Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 6.4% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 6.4% 19.1 ‑0.6 ‑0.3170intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (78/81):  total (85/85)
Fisher's exact test strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.76e-01

Only 100 of 187 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A..GCAG..AAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                    |                                                                                                             
 cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGGGCTGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGGTCTTTGGCGCAAGATGAAACCggg                                                                                                              >  2:2920256/1‑100 (MQ=18)
  gcggcgGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                             <  2:2655894/101‑1 (MQ=21)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                           <  1:2775258/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                        <  1:4035747/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                        <  2:417549/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATCTTTGCAGCAAGATGAAAACCGAGGCCtt                                                                                                        >  1:1629492/1‑101 (MQ=17)
        ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                       <  2:4267536/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                      <  1:1227097/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                      <  1:1545937/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                      <  2:455064/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                      <  2:2192603/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                      <  2:4220403/101‑1 (MQ=21)
          cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                     <  2:887464/101‑1 (MQ=21)
           gcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                                    <  2:4753806/101‑1 (MQ=21)
           gcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAAt                                                                                                    >  1:3671805/1‑101 (MQ=18)
            cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                   >  2:3321987/1‑101 (MQ=21)
            cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                   <  1:1456007/101‑1 (MQ=21)
            cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATc                                                                                                   >  1:3655630/1‑101 (MQ=18)
               ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                <  2:4007606/101‑1 (MQ=21)
               ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                <  2:3990192/101‑1 (MQ=21)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCAATTTGCAGCAAGATGAAACCGGATGACTTTCATCTTGCCCg                                                                                            >  2:2847826/1‑101 (MQ=18)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                            <  2:3405855/101‑1 (MQ=21)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                            >  1:2437528/1‑101 (MQ=21)
                   cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                            >  1:944194/1‑101 (MQ=21)
                       aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                        >  1:2901878/1‑101 (MQ=21)
                       aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                        >  2:1270726/1‑101 (MQ=21)
                        ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                       <  1:4661547/101‑1 (MQ=21)
                        ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                       <  1:2629970/101‑1 (MQ=21)
                        ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAata                                                                                       >  1:848932/1‑101 (MQ=18)
                           cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                    >  1:2765184/1‑101 (MQ=18)
                            gAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                   >  1:260317/1‑101 (MQ=18)
                            gAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                   >  2:3097438/1‑101 (MQ=18)
                            gAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                   <  1:1232173/101‑1 (MQ=18)
                             aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                  <  2:911869/101‑1 (MQ=18)
                                aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                               >  1:347592/4‑101 (MQ=255)
                                aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                               >  2:2740666/4‑101 (MQ=255)
                                 aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                              <  2:2608289/99‑1 (MQ=255)
                                 aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                              <  1:4354082/99‑1 (MQ=255)
                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                             >  1:1430865/2‑101 (MQ=255)
                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                             >  1:4450895/2‑101 (MQ=255)
                                   gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                            >  2:4551779/1‑101 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                          >  1:4641123/1‑101 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                          <  1:1970580/101‑1 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                          >  1:191082/1‑101 (MQ=255)
                                      ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                         <  1:4475775/101‑1 (MQ=255)
                                      ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                         <  2:3812296/101‑1 (MQ=255)
                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                       >  2:788864/1‑101 (MQ=255)
                                        ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                      <  2:1976251/101‑1 (MQ=255)
                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTAAA.TGGc                                                                    >  1:789771/1‑101 (MQ=255)
                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                           <  1:480788/101‑5 (MQ=255)
                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                           <  2:4121073/101‑5 (MQ=255)
                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                           <  1:4605223/101‑5 (MQ=255)
                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                            <  1:3577036/101‑4 (MQ=255)
                                                ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                          <  1:2298329/101‑6 (MQ=255)
                                                   ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                        <  1:2848002/98‑8 (MQ=255)
                                                      tCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCAAGATGAAACCGGATACCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                     >  1:43063/1‑91 (MQ=18)
                                                         gCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AAGGCAG..AGggggg                                                >  1:1601862/1‑101 (MQ=18)
                                                             gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGCaga                                           >  1:4734439/1‑99 (MQ=18)
                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGCagcgc                                         >  2:172563/1‑97 (MQ=18)
                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGCagcgc                                         >  1:4676075/1‑97 (MQ=18)
                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGCagcggcc                                       >  2:2348947/1‑95 (MQ=17)
                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCG.c.cc                                    >  2:3025943/1‑101 (MQ=18)
                                                                 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCG.c.cc                                    >  2:2414556/1‑101 (MQ=18)
                                                                  cTCCCCCCGGGATAGTTGCAGCAAGATGAATCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCG.c.ccc                                   <  2:43056/101‑1 (MQ=17)
                                                                         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..TAGGGGGGC.AGCGCC.CCCCTGccc                             >  1:2925682/1‑101 (MQ=18)
                                                                         cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGccc                             <  1:2203745/101‑1 (MQ=17)
                                                                          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.A..AAGGCCAAGAGGGGGCAGCG.C.CCCCTGcccc                            <  1:4206111/101‑1 (MQ=0)
                                                                          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGcccc                            <  2:191057/101‑1 (MQ=17)
                                                                          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGcccc                            >  1:3846964/1‑101 (MQ=17)
                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcg                           >  2:3750535/1‑101 (MQ=2)
                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcg                           <  1:4015443/101‑1 (MQ=0)
                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcg                           <  2:1362966/101‑1 (MQ=0)
                                                                           ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCcg                           <  1:4417932/101‑1 (MQ=17)
                                                                            gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcgc                          >  1:4404457/1‑100 (MQ=2)
                                                                             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcgcc                         <  2:3882519/101‑3 (MQ=2)
                                                                             atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCcggg                         >  1:3337824/1‑100 (MQ=17)
                                                                              tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcgccg                        >  2:1139753/1‑101 (MQ=2)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCcggcg                        >  1:1636751/1‑101 (MQ=18)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                       >  2:1445592/1‑101 (MQ=18)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCG.CACCCCTGCCCcggcgg                       >  2:2029738/1‑101 (MQ=18)
                                                                                tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..GGCC..AAGAGGGGGCAGCG.C.CCCCTGCCCcgccgga                      >  2:2605858/1‑100 (MQ=2)
                                                                                tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCcggcggc                      >  2:977702/1‑100 (MQ=17)
                                                                                  tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                    >  1:3303403/1‑101 (MQ=17)
                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                    >  2:263673/1‑101 (MQ=17)
                                                                                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                   >  1:4463377/1‑101 (MQ=17)
                                                                                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                 <  1:2414691/101‑1 (MQ=17)
                                                                                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                 <  1:998655/101‑1 (MQ=17)
                                                                                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc         <  2:4421536/101‑1 (MQ=17)
                                                                                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGG.AAGCGCC.CCCCTGCCCCGGCGGTCCGGGGCGGGGcc         >  2:3683987/1‑101 (MQ=17)
                                                                                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGAGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        >  1:2831091/1‑101 (MQ=18)
                                                                                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      >  1:2685816/1‑101 (MQ=18)
                                                                                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      >  1:812992/1‑101 (MQ=17)
                                                                                                 ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa     <  1:2623656/101‑1 (MQ=17)
                                                                                                  ccgATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  2:1774723/99‑1 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  2:4640867/101‑1 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  2:1041092/101‑1 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    >  1:769470/1‑101 (MQ=18)
                                                                                                   ggATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGTTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg   <  1:4552032/101‑1 (MQ=17)
                                                                                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg   >  1:1223598/1‑101 (MQ=17)
                                                                                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCA..GAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg   >  1:3101667/1‑101 (MQ=17)
                                                                                                    |                                                                                                             
CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A..GCAG..AAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG