BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,061	G→A	6.4%	intergenic (+109/‑133)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,061	0	G→A	6.4%	20.0 ‑1.1 ‑1.2	158	intergenic (+109/‑133)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (7/3): ref base (72/75): total (79/79)
Fisher's exact test strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.86e-02

Only 100 of 167 total aligned reads displayed.


CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAG..A.AGGGGGGCA.AGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493962‑2494152


cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                    >  2:4291802/1‑101 (MQ=255)


cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                    <  1:3999310/101‑1 (MQ=255)


 tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                                   >  2:98397/1‑100 (MQ=255)


   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                                >  2:3871363/1‑97 (MQ=255)


   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                                <  1:1381414/101‑5 (MQ=255)


   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.T.CAg                                                                                              <  1:2790042/101‑1 (MQ=255)


   cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                                 <  1:3577036/101‑4 (MQ=255)


   cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                                 <  1:4551889/101‑4 (MQ=255)


       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                             >  2:617701/4‑94 (MQ=255)


       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                             <  1:2848002/98‑8 (MQ=255)


        cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                            <  2:2067283/99‑9 (MQ=255)


                aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGCag                                                                                <  1:4753551/101‑2 (MQ=18)


                 gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGCagc                                                                               >  1:2176995/1‑99 (MQ=18)


                 gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGCaga                                                                               >  1:4734439/1‑99 (MQ=18)


                    gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.c                                                                        >  1:2725543/1‑101 (MQ=17)


                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGCagcggcc                                                                           >  2:2348947/1‑95 (MQ=17)


                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.cc                                                                       >  2:2414556/1‑101 (MQ=18)


                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.cc                                                                       >  1:283443/1‑101 (MQ=18)


                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG....AGGGGGGC..AGCG.c.ccc                                                                      <  1:402027/101‑1 (MQ=17)


                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG....AGGGGGGC..AGCG.c.ccc                                                                      <  2:2954777/101‑1 (MQ=17)


                       tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.cccc                                                                     >  1:1565968/1‑101 (MQ=18)


                         cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.ccccct                                                                   <  1:4471390/101‑3 (MQ=17)


                           cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGAC.CCCCTGc                                                                  >  2:1978451/1‑101 (MQ=17)


                            ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.c.ccccctgcc                                                                <  2:4018385/101‑6 (MQ=17)


                             cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..T.AGGGGGGC..AGCGCC.CCCCTGccc                                                                >  1:2925682/1‑101 (MQ=18)


                             cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGccc                                                                <  1:2203745/101‑1 (MQ=17)


                              gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.A.A.AGGCCA.AGAGGGGGC.AGCG.C.CCCCTGcccc                                                               <  1:4206111/101‑1 (MQ=0)


                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGcccc                                                               <  2:191057/101‑1 (MQ=17)


                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGcccc                                                               >  1:3588006/1‑101 (MQ=17)


                               ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCC..A.AGAGGGGGC.AGCG.C.CCCCTGCCCcg                                                              >  1:659612/1‑101 (MQ=2)


                               ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCC..A.AGAGGGGGC.AGCG.C.CCCCTGCCCcg                                                              <  2:1362966/101‑1 (MQ=0)


                                gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCC..A.AGAGGGGGC.AGCG.C.CCCCTGCCCcgc                                                             >  1:4404457/1‑100 (MQ=2)


                                  tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCC..A.AGAGGGGGC.AGCG.C.CCCCTGCCCcgccg                                                           >  2:1139753/1‑101 (MQ=2)


                                  tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCcggcg                                                           >  1:1636751/1‑101 (MQ=18)


                                   atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCG.CACCCCTGCCCcggcgg                                                          >  2:2029738/1‑101 (MQ=18)


                                      tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       >  1:3303403/1‑101 (MQ=17)


                                      tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       >  2:263673/1‑101 (MQ=17)


                                      tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       <  1:3190927/101‑1 (MQ=17)


                                        cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGt                                                     <  1:4346247/101‑1 (MQ=17)


                                         agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTg                                                    <  1:2414691/101‑1 (MQ=17)


                                           caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                                                  <  1:2524071/101‑1 (MQ=17)


                                           caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGG.A.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                                                  >  1:345412/1‑101 (MQ=18)


                                              gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTggcggc                                               >  2:4232837/1‑101 (MQ=17)


                                                 gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            <  2:4421536/101‑1 (MQ=17)


                                                 gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGG.A.AGCGCC.CCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                            >  2:3683987/1‑101 (MQ=17)


                                                  aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAA.G.CCA..G.AGGGGGGCA.GGGC.C.CCCCTGCCCCCGCGGTCAGAGGCGGCGccg                                           >  1:4463056/1‑100 (MQ=12)


                                                   aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  1:4650818/101‑1 (MQ=17)


                                                    aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:3335693/101‑1 (MQ=17)


                                                    aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         >  1:812992/1‑101 (MQ=17)


                                                     ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  1:2623656/101‑1 (MQ=17)


                                                      ccgATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:1774723/99‑1 (MQ=17)


                                                      cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:1041092/101‑1 (MQ=17)


                                                      cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  1:769470/1‑101 (MQ=18)


                                                       ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg                                      >  1:1223598/1‑101 (MQ=17)


                                                       ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  1:3101667/1‑101 (MQ=17)


                                                        gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     <  1:2311185/101‑1 (MQ=17)


                                                         aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGCAGAGC..C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGatcgg                                    >  2:857681/1‑97 (MQ=17)


                                                         aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  1:1172984/101‑1 (MQ=17)


                                                         aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:1430771/101‑1 (MQ=17)


                                                             cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  2:84451/1‑101 (MQ=17)


                                                              tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  1:4605986/101‑1 (MQ=17)


                                                              tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:944117/101‑1 (MQ=17)


                                                              tttCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:4386003/101‑1 (MQ=17)


                                                               ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  2:1718966/1‑101 (MQ=18)


                                                                tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                             >  2:3581/1‑100 (MQ=18)


                                                                tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  2:2564012/101‑1 (MQ=18)


                                                                 cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  1:3806231/1‑101 (MQ=18)


                                                                  aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggggg                           >  1:4009401/1‑98 (MQ=17)


                                                                   tCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:2176871/101‑1 (MQ=18)


                                                                   tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          >  2:4376916/1‑101 (MQ=18)


                                                                   tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          >  1:46093/1‑101 (MQ=18)


                                                                    cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  2:1633663/1‑101 (MQ=18)


                                                                      tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGGCCACGAAGGGCCGGGGCGCgggg                       >  1:835590/1‑100 (MQ=9)


                                                                      tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       <  1:617765/101‑1 (MQ=18)


                                                                      tGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       <  2:4637959/101‑1 (MQ=18)


                                                                       gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  2:4636090/1‑101 (MQ=18)


                                                                        cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  2:3538976/101‑1 (MQ=18)


                                                                         ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCAtt                    >  1:2628535/1‑101 (MQ=18)


                                                                         ccGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:2122/101‑1 (MQ=18)


                                                                           gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  1:98413/101‑1 (MQ=18)


                                                                           gAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGCCCTTCACGGGCGGGGGGGCGGGCATTaa                  >  2:1959993/1‑101 (MQ=12)


                                                                            aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  2:4734183/101‑1 (MQ=18)


                                                                            aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  1:459589/101‑1 (MQ=18)


                                                                             aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  2:90304/1‑101 (MQ=21)


                                                                             aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                <  1:2740830/101‑1 (MQ=21)


                                                                             aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:1127494/1‑101 (MQ=21)


                                                                              atatCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               >  1:1296638/1‑101 (MQ=21)


                                                                               taccccGGGGGGTGAA.TGGC..G.A.A.GCC..A.GAGGGGGCC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              <  2:1329142/98‑1 (MQ=21)


                                                                                atCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:1228239/1‑101 (MQ=21)


                                                                                atCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             <  1:2278845/101‑1 (MQ=21)


                                                                                atCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGG.G.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGCGCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  1:538066/1‑101 (MQ=16)


                                                                                atCCCGGGGGGGGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGaa             >  2:2821257/1‑101 (MQ=21)


                                                                                 tCCCGGGGGGTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:4150127/101‑1 (MQ=21)


                                                                                      gggggTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:4292049/101‑1 (MQ=25)


                                                                                      gggggTGAA.TGGC..G.AGG.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  2:417052/101‑1 (MQ=25)


                                                                                        gggTGAA.TGGC..GAA.GCCAG....AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     >  1:3130257/1‑101 (MQ=25)


                                                                                        gggTGAA.TGGC..GAA.G.CCA..G.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:2590566/101‑1 (MQ=25)


                                                                                        gggTGAA.TGGC..G.A.A.GAG..AGAGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  2:71212/101‑1 (MQ=37)


                                                                                         ggTGAA.TGGC..GAA.GCCAG....AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCaaaa    >  1:16817/1‑99 (MQ=21)


                                                                                         ggTGAA.TGGC..GAA.GCCAG....AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  2:377256/1‑101 (MQ=25)


CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAG..A.AGGGGGGCA.AGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493962‑2494152

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG