Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,015 C→G 6.6% intergenic (+63/‑179) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0150C→G 6.6% 19.8 ‑0.6 ‑0.2168intergenic (+63/‑179)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (77/79):  total (85/83)
Fisher's exact test strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.46e-01

Only 100 of 183 total aligned reads displayed.

GCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGA  >  NC_014034/2493916‑2494114
                                                                                                     |                                                                                                           
gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                             <  1:1190688/101‑1 (MQ=21)
 cgccgcGGCGGGCGCGCGGCACGACAGGCCAAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                            <  2:2600873/101‑1 (MQ=18)
   ccgcGGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                          <  2:2728771/101‑1 (MQ=21)
    cgcgGCGGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                         <  2:2829653/101‑1 (MQ=21)
       ggcgggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                      <  1:2775258/101‑1 (MQ=21)
          gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                   <  1:4035747/101‑1 (MQ=21)
            gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  1:1227097/101‑1 (MQ=21)
            gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  1:4238216/101‑1 (MQ=21)
            gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  1:1545937/101‑1 (MQ=21)
            gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  2:2192603/101‑1 (MQ=21)
            gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  2:4220403/101‑1 (MQ=21)
             cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                <  2:887464/101‑1 (MQ=21)
              gcgcgGCACGGCGGGCAGAGTGAG.GGGGGGGCGCTGCCGCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAACCCGGATGCCTTTCAt                                                                                               <  2:4382182/101‑1 (MQ=17)
              gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                               >  2:4475525/1‑101 (MQ=21)
              gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                               <  2:4753806/101‑1 (MQ=21)
              gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAAt                                                                                               >  1:3671805/1‑101 (MQ=18)
               cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                              <  1:1456007/101‑1 (MQ=21)
                 cgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                            >  2:2651574/1‑101 (MQ=21)
                 cgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCCGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCtt                                                                                            >  2:3335491/1‑101 (MQ=18)
                  ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                           <  2:3990192/101‑1 (MQ=21)
                      cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCTGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                       <  2:4202202/101‑1 (MQ=21)
                      cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                       >  1:2437528/1‑101 (MQ=21)
                          aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                   >  2:1270726/1‑101 (MQ=21)
                          aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                   >  1:2901878/1‑101 (MQ=21)
                           ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  <  1:4661547/101‑1 (MQ=21)
                           ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  <  2:1003329/101‑1 (MQ=21)
                           ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAata                                                                                  >  1:848932/1‑101 (MQ=18)
                            gCCGAGG.AAGGGGGGGGCGCTGCCCCCCGTNCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                 <  1:962273/101‑1 (MQ=18)
                              cGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                               >  1:2765184/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  2:3097438/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  2:3439104/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  1:260317/1‑101 (MQ=18)
                               gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              <  1:1232173/101‑1 (MQ=18)
                                gaggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              <  1:305139/94‑1 (MQ=18)
                                aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                                                                             >  2:4149881/1‑101 (MQ=18)
                                aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  2:911869/101‑1 (MQ=18)
                                  gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                            <  2:1688279/96‑1 (MQ=255)
                                   taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           >  2:4753295/5‑101 (MQ=255)
                                   taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           >  2:1381320/5‑101 (MQ=255)
                                    aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          >  2:3228300/4‑101 (MQ=255)
                                    aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          >  2:3737533/4‑101 (MQ=255)
                                    aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          >  1:347592/4‑101 (MQ=255)
                                     aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         <  2:2608289/99‑1 (MQ=255)
                                      ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  1:1430865/2‑101 (MQ=255)
                                      ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  1:4450895/2‑101 (MQ=255)
                                      ggggggcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        <  1:783717/100‑1 (MQ=255)
                                       gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                       <  1:2651728/101‑1 (MQ=255)
                                       gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                       >  2:4551779/1‑101 (MQ=255)
                                        ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                      >  1:1625525/1‑101 (MQ=255)
                                         gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                     <  1:1970580/101‑1 (MQ=255)
                                         gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                     >  1:4641123/1‑101 (MQ=255)
                                         gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                     >  1:191082/1‑101 (MQ=255)
                                          ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                    >  1:4360479/1‑101 (MQ=255)
                                          ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                    >  1:4682235/1‑101 (MQ=255)
                                           gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  >  2:788864/1‑101 (MQ=255)
                                            ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                 <  2:3759132/101‑1 (MQ=255)
                                                cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                          >  2:4291802/1‑101 (MQ=255)
                                                   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      <  1:4605223/101‑5 (MQ=255)
                                                   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      <  1:480788/101‑5 (MQ=255)
                                                   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      >  2:3871363/1‑97 (MQ=255)
                                                   cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                       <  1:4551889/101‑4 (MQ=255)
                                                   cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                       <  1:3577036/101‑4 (MQ=255)
                                                       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                   <  1:2848002/98‑8 (MQ=255)
                                                        cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                  <  2:2067283/99‑9 (MQ=255)
                                                               aaGCGGGCTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                         <  1:2260694/101‑1 (MQ=18)
                                                                 gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaga                                       >  1:4734439/1‑99 (MQ=18)
                                                                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcggcc                                   >  2:2348947/1‑95 (MQ=17)
                                                                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                                 >  2:2414556/1‑101 (MQ=18)
                                                                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCG.cccc                                <  2:2954777/101‑1 (MQ=17)
                                                                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                <  2:196547/101‑1 (MQ=17)
                                                                       tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                               >  1:1565968/1‑101 (MQ=18)
                                                                       tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                               >  1:806307/1‑101 (MQ=17)
                                                                         cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                             <  1:4471390/101‑3 (MQ=17)
                                                                           cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGACCCCCTGc                            >  2:1978451/1‑101 (MQ=17)
                                                                            ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccctgcc                          <  2:4018385/101‑6 (MQ=17)
                                                                             cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..TAGGGGGGC.AGCGCCCCCCTGccc                          >  1:2925682/1‑101 (MQ=18)
                                                                             cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGccc                          <  1:2203745/101‑1 (MQ=17)
                                                                              gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc                         <  1:4206111/101‑1 (MQ=0)
                                                                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                         >  1:3588006/1‑101 (MQ=17)
                                                                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                         >  1:3846964/1‑101 (MQ=17)
                                                                               ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg                        <  1:4015443/101‑1 (MQ=0)
                                                                               ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg                        <  2:1362966/101‑1 (MQ=0)
                                                                               ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                        <  1:4417932/101‑1 (MQ=17)
                                                                                 atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                      <  2:3882519/101‑3 (MQ=2)
                                                                                 atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggg                      >  1:3337824/1‑100 (MQ=17)
                                                                                  tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccg                     >  2:1139753/1‑101 (MQ=2)
                                                                                  tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcg                     >  1:1636751/1‑101 (MQ=18)
                                                                                    tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccgga                   >  2:2878051/1‑100 (MQ=2)
                                                                                    tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcggc                   >  2:977702/1‑100 (MQ=17)
                                                                                      tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                 >  1:3303403/1‑101 (MQ=17)
                                                                                      tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                 >  2:263673/1‑101 (MQ=17)
                                                                                       gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                >  1:4463377/1‑101 (MQ=17)
                                                                                        cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt               <  1:4346247/101‑1 (MQ=17)
                                                                                         agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg              <  1:2414691/101‑1 (MQ=17)
                                                                                            aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg           <  1:836859/101‑1 (MQ=9)
                                                                                              gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcggc         >  2:4232837/1‑101 (MQ=17)
                                                                                               aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg        >  2:1882578/1‑101 (MQ=18)
                                                                                                 gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc      >  1:2626861/1‑101 (MQ=17)
                                                                                                  aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGAGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt     >  1:2831091/1‑101 (MQ=18)
                                                                                                    aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg   >  1:2685816/1‑101 (MQ=18)
                                                                                                     |                                                                                                           
GCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGA  >  NC_014034/2493916‑2494114

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG