1‑101 (MQ=18)
                   ggCACGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                         <  2:4007606/101‑1 (MQ=21)
                       cGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCTGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                     <  2:4202202/101‑1 (MQ=21)
                       cGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                     >  1:944194/1‑101 (MQ=21)
                       cGACAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                     >  1:2437528/1‑101 (MQ=21)
                          cAGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                  >  2:2623504/1‑101 (MQ=21)
                           aGGCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                 >  2:1270726/1‑101 (MQ=21)
                            ggCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                <  2:1003329/101‑1 (MQ=21)
                            ggCCG.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                <  1:4661547/101‑1 (MQ=21)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            <  1:2875601/101‑1 (MQ=18)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            <  1:1232173/101‑1 (MQ=18)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            >  2:3439104/1‑101 (MQ=18)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            >  1:260317/1‑101 (MQ=18)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            <  1:1960456/101‑1 (MQ=18)
                                g.AGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                            >  2:3097438/1‑101 (MQ=18)
                                  aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                                                                           >  2:4149881/1‑101 (MQ=18)
                                   gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                          <  2:1688279/96‑1 (MQ=255)
                                    taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                         >  2:4753295/5‑101 (MQ=255)
                                     aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                        >  2:3737533/4‑101 (MQ=255)
                                     aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                        >  2:3228300/4‑101 (MQ=255)
                                      aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                       <  2:2608289/99‑1 (MQ=255)
                                      aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                       >  1:4201577/3‑101 (MQ=255)
                                       ggggggcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                      <  1:783717/100‑1 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                     >  1:3835710/1‑100 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                     <  1:2651728/101‑1 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                     >  2:4551779/1‑101 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                    >  1:1625525/1‑101 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                    >  2:3922708/1‑101 (MQ=255)
                                          gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                   >  1:4641123/1‑101 (MQ=255)
                                           ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                  >  1:4360479/1‑101 (MQ=255)
                                           ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                  >  1:4682235/1‑101 (MQ=255)
                                            gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                <  1:1520811/101‑1 (MQ=255)
                                             ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                               <  2:1976251/101‑1 (MQ=255)
                                             ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                               <  2:3759132/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                        <  2:940000/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                        <  1:3999310/101‑1 (MQ=255)
                                                  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                       >  2:98397/1‑100 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                    <  1:1381414/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                    >  2:3871363/1‑97 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                    <  1:480788/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                    <  1:4605223/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AT.CAg                                                   <  1:2790042/101‑1 (MQ=255)
                                                    cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                     <  1:4551889/101‑4 (MQ=255)
                                                        ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                 >  2:617701/4‑94 (MQ=255)
                                                                aaGCGGGCTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                         <  1:2260694/101‑1 (MQ=18)
                                                                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                         <  1:1431276/101‑1 (MQ=18)
                                                                  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                       >  1:2176995/1‑99 (MQ=18)
                                                                  gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCaga                                       >  1:4734439/1‑99 (MQ=18)
                                                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGACCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                     >  1:2769520/1‑97 (MQ=17)
                                                                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                 >  1:2725543/1‑101 (MQ=17)
                                                                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcggcc                                   >  2:2348947/1‑95 (MQ=17)
                                                                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cc                                >  1:283443/1‑101 (MQ=18)
                                                                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cc                                >  2:2414556/1‑101 (MQ=18)
                                                                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.c.ccc                               <  2:2954777/101‑1 (MQ=17)
                                                                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.c.ccc                               <  1:402027/101‑1 (MQ=17)
                                                                       cTCCCCCCGGGATAGTTGCAGCAAGATGAATCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccc                               <  2:43056/101‑1 (MQ=17)
                                                                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cccc                              >  1:806307/1‑101 (MQ=17)
                                                                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cccc                              >  1:1565968/1‑101 (MQ=18)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccct                            <  1:4471390/101‑3 (MQ=17)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccct                            <  2:848858/101‑3 (MQ=17)
                                                                             ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgcc                         <  2:4018385/101‑6 (MQ=17)
                                                                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCATAGGGGGGC.AGCGCC.CCCCTGccc                         >  1:2925682/1‑101 (MQ=18)
                                                                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                        >  1:3588006/1‑101 (MQ=17)
                                                                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                        <  2:191057/101‑1 (MQ=17)
                                                                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                       <  2:1362966/101‑1 (MQ=0)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcg                       <  1:4417932/101‑1 (MQ=17)
                                                                                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgccg                    >  2:1139753/1‑101 (MQ=2)
                                                                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CACCCCTGCCCcggcgg                   >  2:2029738/1‑101 (MQ=18)
                                                                                     tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgccgga                  >  2:2878051/1‑100 (MQ=2)
                                                                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                <  1:3190927/101‑1 (MQ=17)
                                                                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGt              <  1:4346247/101‑1 (MQ=17)
                                                                                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg             <  1:2414691/101‑1 (MQ=17)
                                                                                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg             <  1:998655/101‑1 (MQ=17)
                                                                                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCGCC.CCCCTGCCCCGGCGGTCCGTggc           >  1:345412/1‑101 (MQ=18)
                                                                                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGCCCGTggcg          >  1:34950/1‑101 (MQ=17)
                                                                                             aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg          <  1:836859/101‑1 (MQ=9)
                                                                                                aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGgcggcg       >  2:1882578/1‑101 (MQ=18)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc     >  1:2626861/1‑101 (MQ=17)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc     <  2:4421536/101‑1 (MQ=17)
                                                                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAAG.CCAGAGGGGGGCAGGGC.C.CCCCTGCCCCCGCGGTCAGAGGCGGCGccg    >  1:4463056/1‑100 (MQ=12)
                                                                                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg  <  1:3335693/101‑1 (MQ=17)
                                                                                                     |                                                                                                          
AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG